Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,470 Δ1 bp 2.1% intergenic (+73/+75) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4700C→. 2.1% 4.5 ‑1.2 ‑0.1189intergenic (+73/+75)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (71/114):  total (74/115)
Fisher's exact test strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.77e-01

Only 100 of 214 total aligned reads displayed.

CTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGC  >  NC_014034/432371‑432570
                                                                                                     |                                                                                                    
cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                                                                      <  1:142804/101‑1 (MQ=255)
cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                                                                      <  2:537867/101‑1 (MQ=255)
 tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATcc                                                                                                     <  1:4621467/101‑1 (MQ=255)
  ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                                                                    <  1:414656/101‑1 (MQ=255)
   tgggcggcgacggGTCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATccttt                                                                                                  <  2:3230350/99‑4 (MQ=14)
   tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCgg                                                                                                   <  1:4064213/101‑1 (MQ=255)
      gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                                                <  2:2385753/101‑1 (MQ=255)
       cgggggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                               <  2:1351289/101‑1 (MQ=255)
         gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                                             <  2:3441428/101‑1 (MQ=255)
          cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                                            <  2:625054/101‑1 (MQ=255)
           ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                           <  1:639621/101‑1 (MQ=255)
             ggggCCACGCCTGAGGCGGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                                         <  1:1792129/100‑1 (MQ=255)
               ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                       <  2:1607290/101‑1 (MQ=255)
               ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                       <  2:4950907/101‑1 (MQ=255)
                 ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCt                                                                                     <  2:686553/101‑1 (MQ=255)
                     gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                                                                 <  1:3436511/101‑1 (MQ=255)
                     gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                                                                 <  1:3203470/101‑1 (MQ=255)
                     gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                                                                 >  1:2418904/1‑101 (MQ=255)
                       cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                               >  1:2894562/1‑101 (MQ=255)
                        tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                                                              <  1:925358/101‑1 (MQ=255)
                        tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                                                              <  2:1086873/101‑1 (MQ=255)
                        tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                                                              >  2:2798863/1‑101 (MQ=255)
                        tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                                                              <  1:1192728/101‑1 (MQ=255)
                        tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                                                              >  1:1954379/1‑101 (MQ=255)
                         gAGGCCGGGCAGGGGGGCGCTGGCCCCCGTCC.TGCGGACTCCCCCCGGGACATTTGAGGCAAGATGAAAGCCGATCCGGGCTTAGTTTCATGTTGTCCCaa                                                                             >  2:4492459/1‑101 (MQ=18)
                         gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAGAGCCGATCCGGGCTTCGTTTCATCTTGCCCCaa                                                                             >  1:3501807/1‑101 (MQ=255)
                         gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCaa                                                                             >  2:3227401/1‑101 (MQ=255)
                         gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCaa                                                                             <  1:2142989/101‑1 (MQ=255)
                         gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCaa                                                                             <  2:5007869/101‑1 (MQ=255)
                          gggCCGGGCGGGGGGGGGCTGCCCCCCTC.C.CGCCGGCCCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                                           <  1:2749055/100‑1 (MQ=21)
                          aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCaaa                                                                            <  2:3022136/101‑1 (MQ=255)
                           ggCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                                           <  2:140511/101‑1 (MQ=255)
                            gCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAata                                                                          <  1:245528/101‑1 (MQ=255)
                            gCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAata                                                                          <  2:3530945/101‑1 (MQ=255)
                              cGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATc                                                                        <  1:545878/101‑1 (MQ=255)
                                ggacggggggCTCTGCCCCCCGG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt                                                           >  2:2709821/5‑101 (MQ=2)
                                 gCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCCTCTTGCCCCAAATATCCCg                                                                     >  1:489739/1‑101 (MQ=255)
                                  acggggggCTCTGCCCCCCGG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttgg                                                         >  2:4596727/3‑99 (MQ=2)
                                   aGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                   >  1:3138566/1‑101 (MQ=255)
                                     gggggCTCTGCCCCCCGG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggcca                                                      >  1:4786312/1‑96 (MQ=2)
                                     gggggCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggggg                                                                 >  2:3098637/1‑101 (MQ=255)
                                      ggggCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGAATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt                                                                <  2:3302149/101‑1 (MQ=255)
                                      ggggCGCTGCCCCCCGT.CGTGCGGACACCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCAGGGGt                                                                <  2:3379116/101‑1 (MQ=25)
                                       gggCGCTTCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                               <  2:4432724/101‑1 (MQ=255)
                                       gggCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                               <  2:3485013/101‑1 (MQ=255)
                                       gggCGCCGCCCCCCGTCC.TGCGGGCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                               <  1:3539895/101‑1 (MQ=25)
                                         gcgcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGaa                                                             <  1:1771721/101‑1 (MQ=255)
                                         cgccgcccccccTC.C.TGCGGACCCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                                                            <  1:175571/95‑1 (MQ=25)
                                          cgcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                                                            >  2:3170758/1‑101 (MQ=255)
                                          cgcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                                                            <  2:4203244/101‑1 (MQ=255)
                                            cTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                                                          <  2:54148/101‑1 (MQ=255)
                                            cTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                                                          >  1:4212722/1‑101 (MQ=255)
                                            cTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                                                          <  1:1878669/101‑1 (MQ=255)
                                            cTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAAttt                                                          >  1:4690159/1‑101 (MQ=255)
                                              gCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                                                        <  2:325722/101‑1 (MQ=255)
                                               ccccccGTCC.TGCGGACTCCCCCGGGGGTATTTGACGCTACATGAAAGCCGATCAGGGCTTCGTTTCATCTTGCCCCAAATATACGGGGGGGGAAttttag                                                       >  2:4041419/1‑99 (MQ=25)
                                                   ccgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCa                                                   <  1:2609886/96‑1 (MQ=255)
                                                    cgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAg                                                  <  1:3959461/97‑1 (MQ=255)
                                                     gtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                 <  1:1579785/98‑1 (MQ=255)
                                                     gtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                 >  1:842326/4‑101 (MQ=255)
                                                      gcccggCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                 <  1:4402337/96‑1 (MQ=255)
                                                              gACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCCAAagag                                         >  2:2545597/1‑101 (MQ=255)
                                                                  ccccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggggg                                     <  2:4286450/101‑1 (MQ=255)
                                                                   cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                    <  2:4689836/101‑1 (MQ=255)
                                                                    ctccGGGATTTTTGAGGCAAGATGAAAGCCGCTCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGCTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                   <  1:4492779/99‑1 (MQ=255)
                                                                     cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGTCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                  <  2:2487214/101‑1 (MQ=255)
                                                                      ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                 <  2:1049039/101‑1 (MQ=255)
                                                                       cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                <  1:3099163/101‑1 (MQ=255)
                                                                       cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                <  1:2725105/101‑1 (MQ=255)
                                                                         ggATATTTGAGGCAGGATGAAAGCCGATCCGGGTTTCTTTCAATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                              <  2:4110435/101‑1 (MQ=21)
                                                                         ggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                              >  1:2912269/1‑101 (MQ=255)
                                                                          gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                             <  1:4113650/101‑1 (MQ=255)
                                                                            tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                           <  2:3934374/101‑1 (MQ=255)
                                                                            tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                           >  2:3748885/1‑101 (MQ=255)
                                                                             atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                          >  2:312182/1‑101 (MQ=255)
                                                                             atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                          >  1:1357953/1‑101 (MQ=255)
                                                                              tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                         >  1:90165/1‑101 (MQ=255)
                                                                              tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                         >  2:1482006/1‑101 (MQ=255)
                                                                              tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                         >  2:3958219/1‑101 (MQ=255)
                                                                               ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                        <  2:3828209/101‑1 (MQ=255)
                                                                                  nggNANNATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                     <  1:2332813/100‑1 (MQ=255)
                                                                                  aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                     <  2:5045661/101‑1 (MQ=255)
                                                                                  aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                     >  1:2212981/1‑101 (MQ=255)
                                                                                   ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                    >  2:1264735/1‑101 (MQ=255)
                                                                                     cAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgc                  >  2:4266985/1‑101 (MQ=255)
                                                                                      aaGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg                 >  1:3379658/1‑101 (MQ=255)
                                                                                       aGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg                >  2:2636105/1‑101 (MQ=255)
                                                                                          tGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgc             >  1:4986220/1‑101 (MQ=255)
                                                                                           gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg            <  2:4212405/101‑1 (MQ=255)
                                                                                           gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg            >  2:1208970/1‑101 (MQ=255)
                                                                                           gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg            >  2:229648/1‑101 (MQ=255)
                                                                                            aaaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg           >  1:1505094/1‑101 (MQ=255)
                                                                                            aaaGCCAACCCTCGCGTCGTTTCACCTTGCCCAAAATATCCCGGGGGTGAATTGTGGCGCAGCCAAAAGAGGGGCCAGCCCCCCCTATCCCcgcggcgcgg           <  2:2037117/101‑1 (MQ=21)
                                                                                             aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc          >  1:4020882/1‑101 (MQ=255)
                                                                                             aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc          >  2:825711/1‑101 (MQ=255)
                                                                                                 cGATCCGGGCATCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcg      >  1:4367326/1‑101 (MQ=255)
                                                                                                  gATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc     >  1:2677332/1‑101 (MQ=255)
                                                                                                    tCCGGGCTTCGTTTCATCTTGCCCCAAATACCCCGGGCGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGcggcgcggcggcgcgg   <  1:4262361/101‑1 (MQ=255)
                                                                                                     ccGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCggcgcggcggcgcggc  <  1:4761883/101‑1 (MQ=255)
                                                                                                     ccGGGCTTCGTTTAATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCggcgcggcggcgcggc  >  1:2354436/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
CTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGC  >  NC_014034/432371‑432570

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG