Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 735,234 Δ1 bp 1.8% intergenic (‑140/‑345) bchF ← / → ppaA 2‑vinyl bacteriochlorophyllide hydratase/regulatory protein PpaA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034735,2340C→. 1.8% 4.2 ‑1.1 ‑0.6217intergenic (‑140/‑345)bchF/ppaA2‑vinyl bacteriochlorophyllide hydratase/regulatory protein PpaA
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (96/116):  total (100/117)
Fisher's exact test strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.35e-01

Only 100 of 261 total aligned reads displayed.

AAAACTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGCG  >  NC_014034/735134‑735333
                                                                                                     |                                                                                                   
   aCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.cccc                                                                                                  <  1:5015994/101‑1 (MQ=255)
    cTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.ccccc                                                                                                 >  2:3408274/1‑101 (MQ=255)
    cTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.ccccc                                                                                                 >  2:3955743/1‑101 (MQ=255)
    cTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.ccccc                                                                                                 <  1:1843938/101‑1 (MQ=255)
     tGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.cccccc                                                                                                >  1:2556049/1‑101 (MQ=255)
     tGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.cccccc                                                                                                <  2:1976427/101‑1 (MQ=255)
      ggTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.ccccccc                                                                                               <  2:4395174/101‑1 (MQ=255)
       gTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.cccccccc                                                                                              >  2:513826/1‑101 (MQ=255)
         cTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCg                                                                                            >  1:3783760/1‑101 (MQ=255)
           tGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGc                                                                                          >  2:3061898/1‑101 (MQ=255)
           tGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGc                                                                                          >  1:4028839/1‑101 (MQ=255)
            gTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCt                                                                                         <  1:1628527/101‑1 (MQ=255)
             tCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTa                                                                                        <  1:3408537/101‑1 (MQ=255)
             tCACGAGAAAGTGTCAATGAAAACTTACACTCACGGGCGGGGCGAAACGCCGCGGTCCTTGCGCGCAAGGGCGCCTTGTTGCAGCGG.ACCGCCCCCGGcga                                                                                        >  2:1764691/1‑99 (MQ=255)
              ctcGAGAAAGTGTCAATGAAAACATACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCCTGATGCAGCGG.CCCCCCCCCGGCTaa                                                                                       <  2:1415593/99‑1 (MQ=255)
              cACGAGAAAGTGTCAATGAAAACTTGCACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTaa                                                                                       <  2:3237185/101‑1 (MQ=255)
              cACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTaa                                                                                       <  2:578325/101‑1 (MQ=255)
                cGAGAAAGTGTCAATGAAAACTTACACTCAGGGGGGGGACGAAATGCCGCTGGCCTTGCGCCCAAGGGCGCCTTGATGCAGCGG.CTCCCCCCCgaatgaac                                                                                     >  1:4186299/1‑94 (MQ=255)
                cGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTCAAc                                                                                     >  2:727207/1‑101 (MQ=255)
                cGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAAc                                                                                     <  1:407704/101‑1 (MQ=255)
                cGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAAc                                                                                     <  1:2425932/101‑1 (MQ=255)
                 gagaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAAcc                                                                                    <  1:1523713/101‑1 (MQ=255)
                 gagaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGAGG.CCCCCCCCCGGCTAAAcc                                                                                    >  1:707165/1‑101 (MQ=255)
                     aaGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCgg                                                                                >  2:2791366/1‑101 (MQ=255)
                       gtgtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTc                                                                              <  1:4430959/101‑1 (MQ=255)
                       gtgtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTc                                                                              >  1:1957816/1‑101 (MQ=255)
                       gtgtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTc                                                                              <  1:883761/101‑1 (MQ=255)
                        tgtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCa                                                                             <  2:4047214/101‑1 (MQ=255)
                         gtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCAc                                                                            <  2:2985142/101‑1 (MQ=255)
                         gtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCAc                                                                            <  1:4730744/101‑1 (MQ=255)
                          tCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTGGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCCCGGTCACg                                                                           <  2:1897468/101‑1 (MQ=255)
                            aaTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGAc                                                                         >  2:761834/1‑101 (MQ=255)
                             aTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGAcc                                                                        >  1:3905176/1‑100 (MQ=255)
                             aTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACt                                                                        >  2:4708458/1‑101 (MQ=255)
                             aTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACt                                                                        <  1:1944776/101‑1 (MQ=255)
                             aTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACt                                                                        <  2:3829237/101‑1 (MQ=255)
                              tGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTc                                                                       <  2:906006/101‑1 (MQ=255)
                               gAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCg                                                                      >  2:3605179/1‑101 (MQ=255)
                               gAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCg                                                                      <  1:538000/101‑1 (MQ=255)
                                aaaaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGt                                                                     >  2:2484588/1‑101 (MQ=255)
                                aaaaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGCCACGCCTCGt                                                                     >  1:2822279/1‑101 (MQ=255)
                                 aaaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTc                                                                    >  2:3472102/1‑101 (MQ=255)
                                 aaaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTc                                                                    <  1:844035/101‑1 (MQ=255)
                                   aCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCg                                                                  <  2:185676/101‑1 (MQ=255)
                                   aCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCg                                                                  <  1:3887914/101‑1 (MQ=255)
                                      tACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGc                                                               <  1:2294903/101‑1 (MQ=255)
                                       acacTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCTCCGGCTAAACCGCGGTCACGACTCGTCCGGGcc                                                              >  1:4353493/1‑100 (MQ=255)
                                         acTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAAACCCGGGCACGACTCGTCCcgggcga                                                            >  1:4633555/1‑97 (MQ=255)
                                          cTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaa                                                           <  1:1336043/101‑1 (MQ=255)
                                           tCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaaa                                                          <  2:1702815/101‑1 (MQ=255)
                                           tCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaaa                                                          >  1:1278606/1‑101 (MQ=255)
                                             aGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAcc                                                        >  2:1046347/1‑101 (MQ=255)
                                              ggggCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcg                                                       >  2:4808527/1‑101 (MQ=255)
                                              ggggCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcg                                                       >  2:4472880/1‑101 (MQ=255)
                                              ggggCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcg                                                       >  2:4222113/1‑101 (MQ=255)
                                               gggCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgg                                                      <  1:2171437/101‑1 (MQ=255)
                                                ggCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcggg                                                     >  1:1208890/1‑101 (MQ=255)
                                                ggCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcggg                                                     >  1:1400133/1‑101 (MQ=255)
                                                 gCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggc                                                    <  2:2320990/101‑1 (MQ=255)
                                                   gggACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcgg                                                  <  1:3308936/101‑1 (MQ=255)
                                                         aaaCGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGgcggggcggg                                            >  2:119536/1‑101 (MQ=255)
                                                            ccccccTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATg                                         <  1:3548488/96‑1 (MQ=255)
                                                             gccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATgc                                        >  2:1494676/1‑101 (MQ=255)
                                                             gccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATgc                                        <  1:54156/101‑1 (MQ=255)
                                                              ccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATgcg                                       <  2:3546352/101‑1 (MQ=255)
                                                              ccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATgcg                                       <  1:3100962/101‑1 (MQ=255)
                                                               cgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCATGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATgcgc                                      <  1:1802618/101‑1 (MQ=255)
                                                                  tGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCtt                                   <  2:2239756/101‑1 (MQ=255)
                                                                  tGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCtt                                   <  2:1441784/101‑1 (MQ=255)
                                                                  cgCCCCTGCCCGCAAGGGCCCCTTGGTGGAGCGGCCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCt                                    <  2:1387379/100‑1 (MQ=255)
                                                                   gCCCTTGCGCGCAAGGGCGCCTTGATGCAGAGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGAGGCGCCttt                                  >  1:4492160/1‑101 (MQ=255)
                                                                    cccTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCtttt                                 >  1:2653078/1‑101 (MQ=255)
                                                                     ccTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAGCCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTtc                                <  2:3722521/101‑1 (MQ=255)
                                                                     ccTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTtc                                <  2:3916106/101‑1 (MQ=255)
                                                                      cTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTtct                               <  1:3820083/101‑1 (MQ=255)
                                                                       ttggccgcAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTtctc                              <  2:2999570/96‑1 (MQ=255)
                                                                        tGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCCCGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGAGGCGCCTTTtttcc                             >  1:2950920/1‑100 (MQ=255)
                                                                        tGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCa                             <  2:2369316/101‑1 (MQ=255)
                                                                         gcgcgcAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCaa                            <  1:2449466/101‑1 (MQ=255)
                                                                          cgcgcAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAAc                           <  1:1866298/101‑1 (MQ=255)
                                                                          cgcgcAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAAc                           <  1:477964/101‑1 (MQ=255)
                                                                            cgcAAGGGGGCCTTGATCCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCt                         <  1:1020449/101‑1 (MQ=255)
                                                                            cgcAAGGGCGCGTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCt                         <  2:2682834/101‑1 (MQ=255)
                                                                            cgcAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCt                         >  1:468258/1‑101 (MQ=255)
                                                                             gcAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGCCTTTTTTTCACCTg                        >  2:968300/1‑101 (MQ=255)
                                                                              cAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCTGGGCGGGATGCGCCTTTTCTCAACCTgg                       >  1:2695224/1‑101 (MQ=255)
                                                                              cAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGCCTTTTCTCAACCCgg                       >  1:1001453/1‑101 (MQ=255)
                                                                                aGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGcc                     <  2:137745/101‑1 (MQ=255)
                                                                                 gggCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGGGCCTTTTCTCAACCTGGccc                    >  1:2129634/1‑101 (MQ=255)
                                                                                   gcgcCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGt                  <  2:3783475/101‑1 (MQ=255)
                                                                                        ttGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCtt             <  2:2522674/101‑1 (MQ=255)
                                                                                        ttGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCtt             <  1:1123731/101‑1 (MQ=255)
                                                                                        ttGAGGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCtt             <  2:4633232/101‑1 (MQ=255)
                                                                                         tcaTGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTg            <  2:4142082/99‑1 (MQ=255)
                                                                                         tGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGGGGGGTGCGCCCTTTTTCCACCTGGCCCGTaatatc            >  2:2559002/1‑96 (MQ=255)
                                                                                         tGATGCAGCGG..CCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGc           >  1:643475/1‑101 (MQ=255)
                                                                                          gATGCAGCGG.CCCCCCCCCGGCTAAGCCGCGGTCACGACGCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGc           <  2:4738940/101‑1 (MQ=255)
                                                                                               cgCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgtcgt      <  2:4316653/100‑1 (MQ=255)
                                                                                                gCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTg     <  2:4502212/101‑1 (MQ=255)
                                                                                                gCGA.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTg     <  2:4832644/101‑1 (MQ=255)
                                                                                                     |                                                                                                   
AAAACTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGCG  >  NC_014034/735134‑735333

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG