BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,173,783	+C	1.9%	intergenic (+16/‑818)	RCAP_rcc01097 → / → RCAP_rcc01099	hypothetical protein/IS4 family transposase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,173,783	1	.→C	1.9%	4.5 ‑1.1 ‑1.2	213	intergenic (+16/‑818)	RCAP_rcc01097/RCAP_rcc01099	hypothetical protein/IS4 family transposase
Reads supporting (aligned to +/- strand): new base (3/1): ref base (97/112): total (100/113)
Fisher's exact test strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.77e-02

Only 100 of 266 total aligned reads displayed.


GGTCGTCGATGTGACCGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGACAATCCCGG  >  NC_014034/1173683‑1173883


     tcgATGTGACCGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.ccccc                                                                                                 <  2:1345699/101‑1 (MQ=255)


      cgATGTGACCGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGGGTTGTGGGGGGGCAGGGGCGCTG.cccccc                                                                                                >  2:3591008/1‑101 (MQ=255)


       gATGTGACCGGCTGGCTCGGCGGCTACAACTTCCCGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGGCCTG.ccccccc                                                                                               >  1:1469007/1‑101 (MQ=255)


       gATGTGACCGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.ccccccc                                                                                               <  2:900808/101‑1 (MQ=255)


       gATGTGACCGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.ccccccc                                                                                               >  2:703930/1‑101 (MQ=255)


       gATGTGACCGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGAGTTGTGAGCGGGCAGGGGCGCTG.ccccccc                                                                                               >  1:327678/1‑101 (MQ=255)


         tgtgACCGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.ccccccccc                                                                                             >  2:1253190/1‑101 (MQ=255)


         tgtgACCGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.ccccccccc                                                                                             >  1:3816389/1‑101 (MQ=255)


         tgtgACCGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.ccccccccc                                                                                             >  1:4786299/1‑101 (MQ=255)


          gtgCCCGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCct                                                                                            <  1:1370095/101‑1 (MQ=255)


           tgACCGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCctc                                                                                           <  1:2091409/101‑1 (MQ=255)


            gACCGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCctct                                                                                          <  2:3257194/101‑1 (MQ=255)


            gACCGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCctct                                                                                          >  2:2217109/1‑101 (MQ=255)


             gccGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCtt                                                                                         <  2:4794297/100‑1 (MQ=255)


              ccGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTg                                                                                        <  1:2763353/101‑1 (MQ=255)


              ccGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTg                                                                                        >  2:1681834/1‑101 (MQ=255)


              ccGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTg                                                                                        <  2:509701/101‑1 (MQ=255)


               cGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTgg                                                                                       <  1:753300/101‑1 (MQ=255)


                ggctggctCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGc                                                                                      >  1:2740482/1‑101 (MQ=255)


                ggctggctCGGCGGCTACAACTTCCAGTGGGCCGGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGc                                                                                      <  1:4378798/101‑1 (MQ=255)


                 gctggctCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGcc                                                                                     <  2:4120300/101‑1 (MQ=255)


                  ctggctCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCt                                                                                    <  1:10721/101‑1 (MQ=255)


                  ctggctCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCt                                                                                    <  2:3135398/101‑1 (MQ=255)


                    ggctCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGc                                                                                  <  1:1653987/101‑1 (MQ=255)


                    ggctCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGc                                                                                  >  1:4953149/1‑101 (MQ=255)


                     gctCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCg                                                                                 >  1:227505/1‑101 (MQ=255)


                     gctCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCCGCg                                                                                 >  1:28960/1‑101 (MQ=255)


                      ctCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCgg                                                                                >  1:3464388/1‑100 (MQ=255)


                       tCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCgtg                                                                               <  1:2333574/101‑1 (MQ=255)


                          gcggcTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCa                                                                            <  1:2561646/101‑1 (MQ=255)


                           cggcTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCAt                                                                           <  1:3713312/101‑1 (MQ=255)


                            ggcTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATc                                                                          >  1:1084261/1‑101 (MQ=255)


                             gcTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCt                                                                         <  2:1179320/101‑1 (MQ=255)


                              cTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCtt                                                                        >  1:430031/1‑101 (MQ=255)


                              cTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCtt                                                                        <  1:4647181/101‑1 (MQ=255)


                               tACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTTCGTGTCGTTttt                                                                       >  2:1068905/1‑101 (MQ=255)


                                aCAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTc                                                                      >  1:1454462/1‑101 (MQ=255)


                                aCAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTc                                                                      <  2:2742372/101‑1 (MQ=255)


                                  aaCTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCt                                                                    >  2:2510525/1‑101 (MQ=255)


                                   aCTTCCAGGGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCtt                                                                   <  1:4855393/101‑1 (MQ=255)


                                       ccAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTcc                                                               >  2:1613352/1‑101 (MQ=255)


                                       ccAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTcc                                                               >  2:1522516/1‑101 (MQ=255)


                                         aGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGGGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTcccc                                                             >  1:4723703/1‑101 (MQ=255)


                                          gTGGGCTGGGT.CTCGGGCTGGGGGGGGGCGCAGGGGTTGGGGGGGGGGCGGGGGCCTGCCCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCg                                                            <  1:906664/101‑1 (MQ=255)


                                            gggCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCa                                                          >  2:3311140/1‑101 (MQ=255)


                                              gCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAAc                                                        <  1:1253311/101‑1 (MQ=255)


                                               gcTGGTCCTCGGCCTGGGGGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACg                                                       <  2:2937729/100‑1 (MQ=255)


                                               ccTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACg                                                       >  2:3780821/1‑101 (MQ=255)


                                                cTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGt                                                      >  1:1521955/1‑101 (MQ=255)


                                                  ggTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTcc                                                    >  1:198612/1‑101 (MQ=255)


                                                  ggTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTcc                                                    >  1:4116631/1‑101 (MQ=255)


                                                   gTCCTCGGCCTGGGCGGGGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCct                                                   <  2:496260/101‑1 (MQ=255)


                                                   gTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCct                                                   <  2:3816104/101‑1 (MQ=255)


                                                   gTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCct                                                   <  2:73693/101‑1 (MQ=255)


                                                     ccTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCctct                                                 >  1:3880744/1‑101 (MQ=255)


                                                      cTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCtctc                                                <  1:3679077/101‑1 (MQ=255)


                                                      cTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCtctc                                                <  1:2280699/101‑1 (MQ=255)


                                                      cTCGGCCCGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCtctc                                                <  1:2175448/101‑1 (MQ=255)


                                                        cGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGt                                              >  2:2842658/1‑101 (MQ=255)


                                                         ggCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTg                                             >  2:2176596/1‑101 (MQ=255)


                                                            cTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGt                                          >  1:3523538/1‑101 (MQ=255)


                                                            cTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGt                                          >  1:2965689/1‑101 (MQ=255)


                                                             tGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTc                                         <  2:4403813/101‑1 (MQ=255)


                                                               ggcggcggcGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTa                                       >  1:1853524/1‑101 (MQ=255)


                                                                gcggcggcgCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTAt                                      <  1:2518900/101‑1 (MQ=255)


                                                                 cggcggcgCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATg                                     <  2:392207/101‑1 (MQ=255)


                                                                      gcgcAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCgg                                <  1:531949/101‑1 (MQ=255)


                                                                        gcAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGaa                              >  1:377823/1‑101 (MQ=255)


                                                                           ggTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTg                           >  2:246300/1‑101 (MQ=255)


                                                                            gtgtTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAAGGTCCTCTCGTGGGTCTATGACCGGAAGTGc                          <  1:4495764/101‑1 (MQ=255)


                                                                            gtgtTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGc                          >  2:1702127/1‑101 (MQ=255)


                                                                            gtgtTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTCGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGc                          >  1:5000516/1‑101 (MQ=255)


                                                                             tgttgtGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGcc                         <  1:1795272/101‑1 (MQ=255)


                                                                             tgttgtGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGGCTATGACCGGAAGTGcc                         >  2:3633732/1‑101 (MQ=255)


                                                                                 gtgAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTc                     >  2:1105824/1‑101 (MQ=255)


                                                                                 gtgAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTc                     >  1:3971774/1‑101 (MQ=255)


                                                                                 gtgAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTc                     <  1:3634004/101‑1 (MQ=255)


                                                                                  tgAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCa                    <  2:4463226/101‑1 (MQ=255)


                                                                                    aGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAAt                  >  1:4808044/1‑101 (MQ=255)


                                                                                     gCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATc                 >  2:3420988/1‑101 (MQ=255)


                                                                                        ggCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATcccc              >  1:3756386/1‑99 (MQ=255)


                                                                                         gCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCa             >  2:3962676/1‑101 (MQ=255)


                                                                                          cAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAg            <  2:646134/101‑1 (MQ=255)


                                                                                           aGGGGCGCTG.CCTCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGa           >  1:2734558/1‑101 (MQ=255)


                                                                                           aGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCACCGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGa           >  2:4206703/1‑101 (MQ=255)


                                                                                           aGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGa           >  2:572833/1‑101 (MQ=255)


                                                                                           aGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGa           >  2:2279792/1‑101 (MQ=255)


                                                                                            ggggCGCTGCCCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCaca           >  1:1164504/1‑99 (MQ=255)


                                                                                            ggggCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGAc          >  2:1977263/1‑101 (MQ=255)


                                                                                              ggCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGACaa        <  2:429966/101‑1 (MQ=255)


                                                                                              ggCGCTC.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGACaa        <  2:4574677/101‑1 (MQ=255)


                                                                                               gcgcTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGACAAt       <  2:4723380/101‑1 (MQ=255)


                                                                                               gcgcTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGACAAt       <  1:4050458/101‑1 (MQ=255)


                                                                                               gcccTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGACCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGACAAt       <  2:2069298/98‑1 (MQ=255)


                                                                                                cgcTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGACAATc      <  2:1647343/101‑1 (MQ=255)


                                                                                                 gcTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGACAATcc     <  1:2423838/101‑1 (MQ=255)


                                                                                                  cTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGACAATccc    >  1:3352716/1‑101 (MQ=255)


                                                                                                  cTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGACAATccc    <  2:4022386/101‑1 (MQ=255)


                                                                                                    g.CCCCCCCCCTCTTGGCCTGCGTGTCCTCTTTCCTTGTCCCCCCAACCTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGACAATGCCgg  >  2:4844680/1‑101 (MQ=255)


                                                                                                    g.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGACAATCCCgg  <  2:3504333/101‑1 (MQ=255)


GGTCGTCGATGTGACCGGCTGGCTCGGCGGCTACAACTTCCAGTGGGCCTGGTCCTCGGCCTGGGCGGCGGCGCAGGTGTTGTGAGCGGGCAGGGGCGCTG.CCCCCCCCCTCTTGGCCTGCGTGTCATCTTTCCTTGTCCCCGCAACGTCCTCTCGTGGGTCTATGACCGGAAGTGCCGGTCAATCCGCAGACAATCCCGG  >  NC_014034/1173683‑1173883

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 24 ≤


ATCG

< 32 ≤


ATCG

< 39 ≤


ATCG