Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,255,904 +GT 100% intergenic (+74/+168) RCAP_rcc01184 → / ← RCAP_rcc01185 CsbD family protein/methyl‑accepting chemotaxis sensory transducer

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,255,9041.→G100.0% 75.7 31intergenic (+74/+168)RCAP_rcc01184/RCAP_rcc01185CsbD family protein/methyl‑accepting chemotaxis sensory transducer
Reads supporting (aligned to +/- strand):  new base (10/21):  ref base (0/0):  total (10/21)
*NC_0140341,255,9042.→T100.0% 74.8 31intergenic (+74/+168)RCAP_rcc01184/RCAP_rcc01185CsbD family protein/methyl‑accepting chemotaxis sensory transducer
Reads supporting (aligned to +/- strand):  new base (10/21):  ref base (0/0):  total (10/21)

AGGGGTGCCGGACCGGAACCGGGGGCCTTCGG....CTG.TCTCGGTCCGCCCGAAGAGGGGGGGCGCTGTGGTAC..CCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCCGGGGGGGGGGAACT  >  NC_014034/1255834‑1255999
                                                                            ||                                                                                               
aGGGGTGCCGGACCGGAGCCGGGGGCTTTCGGGCCCCCGCTTTCGGTCCGGCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCGCCGGGat                                                                          <  2:587429/101‑1 (MQ=0)
      gCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCCTCCCTGCGGGGCCCCCCCCCGGGTATTTcc                                                                    >  1:3072193/1‑101 (MQ=2)
                                 cccccgctttcGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGcgcg                                         <  1:2176047/92‑1 (MQ=17)
                                  ccccgctttcGGTCCGCCCGAAGAGGGGGGGCGCTTCCCCCCGTACCTGCGGGACTCCCCCCGGGATGTTTCCGCCGAGTGGAAAAGAACCCGGGGCGCgg                                        >  2:2406975/9‑101 (MQ=9)
                                     cgctttcGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCtt                                     <  1:4393825/96‑1 (MQ=18)
                                      gctttcGGTCCTCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCttt                                    <  1:1870925/97‑1 (MQ=17)
                                      gctttcGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCttt                                    <  1:245022/97‑1 (MQ=18)
                                      gctttcGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCttt                                    >  1:3513186/5‑101 (MQ=18)
                                        tttcGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCa                                  >  2:1754781/3‑101 (MQ=18)
                                         ttcGGTCCGCCCGAAGAGGGGGGGCGCGGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCAc                                 <  2:2679468/100‑1 (MQ=18)
                                          tcGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCAcc                                >  2:2435586/1‑101 (MQ=18)
                                          tcGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCAcc                                <  1:2051458/101‑1 (MQ=18)
                                           ctgTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCt                               >  2:2083498/3‑101 (MQ=18)
                                            ggTCCGCCCGAAGAGGGGGGGCGCTGCCCCNCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCtt                              <  1:2354761/101‑1 (MQ=21)
                                            ggTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCtt                              >  2:3428714/1‑101 (MQ=18)
                                            ggTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCtt                              <  1:92121/101‑1 (MQ=18)
                                            ggTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCtt                              >  1:1066288/1‑101 (MQ=18)
                                            ggTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCtt                              <  1:16308/101‑1 (MQ=18)
                                            ggTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCtt                              >  1:2037799/1‑101 (MQ=18)
                                            ggTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCtt                              >  1:2264675/1‑101 (MQ=18)
                                             gTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTg                             >  1:2940988/1‑101 (MQ=18)
                                             gTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTg                             <  1:4460789/101‑1 (MQ=18)
                                              tCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTgg                            <  1:3094146/101‑1 (MQ=18)
                                              tCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTgg                            <  1:3689456/101‑1 (MQ=18)
                                               ccGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTggg                           >  2:2001218/1‑101 (MQ=18)
                                               ccGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTggg                           <  2:4297639/101‑1 (MQ=18)
                                                cGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGc                          <  2:1346129/101‑1 (MQ=18)
                                                  cccGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCa                        <  1:2015757/101‑1 (MQ=18)
                                                   ccGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCaa                       <  2:1860024/101‑1 (MQ=18)
                                                   ccGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCaa                       <  2:2376940/101‑1 (MQ=18)
                                                    cGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCaaa                      <  2:1119881/101‑1 (MQ=18)
                                                    cGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCaaa                      <  1:627349/101‑1 (MQ=18)
                                                    cGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCaaa                      <  1:4952804/101‑1 (MQ=18)
                                                    cGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCaaa                      <  2:380016/101‑1 (MQ=18)
                                                                      ccccgc..cccTGCGGGCCTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCCGGGGGGGGGGAACt  <  2:3501353/96‑1 (MQ=255)
                                                                            ||                                                                                               
AGGGGTGCCGGACCGGAACCGGGGGCCTTCGG....CTG.TCTCGGTCCGCCCGAAGAGGGGGGGCGCTGTGGTAC..CCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCCGGGGGGGGGGAACT  >  NC_014034/1255834‑1255999

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG