Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,868 +G 2.4% intergenic (+55/+102) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,8681.→G 2.4% 3.0 0.0 ‑0.6127intergenic (+55/+102)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (1/2):  ref base (55/69):  total (56/71)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.46e-01

Only 100 of 159 total aligned reads displayed.

ACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....GCCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCC  >  NC_014034/1303770‑1303961
                                                                                                       |                                                                                                    
aCAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCCCCCCCCGGATATTTGGGGCAAGAt                                                                                                       >  1:2393164/1‑101 (MQ=37)
aCAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAt                                                                                                       <  2:2556925/101‑1 (MQ=37)
aCAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAt                                                                                                       >  1:1654152/1‑101 (MQ=37)
aCAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAt                                                                                                       <  2:4691503/101‑1 (MQ=37)
aCAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAt                                                                                                       <  1:4644027/101‑1 (MQ=37)
 cAGCCTGTCCCGCGAACCCCTTCGCGCCGTGGTCAGCGTTTAGACAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.a                                                                                                     <  2:4501604/101‑1 (MQ=25)
 cAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGGTGC.CCACT.CCGCTTTCCCTCACCCCCCGGATATTTGGGGCGAGGT.a                                                                                                     >  1:474132/1‑101 (MQ=21)
 cAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.a                                                                                                     <  2:1431411/101‑1 (MQ=37)
  aGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.aa                                                                                                    <  2:4978264/101‑1 (MQ=37)
  aGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.aa                                                                                                    <  1:3872944/101‑1 (MQ=37)
  aGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.aa                                                                                                    >  2:4164588/1‑101 (MQ=37)
   gCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.aaa                                                                                                   <  2:2456186/101‑1 (MQ=37)
   gCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.aaa                                                                                                   <  1:873045/101‑1 (MQ=37)
    ccTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAc                                                                                                  <  2:2506662/101‑1 (MQ=37)
      tGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTAACCCCCGGGATATTTGGGGCAAGAT.AAAacc                                                                                                >  2:4119791/1‑101 (MQ=25)
       gTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAcccc                                                                                               >  1:3599635/1‑101 (MQ=37)
       gTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAcccc                                                                                               >  1:1513233/1‑101 (MQ=37)
         cccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTGC.CCACT.CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCAt                                                                                             <  1:779683/101‑1 (MQ=37)
         cccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAACCCCAt                                                                                             >  2:4491750/1‑101 (MQ=37)
          ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATc                                                                                            <  1:136320/101‑1 (MQ=37)
          ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTACGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATc                                                                                            <  1:4305759/101‑1 (MQ=25)
           cgcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATcc                                                                                           <  2:3370006/101‑1 (MQ=37)
           cgcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATcc                                                                                           <  2:2560248/101‑1 (MQ=37)
           cgcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCGG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATcc                                                                                           <  1:4120108/101‑1 (MQ=37)
            gcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCt                                                                                          <  1:3097592/101‑1 (MQ=37)
             cgacgcccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCGCCCCCGGGATATTTGGGGCAAGAT.AAACCCCacgctt                                                                                         <  2:4278019/96‑6 (MQ=21)
             cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCtt                                                                                         <  1:2770195/101‑1 (MQ=37)
             cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCtt                                                                                         <  2:1534879/101‑1 (MQ=37)
              gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCttt                                                                                        <  1:4433901/101‑1 (MQ=37)
              gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCttt                                                                                        <  1:453545/101‑1 (MQ=37)
              gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCttt                                                                                        <  2:1874461/101‑1 (MQ=37)
               aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGTCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGGCATTTGGGGCCAGAT.AAAACCCCTCCTTTc                                                                                       >  2:3836067/1‑101 (MQ=255)
               aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGGTATTTGGGGCAAGAT.AAACCCCATCCTTTc                                                                                       >  1:1714016/1‑101 (MQ=37)
               aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTc                                                                                       <  1:3426634/101‑1 (MQ=37)
               aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCCGGACATTTGGGGGAAGAT.AAACCCCATCCTTTc                                                                                       >  2:4879946/1‑101 (MQ=37)
                aaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCa                                                                                      <  1:3155284/101‑1 (MQ=37)
                aaCCCGGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCa                                                                                      <  2:1019405/101‑1 (MQ=25)
                  cccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCAcc                                                                                    <  1:2282758/101‑1 (MQ=37)
                  cccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCAcc                                                                                    <  2:499402/101‑1 (MQ=37)
                   ccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCCGCCCCCC..CCGCTTCGCCTCCCCCCCGGGAGATTTGGGGCGAGAT.AAACCCCATCCCTTCCCCt                                                                                   >  1:2528308/1‑101 (MQ=255)
                    cTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCCCCtt                                                                                  >  2:3471029/1‑101 (MQ=25)
                    cTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCtt                                                                                  <  2:1154462/101‑1 (MQ=37)
                     tGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTg                                                                                 <  2:3170047/101‑1 (MQ=37)
                       cgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGcc                                                                               >  1:628753/1‑101 (MQ=37)
                       cgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGcc                                                                               <  2:1068435/101‑1 (MQ=37)
                       cgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGcc                                                                               <  2:739164/101‑1 (MQ=37)
                           ccGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCCCCCCCCGGGTATTTGGGGGCAGAA.AAAACCCATCCTTTCCCCTTGCCCCaa                                                                           >  1:1016729/1‑101 (MQ=19)
                           ccGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCaa                                                                           <  2:50355/101‑1 (MQ=37)
                           ccGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCaa                                                                           <  1:3483218/101‑1 (MQ=37)
                           ccGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCaa                                                                           <  2:1105203/101‑1 (MQ=37)
                            cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCaaa                                                                          <  1:3141813/101‑1 (MQ=37)
                             gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCCCCCCCGGGATATTTGGGGGAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAat                                                                         >  1:1870780/1‑101 (MQ=37)
                             gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAat                                                                         <  1:3544100/101‑1 (MQ=37)
                             gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAat                                                                         <  1:2037190/101‑1 (MQ=37)
                               ggTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCCTCCTTTCACCTTGCCGCAAatat                                                                       >  1:2326328/1‑101 (MQ=25)
                               ggTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAatat                                                                       <  1:3211516/101‑1 (MQ=37)
                               ggTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAatat                                                                       <  1:2003797/101‑1 (MQ=37)
                                gTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATc                                                                      <  1:541335/101‑1 (MQ=37)
                                 tCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGTCGCAAATATcc                                                                     <  2:1798106/101‑1 (MQ=25)
                                 tCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATcc                                                                     <  1:2187074/101‑1 (MQ=37)
                                  cAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATccc                                                                    >  1:2845405/1‑101 (MQ=25)
                                   aGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCg                                                                   <  2:4613149/101‑1 (MQ=37)
                                     cGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                 <  1:4611885/101‑1 (MQ=37)
                                     cGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCCGGGGATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTTCCGCAAATATCCCcgg                                                                 >  1:953635/1‑101 (MQ=25)
                                        ttAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCgggggg                                                              <  2:4746623/101‑1 (MQ=37)
                                         tAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGGTATTTGGGGCAAGAT.AAACCCCCTCCTTTCACCTTGCCGCAAATATACCggggggg                                                             >  2:2881245/1‑100 (MQ=25)
                                          cggacggggggCTCTGC.CCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAAt                                                         <  1:3009559/95‑1 (MQ=2)
                                          aGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCCTCCTTTCACCTTGCCGCCAATATCCCgggggggg                                                            >  2:3564486/1‑99 (MQ=25)
                                          aGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg                                                            >  2:4205323/1‑101 (MQ=25)
                                            gCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCCAATATCCCGGGGGGTGaa                                                          >  1:4439375/1‑101 (MQ=25)
                                            gCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                          <  2:917955/101‑1 (MQ=37)
                                             cAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt                                                         >  1:2000597/1‑101 (MQ=25)
                                             cAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt                                                         <  1:3340037/101‑1 (MQ=37)
                                             cAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt                                                         <  2:2093535/101‑1 (MQ=37)
                                             cAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAAt                                                         >  1:3575335/1‑101 (MQ=25)
                                              aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                        <  2:2826704/101‑1 (MQ=37)
                                              aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                        <  2:2113989/101‑1 (MQ=37)
                                              aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAAtt                                                        >  1:5245/1‑101 (MQ=25)
                                               aGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                       <  2:624745/101‑1 (MQ=37)
                                                gggggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                      >  1:4852736/1‑101 (MQ=37)
                                                 ggggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgc                                                     >  1:2282175/1‑101 (MQ=37)
                                                 ggggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGgc                                                     >  1:4870076/1‑101 (MQ=25)
                                                                                           ttGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcgg             >  1:2037447/1‑101 (MQ=9)
                                                                                            tGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg            >  1:365845/1‑101 (MQ=14)
                                                                                            tGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg            >  1:4907550/1‑101 (MQ=14)
                                                                                            tGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg            >  2:4985056/1‑101 (MQ=14)
                                                                                             ggggCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcgggc           >  2:697684/1‑101 (MQ=14)
                                                                                             ggggCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcgggc           >  2:3751666/1‑101 (MQ=14)
                                                                                              gggCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc          >  1:493808/1‑101 (MQ=14)
                                                                                                 cAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca       >  1:1184411/1‑101 (MQ=14)
                                                                                                 cAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGCGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCggggcgca       >  2:4968101/1‑101 (MQ=9)
                                                                                                  aaGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcag      >  2:3885296/1‑101 (MQ=14)
                                                                                                   aGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg     >  2:4655654/1‑101 (MQ=14)
                                                                                                   aGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcacg     >  2:3073160/1‑99 (MQ=9)
                                                                                                    gAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcaggg    >  2:3961022/1‑101 (MQ=14)
                                                                                                    gAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcaggg    >  2:714119/1‑101 (MQ=9)
                                                                                                      t.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc  >  1:4066059/1‑101 (MQ=14)
                                                                                                      t.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc  >  2:2340659/1‑101 (MQ=14)
                                                                                                      t.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc  >  2:820898/1‑101 (MQ=14)
                                                                                                      t.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGAGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc  >  1:4324610/1‑101 (MQ=9)
                                                                                                       |                                                                                                    
ACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....GCCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCC  >  NC_014034/1303770‑1303961

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG