Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,320,707 +G 100% intergenic (+165/+20) potI1 → / ← RCAP_rcc01247 polyamine ABC transporter permease PotI/membrane transport family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,320,7071.→G100.0% 734.8 232intergenic (+165/+20)potI1/RCAP_rcc01247polyamine ABC transporter permease PotI/membrane transport family protein
Reads supporting (aligned to +/- strand):  new base (138/94):  ref base (0/0):  total (138/94)

Only 100 of 265 total aligned reads displayed.

TCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAA.GGC.A.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.G.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCGACGCCGCC  >  NC_014034/1320609‑1320802
                                                                                                      |                                                                                                 
tCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAc                                                                                                    <  2:3618760/101‑1 (MQ=37)
tCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAc                                                                                                    >  2:509722/1‑101 (MQ=37)
tCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAc                                                                                                    >  1:2540192/1‑101 (MQ=37)
 cACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g                                                                                                  >  2:1984235/1‑101 (MQ=25)
 cACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g                                                                                                  >  1:4472479/1‑101 (MQ=25)
 cACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g                                                                                                  >  2:2680893/1‑101 (MQ=25)
 cACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g                                                                                                  >  2:4435414/1‑101 (MQ=25)
 cACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g                                                                                                  >  1:2316539/1‑101 (MQ=25)
   ccccccGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g.gg                                                                                               <  1:503040/101‑1 (MQ=25)
     ccccGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g.gggg                                                                                             >  1:3147589/1‑101 (MQ=25)
     ccccGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g.gggg                                                                                             >  2:250303/1‑101 (MQ=25)
        cGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g.gggggca                                                                                          >  1:878801/1‑98 (MQ=21)
          aGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g.gggggcagc                                                                                        <  1:3988585/101‑6 (MQ=21)
          aGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g.gggggcagc                                                                                        <  2:3883714/101‑6 (MQ=21)
          aGGATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g.gggggcagc                                                                                        >  1:2406191/1‑96 (MQ=21)
           ggATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAgcg                                                                                       >  2:1733421/1‑101 (MQ=18)
            gATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAgcgc                                                                                      >  1:1917734/1‑101 (MQ=18)
            gATATTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAgcgc                                                                                      >  1:825435/1‑101 (MQ=18)
              tatTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGccc                                                                                    <  1:179245/101‑1 (MQ=18)
               atTTGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGcccc                                                                                   >  1:482071/1‑101 (MQ=18)
                tttGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGccccc                                                                                  >  2:1893761/1‑101 (MQ=18)
                 ttGAAGCAAGGTGAAACTGAAAGGGCGG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCt                                                                                 <  1:162553/101‑1 (MQ=17)
                  tGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCtt                                                                                <  2:713452/101‑1 (MQ=18)
                  tGAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.G.GGGGCCAGCGCCCCCtt                                                                                >  2:3176950/1‑101 (MQ=18)
                   gAAGCAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTTCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTg                                                                               <  1:1331970/101‑1 (MQ=17)
                       cAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTa                                                                           >  1:1416564/1‑101 (MQ=18)
                       cAAGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCAAAAATATCCCGGGGGGAGTCCCGCAGGGAC.GTGGGG.CAGCGCACCCTTGCTTa                                                                           >  2:1188106/1‑101 (MQ=18)
                        aaGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTAc                                                                          >  2:644684/1‑101 (MQ=18)
                        aaGGTGAAACTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTAc                                                                          >  2:1450924/1‑101 (MQ=18)
                               aaCTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGa                                                                   >  1:1428064/1‑101 (MQ=18)
                                aCTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGag                                                                  >  2:4227190/1‑100 (MQ=18)
                                 cTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATc                                                                 <  2:3452345/101‑1 (MQ=18)
                                 cTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATc                                                                 >  1:4166686/1‑101 (MQ=18)
                                 cTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATc                                                                 <  1:3217612/101‑1 (MQ=18)
                                 cTGAAAGGGC.AGGGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATc                                                                 <  1:303619/101‑1 (MQ=18)
                                    gaaagggcaggggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATtgc                                                               >  2:4072190/11‑101 (MQ=18)
                                     aaagggcaggggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATcgcg                                                              <  2:4661225/92‑1 (MQ=18)
                                     aaagggcaggggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATcgcg                                                              >  1:2814538/10‑101 (MQ=18)
                                     aaagggcaggggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATcgcg                                                              <  2:4344704/92‑1 (MQ=18)
                                      aagggcaggggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGa                                                             >  2:1492002/9‑101 (MQ=18)
                                        gggcaggggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGc                                                           >  1:1391246/7‑101 (MQ=18)
                                         ggcaggggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGGTCGCGGGcc                                                          >  2:4745562/6‑101 (MQ=18)
                                           caggggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAg                                                        <  2:3896133/98‑1 (MQ=255)
                                            aggggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGGTCGCGCGCCAgc                                                       >  1:710630/3‑100 (MQ=18)
                                            aggggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGa                                                       >  2:4761936/3‑101 (MQ=255)
                                            aggggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGa                                                       >  2:3219927/3‑101 (MQ=255)
                                             ggggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCCAGCCAGAc                                                      >  1:4037870/2‑101 (MQ=255)
                                              gggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGAcc                                                     <  1:3567696/101‑1 (MQ=255)
                                               ggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGGGCCAGAccc                                                    >  1:3881845/1‑101 (MQ=255)
                                               ggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCCGAccc                                                    >  2:588659/1‑101 (MQ=255)
                                               ggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGAccc                                                    <  1:4121202/101‑1 (MQ=255)
                                               ggCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGAccc                                                    >  2:2640607/1‑101 (MQ=255)
                                                gctgcgctgcCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGGTTGCGGGCCCGACCCa                                                   >  2:3563714/1‑101 (MQ=18)
                                                gctgcgctgcCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCa                                                   <  1:3745746/101‑1 (MQ=255)
                                                  tgcgctgcCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGGTCGCGAGCCCGACCCAGa                                                 >  1:1045587/1‑101 (MQ=18)
                                                   gcgctgcCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCCAGCCAGACCCAGac                                                >  2:2459115/1‑100 (MQ=255)
                                                    cgctgcCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGGGCCAGACCCCGATc                                               >  1:2026622/1‑101 (MQ=18)
                                                     gctgcCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCg                                              <  2:2803138/101‑1 (MQ=255)
                                                       tgcCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGAc                                            <  2:2271315/101‑1 (MQ=255)
                                                        gcCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACa                                           >  2:2602432/1‑101 (MQ=255)
                                                         cccTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAg                                          <  1:4450138/101‑1 (MQ=255)
                                                           cttctacTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGa                                        <  2:1588977/95‑1 (MQ=21)
                                                            ttCATCTTGCCACAAATATCCCGGGGGGGGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATTGCGGGCCCGACCCCCATCGACaaggt                                       >  2:5021669/1‑97 (MQ=17)
                                                            ttCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGAt                                       >  2:3150628/1‑101 (MQ=255)
                                                             tCATCTTGCCACAAATATCCCGGGGGGAGTCCGGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATc                                      <  2:1037189/101‑1 (MQ=255)
                                                             tCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCCGATCGACAGGATc                                      >  1:2680349/1‑101 (MQ=255)
                                                             tCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATc                                      >  1:3349436/1‑101 (MQ=255)
                                                               aTCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGGGCCAGACCCAGATCGCCAGGaccca                                    >  2:1385349/1‑97 (MQ=18)
                                                               aTCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCCGACCCAGATCGACAGGATCga                                    >  2:3115329/1‑101 (MQ=255)
                                                                tCTTGCCACAAATATCGCGGGGGGAGTACCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCgag                                   <  1:1671503/101‑1 (MQ=25)
                                                                 cTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCgaga                                  <  2:2346939/101‑1 (MQ=255)
                                                                   tcccACAAATACCCGGGGGGGAGCCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAgc                                <  1:2585699/99‑1 (MQ=25)
                                                                   tGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAgc                                <  2:1083320/101‑1 (MQ=255)
                                                                   tGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAgc                                >  1:2169640/1‑101 (MQ=255)
                                                                    gCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAAATCGACAGGATCGAGAgcg                               >  1:2861821/1‑101 (MQ=255)
                                                                        caAATATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCCGATCGACAGGATCGAGAGCGCggg                           >  1:1149336/1‑100 (MQ=255)
                                                                         aaaTATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCCGACCCAGATCGACAGGGTCGAGAGGGCgggg                          >  2:1945059/1‑99 (MQ=25)
                                                                          aaTATCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGGgg                         >  1:3915882/1‑101 (MQ=255)
                                                                             atCCCGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCaa                      <  2:825333/101‑1 (MQ=255)
                                                                               cccGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCCGACCCAGATCGACAGGATCGAGAGGGCGGGGGCAATc                    >  2:909935/1‑101 (MQ=37)
                                                                               cccGGGGGGAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATc                    <  1:2726031/101‑1 (MQ=255)
                                                                                 gggggggAGCCCCGCAGGGGCGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAg                  <  1:1855779/100‑1 (MQ=38)
                                                                                  ggggggAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCCGACCCAGATCGACAGGATCGCGAGCGCGGCgggacacacc                 >  2:4277992/1‑93 (MQ=25)
                                                                                  ggggggAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGGGGCAATCaac                 >  2:2143755/1‑99 (MQ=255)
                                                                                  ggggggAGTCCCGCAGGGACGG.GGGG.CAGCCCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGc                 <  2:3774481/101‑1 (MQ=255)
                                                                                    ggggAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGGGCCAGACCGAGGCCGACAGGATCGAGAGCGCGGGGGCAATCAGCAc               >  1:4317317/1‑101 (MQ=25)
                                                                                     gggAGCCCCGCGGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACg              <  1:1077469/101‑1 (MQ=39)
                                                                                      ggAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGAGAGGATCGAGAGCGCGGTGGCAATCAGCACGc             >  2:3791635/1‑101 (MQ=255)
                                                                                       gAGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCt            <  1:362913/101‑1 (MQ=255)
                                                                                        aGTCCCGCAGGGCCGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGGGGCAATCAGCACGCTc           >  1:2727884/1‑101 (MQ=39)
                                                                                        aGTCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTc           <  2:1669702/101‑1 (MQ=255)
                                                                                          tCCCGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCGa         <  1:2944963/101‑1 (MQ=255)
                                                                                           cccGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGGTCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCCGGGGGGCAATCAGCACGCCCGAc        >  2:4453700/1‑101 (MQ=37)
                                                                                           cccGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCGAc        <  1:1490723/101‑1 (MQ=255)
                                                                                           cccGCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGGGGCAATCAGCACGCTCGAc        >  2:3270836/1‑101 (MQ=255)
                                                                                              gCAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGGTCGGGAGGCAAAGCCAGATCGGCAGGGTCGTGAGCGCGGGGGGAAACAGCACGCTCGAcgcc     >  1:4994573/1‑101 (MQ=255)
                                                                                               cAGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCGAcgccc    >  1:4605226/1‑100 (MQ=255)
                                                                                                aGGGACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGGGGCAATAAGCACGCCCGAccccgc   >  1:3414331/1‑101 (MQ=255)
                                                                                                 gggACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCGACgccgcc  <  1:4745885/101‑1 (MQ=255)
                                                                                                 gggACGG.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCGACgccgcc  <  2:2972513/101‑1 (MQ=255)
                                                                                                      |                                                                                                 
TCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAA.GGC.A.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.G.GGGG.CAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCGACGCCGCC  >  NC_014034/1320609‑1320802

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG