Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,138 +G 2.6% coding (20/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,1381.→G 2.6% 13.0 ‑1.3 ‑0.0273coding (20/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (136/130):  total (138/135)
Fisher's exact test strand distribution p-value = 2.78e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.91e-01

Only 100 of 292 total aligned reads displayed.

CCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCG  >  NC_014034/1396038‑1396238
                                                                                                                                                                                                         
  ccccGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCAt                                                                                                    <  1:4939299/101‑1 (MQ=255)
    ccGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCt                                                                                                  <  1:1350155/101‑1 (MQ=255)
        gggggCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCa                                                                                              >  2:2475383/1‑101 (MQ=255)
          gggCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAgg                                                                                            >  2:4479444/1‑101 (MQ=255)
             cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTc                                                                                         <  2:728087/101‑1 (MQ=255)
             cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTc                                                                                         >  2:3674369/1‑101 (MQ=255)
             cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTc                                                                                         >  1:176733/1‑101 (MQ=255)
             cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTc                                                                                         <  1:281683/101‑1 (MQ=255)
             cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTc                                                                                         <  2:2371698/101‑1 (MQ=255)
              gTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTcc                                                                                        >  2:3538117/1‑100 (MQ=255)
               tGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTccc                                                                                       >  1:3710408/1‑99 (MQ=255)
                ggATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTcgcg                                                                                      >  1:3754818/1‑101 (MQ=255)
                ggATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTcgcg                                                                                      >  2:3650320/1‑101 (MQ=255)
                 gATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCgcgc                                                                                     <  1:1998761/101‑1 (MQ=255)
                  aTCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGcgcc                                                                                    <  2:150239/101‑1 (MQ=255)
                  aTCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGcgcc                                                                                    <  2:88173/101‑1 (MQ=255)
                   tCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCgcgcgc                                                                                   >  2:544457/1‑99 (MQ=255)
                    cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGcgccgc                                                                                  >  1:3810150/1‑101 (MQ=255)
                    cGAAACAGGCGCATCTGATGTATATGCCCGGAGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTCGCTCGATACTTTTCCTCATCTAGCAggaagacacccc                                                                                  >  2:2561230/1‑91 (MQ=37)
                     gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCgccgcc                                                                                 >  2:1126209/1‑101 (MQ=255)
                     gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCgccgcc                                                                                 >  2:250188/1‑101 (MQ=255)
                     gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCgccgcc                                                                                 >  1:5048238/1‑101 (MQ=255)
                     gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCgccgcc                                                                                 >  1:1000279/1‑101 (MQ=255)
                     gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCCCgccgcc                                                                                 >  2:1645187/1‑101 (MQ=255)
                      aaaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCgcccccc                                                                                >  1:1708354/1‑100 (MQ=255)
                        aCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACg                                                                              <  1:3548712/101‑1 (MQ=255)
                        aCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACg                                                                              >  1:1492823/1‑101 (MQ=255)
                         cAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCCCGc                                                                             >  1:319337/1‑101 (MQ=255)
                         cAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGc                                                                             >  1:82701/1‑101 (MQ=255)
                         cAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGc                                                                             >  2:412656/1‑101 (MQ=255)
                           ggCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTg                                                                           <  2:3933573/101‑1 (MQ=255)
                            gcgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGc                                                                          <  1:1297400/101‑1 (MQ=255)
                             cgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTggg                                                                         >  2:767283/1‑99 (MQ=255)
                             cgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCg                                                                         <  2:1911183/101‑1 (MQ=255)
                             cgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCg                                                                         <  1:3259368/101‑1 (MQ=255)
                              gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCTCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGt                                                                        >  1:420886/1‑101 (MQ=255)
                              gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCCCGCTGCGt                                                                        >  1:277148/1‑101 (MQ=255)
                              gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGt                                                                        <  2:4414796/101‑1 (MQ=255)
                               cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTc                                                                       <  2:502629/101‑1 (MQ=255)
                               cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTc                                                                       >  1:3977461/1‑101 (MQ=255)
                                  cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCt                                                                    >  2:4791846/1‑101 (MQ=255)
                                  cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCt                                                                    <  2:3380768/101‑1 (MQ=255)
                                       gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTccccc                                                               <  2:1108562/101‑1 (MQ=255)
                                          tatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGGCACGCTGCGTGGCCCCCCCcgc                                                            >  2:838740/1‑101 (MQ=255)
                                           atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCcgcg                                                           >  2:4881231/1‑101 (MQ=255)
                                             gCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCggg                                                         >  2:2714897/1‑101 (MQ=255)
                                              cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCgggg                                                        <  2:3096270/101‑1 (MQ=255)
                                               ccGGTGAGCCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCggggg                                                       <  1:978965/101‑1 (MQ=255)
                                               ccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCggggg                                                       >  2:3719429/1‑101 (MQ=255)
                                                cGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCCCGGGGGc                                                      >  1:3133702/1‑101 (MQ=255)
                                                cGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGAGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGGGTCGCACCCCCCCCGGGGGc                                                      >  1:651189/1‑101 (MQ=38)
                                                 ggTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCg                                                     <  2:3504587/101‑1 (MQ=255)
                                                   tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCggg                                                   >  1:928894/1‑99 (MQ=255)
                                                    gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                  <  1:4299983/101‑1 (MQ=255)
                                                    gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCCCGCTGCGTCGCTCCCCCCCCGGGGGCGGgg                                                  >  1:4461839/1‑101 (MQ=255)
                                                    gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                  <  1:2161864/101‑1 (MQ=255)
                                                    gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                  >  2:4659187/1‑101 (MQ=255)
                                                     agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCCCGGGGGCGTGGa                                                 >  1:3883656/1‑101 (MQ=255)
                                                      gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  2:4857267/101‑1 (MQ=255)
                                                      gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  2:2032241/101‑1 (MQ=255)
                                                       gccgCTTTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTTTGCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCCTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  2:3026100/98‑1 (MQ=38)
                                                       aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  2:637646/101‑1 (MQ=255)
                                                       aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  2:1218781/101‑1 (MQ=255)
                                                         ggCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             <  2:2809797/101‑1 (MQ=255)
                                                         ggCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCGa                                             >  2:4552622/1‑101 (MQ=255)
                                                           cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           <  1:27109/101‑1 (MQ=255)
                                                           cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           >  1:4701924/1‑101 (MQ=255)
                                                           cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           <  2:2956625/101‑1 (MQ=255)
                                                            tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                          <  2:1751270/101‑1 (MQ=255)
                                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCg                                       >  1:3407938/1‑101 (MQ=255)
                                                                 cacccaccGCGGGGGCGTGGCTCGAATATTTTCATCATCAGGCAGGCTAGCGCCGCCACGCTGCGTCGCGCCCCCCGCGGGGGCGTTGATCGAAACCCGGc                                     <  1:2755345/99‑1 (MQ=25)
                                                                  tCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCg                                    <  1:581780/101‑1 (MQ=255)
                                                                  tCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCg                                    >  1:2325966/1‑101 (MQ=255)
                                                                   ccccccGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGa                                   <  1:2674245/101‑1 (MQ=255)
                                                                    cccccGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaa                                  >  2:3217798/1‑101 (MQ=255)
                                                                     ccccGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGGGCGAAACCCGGCGaaa                                 >  1:3850887/1‑101 (MQ=255)
                                                                     ccccGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaa                                 <  1:3680126/101‑1 (MQ=255)
                                                                      cccGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaaa                                <  2:2431218/101‑1 (MQ=255)
                                                                       ccGCGGGGGCGTGGATCGAAACTTTTCGTGATCTGGCAGGCTCGCGCCGCCACGCTGCGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaaaa                               <  2:4157104/101‑1 (MQ=255)
                                                                        cgcgGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAc                              <  2:5047908/101‑1 (MQ=255)
                                                                         gcgGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACg                             >  1:408899/1‑101 (MQ=255)
                                                                            ggggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCAGGGGCGTGGATCGAAACCCGGCGAAAAACGGcc                          <  2:2156287/101‑1 (MQ=255)
                                                                             gggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCa                         <  2:29925/101‑1 (MQ=255)
                                                                              ggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                        >  1:4400940/1‑101 (MQ=255)
                                                                               gCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATg                       <  1:4155837/101‑1 (MQ=255)
                                                                                  tGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCt                    <  1:4424082/101‑1 (MQ=255)
                                                                                   ggATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCCTGCCTc                   >  2:3742562/1‑101 (MQ=255)
                                                                                    gATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTcc                  <  1:4481155/101‑1 (MQ=255)
                                                                                     aTCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCg                 <  1:2992555/101‑1 (MQ=255)
                                                                                      tCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCgg                <  1:754927/101‑1 (MQ=255)
                                                                                       cGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCggg               >  2:4290259/1‑101 (MQ=255)
                                                                                            cTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTca          <  2:1811758/101‑2 (MQ=255)
                                                                                             ttttCGTTATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg         <  1:1113140/101‑1 (MQ=255)
                                                                                             ttttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg         >  1:2506927/1‑101 (MQ=255)
                                                                                              tttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg        >  1:103811/1‑101 (MQ=255)
                                                                                              tttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg        >  2:2092674/1‑101 (MQ=255)
                                                                                              tttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg        >  1:3029040/1‑101 (MQ=255)
                                                                                                tCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCt      >  1:3336901/1‑101 (MQ=255)
                                                                                                 cGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTg     <  1:4659510/101‑1 (MQ=255)
                                                                                                    cATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCTGGCTGTCg  >  2:2301198/1‑101 (MQ=255)
                                                                                                                                                                                                         
CCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCG  >  NC_014034/1396038‑1396238

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG