BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,946	+A	9.1%	coding (828/1578 nt)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,946	1	.→A	9.1%	148.4 ‑3.2 ‑1.2	417	coding (828/1578 nt)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (13/25): ref base (197/182): total (210/207)
Fisher's exact test strand distribution p-value = 4.16e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.12e-02

Only 100 of 448 total aligned reads displayed.


CCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCG  >  NC_014034/1396846‑1397046


   cgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGc                                                                                                           >  1:4157232/1‑101 (MQ=255)


     ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGGCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..t                                                                                                      <  1:4976689/101‑1 (MQ=255)


     ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..t                                                                                                      <  1:4838082/101‑1 (MQ=255)


      gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..Tc                                                                                                     <  1:4956259/101‑1 (MQ=255)


       ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCg                                                                                                    >  2:4972547/1‑101 (MQ=255)


         cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGc                                                                                                  >  1:2307705/1‑101 (MQ=255)


         cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGc                                                                                                  >  2:3322895/1‑101 (MQ=255)


           tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCt.t                                                                                               >  2:958840/1‑101 (MQ=255)


            ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.Tc                                                                                              <  2:1460631/101‑1 (MQ=255)


            ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.Tc                                                                                              >  2:2689207/1‑101 (MQ=255)


             gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.Tcc                                                                                             <  1:646784/101‑1 (MQ=255)


             gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.Tcc                                                                                             <  1:1788990/101‑1 (MQ=255)


                  aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTAGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTg                                                                                       >  1:4520050/1‑101 (MQ=255)


                   aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..c                                                                                    <  1:2135722/101‑1 (MQ=255)


                    aCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..Cg                                                                                   >  2:1096149/1‑101 (MQ=255)


                            agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCt                                                                          <  2:3512569/101‑1 (MQ=255)


                              agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTcc                                                                        <  1:2909133/101‑1 (MQ=255)


                                aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccc                                                                      >  2:1439239/1‑101 (MQ=255)


                                aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTC..CGTG.GGCGCTcccc                                                                      >  2:1226999/1‑101 (MQ=255)


                                 tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCCC.CTG..CGTG.GTCGCTccccc                                                                     >  1:3955416/1‑101 (MQ=255)


                                  agcccGCGCGGACCTCCTCAGACGTCGCCCCCCCCGCGTGGGCGTGGATCGAAACA.GC.CG.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTgccccc                                                                    <  2:225060/98‑1 (MQ=38)


                                  aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                    >  2:3407247/1‑101 (MQ=255)


                                   gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GGCGCTCCCCCcg                                                                   >  2:4440185/1‑101 (MQ=255)


                                       gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                               <  2:1756304/101‑1 (MQ=255)


                                        cgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCgggg                                                              <  1:1988471/101‑1 (MQ=255)


                                            gccctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGTTCCCCCCGCGGGGGCGt                                                          <  1:1854325/99‑1 (MQ=255)


                                            gccctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                          <  2:3633955/99‑1 (MQ=255)


                                            gACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCCCGGGGGCgg                                                          >  2:2315217/1‑100 (MQ=255)


                                              ctcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.G..C.AGT.G..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                         <  1:4689136/91‑1 (MQ=0)


                                               tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.G..C.AGT.G..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                        <  2:409452/92‑1 (MQ=0)


                                               ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                        >  1:3474085/11‑101 (MQ=9)


                                               ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                       <  1:2808515/101‑1 (MQ=255)


                                               attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                       >  2:3242318/11‑101 (MQ=0)


                                                ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                      >  1:4088813/10‑101 (MQ=2)


                                                 tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  1:629030/93‑1 (MQ=2)


                                                 tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                     >  1:1501469/9‑101 (MQ=2)


                                                 tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  1:3217404/93‑1 (MQ=2)


                                                 gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.G..C.AGT.G..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                      >  2:2637875/8‑101 (MQ=2)


                                                 cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  1:936471/101‑1 (MQ=255)


                                                  cccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                       <  1:719953/95‑1 (MQ=2)


                                                    ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  2:4591282/96‑1 (MQ=2)


                                                    cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  2:3157727/101‑1 (MQ=255)


                                                    cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  >  1:3260658/1‑101 (MQ=255)


                                                    acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   >  1:1521890/6‑101 (MQ=9)


                                                     gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  2:4175570/4‑101 (MQ=2)


                                                     aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGGGGGGGTCGaaa                                                 >  1:20549/1‑101 (MQ=255)


                                                     aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 >  2:185027/1‑101 (MQ=255)


                                                       cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                               <  2:4723137/99‑2 (MQ=2)


                                                        cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 >  2:477790/1‑101 (MQ=2)


                                                        cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  1:2838087/101‑1 (MQ=2)


                                                        cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CGAGCGGCGCTCCCCCCGCGGGGCCGTGGATCGaaa                                                 >  2:1677022/1‑101 (MQ=2)


                                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAActc                                              >  1:1057255/1‑99 (MQ=9)


                                                         gTCCCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                             <  1:3648862/101‑4 (MQ=2)


                                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                               <  2:3735872/101‑1 (MQ=2)


                                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                               >  1:4031554/1‑101 (MQ=2)


                                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCa                                            >  1:3577883/1‑101 (MQ=255)


                                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                            <  1:2667094/101‑1 (MQ=255)


                                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCACCGCGGGGGCGTGGATCGAAActaca                                            >  1:4238981/1‑97 (MQ=2)


                                                          tCGCTCCACCCGCGGGGTCGTGGGCCGAAACA.ACGCG.GCGCG.G..G.CCT.A..T.GGCTGTCGCC.CCC..CCCC.GTCGCTCCCCCCCCGGGGGCGTGGATCGAAActgc                                             <  2:710065/101‑4 (MQ=2)


                                                          gcGCGCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                            <  2:624089/100‑1 (MQ=255)


                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                              >  1:1221739/1‑100 (MQ=2)


                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAg                                           >  2:2427233/1‑101 (MQ=255)


                                                           cGCCCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GCCCA.GATC.CGCAA..TCGGC...CGGG.CTC..GATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcctat                                            <  2:2876833/101‑5 (MQ=0)


                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                          <  1:44290/101‑7 (MQ=2)


                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                          >  1:3393917/1‑95 (MQ=2)


                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                           >  1:1927665/1‑96 (MQ=9)


                                                            ccTCCCCCCGCGGGGGCGGGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCacc                                          <  2:4909765/100‑3 (MQ=255)


                                                             cTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCATCt                                         >  2:1133706/1‑101 (MQ=255)


                                                              tCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaa                                           <  1:2944103/101‑2 (MQ=2)


                                                              tCCCCCCGCGGGGGCGTGGATCGAAACA.CC.CG.GCGCC.G..C.AGC.G..AGTGCG.TCTGA.ACA..GGAT.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActatgctgg                                        >  2:2807018/1‑93 (MQ=2)


                                                               ccccccGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCTCCCGCGGGGGCGTGGATCGAAACACCaat                                          >  2:3077635/1‑99 (MQ=2)


                                                               ccccccGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.G..C.AGT.G..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAccccccacc                                        >  2:1299302/1‑93 (MQ=2)


                                                               ccccccGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                        >  2:3037009/1‑93 (MQ=9)


                                                                cccccGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgc                                      >  2:2934152/1‑100 (MQ=255)


                                                                cccccGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                       <  1:1301550/101‑10 (MQ=9)


                                                                cccccGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                       <  2:1579696/101‑10 (MQ=9)


                                                                 ccccGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.C..C.ATG.AGTTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgc                                        <  2:4299223/101‑5 (MQ=0)


                                                                   ccGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcgtcgt                                   >  2:1004960/1‑101 (MQ=255)


                                                                    cgcgGGGGCGTGGAGCGAACCA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                  <  2:799502/101‑1 (MQ=255)


                                                                     gggggggCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CCC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                 <  1:2195171/99‑1 (MQ=255)


                                                                     gcgGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                 >  1:3748730/1‑101 (MQ=255)


                                                                      cgGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                <  1:1394581/101‑1 (MQ=255)


                                                                       gggggCGTGGATCGAAACA.TC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCCCGTCGTTGGt                               >  1:877486/1‑101 (MQ=255)


                                                                       gggggCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                               >  1:4378169/1‑101 (MQ=255)


                                                                        ggggGGTGGATCCAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGTT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                              <  1:4875229/101‑1 (MQ=255)


                                                                        ggggCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                              <  2:3998185/101‑1 (MQ=255)


                                                                        ggggCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAATCAGCTCGTCGTTGGTc                              >  1:3835259/1‑101 (MQ=255)


                                                                             gTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCCTCGGTGGGCAGCCg                         >  2:4335797/1‑101 (MQ=255)


                                                                               ggATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                       >  1:3777720/1‑101 (MQ=255)


                                                                                   cGAAACA.GC.CG.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                   <  2:2069792/101‑1 (MQ=255)


                                                                                    gAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                  >  1:309201/1‑101 (MQ=255)


                                                                                      aaCA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                <  1:1694266/101‑1 (MQ=255)


                                                                                       aCA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc               <  2:196544/101‑1 (MQ=255)


                                                                                       aCA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc               <  1:1439366/101‑1 (MQ=255)


                                                                                        cA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg              <  1:2595785/101‑1 (MQ=255)


                                                                                            c.cT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCg           >  1:2236265/1‑101 (MQ=255)


                                                                                              cT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc          >  2:3896688/1‑101 (MQ=255)


                                                                                                cgcgcG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGGCGTTGGTCAGCCGGACGATGTCCGTCGCt         <  1:2355598/100‑1 (MQ=255)


                                                                                                 gcgcG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc        >  2:1358561/1‑101 (MQ=255)


                                                                                                       g..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc   <  1:2427407/101‑1 (MQ=255)


CCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCG  >  NC_014034/1396846‑1397046

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG