Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,381 +G 17.7% intergenic (‑160/‑798) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,3811.→G17.7% 173.8 ‑0.3 ‑0.7294intergenic (‑160/‑798)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (25/27):  ref base (110/132):  total (135/159)
Fisher's exact test strand distribution p-value = 7.61e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.90e-01

Only 100 of 319 total aligned reads displayed.

ATGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAG  >  NC_014034/1434281‑1434481
                                                                                                     |                                                                                                      
aTGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                                         >  1:3477711/1‑101 (MQ=255)
aTGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                                         <  1:3135559/101‑1 (MQ=255)
aTGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                                         >  1:2512405/1‑101 (MQ=255)
aTGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                                         >  2:456627/1‑101 (MQ=255)
aTGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                                         <  2:2439972/101‑1 (MQ=255)
 tGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..g                                                                                                      <  1:274723/101‑1 (MQ=255)
  ggggTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..Gt                                                                                                     >  2:2074028/1‑101 (MQ=255)
    ggTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..Gtct                                                                                                   >  2:2910432/1‑101 (MQ=255)
      tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                 <  1:1547261/101‑1 (MQ=255)
       atatGCACCCCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCg                                                                                                <  1:354380/101‑1 (MQ=255)
       atatGAACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCg                                                                                                >  2:1022223/1‑101 (MQ=255)
        tatGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGc                                                                                               >  1:2443564/1‑101 (MQ=255)
         atGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGGACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAC..GTCGCCGcc                                                                                              >  2:4755562/1‑100 (MQ=255)
         atGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGct                                                                                              >  1:4088564/1‑101 (MQ=255)
           gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctct                                                                                            >  1:511998/1‑101 (MQ=255)
           gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctct                                                                                            <  2:3610759/101‑1 (MQ=255)
            cACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTg                                                                                           <  1:743815/101‑1 (MQ=255)
              ccGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCt                                                                                         <  2:1615412/101‑1 (MQ=255)
               cGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCtt                                                                                        <  1:4557909/101‑1 (MQ=255)
                gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTc                                                                                       <  1:1217951/101‑1 (MQ=255)
                gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTc                                                                                       <  1:1410849/101‑1 (MQ=255)
                   tACCGTGGATCGAATCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg                                                                                    <  2:3349156/101‑1 (MQ=255)
                   tACCGTGGATCGAACCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg                                                                                    <  1:4948242/101‑1 (MQ=255)
                   tACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg                                                                                    >  2:2821522/1‑101 (MQ=255)
                    aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg.g                                                                                  <  1:424810/101‑1 (MQ=255)
                      cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCt                                                                                <  1:29572/101‑1 (MQ=255)
                       gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTg                                                                               >  1:3942193/1‑101 (MQ=255)
                        gggATCGAAACAGGCTGCTGTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGc                                                                              <  2:4701885/100‑1 (MQ=255)
                         ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCg                                                                             >  2:70582/1‑101 (MQ=255)
                          gATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCgc                                                                            >  1:1005532/1‑100 (MQ=255)
                             cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCaa                                                                         >  1:1120665/1‑101 (MQ=255)
                             cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCaa                                                                         >  2:1188386/1‑101 (MQ=255)
                             cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCaa                                                                         >  1:3720361/1‑101 (MQ=255)
                             cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCaa                                                                         <  1:1474998/101‑1 (MQ=255)
                                 aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                     <  2:3273571/101‑1 (MQ=255)
                                   aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGt                                                                   >  1:2009036/1‑101 (MQ=255)
                                    ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTc                                                                  <  1:4990879/101‑1 (MQ=255)
                                     gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCg                                                                 <  1:3211972/101‑1 (MQ=255)
                                        gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTc                                                              >  2:1547354/1‑101 (MQ=255)
                                        gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTc                                                              >  2:4240721/1‑101 (MQ=255)
                                        gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTc                                                              >  1:4748936/1‑101 (MQ=255)
                                        gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTc                                                              >  2:3142015/1‑101 (MQ=255)
                                           ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTcccc                                                           <  2:1340790/101‑1 (MQ=255)
                                            tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTccccc                                                          <  2:2725288/101‑1 (MQ=255)
                                                 ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCgg                                                     >  2:2992414/1‑101 (MQ=255)
                                                  gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCggg                                                    <  2:3673680/101‑1 (MQ=255)
                                                  gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCggg                                                    >  1:1848293/1‑101 (MQ=255)
                                                    acacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GGTGCGTCAACCTTTTCGCTCCCCCCCCggggg                                                  >  1:4919064/1‑101 (MQ=255)
                                                     cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGc                                                 <  1:2137651/101‑1 (MQ=255)
                                                      acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCCCGGGGGCg                                                >  2:3743507/1‑101 (MQ=255)
                                                       ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGt                                               <  2:2008892/101‑1 (MQ=255)
                                                        cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTg                                              <  1:4456179/101‑1 (MQ=255)
                                                         tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTgg                                             <  2:3893103/101‑1 (MQ=255)
                                                          gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAATC..GTCTCCGCGCTGCTTCACG.GCTGAGTCAACCTTTTCGCTCCCCCCGCGGCGTCGTGGa                                            <  2:134567/101‑1 (MQ=37)
                                                          gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGa                                            <  1:4195616/101‑1 (MQ=255)
                                                            cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTgg                                             >  2:2612161/10‑101 (MQ=2)
                                                               gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  1:666075/95‑1 (MQ=2)
                                                               gctatcgTCGCTCCCCCCGCGGGGGCCTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  1:3038358/95‑1 (MQ=2)
                                                                gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  2:2480365/101‑1 (MQ=255)
                                                                ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  1:2530530/6‑101 (MQ=2)
                                                                 tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        <  1:2184402/97‑1 (MQ=2)
                                                                  tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                    <  1:3625375/101‑1 (MQ=255)
                                                                  atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       >  1:3587541/4‑101 (MQ=2)
                                                                  atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGAGGGCGTGGATCGaa                                       >  1:4357137/4‑101 (MQ=2)
                                                                   atctCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:4504810/98‑1 (MQ=2)
                                                                    ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                  <  2:931222/101‑1 (MQ=255)
                                                                    ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                  <  1:2599153/101‑1 (MQ=255)
                                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                 <  1:2416532/101‑1 (MQ=255)
                                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                  <  1:2306976/101‑4 (MQ=2)
                                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                               <  2:4006916/101‑1 (MQ=255)
                                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                              <  2:2572207/101‑1 (MQ=255)
                                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                              >  2:4286471/1‑101 (MQ=255)
                                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                              >  2:1678185/1‑101 (MQ=255)
                                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAacgctgc                                >  2:3838787/1‑96 (MQ=2)
                                                                         cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                             <  2:4399044/101‑1 (MQ=255)
                                                                          tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGGTCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgccggg                               >  2:3436324/1‑95 (MQ=2)
                                                                          tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                               >  2:4178257/1‑95 (MQ=2)
                                                                          tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                               >  1:3841247/1‑95 (MQ=2)
                                                                          cccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                               <  2:2344804/100‑7 (MQ=2)
                                                                           ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                              >  2:555257/1‑94 (MQ=2)
                                                                           ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                              <  2:2141601/101‑8 (MQ=2)
                                                                            cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGACTGCAt                          >  2:2247476/1‑101 (MQ=255)
                                                                             ccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                         <  1:1797492/101‑1 (MQ=255)
                                                                              cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                           <  2:3009559/101‑11 (MQ=0)
                                                                              cccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                        <  1:2910645/101‑1 (MQ=255)
                                                                                  cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                    >  2:268748/1‑101 (MQ=255)
                                                                                   gggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                   >  1:4891461/1‑101 (MQ=255)
                                                                                       gCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAggg               >  1:947761/1‑101 (MQ=255)
                                                                                        cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa              >  1:1702761/1‑101 (MQ=255)
                                                                                        cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa              <  2:4342333/101‑1 (MQ=255)
                                                                                         gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc             <  1:2723835/101‑1 (MQ=255)
                                                                                         gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc             <  1:3295821/101‑1 (MQ=255)
                                                                                          tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa            <  2:298166/101‑1 (MQ=255)
                                                                                           ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg           >  1:4015336/1‑101 (MQ=255)
                                                                                            gATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAgg          <  2:851408/101‑1 (MQ=255)
                                                                                             aTCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGc         >  2:823568/1‑101 (MQ=255)
                                                                                                gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt      <  2:4532142/101‑1 (MQ=255)
                                                                                                gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt      <  2:3654491/101‑1 (MQ=255)
                                                                                                 aaaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg     <  2:4088261/101‑1 (MQ=255)
                                                                                                    c..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg  >  1:1340938/1‑101 (MQ=255)
                                                                                                     |                                                                                                      
ATGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAG  >  NC_014034/1434281‑1434481

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG