Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,732,696 +C 100% intergenic (‑120/‑283) RCAP_rcc01601 ← / → tgt FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,732,6961.→C100.0% 15.7 72intergenic (‑120/‑283)RCAP_rcc01601/tgtFeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (7/58):  ref base (4/3):  total (11/61)
Fisher's exact test strand distribution p-value = 8.66e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.27e-02

Only 100 of 101 total aligned reads displayed.

TAGAGTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.CC...GGGC..GGGCGG..G..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTCC  >  NC_014034/1732597‑1732784
                                                                                                     |                                                                                                  
tAGAGTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.c                                                                                                   <  1:2781130/101‑1 (MQ=255)
tAGAGTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.c                                                                                                   <  1:1504584/101‑1 (MQ=255)
 agagTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGAAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.cc                                                                                                  >  1:1643396/1‑101 (MQ=255)
  gagTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.ccc                                                                                                 >  1:4476506/1‑100 (MQ=255)
   agTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.cccg                                                                                                >  2:3786240/1‑99 (MQ=255)
   agTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.cccg                                                                                                <  1:2435477/101‑3 (MQ=255)
    gTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.ccccg                                                                                               >  2:2127875/1‑98 (MQ=255)
    gTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.Cc...cgg                                                                                            <  1:2056753/101‑1 (MQ=255)
    gTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.Cc...cgg                                                                                            >  2:2293830/1‑101 (MQ=255)
     tttATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.Cc...cggg                                                                                           <  2:3511691/101‑2 (MQ=255)
     tttATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.Cc...cggg                                                                                           >  2:1399159/1‑100 (MQ=255)
      ttATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.Cc...cgggc                                                                                          <  1:2575501/101‑3 (MQ=255)
       tatataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..g                                                                                        >  2:2310360/1‑101 (MQ=255)
       tatataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.Cc...cgggcc                                                                                         >  1:1375446/1‑98 (MQ=255)
       tatataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.Cc...cgggag                                                                                         >  2:4159158/1‑98 (MQ=255)
        atataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCGGC.Cc...gggc..gg                                                                                       >  2:4490965/1‑101 (MQ=255)
        atataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.ccccggcgg                                                                                           >  1:2424495/1‑94 (MQ=255)
        atataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.Cc...cggg..ggg                                                                                      >  2:1567708/1‑101 (MQ=255)
         tataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGGGGGGCCGCGGC.GA.ccccggccgg                                                                                          >  2:3776304/1‑93 (MQ=255)
         tataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGGCGCGGG.GAGCc...ggggcgg                                                                                        >  2:1063263/1‑97 (MQ=255)
          ataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..gggg                                                                                     <  1:3930975/101‑2 (MQ=255)
           taGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGGGGG.GA.Cc...cggg..gggggg                                                                                   >  2:2893054/1‑101 (MQ=255)
            aGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGGCGCGGC.GG.CC...CGG...GGGCgg..g..g                                                                             >  1:4382640/1‑101 (MQ=255)
             gggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..gggggct                                                                                  <  2:1819975/101‑5 (MQ=255)
              ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GG.CC...CCCC..GGGGgg..g..gg                                                                            >  1:1904076/1‑100 (MQ=255)
              ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..gggggctc                                                                                 <  1:3803435/101‑6 (MQ=255)
               gTATCGGGGGGAGGGGTAAAGGCCGGCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..gggggctcc                                                                                <  1:1072471/101‑7 (MQ=255)
               gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGG.NA.Cc...cggg..ggnngnnnnn                                                                               >  2:2127742/1‑96 (MQ=255)
               gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..gggggctcc                                                                                <  2:597974/101‑7 (MQ=255)
               gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGG..CGGCgg..g..gg                                                                            >  1:2230135/1‑100 (MQ=255)
               gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGC..GCGGgg..g..gg                                                                            >  2:3702518/1‑100 (MQ=255)
               gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.CC...CGCC..GCGCcg..g..gtc                                                                           >  2:4882319/1‑99 (MQ=255)
                tATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..gggggctccg                                                                               <  2:28758/101‑8 (MQ=39)
                 aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GG.CC...CGGG..GCGGGG..G..Gcccc                                                                         >  1:1329482/1‑101 (MQ=255)
                   cGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCA.GGGCGG..G..Gcccc                                                                         >  2:1286185/1‑101 (MQ=255)
                   cGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGC..GGGGGC..G..Gcgccc                                                                        >  2:616588/1‑101 (MQ=255)
                    ggggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGGGGC.CA.CC...CGGG..GGGGgg..g..ggggcccc                                                                      >  1:1680076/1‑94 (MQ=255)
                     gggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..gggggg..gcgcgcccc                                                                        >  2:3999604/1‑93 (MQ=255)
                     gggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGC..GGGGGG..G..Ggccgccc                                                                      >  1:4114447/1‑97 (MQ=255)
                      ggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGC..CGGGGG..G..Gcgcccccc                                                                     >  2:1606560/1‑98 (MQ=255)
                       gggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGC..GGGGGG..T..Cccccccccc                                                                    >  2:744264/1‑97 (MQ=255)
                         gagaGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGG..GGGGGG..G..GGCCGCCCAccc                                                                  >  2:4377324/1‑101 (MQ=255)
                          agagGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.CC...CGGG..GGGGGG..G..GGCCCCCCCccccc                                                                >  1:2287093/1‑101 (MQ=255)
                              gTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCg                                                               <  1:2128023/101‑1 (MQ=25)
                               tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGG..CGGGGG..G..GCGGCCGCCccccccccc                                                            >  1:1389059/1‑97 (MQ=255)
                               tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCgg                                                              <  2:3261251/101‑1 (MQ=25)
                               tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCgg                                                              <  2:1307984/101‑1 (MQ=25)
                                aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGc                                                             <  2:1701662/101‑1 (MQ=25)
                                  ccgatctCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGt                                                           <  1:3391033/95‑1 (MQ=21)
                                  aGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCCCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGt                                                           <  2:1388472/101‑1 (MQ=25)
                                  aGGCCGTCGGTGCGGGGCGGAAAGCCGCGTCCCAAGCAGGGTTTCGGCGCGGCGGGGCCGCGGC.GACCC...GGGCCGGGGCTC..C..GCCCCCGCACCCCCGGCGt                                                           <  2:4172312/101‑1 (MQ=25)
                                   ggCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTa                                                          <  1:1317713/101‑1 (MQ=25)
                                    gCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.t                                                        <  2:4494322/101‑1 (MQ=25)
                                    gCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.t                                                        <  1:3702860/101‑1 (MQ=25)
                                    gCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.t                                                        <  2:4382323/101‑1 (MQ=25)
                                     ccGGCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.tt                                                       <  2:2110618/101‑1 (MQ=21)
                                      cGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.ttt                                                      <  2:2340733/101‑1 (MQ=25)
                                       gTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTg                                                     <  2:1488043/101‑1 (MQ=21)
                                            tGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCa                                                <  2:525119/101‑1 (MQ=21)
                                            tGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCa                                                <  1:4094346/101‑1 (MQ=21)
                                              cGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCaag                                              <  1:1766103/101‑1 (MQ=21)
                                               ggggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCaaga                                             <  1:2498349/101‑1 (MQ=21)
                                               ggggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCaaga                                             <  2:2583877/101‑1 (MQ=21)
                                                 ggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCGCCCCCGGCGTA.TTTGCGCCaagaag                                           <  1:3395357/101‑1 (MQ=18)
                                                 ggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCaagaag                                           <  2:1643264/101‑1 (MQ=18)
                                                 ggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCaagaag                                           <  1:3776589/101‑1 (MQ=18)
                                                 ggCGGAAAGCCGCGTGCCAAGCAGGGTTGCGCCACGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCaagaag                                           <  1:4377641/101‑1 (MQ=18)
                                                  gCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAagaaga                                          <  2:4739715/101‑1 (MQ=18)
                                                   cGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAgaagaa                                         <  2:3221040/101‑1 (MQ=18)
                                                    ggAAAGCCGCGTGCAAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCGCGGCGTA.TTTGCGCCAAGAAGaaa                                        <  2:2504440/101‑1 (MQ=18)
                                                      aaaGCCGCGTGCGAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACa                                      <  1:4375377/101‑1 (MQ=18)
                                                       aaGCGGCGTGCCAGGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACaa                                     <  1:3447389/101‑1 (MQ=17)
                                                       aaGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGC..GGGGGG..GCCGCGCCCGCACCCCCCGCGCA.TTTTTGACAAGAAGAAAAaa                                     >  2:3089241/1‑101 (MQ=255)
                                                         gCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGc                                   <  2:4592591/101‑1 (MQ=18)
                                                         gCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGc                                   <  1:4920762/101‑1 (MQ=18)
                                                            gcgTGGCAAGCAGGGTTTCGCGGGGGCGGGGCGGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCggg                                <  1:1029493/101‑1 (MQ=17)
                                                            gcgTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCTGCGTA.TTTGCGCCAAGAAGAAACAAGCggg                                <  1:4491285/101‑1 (MQ=18)
                                                              gggCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGAc                              <  2:83904/99‑1 (MQ=18)
                                                              gTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.CCCGGGGGC..GGGGGG..G..CCCCCCCCACCCCCCGCGCATTTTTCGCC.AGAAAAAACaaaagggga                              >  2:2322397/1‑94 (MQ=255)
                                                               tGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGAcc                             <  1:3831048/101‑1 (MQ=18)
                                                               tGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGAcc                             <  1:65293/101‑1 (MQ=18)
                                                               tGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGAcc                             <  2:3229039/101‑1 (MQ=18)
                                                                gCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCa                            <  2:4697739/101‑1 (MQ=18)
                                                                 ccAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAg                           <  2:1042902/101‑1 (MQ=18)
                                                                 ccAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAg                           <  2:4924579/101‑1 (MQ=18)
                                                                  cAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAgg                          <  2:4750756/101‑1 (MQ=18)
                                                                    aGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGcc                        <  1:1477665/101‑1 (MQ=18)
                                                                      cAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccc                      <  2:2592945/101‑1 (MQ=18)
                                                                      cAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccc                      <  2:3097491/101‑1 (MQ=18)
                                                                      cAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccc                      <  1:1411749/101‑1 (MQ=18)
                                                                            ttCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCa                <  1:75397/101‑1 (MQ=18)
                                                                             tCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAg               <  1:114457/101‑1 (MQ=18)
                                                                             tCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAg               <  1:1762962/101‑1 (MQ=18)
                                                                              cgccgcGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGc              <  1:257664/101‑1 (MQ=18)
                                                                                   cggcggGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCAt         <  2:462330/101‑1 (MQ=18)
                                                                                     ggggggCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGc       <  1:4261380/99‑1 (MQ=18)
                                                                                     gcggGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGc       <  1:1327336/101‑1 (MQ=18)
                                                                                     gcggGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGc       <  2:254259/101‑1 (MQ=18)
                                                                                         ggCCGCGGC.GACCC...GGGC..GGGGGGTCC..CCCCCCCCCCCCCCCGCGGA.TTTTCGCCCAGAAGAAAACAACGGGACCAGGCCCCCCCGCAGCCCCACGCCCTc   >  2:871571/1‑101 (MQ=255)
                                                                                          gCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTcc  <  2:2286929/101‑1 (MQ=18)
                                                                                                     |                                                                                                  
TAGAGTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.CC...GGGC..GGGCGG..G..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTCC  >  NC_014034/1732597‑1732784

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG