Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,769,370 +G 100% coding (442/744 nt) RCAP_rcc01636 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,769,3701.→G100.0% 38.8 157coding (442/744 nt)RCAP_rcc01636hypothetical protein
Reads supporting (aligned to +/- strand):  new base (66/77):  ref base (1/13):  total (67/90)
Fisher's exact test strand distribution p-value = 4.17e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.53e-01

Only 100 of 173 total aligned reads displayed.

GTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.C.GG..C.A.GGGGCGGTGGTC.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769273‑1769462
                                                                                                        |                                                                                              
 tgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTcgc                                                                                               <  2:2355577/101‑3 (MQ=255)
  gCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTcgcg                                                                                              >  2:359160/1‑98 (MQ=255)
     gCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTcgcgcgc                                                                                           <  2:4030080/101‑7 (MQ=255)
       aGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTcgcgcgcgg                                                                                         >  1:1097309/1‑93 (MQ=255)
         cGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTcgcgcgcgggc                                                                                       <  1:104985/101‑11 (MQ=255)
         cGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTcgcgcgcgggc                                                                                       <  2:3391019/101‑11 (MQ=255)
         cGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTcgcgcgcgggc                                                                                       <  1:1762464/101‑11 (MQ=255)
           tCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCtt                                                                                   <  1:780498/101‑1 (MQ=255)
            cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCttt                                                                                  <  1:1460026/101‑1 (MQ=255)
            cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCttt                                                                                  <  1:1768442/101‑1 (MQ=255)
             ggCGATCAGGGCCAACTGGATGCGGCCCGCGCGGCGGAAATCGGCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTg                                                                                 <  1:2333763/101‑1 (MQ=255)
               cGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGcc                                                                               <  2:1928445/101‑1 (MQ=255)
                gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCt                                                                              >  2:1335792/1‑101 (MQ=255)
                 aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTC.GC.GCGCGGGCTTTGCCtt                                                                             <  2:322073/101‑1 (MQ=255)
                 aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGGGGTCGCG.CG.CGGGCTTTGCCtt                                                                             >  1:4006574/1‑101 (MQ=255)
                  tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTg                                                                            >  2:2111750/1‑101 (MQ=255)
                  tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTg                                                                            >  1:653363/1‑101 (MQ=255)
                  tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.C.GGGCC.G.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTg                                                                            >  2:2376134/1‑101 (MQ=255)
                   cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTgg                                                                           >  1:1319144/1‑101 (MQ=255)
                   cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTgg                                                                           >  1:636636/1‑101 (MQ=255)
                     ggACCAACTGGAGGCGGCCCGCGCGGGGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTC.GCGCG.CGGGCTTTGCCTTgggg                                                                         <  1:277513/101‑1 (MQ=255)
                      gACCCACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        <  1:1417919/101‑1 (MQ=255)
                      gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        <  1:1203649/101‑1 (MQ=255)
                      gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        <  1:3015333/101‑1 (MQ=255)
                       gccAACTGGATGCGGCCCGCGCGGGGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTc                                                                       <  1:3559072/100‑1 (MQ=255)
                        ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                                      <  1:2586449/101‑1 (MQ=255)
                         cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGa                                                                     <  1:2756878/101‑1 (MQ=255)
                          aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAg                                                                    <  2:4674938/101‑1 (MQ=255)
                           aCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAgg                                                                   >  2:3754436/1‑101 (MQ=255)
                            cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgc                                                                  >  2:1675379/1‑101 (MQ=255)
                                gtGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              <  2:4444096/100‑1 (MQ=255)
                                aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              >  1:5007545/1‑101 (MQ=255)
                                aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              >  1:1193348/1‑101 (MQ=255)
                                aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              <  1:607664/101‑1 (MQ=255)
                                 tGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTc                                                             >  1:3407897/1‑101 (MQ=255)
                                 tGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTc                                                             <  1:1630132/101‑1 (MQ=255)
                                   cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           >  2:2564601/1‑101 (MQ=255)
                                   cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           <  1:4522495/101‑1 (MQ=255)
                                    ggCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCgg                                                          <  2:3334885/101‑1 (MQ=255)
                                      cccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGc                                                        <  1:1877363/101‑1 (MQ=255)
                                       ccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGcc                                                       >  2:3543058/1‑100 (MQ=255)
                                         tcggggcggAAATCGTCGGGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                                     <  1:1776938/97‑1 (MQ=255)
                                            cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:1211153/101‑1 (MQ=255)
                                            cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:501546/101‑1 (MQ=255)
                                            cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGGGGTCGCG.CG.CGGGCTTTGCCTTGGGGTGGAGGCGCTTCCGGGCACCGAt                                                  >  1:4708803/1‑101 (MQ=255)
                                             ggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATg                                                 <  2:2063135/101‑1 (MQ=255)
                                              gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                                <  1:1032745/101‑1 (MQ=255)
                                              gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                                >  1:2368187/1‑101 (MQ=255)
                                               gggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcg                                               <  2:74469/100‑1 (MQ=255)
                                                ggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcgc                                              >  2:842549/1‑101 (MQ=255)
                                                 gAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             >  2:757854/1‑101 (MQ=255)
                                                  aaaTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                                            >  2:4953075/1‑101 (MQ=255)
                                                  aaaTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                                            <  2:2870339/101‑1 (MQ=255)
                                                  aaaTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                                            >  1:3829180/1‑101 (MQ=255)
                                                    aTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCt                                          >  2:4143604/1‑101 (MQ=255)
                                                      cgtcgCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        >  2:56495/1‑101 (MQ=255)
                                                       gtcgCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCCCCGATGCGCTCCTTGa                                       >  1:3670666/1‑101 (MQ=255)
                                                       gtcgCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGa                                       <  2:18660/101‑1 (MQ=255)
                                                       gtcgCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGa                                       <  2:2512628/101‑1 (MQ=255)
                                                        tcgCGGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAt                                      <  2:3469520/101‑1 (MQ=255)
                                                        gcgcgGCTGTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGGCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAt                                      <  2:69471/100‑1 (MQ=255)
                                                          gcgGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    <  2:1284174/101‑1 (MQ=255)
                                                          gcgGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    <  1:869663/101‑1 (MQ=255)
                                                          gcgGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    <  1:914395/101‑1 (MQ=255)
                                                          gcgGCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    <  2:1541382/101‑1 (MQ=255)
                                                            ggCTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  >  2:356768/1‑101 (MQ=255)
                                                              cTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtc                                <  2:3047425/101‑1 (MQ=255)
                                                              cTTTGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtc                                >  2:2027555/1‑101 (MQ=255)
                                                                ttGGGCGCGGTCGATGTGG.GG..CGG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCCCCGATGCGCTCCTTGATTTCGTcgc                              <  2:367405/101‑1 (MQ=255)
                                                                ttGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgc                              >  2:2555009/1‑101 (MQ=255)
                                                                 tGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             <  1:2755377/101‑1 (MQ=255)
                                                                 tGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             <  1:3469196/101‑1 (MQ=255)
                                                                 tGGGCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             <  1:4883823/101‑1 (MQ=255)
                                                                  atctgcgGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccg                            <  2:2287400/97‑1 (MQ=255)
                                                                   ggCGCGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccgc                           <  1:359213/101‑1 (MQ=255)
                                                                    gcgcGGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCcgcg                          <  1:1436051/101‑1 (MQ=255)
                                                                      gcgGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccgagat                        >  1:2287564/1‑97 (MQ=255)
                                                                       cgGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       <  1:2086193/101‑1 (MQ=255)
                                                                       cgGTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       <  1:1675508/101‑1 (MQ=255)
                                                                         gTCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                     >  2:11418/1‑101 (MQ=255)
                                                                          tCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                    >  2:3073546/1‑101 (MQ=255)
                                                                          tCGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                    >  2:4917400/1‑101 (MQ=255)
                                                                           cGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   >  1:3107249/1‑101 (MQ=255)
                                                                           cGATGT.CGGG..C.AGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   >  2:2100404/1‑101 (MQ=255)
                                                                                    tcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTg              <  2:1130941/93‑1 (MQ=255)
                                                                                     tccgatctgggCGGTTGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCTCCGCGCATCGCGGGCTgg             <  1:4651481/93‑1 (MQ=255)
                                                                                     cgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTgg             >  2:4491051/8‑101 (MQ=255)
                                                                                      gggcggggggCGTTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            <  2:2192936/95‑1 (MQ=255)
                                                                                      gggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            >  2:1039176/7‑101 (MQ=255)
                                                                                      gggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            >  2:3367948/7‑101 (MQ=255)
                                                                                      gggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            >  2:3111348/7‑101 (MQ=255)
                                                                                        gcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc          <  2:4787926/97‑1 (MQ=255)
                                                                                         cagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         <  2:4578860/98‑1 (MQ=255)
                                                                                           gggggCGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccga       >  1:98745/2‑101 (MQ=255)
                                                                                            ggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  2:862467/101‑1 (MQ=255)
                                                                                             gggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTggagatagga     >  2:4651158/1‑93 (MQ=255)
                                                                                              ggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgc    <  1:1943878/101‑1 (MQ=255)
                                                                                              ggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgc    <  1:2252207/101‑1 (MQ=255)
                                                                                               gCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgcc   <  2:3872928/101‑1 (MQ=255)
                                                                                                cGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgccg  <  1:2808367/101‑1 (MQ=255)
                                                                                                        |                                                                                              
GTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.C.GG..C.A.GGGGCGGTGGTC.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769273‑1769462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG