Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,948,873 +G 100% coding (1047/2472 nt) quiP ← acyl‑homoserine‑lactone acylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,948,8731.→G100.0% 804.6 210coding (1047/2472 nt)quiPacyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand):  new base (103/107):  ref base (0/0):  total (103/107)

Only 100 of 229 total aligned reads displayed.

TTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.G.CCC.ACCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTGCGCC  >  NC_014034/1948775‑1948967
                                                                                                       |                                                                                              
ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTa                                                                                                 >  2:3307512/1‑101 (MQ=255)
ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTa                                                                                                 <  2:2175990/101‑1 (MQ=255)
ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTa                                                                                                 <  2:1582993/101‑1 (MQ=255)
   tCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTagcg                                                                                              <  1:3694649/101‑4 (MQ=255)
    cGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTagcgc                                                                                             >  2:382379/1‑97 (MQ=255)
    cGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTagcgc                                                                                             >  1:308470/1‑97 (MQ=255)
     ggTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTagcgct                                                                                            <  1:1796178/101‑6 (MQ=255)
      gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTc                                                                                           >  2:2610776/1‑101 (MQ=255)
       tCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCg                                                                                          <  2:652102/101‑1 (MQ=255)
       gccAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCg                                                                                          <  1:1000489/100‑1 (MQ=255)
         cAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGTGCTGCGGTAGCGCTTggg                                                                                        >  2:3370782/1‑101 (MQ=255)
         cAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCggg                                                                                        >  2:2957573/1‑101 (MQ=255)
            cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCCCgggtcg                                                                                     <  2:3000241/101‑4 (MQ=255)
             gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                                                    <  1:1266678/101‑1 (MQ=255)
              cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGttt                                                                                   >  1:705431/1‑101 (MQ=255)
               agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATTACCTTGCGGGTGGTGAACT.CGG.CCCAACCTTCGGGGATGCGGTAGCGCTCGGGCGtatt                                                                                  >  2:833165/1‑101 (MQ=255)
               agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                                                  >  1:2998002/1‑101 (MQ=255)
                gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                                                 <  1:1560129/101‑1 (MQ=255)
                gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                                                 <  1:3594944/101‑1 (MQ=255)
                gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                                                 <  2:2790958/101‑1 (MQ=255)
                  gtgagggtgaTCGGCGCGGCACCCTTGACCGTGACGTTCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCgg                                                                               <  1:1255271/101‑1 (MQ=255)
                    gagggtgaCCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGCACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTCTCGGAt                                                                             >  2:4150399/1‑101 (MQ=255)
                    gagggggaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGGGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGTGTTTTCgggt                                                                             >  1:2498736/1‑99 (MQ=255)
                     agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                                            >  2:1813348/1‑101 (MQ=255)
                     agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                                            >  1:2377867/1‑101 (MQ=255)
                       ggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCaa                                                                          >  2:505754/1‑101 (MQ=255)
                        gtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAg                                                                         <  1:294905/101‑1 (MQ=255)
                        gtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAg                                                                         >  1:1448359/1‑101 (MQ=255)
                          gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                                                       >  2:4591143/1‑101 (MQ=255)
                          gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                                                       <  2:1195278/101‑1 (MQ=255)
                            tCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTcc                                                                     <  2:4688081/101‑1 (MQ=255)
                             cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                                                    >  1:1352613/1‑101 (MQ=255)
                              ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                                                   <  2:2757845/101‑1 (MQ=255)
                              ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                                                   <  1:499114/101‑1 (MQ=255)
                              ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                                                   <  1:4166650/101‑1 (MQ=255)
                              ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                                                   <  1:103039/101‑1 (MQ=255)
                              ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAACTTCCTc                                                                   >  2:4210361/1‑101 (MQ=255)
                                tctggCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcga                                                                 <  2:754933/98‑1 (MQ=255)
                                 gcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgac                                                                >  2:1527873/1‑101 (MQ=255)
                                 gcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgac                                                                <  2:1641021/101‑1 (MQ=255)
                                  cgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                                               <  2:3516432/101‑1 (MQ=255)
                                    gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                                             >  2:1311110/1‑101 (MQ=255)
                                    gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                                             <  2:4395037/101‑1 (MQ=255)
                                     cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                                            <  2:2498562/101‑1 (MQ=255)
                                      aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                                           >  1:4692071/1‑101 (MQ=255)
                                         cTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                                        <  1:4914102/101‑1 (MQ=255)
                                         cTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                                        >  2:1095569/1‑101 (MQ=255)
                                                gTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                                                 <  1:2537965/101‑1 (MQ=255)
                                                gTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                                                 <  1:3371045/101‑1 (MQ=255)
                                                gTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                                                 >  1:3422434/1‑101 (MQ=255)
                                                 tGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                                                <  2:1721619/101‑1 (MQ=255)
                                                 tGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                                                <  1:1698944/101‑1 (MQ=255)
                                                 tGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                                                >  1:3255019/1‑101 (MQ=255)
                                                 tGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                                                >  1:514493/1‑101 (MQ=255)
                                                  gACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                                               <  1:1043209/101‑1 (MQ=255)
                                                   aCGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGGTCCTCCACGACCATGTCCTCGTCATc                                              >  2:3893849/1‑101 (MQ=255)
                                                    cGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                                             >  2:2760901/1‑101 (MQ=255)
                                                    cGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                                             <  1:1484395/101‑1 (MQ=255)
                                                     gATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                                            <  2:1543943/101‑1 (MQ=255)
                                                     gATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                                            >  1:3910217/1‑101 (MQ=255)
                                                     gATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                                            >  2:2900795/1‑101 (MQ=255)
                                                      aTCACCTTGCGGGTGGTGAACG.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGc                                           <  2:5012769/101‑1 (MQ=255)
                                                        cACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                                         >  2:2419746/1‑101 (MQ=255)
                                                          ccTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAg                                       <  2:329719/101‑1 (MQ=255)
                                                           cTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAgg                                      >  1:108498/1‑101 (MQ=255)
                                                           cTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAgg                                      >  1:2434807/1‑101 (MQ=255)
                                                           cTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAgg                                      <  1:744126/101‑1 (MQ=255)
                                                            ttGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggc                                     >  1:946611/1‑101 (MQ=255)
                                                              gCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg                                   >  1:320604/1‑101 (MQ=255)
                                                                  gtggtgAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGCCGAACAGGTCCCCGCAACCGGCGAAGGgggcggg                               >  2:2713247/1‑100 (MQ=255)
                                                                   tggtgAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTg                              >  2:1782651/1‑101 (MQ=255)
                                                                    ggtgAACTAG.G.CCCAACCCTCGGGGCTGCGGTATCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAATGCGGCGGTGa                             <  1:4365250/101‑1 (MQ=255)
                                                                    ggtgAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa                             <  2:3197622/101‑1 (MQ=255)
                                                                    ggtgAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa                             >  1:2456969/1‑101 (MQ=255)
                                                                     gtgAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGAt                            >  2:267023/1‑101 (MQ=255)
                                                                     gtgAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGAt                            <  1:3882153/101‑1 (MQ=255)
                                                                      tgAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATg                           <  2:3334054/101‑1 (MQ=255)
                                                                      tgAACT.C.GTCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATg                           <  1:483249/101‑1 (MQ=255)
                                                                       gAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc                          >  1:3479222/1‑101 (MQ=255)
                                                                       gAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGGGATGc                          >  2:4297182/1‑101 (MQ=255)
                                                                             tcggcccaaCCCTCGGGGCTGTGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCa                      >  1:2862128/9‑101 (MQ=255)
                                                                             tcggcccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCa                      <  1:140298/93‑1 (MQ=255)
                                                                              cggcccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAg                     >  2:2339950/8‑101 (MQ=255)
                                                                               ggcccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGc                    >  2:4017889/7‑101 (MQ=255)
                                                                               ggcccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGGGATGCCCCAGc                    >  2:2280895/7‑101 (MQ=255)
                                                                                gcccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGcc                   <  1:992240/96‑1 (MQ=255)
                                                                                gcccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCGCGACGAACAGGGCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGcc                   <  1:4210678/96‑1 (MQ=255)
                                                                                 cccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCa                  <  2:200798/97‑1 (MQ=255)
                                                                                 cccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGCCGGCGGGGATGCCCCAGcga                  >  1:793829/5‑99 (MQ=255)
                                                                                   c.ACCCGCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGc               <  2:322812/101‑1 (MQ=255)
                                                                                     aCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCt              >  2:1364040/1‑101 (MQ=255)
                                                                                       ccTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTgg            >  1:2747902/1‑101 (MQ=255)
                                                                                          cGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCg         <  2:2861915/101‑1 (MQ=255)
                                                                                          cGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCg         >  1:1897411/1‑101 (MQ=255)
                                                                                          cGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCg         >  2:169861/1‑101 (MQ=255)
                                                                                            gggCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCACGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCt       >  1:2621193/1‑101 (MQ=255)
                                                                                              gCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgc     >  1:2346379/1‑101 (MQ=255)
                                                                                               cTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgcg    <  2:1881330/101‑1 (MQ=255)
                                                                                               cTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgcg    >  1:837814/1‑101 (MQ=255)
                                                                                                 gCGGTAGCGATCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTGCGcc  >  2:5023782/1‑101 (MQ=255)
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TTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.G.CCC.ACCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTGCGCC  >  NC_014034/1948775‑1948967

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG