BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,974,337	+C	98.5%	intergenic (‑30/+25)	rpe1 ← / ← cbbM	ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,974,337	1	.→C	98.5%	2.2 0.0 ‑1.9	264	intergenic (‑30/+25)	rpe1/cbbM	ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand): new base (145/115): ref base (2/2): total (147/117)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.30e-02

Only 100 of 290 total aligned reads displayed.


GGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.GGGC.GGGGTGT.CCCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGC  >  NC_014034/1974241‑1974433


ggCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGT.cc                                                                                                <  1:1191630/101‑1 (MQ=255)


   cGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGT.ccccc                                                                                             >  1:1577751/1‑101 (MQ=255)


    gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGT.cccccc                                                                                            <  1:831726/101‑2 (MQ=255)


     aaaTCGGCGAAGTCGGCAGAAAGGATCGAGNGGGCGATCTTGATGGAACGGTAGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGT.ccccccg                                                                                           <  1:2443237/101‑3 (MQ=255)


     aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGT.ccccccg                                                                                           >  2:1423754/1‑99 (MQ=255)


     aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGT.ccccccg                                                                                           <  2:2983061/101‑3 (MQ=255)


     aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGT.ccccccg                                                                                           <  1:302425/101‑3 (MQ=255)


     aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGT.ccccccg                                                                                           >  1:1248705/1‑99 (MQ=255)


      aaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGT.ccccccgc                                                                                          >  1:1880338/1‑98 (MQ=255)


      aaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGC.GGGGGGG.TCccccccc                                                                                         >  1:1368988/1‑101 (MQ=255)


       aTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGT.ccccccgcc                                                                                         >  2:1928118/1‑97 (MQ=255)


        tCGGCGAAGTCGGCGGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGT.ccccccgccc                                                                                        <  2:3362226/101‑6 (MQ=255)


         cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTAATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGT.CCccccccccg                                                                                       >  1:2372319/1‑99 (MQ=255)


          ggCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCgg                                                                                       <  2:1574332/101‑1 (MQ=255)


          ggCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCgg                                                                                       >  2:2723027/1‑101 (MQ=255)


                 tCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCAc                                                                                <  2:1627483/101‑1 (MQ=255)


                    gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCAcggc                                                                             >  1:367811/1‑98 (MQ=255)


                    gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCGGATAGCAcggc                                                                             >  2:3493147/1‑98 (MQ=255)


                      aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGt                                                                          <  2:4438070/101‑1 (MQ=255)


                       gAAAGGATCGAGGGGTCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGtt                                                                         <  2:3922528/101‑1 (MQ=255)


                       gAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGtt                                                                         >  1:5037410/1‑101 (MQ=255)


                       gAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGtt                                                                         <  1:4529043/101‑1 (MQ=255)


                       gAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGtt                                                                         <  2:3078555/101‑1 (MQ=255)


                       gAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCGGATAGCA.CGGCGtt                                                                         >  1:1751010/1‑101 (MQ=255)


                        aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTc                                                                        >  2:4532318/1‑101 (MQ=255)


                         aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCa                                                                       <  2:3214272/101‑1 (MQ=255)


                         aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCa                                                                       >  1:113769/1‑101 (MQ=255)


                          aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAg                                                                      >  2:164191/1‑101 (MQ=255)


                          aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCGGATAGCA.CGGCGTTCAg                                                                      >  2:1763152/1‑101 (MQ=255)


                             aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGttc                                                                   >  2:1682507/1‑100 (MQ=255)


                              tCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGttca                                                                  >  1:3292746/1‑99 (MQ=255)


                              tCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGttca                                                                  <  2:1045789/101‑3 (MQ=255)


                               cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                                 >  2:2388776/1‑101 (MQ=255)


                               cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                                 >  2:4888786/1‑101 (MQ=255)


                                gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGGTTACg                                                                >  2:4840127/1‑101 (MQ=255)


                                gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGGTCACg                                                                >  2:4279317/1‑101 (MQ=255)


                                 aGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGc                                                               >  2:4036251/1‑101 (MQ=255)


                                    gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                            <  2:1880201/101‑1 (MQ=255)


                                    gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                            >  2:1556880/1‑101 (MQ=255)


                                     ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                                           <  1:1700061/101‑1 (MQ=255)


                                       cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagag                                                         >  1:1550325/1‑101 (MQ=255)


                                       cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGAAAGCA.CGGCGTTCAGTTTACCCCCatag                                                         >  2:2839248/1‑101 (MQ=255)


                                         aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCGGATAGCA.CGGCGGTCAGTTCACGCCCAGAGCg                                                       >  1:4270575/1‑101 (MQ=255)


                                           cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                                     >  1:4515269/1‑101 (MQ=255)


                                           cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                                     >  1:737383/1‑101 (MQ=255)


                                            ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                                    <  1:2723228/101‑1 (MQ=255)


                                             tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCCGAGCGAcgc                                                   >  2:1302839/1‑101 (MQ=255)


                                              gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                                  >  1:3108773/1‑101 (MQ=255)


                                              gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                                  >  2:1591421/1‑101 (MQ=255)


                                              gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                                  >  1:4067465/1‑101 (MQ=255)


                                                 ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                               >  1:1323684/1‑101 (MQ=255)


                                                    aCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGcc                                            <  2:1517953/101‑1 (MQ=255)


                                                      ggTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                                          >  2:472674/1‑101 (MQ=255)


                                                       gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                                         <  2:2621083/101‑1 (MQ=255)


                                                       gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                                         <  2:3595292/101‑1 (MQ=255)


                                                       gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                                         >  2:4340230/1‑101 (MQ=255)


                                                         cGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                                       >  1:1645936/1‑101 (MQ=255)


                                                           aaGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                                     >  2:561322/1‑101 (MQ=255)


                                                           aaGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                                     <  1:372166/101‑1 (MQ=255)


                                                            aGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGCCGCGCCAGCCCGGATaga                                    >  1:680084/1‑101 (MQ=255)


                                                                  tGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                              >  2:3472825/1‑101 (MQ=255)


                                                                  tGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                              >  1:3579162/1‑101 (MQ=255)


                                                                   gTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTc                             <  1:3246581/101‑1 (MQ=255)


                                                                    tCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCg                            <  2:2140668/101‑1 (MQ=255)


                                                                     cGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCgg                           >  1:2907605/1‑101 (MQ=255)


                                                                      ggTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGc                          <  1:4026568/101‑1 (MQ=255)


                                                                       gTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCCCCCCGGTAGCACC.GCGGTCAGGTCACGCCCCGAGCGGCGCGCCCGCCCCGGGAGAGCCTGTCgggc                         >  1:3470790/1‑99 (MQ=255)


                                                                        tctcCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt                        <  2:1291905/101‑1 (MQ=255)


                                                                        tctcCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt                        >  2:2216820/1‑101 (MQ=255)


                                                                        tctcCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt                        <  2:1384292/101‑1 (MQ=255)


                                                                         ctcCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCCCGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAta                       >  1:1292026/1‑100 (MQ=255)


                                                                         ctcCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc                       >  1:1958936/1‑101 (MQ=255)


                                                                           ccTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCTAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGt                     <  1:1409111/101‑1 (MQ=255)


                                                                           ccTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGTGCTTGTCGGCATCGt                     <  1:4256909/101‑1 (MQ=255)


                                                                            cTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt                    <  1:2084826/101‑1 (MQ=255)


                                                                            cTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt                    <  1:1113195/101‑1 (MQ=255)


                                                                             tGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGCGCCTGTCGGCATCGTTc                   >  2:4588619/1‑101 (MQ=255)


                                                                             tGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc                   <  1:2035830/101‑1 (MQ=255)


                                                                             tGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc                   >  2:1145301/1‑101 (MQ=255)


                                                                             tGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc                   >  1:3758889/1‑101 (MQ=255)


                                                                              gAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg                  >  2:422496/1‑101 (MQ=255)


                                                                              gAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg                  <  1:550868/101‑1 (MQ=255)


                                                                              gAGGGGTTGGGGCGGGGGAGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg                  >  2:810251/1‑101 (MQ=255)


                                                                               aGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg                 >  1:1472336/1‑101 (MQ=255)


                                                                                  ggttggggcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCGCGCCCGGATAGAGCTTGTCGGCATCGTTCGGGaa              >  1:4337011/11‑101 (MQ=255)


                                                                                  ggttggggcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGaa              <  2:3102015/91‑1 (MQ=255)


                                                                                     tggggcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt           <  1:587146/94‑1 (MQ=255)


                                                                                     tggggcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt           >  1:977959/8‑101 (MQ=255)


                                                                                     tggggcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt           <  1:257136/94‑1 (MQ=255)


                                                                                      ggggcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCtt          >  1:1822700/7‑101 (MQ=255)


                                                                                      ggggcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCtt          >  1:2000108/7‑101 (MQ=255)


                                                                                      ggggcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCtt          >  1:1688517/7‑101 (MQ=255)


                                                                                       gggcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCttt         <  2:2510075/96‑1 (MQ=255)


                                                                                         gcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCg       <  1:4821096/98‑1 (MQ=255)


                                                                                           gggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGaa     >  1:2790643/2‑101 (MQ=255)


                                                                                           gggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGaa     >  2:3881696/2‑101 (MQ=255)


                                                                                            ggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAAc    <  2:1822567/101‑1 (MQ=255)


                                                                                            ggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAAc    <  2:201223/101‑1 (MQ=255)


                                                                                            ggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAAc    >  2:233043/1‑101 (MQ=255)


                                                                                             gggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACg   <  1:2216975/101‑1 (MQ=255)


GGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.GGGC.GGGGTGT.CCCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGC  >  NC_014034/1974241‑1974433

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG