Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,979,921 +G 2.3% intergenic (‑37/+47) tkt1 ← / ← cbbP transketolase/phosphoribulokinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,979,9211.→G 2.3% 7.4 ‑3.8 ‑0.3215intergenic (‑37/+47)tkt1/cbbPtransketolase/phosphoribulokinase
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (97/113):  total (102/113)
Fisher's exact test strand distribution p-value = 2.28e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.49e-01

Only 100 of 258 total aligned reads displayed.

CCATCGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAG  >  NC_014034/1979821‑1980021
                                                                                                     |                                                                                                       
ccatcGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAgcggc                                                                                                          <  2:2014650/101‑1 (MQ=255)
  atcGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGcggc.gg                                                                                                       >  1:2932644/1‑101 (MQ=255)
     gCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.cc                                                                                                   <  2:2171135/101‑1 (MQ=255)
      ccAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.gcc                                                                                                  >  2:3652272/1‑101 (MQ=255)
      ccAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.ccc                                                                                                  <  1:4186273/101‑1 (MQ=255)
       cAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.g                                                                                                <  2:4453100/101‑1 (MQ=255)
          cgcCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTg                                                                                             <  1:4598543/101‑1 (MQ=255)
          cgcCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTg                                                                                             <  1:3689326/101‑1 (MQ=255)
           gcCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGTGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.ccccgggg                                                                                             >  2:2571699/1‑96 (MQ=255)
           gcCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.GCC.GGGgg                                                                                            >  1:1118165/1‑101 (MQ=255)
           gcCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGgg                                                                                            >  1:1697772/1‑101 (MQ=255)
               ggATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGCGGGG.CCC.GGGgggcg                                                                                         >  2:63446/1‑100 (MQ=255)
               ggATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.ccccctggcggc                                                                                         >  2:3067276/1‑92 (MQ=255)
                gATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTgggccgg                                                                                       <  1:3533172/101‑1 (MQ=255)
                 aTGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGggggcggg                                                                                      >  1:4927253/1‑101 (MQ=255)
                     cATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGCGGGG.CCC.GGGgggccggcccc                                                                                   >  2:2747024/1‑100 (MQ=255)
                     cATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTc                                                                                  <  2:28764/101‑1 (MQ=255)
                       ttGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGc                                                                                <  1:2368128/101‑1 (MQ=255)
                        tGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCC.GGGggcccgggcccggc                                                                                >  2:4986928/1‑97 (MQ=255)
                        tGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGcc                                                                               <  2:1167777/101‑1 (MQ=255)
                         ggCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGCGGGG.CCC.GGGgggccgggccccccc                                                                               >  2:1703430/1‑96 (MQ=255)
                           ccATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAAt                                                                            <  2:3823793/101‑1 (MQ=255)
                             aTCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.CGTGGGCCGGGCCCCGCCCATGt                                                                          >  1:3207601/1‑101 (MQ=255)
                                cgctTTTCCTGNGNNCTANCNAGATNNNNNNTGGCCCCTCCTCCCTGATGAGCCTTTCNTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCtt                                                                       <  1:4149439/101‑1 (MQ=255)
                                 gggTTTCCTGCGCCATATCGAGATCCTTCATGGCGCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTg                                                                      <  2:3985742/99‑1 (MQ=255)
                                 gcgTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTg                                                                      <  2:2722331/101‑1 (MQ=255)
                                 gcgTTTCCTGCGCCATATCGAGACCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTg                                                                      <  1:1726482/101‑1 (MQ=255)
                                  cgTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCCCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGa                                                                     <  1:578144/101‑1 (MQ=255)
                                   gTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCTGGTGCACGGGCCCCCGCCaagttgttat                                                                     >  2:1872260/1‑93 (MQ=255)
                                   gTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGGCGGGGCGCGCCAATGTCTTgta                                                                    >  2:4073491/1‑99 (MQ=255)
                                   gTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.CGTGGGCCCGGCCTCGCCCATTTCTTgta                                                                    >  2:1552239/1‑99 (MQ=255)
                                   gTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.CGGGGGGCGGGGCTCCCCACTGTCtttta                                                                    >  1:1866036/1‑98 (MQ=255)
                                    tttCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGaaa                                                                   <  2:1017762/101‑1 (MQ=255)
                                      tCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATc                                                                 <  1:1543648/101‑1 (MQ=255)
                                      tCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.CGGGGGCCGGGGCTCCCCACTTTCCTGaaaac                                                                 >  1:1659737/1‑99 (MQ=255)
                                       ccTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGGCGGGCCTCGCCAATTTCATGaaaaac                                                                >  2:3383783/1‑98 (MQ=255)
                                         tGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.tt                                                             <  2:2801594/101‑1 (MQ=255)
                                          gcgcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTc                                                            <  1:3807609/101‑1 (MQ=255)
                                           cgcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTcc                                                           <  1:749399/101‑1 (MQ=255)
                                            gcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCg                                                          <  1:1990478/101‑1 (MQ=255)
                                            gcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCg                                                          <  1:3570717/101‑1 (MQ=255)
                                              cATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGt                                                        <  2:3817450/101‑1 (MQ=255)
                                              cATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.CGTGGGCCGGGGCTCGCCCAAGTCTTTGAAACG.TTCCGGt                                                        >  2:141096/1‑101 (MQ=255)
                                              cATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.CGTGGGCCGGGCCCCGCCCAAGTCTTGAAATCG.TTCCGGt                                                        >  1:2499414/1‑101 (MQ=255)
                                              cATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.CGCGGGGCCGGCCCCCCCCAAGTCTTGAAAACG.TTCCGGt                                                        >  1:4583031/1‑101 (MQ=255)
                                               atatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGtt                                                       <  1:4805744/101‑1 (MQ=255)
                                               atatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGtt                                                       <  1:3360544/101‑1 (MQ=255)
                                               atatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGtt                                                       <  2:1067108/101‑1 (MQ=255)
                                               atatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGtt                                                       <  2:92269/101‑1 (MQ=255)
                                               atatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGAGGCAGGGCCTCGCCCATGTCTTGAAATCG.TTCCCGtt                                                       >  1:4312898/1‑101 (MQ=255)
                                                tatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTAGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTc                                                      <  1:212406/101‑1 (MQ=255)
                                                 atCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCa                                                     <  1:2039242/101‑1 (MQ=255)
                                                 atCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCa                                                     <  1:1154817/101‑1 (MQ=255)
                                                   cGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAgg                                                   <  2:2660034/101‑1 (MQ=255)
                                                   cGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGGCGGGGCCCGCCAATGTCTTGAAATCG.TTCCGGGTCAgg                                                   >  1:4130670/1‑101 (MQ=255)
                                                    gagaTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCC.GGCGGGCC.CGCCCCGCCCATCTGTTTAAATTG.TTCCCGTTCCGGc                                                  >  1:687743/1‑101 (MQ=255)
                                                    gagaTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGCCCGGCCTCGCCCATGTCTTTAAAACG.TTCCGGTTTAGGc                                                  >  2:2601190/1‑101 (MQ=255)
                                                     agaTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGGCGGGCCCCGCCAATGTCTTTGAAACG.TTTCGGGTCAGGcc                                                 >  2:3243043/1‑101 (MQ=255)
                                                      gaTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGccc                                                <  2:2382554/101‑1 (MQ=255)
                                                       aTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCcg                                               <  1:3735621/101‑1 (MQ=255)
                                                        tCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCcgc                                              <  2:4226346/101‑1 (MQ=255)
                                                         ccTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.CGGGGGCCGGGGCCCCCCCATATCTTTTAAACG.TTTCGGGTCCGGcccccc                                             >  1:2759962/1‑98 (MQ=255)
                                                          cTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.CGGGGGGCGGGCCTCGCCCATGTCTTGAAATCG.TTCCGGTTCAGGCCcgcgc                                            >  2:3740808/1‑101 (MQ=255)
                                                             cATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.GCC.CGCGGGCCCGGCCCCGCCCAAGTCTTTAAATCG.TTCCGGGTCAgggaccgcggcg                                         >  2:2320480/1‑91 (MQ=255)
                                                             cATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCcgcgcgcg                                         <  1:3274886/101‑1 (MQ=255)
                                                             cATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.CGGGGGCCGGGCCCCGCCCAAGTCTTGAAAACG.TTCCGGGTCCGGCCcccccgcg                                         >  1:3190362/1‑101 (MQ=255)
                                                              aTGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.GCC.CGGGGGCCCGGCCCCGCCCATGTCTTTAAAACGTTTCGGGTTCAGAccccccccgg                                         >  2:1322420/1‑94 (MQ=255)
                                                              aTGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGGCGGGGCCCGCCCATGTCTTGGAAGCG.TTTCGGGTCGGGgcccccgccgg                                        >  2:2280226/1‑93 (MQ=255)
                                                              aTGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGGCGGGCCTCGCCAAAGTCTTCAGATAG.TTTCCGGTCAGGCGCGCGCGCGa                                        >  2:2488178/1‑101 (MQ=255)
                                                                ggCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACg                                      <  1:4686934/101‑1 (MQ=255)
                                                                ggCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.CGTGGGCCCGGCCTCGCCAAACTCTTGAAATCT.TTTCGCTTCAGGGCCCCGCGCGagg                                      >  2:1252306/1‑99 (MQ=255)
                                                                ggCGCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACg                                      <  1:3067514/101‑1 (MQ=255)
                                                                 gCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACgg                                     <  2:580921/101‑1 (MQ=255)
                                                                  ctcctcctcCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCCGGCCCCCGCGCGGCGGc                                    >  1:2647526/1‑101 (MQ=255)
                                                                   tcctcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGGCGGcc                                   >  1:815296/1‑101 (MQ=255)
                                                                   tcctcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGGCGGGCCTCGCCAATGTCTTTAAATCG.TTCCGGTTCAGGCCCGCGCGCGGCGGcc                                   >  1:1279771/1‑101 (MQ=255)
                                                                   tcctcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.CGTGGGGCGGGCCTCGCCCATGTTTTGAAATCG.TTCCGGGTCAGGGCCGCGCGCGCCCGcc                                   >  1:2176768/1‑101 (MQ=255)
                                                                    cctcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCt                                  <  2:4805964/101‑1 (MQ=255)
                                                                    cctcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGGCGGGCCCCGCCAATGTCTTGGAATCG.TTCCGGTTCAGGCCCCCGCGCGACGGccc                                  >  2:1673982/1‑100 (MQ=255)
                                                                    cctcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGGCCGGCCCCGCCCATGTCTTGAAAACG.TTCCGGTTCAGGGCCCCGCGCGCCCGCCt                                  >  1:4442310/1‑101 (MQ=255)
                                                                     ctcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGCCGGGCCTCGCCCATGTCTTTAAATCG.TTCCGGTTCAGGCCCGCCCGCGCCGGCCtt                                 >  1:3027289/1‑101 (MQ=255)
                                                                      tcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGCCGGGCCTCGCCCATGTCTTGAAATAA.TTCCGGTTCAGGCCCGCCCGCGACgggcctc                                >  1:533799/1‑96 (MQ=255)
                                                                       cctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGGCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCCTcc                               >  1:2277942/1‑100 (MQ=255)
                                                                            cTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGGCTCGCCAATGTCTTTAAATCG.TTCCGGTTCAGGCCCCCGCGCGCCGGCCTTCGCGCAc                          >  2:4975804/1‑101 (MQ=255)
                                                                            cTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.CGGGGGGCGGGCCCCGCCCATGTCTTTAAATCG.GTCCGGGTCAGGCCCGCGCGCGGCGGCCCTCgcgccc                          >  1:1336428/1‑99 (MQ=255)
                                                                              gatgaGCCTTTCGTCCAAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCa                        <  2:3607733/101‑1 (MQ=255)
                                                                               atgaGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGGTCAGGGCCGCGGGCGGCGGCCTTCGCGGACCAg                       >  2:745304/1‑101 (MQ=255)
                                                                                tgaGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGa                      <  1:4987258/101‑1 (MQ=255)
                                                                                 gaGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGAc                     <  2:2542626/101‑1 (MQ=255)
                                                                                 gaGCCTTTCGTCCGAGCGGC.GGG.CCC.GGGGGGCCGGGGCTCGCCCATGTCTTGAAAACG.TTCCGGTTCAGGCCCCCGCGCGGCGGCCCTCGCGCACCAGAc                     >  2:2054950/1‑101 (MQ=255)
                                                                                      tttCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGGCCCGCCCACGTCTTTAAATAG.TTCCGGGTCAGGCCCGCGCGCGCCGGGCCTCGCGCACCAGCCGCCCg                >  2:828285/1‑101 (MQ=255)
                                                                                      tttCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCg                <  2:431850/101‑1 (MQ=255)
                                                                                      tttCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCg                <  1:4759882/101‑1 (MQ=255)
                                                                                      tttCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCg                <  1:223949/101‑1 (MQ=255)
                                                                                         cGTCCGGGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATc             <  2:4724379/101‑1 (MQ=255)
                                                                                            ccGAGCGGC.GGG.CCC.GGTGGCCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAgc          <  2:1941517/101‑1 (MQ=255)
                                                                                               aGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAgcggc       <  1:2447208/101‑1 (MQ=255)
                                                                                                 cggc.ggG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGt     >  2:2592031/1‑101 (MQ=255)
                                                                                                   gc.ggG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCa   >  1:3881359/1‑101 (MQ=255)
                                                                                                    c.ggG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAg  >  2:851286/1‑101 (MQ=255)
                                                                                                     |                                                                                                       
CCATCGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCC.GGTGGGCCGGGCCTCGCCAATGTCTTGAAATCG.TTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAG  >  NC_014034/1979821‑1980021

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG