Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,987,493 +G 100% coding (187/2025 nt) glgX ← glycogen debranching enzyme GlgX

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,987,4931.→G100.0% 955.7 254coding (187/2025 nt)glgXglycogen debranching enzyme GlgX
Reads supporting (aligned to +/- strand):  new base (134/118):  ref base (0/1):  total (134/120)

Only 100 of 283 total aligned reads displayed.

CCGCGCATAGGGATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCACCCGGAAC.CAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGCAAAGCTCGACCTTTTCC  >  NC_014034/1987392‑1987593
                                                                                                      |                                                                                                    
ccGCGCATAGGGATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAAc                                                                                                       >  1:2588057/1‑101 (MQ=255)
ccGCGCATAGGGATCGACGAGCAGCTTGGACGGATTGTACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAAc                                                                                                       <  1:4313144/101‑1 (MQ=255)
   cgcATAGGGATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAAcgca                                                                                                    <  1:385062/101‑4 (MQ=255)
   cgcATAGGGATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAAcgca                                                                                                    >  1:844604/1‑98 (MQ=255)
     cATAGGGATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAAcgcagg                                                                                                  >  2:1135488/1‑96 (MQ=255)
     cATAGGGATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAAcgcagg                                                                                                  >  1:2100973/1‑96 (MQ=255)
     cATAGGGATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAAcgcagg                                                                                                  >  1:1880343/1‑96 (MQ=255)
     cATAGGGATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAAcgcagg                                                                                                  >  2:4237966/1‑96 (MQ=255)
      aTAGGGATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGc                                                                                                 <  2:134995/101‑1 (MQ=255)
        aGGGATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCg                                                                                               >  2:2270721/1‑101 (MQ=255)
        aGGGATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCg                                                                                               <  1:759777/101‑1 (MQ=255)
        aGGGATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCg                                                                                               >  2:589720/1‑101 (MQ=255)
         gggATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCgg                                                                                              >  1:2044076/1‑101 (MQ=255)
         gggATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCgg                                                                                              <  1:3398377/101‑1 (MQ=255)
          ggATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCggg                                                                                             >  2:1450013/1‑101 (MQ=255)
              cgacgaGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACa                                                                                         >  2:301677/1‑101 (MQ=255)
               gacgaGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACaa                                                                                        <  1:1266847/101‑1 (MQ=255)
                acgaGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACaaa                                                                                       <  2:3540548/101‑1 (MQ=255)
                  gaGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAAcc                                                                                     >  2:4015221/1‑101 (MQ=255)
                   agcagcTTGGACGGGTTGAACCGGTGACCAATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAAccc                                                                                    >  1:234955/1‑101 (MQ=255)
                    gcagcTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACTCAGGCCGGGAACAAACCCa                                                                                   <  2:1531757/101‑1 (MQ=255)
                         ttGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCca                                                                              <  2:1774408/101‑1 (MQ=255)
                         ttGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCca                                                                              >  1:3166102/1‑101 (MQ=255)
                          tGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCcac                                                                             <  2:4868917/101‑1 (MQ=255)
                          tGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCcac                                                                             >  2:499685/1‑101 (MQ=255)
                           ggACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATNNnann                                                                            >  1:1606069/1‑99 (MQ=255)
                           ggACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCcaca                                                                            <  1:4074792/101‑1 (MQ=255)
                           ggACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCcaca                                                                            <  1:5004096/101‑1 (MQ=255)
                           ggACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCcaca                                                                            >  1:2417893/1‑101 (MQ=255)
                               gggTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATc                                                                        <  2:4657196/101‑1 (MQ=255)
                                ggTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATcc                                                                       <  1:3052534/101‑1 (MQ=255)
                                ggTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATcc                                                                       <  1:589799/101‑1 (MQ=255)
                                ggTTGAACCGGTGACCGATCTCCGGGGCCCAGGGCCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATcc                                                                       >  1:2688455/1‑101 (MQ=255)
                                 gTTGAACCGGTGACCGATCTCCGGTGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATccc                                                                      <  2:844502/101‑1 (MQ=255)
                                 gTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATccc                                                                      >  1:973668/1‑101 (MQ=255)
                                  ttGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATcccc                                                                     <  2:3289620/101‑1 (MQ=255)
                                   tGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCg                                                                    >  1:3128971/1‑101 (MQ=255)
                                   tGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCg                                                                    >  1:1361857/1‑101 (MQ=255)
                                    gAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCgg                                                                   >  1:3288695/1‑101 (MQ=255)
                                       ccGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCAc                                                                <  2:3359621/101‑1 (MQ=255)
                                            gACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGcccc                                                           >  2:1709256/1‑101 (MQ=255)
                                             aCCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCg                                                          >  2:4700363/1‑101 (MQ=255)
                                              ccGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCgg                                                         <  1:989984/101‑1 (MQ=255)
                                                gATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCa                                                       <  1:108110/101‑1 (MQ=255)
                                                 aTCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAg                                                      >  1:1780389/1‑101 (MQ=255)
                                                 aTCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAg                                                      <  2:3754280/101‑1 (MQ=255)
                                                  tctcCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAgg                                                     <  1:2636228/101‑1 (MQ=255)
                                                  tctcCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAgg                                                     <  1:4238283/101‑1 (MQ=255)
                                                   ctcCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGa                                                    >  2:1745370/1‑101 (MQ=255)
                                                   ctcCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGa                                                    <  1:3787530/101‑1 (MQ=255)
                                                     ccGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACa                                                  >  1:2240495/1‑101 (MQ=255)
                                                     ccGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACa                                                  <  2:1384203/101‑1 (MQ=255)
                                                     ccGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACa                                                  >  2:816477/1‑101 (MQ=255)
                                                      cGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACaa                                                 >  1:4142344/1‑101 (MQ=255)
                                                      cGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACaa                                                 >  2:1086485/1‑101 (MQ=255)
                                                       ggggCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAg                                                <  1:3669193/101‑1 (MQ=255)
                                                       ggggCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAg                                                <  2:234921/101‑1 (MQ=255)
                                                        gggCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGt                                               <  2:1252893/101‑1 (MQ=255)
                                                        gggCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGt                                               <  2:4583033/101‑1 (MQ=255)
                                                         ggCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTc                                              <  2:2734159/101‑1 (MQ=255)
                                                         ggCCCAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTc                                              >  2:2057830/1‑101 (MQ=255)
                                                           cccAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCgg                                            >  1:486442/1‑101 (MQ=255)
                                                            ccAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCggg                                           >  2:727663/1‑101 (MQ=255)
                                                            ccAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCggg                                           >  1:2828461/1‑101 (MQ=255)
                                                             cAGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGt                                          >  1:2771186/1‑101 (MQ=255)
                                                              aGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGtc                                         >  1:3573472/1‑101 (MQ=255)
                                                              aGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGtc                                         >  1:3890009/1‑101 (MQ=255)
                                                              aGGGTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGtc                                         <  2:449644/101‑1 (MQ=255)
                                                               gggTCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGtct                                        >  2:4282874/1‑101 (MQ=255)
                                                                  tCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCt                                     >  2:393380/1‑101 (MQ=255)
                                                                  tCCATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCt                                     >  2:5030908/1‑101 (MQ=255)
                                                                    cATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTgg                                   <  1:1546338/101‑1 (MQ=255)
                                                                    cATAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTgg                                   >  2:1901784/1‑101 (MQ=255)
                                                                     aTAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGt                                  >  2:2080274/1‑101 (MQ=255)
                                                                      tAGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTAGGGTCTCCTGGTc                                 >  1:3033741/1‑101 (MQ=255)
                                                                       aGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCa                                >  1:1708088/1‑101 (MQ=255)
                                                                       aGACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCa                                >  1:3535025/1‑101 (MQ=255)
                                                                        gACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCAc                               <  1:1849733/101‑1 (MQ=255)
                                                                        gACACGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCAc                               <  2:3128733/101‑1 (MQ=255)
                                                                            cGATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTc                           <  2:2531836/101‑1 (MQ=255)
                                                                             gATATCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCg                          <  2:3424748/101‑1 (MQ=255)
                                                                             gATATCCGTATTCCGCA.ACGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCg                          <  1:49534/101‑1 (MQ=255)
                                                                              atatCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGa                         >  2:4408447/1‑101 (MQ=255)
                                                                               tatCCGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCTGGCAGGACAAGTCGGTTCTCCTGGTCACCGTCGaa                        <  2:2215090/101‑1 (MQ=255)
                                                                                   cGTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAgg                    >  1:1754338/1‑101 (MQ=255)
                                                                                    gTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGc                   >  1:3512018/1‑101 (MQ=255)
                                                                                    gTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGc                   <  1:4700686/101‑1 (MQ=255)
                                                                                    gTATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGc                   <  2:3165851/101‑1 (MQ=255)
                                                                                     tATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGCa                  >  2:2899265/1‑101 (MQ=255)
                                                                                     tATTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGCa                  >  2:2163629/1‑101 (MQ=255)
                                                                                      aTTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGCaa                 <  2:4776690/101‑1 (MQ=255)
                                                                                      aTTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGCaa                 <  1:2936684/101‑1 (MQ=255)
                                                                                      aTTCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGCaa                 >  2:1575149/1‑101 (MQ=255)
                                                                                        tCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGCAAAg               >  2:1489352/1‑101 (MQ=255)
                                                                                        tCCGCA.CCGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGCAAAg               <  2:294187/101‑1 (MQ=255)
                                                                                             caccGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCCCGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGCAAAGCTCg           <  2:2417719/99‑1 (MQ=255)
                                                                                             caccGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGCAAAGCTCg           <  1:4438104/99‑1 (MQ=255)
                                                                                              accGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGCAAAGCTCGa          <  1:4652649/100‑1 (MQ=255)
                                                                                              accGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGCAAAGCTCGa          >  1:2507078/2‑101 (MQ=255)
                                                                                                cGGAACGCAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTAGGGTCTCCTGGTCACCGTCGAAAAGGCAAAGCTCCAcc        >  1:1165877/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
CCGCGCATAGGGATCGACGAGCAGCTTGGACGGGTTGAACCGGTGACCGATCTCCGGGGCCCAGGGTCCATAGACACGATATCCGTATTCCGCACCCGGAAC.CAGGCCGGGAACAAACCCATGCCACACATCCCCGGCACGCCCCGGCAGGACAAGTCGGGTCTCCTGGTCACCGTCGAAAAGGCAAAGCTCGACCTTTTCC  >  NC_014034/1987392‑1987593

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG