Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,044,155 +C 7.2% intergenic (+28/+149) typA → / ← potG1 GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,044,1550.→C 7.2% 47.4 ‑2.6 ‑0.7256intergenic (+28/+149)typA/potG1GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG
Reads supporting (aligned to +/- strand):  new base (11/5):  ref base (106/131):  total (119/137)
Fisher's exact test strand distribution p-value = 7.30e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.15e-01

Only 100 of 286 total aligned reads displayed.

GCCGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCTTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCG  >  NC_014034/2044055‑2044252
                                                                                                                                                                                                      
 ccGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtt                                                                                                  >  1:1438606/1‑101 (MQ=255)
  cGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCttt                                                                                                 <  2:3650234/101‑1 (MQ=255)
    aaGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCttttt                                                                                               <  1:519841/101‑1 (MQ=255)
      gATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtttttca                                                                                             >  2:232935/1‑99 (MQ=255)
       aTCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtttttcat                                                                                            <  2:2136819/101‑4 (MQ=255)
         cGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTc                                                                                         <  1:2266872/101‑1 (MQ=255)
          gTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCg                                                                                        <  1:2532425/101‑1 (MQ=255)
          gTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCg                                                                                        >  2:1483436/1‑101 (MQ=255)
           tGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGt                                                                                       >  1:1746932/1‑101 (MQ=255)
            gCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTg                                                                                      >  2:2285026/1‑101 (MQ=255)
                cTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGa                                                                                  <  1:763056/101‑1 (MQ=255)
                   cgcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATg                                                                               <  2:1233345/101‑1 (MQ=255)
                    gcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGa                                                                              <  1:4408024/101‑1 (MQ=255)
                     cAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGaa                                                                             <  2:1414950/101‑1 (MQ=255)
                     cAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGaa                                                                             >  2:318752/1‑101 (MQ=255)
                     cAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGaa                                                                             <  2:721549/101‑1 (MQ=255)
                     cAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGaa                                                                             <  2:873173/101‑1 (MQ=255)
                       aGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGGTCTGCCCCCC.CTTTTCATTCGTGCCGAATGAAAt                                                                           >  2:4387915/1‑101 (MQ=255)
                       aGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAAt                                                                           <  2:312121/101‑1 (MQ=255)
                       aGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAAt                                                                           <  1:3774399/101‑1 (MQ=255)
                       aGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAAt                                                                           <  1:3455804/101‑1 (MQ=255)
                        gCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAAtt                                                                          <  1:4444588/101‑1 (MQ=255)
                        gCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAAtt                                                                          <  2:4619559/101‑1 (MQ=255)
                         cGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTc                                                                         <  1:1471168/101‑1 (MQ=255)
                           gCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCAc                                                                       <  2:428044/101‑1 (MQ=255)
                            cTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCAcc                                                                      >  2:3409163/1‑101 (MQ=255)
                            cTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCAcc                                                                      >  2:3695158/1‑101 (MQ=255)
                              tCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTc                                                                    >  1:4904836/1‑101 (MQ=255)
                               cTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcc                                                                   >  1:3935206/1‑101 (MQ=255)
                               cTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcc                                                                   <  2:1137431/101‑1 (MQ=255)
                                tGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTccc                                                                  <  1:1245685/101‑1 (MQ=255)
                                 ggACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcccc                                                                 <  1:4115814/101‑1 (MQ=255)
                                   gtccGCACGACCGCAAACGCGCCTCGCGCTAGGAATAAGCCGACAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcccccc                                                               <  2:2043478/99‑1 (MQ=255)
                                   aCCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcccccc                                                               <  1:594592/101‑1 (MQ=255)
                                   aCCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcccccc                                                               >  1:4194162/1‑101 (MQ=255)
                                    cccGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCg                                                              <  2:4653875/101‑1 (MQ=255)
                                       gCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCgggc                                                           >  2:3326617/1‑100 (MQ=255)
                                       gCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGa                                                           <  2:1841822/101‑1 (MQ=255)
                                        cACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGat                                                          <  1:3912044/101‑1 (MQ=255)
                                         aCGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGata                                                         <  2:236091/101‑1 (MQ=255)
                                          cGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGatat                                                        >  1:2466101/1‑101 (MQ=255)
                                            aGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATAttt                                                      <  2:1385791/101‑1 (MQ=255)
                                             gcgcAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTg                                                     <  1:413222/101‑1 (MQ=255)
                                              cgcAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGa                                                    <  2:4515278/101‑1 (MQ=255)
                                               gcAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCGCTGCCCCCC.TTTTTCATTTGGGGCCAATGAAATTCATCTCCCCCCGGGACATTTGAg                                                   >  1:2751684/1‑101 (MQ=255)
                                                  aaCGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCa                                                <  2:1438479/101‑1 (MQ=255)
                                                     gcgcCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGa                                             >  2:2024335/1‑101 (MQ=255)
                                                      cgcCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGAt                                            <  2:4689107/101‑1 (MQ=255)
                                                      cgcCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCAGTCGTGCCGAATGAAATTCACCTACCCCCGGGATATTTGAGGCAAGAt                                            >  2:2861444/1‑101 (MQ=255)
                                                       gcCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATa                                           <  1:4535979/101‑1 (MQ=255)
                                                       gcCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATa                                           >  1:4672065/1‑101 (MQ=255)
                                                       gcCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATa                                           <  1:318805/101‑1 (MQ=255)
                                                         cTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATaaa                                         >  2:2652392/1‑101 (MQ=255)
                                                          tCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAg                                        <  1:4946089/101‑1 (MQ=255)
                                                          tCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAg                                        <  2:1085735/101‑1 (MQ=255)
                                                            gcgcTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGGTATTTGAGGCAAGATAAAGAc                                      >  2:4479391/1‑101 (MQ=255)
                                                            gcgcTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGAc                                      <  1:1052417/101‑1 (MQ=255)
                                                             cgcTCGGAATAAGGCGTCAAAAGGGGCGCTCTGCCCCCC.TTTGTCATTCGTGCCGAATGAAATTCTCCCCCCCCCGGGATATTTGAGGCAAGATAAAGACt                                     <  2:1632691/101‑1 (MQ=38)
                                                              gcTGGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCCCCTCCCCCCGGGATATTTGAGGCAAGATAAAGACtt                                    <  1:4064189/101‑1 (MQ=255)
                                                              gcTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACtt                                    <  2:3218926/101‑1 (MQ=255)
                                                               cTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCTTTTTT.ATTCGTGCCGAATGAAATTTACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTg                                   >  1:939917/1‑101 (MQ=255)
                                                               cTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTg                                   <  1:2706451/101‑1 (MQ=255)
                                                               cTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTg                                   <  2:408451/101‑1 (MQ=255)
                                                                 cGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCTTTTTT.ATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTgaa                                 >  1:1527906/1‑99 (MQ=255)
                                                                 cGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCg                                 >  1:1363972/1‑101 (MQ=255)
                                                                 aggAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCg                                 <  1:2080522/100‑1 (MQ=255)
                                                                  ggAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGa                                <  1:3695436/101‑1 (MQ=255)
                                                                  ggAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGa                                <  2:2924781/101‑1 (MQ=255)
                                                                  ggAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGa                                >  1:1805251/1‑101 (MQ=255)
                                                                   gAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAg                               >  2:2503726/1‑101 (MQ=255)
                                                                     aTAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAggg                             >  1:1135518/1‑100 (MQ=255)
                                                                     aTAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAggg                             >  1:1816670/1‑100 (MQ=255)
                                                                     aTAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGgt                             >  1:4508892/1‑101 (MQ=255)
                                                                       aaGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGgtgt                           >  1:2369082/1‑101 (MQ=255)
                                                                       aaGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGgtgt                           >  1:3393609/1‑101 (MQ=255)
                                                                        aGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGtt                          >  1:1631271/1‑101 (MQ=255)
                                                                            gTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttcttc                      <  1:1961191/101‑1 (MQ=255)
                                                                            gTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttcttc                      >  2:1879555/1‑101 (MQ=255)
                                                                             tCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCa                     <  1:4035630/101‑1 (MQ=255)
                                                                              cAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCAt                    >  2:3846401/1‑101 (MQ=255)
                                                                              cAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCAt                    >  1:3069423/1‑101 (MQ=255)
                                                                              cAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCAt                    <  2:1547173/101‑1 (MQ=255)
                                                                               aaaaGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATc                   >  1:1948920/1‑101 (MQ=255)
                                                                               aaaaGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATc                   <  1:4479711/101‑1 (MQ=255)
                                                                                aaaGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCt                  >  1:35639/1‑101 (MQ=255)
                                                                                aaaGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCt                  >  1:1456611/1‑101 (MQ=255)
                                                                                  aGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCttt                <  1:139086/101‑1 (MQ=255)
                                                                                  aGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGCGTTCTTCATCttt                >  1:826378/1‑101 (MQ=255)
                                                                                   ggggggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTc               >  2:4827829/1‑101 (MQ=255)
                                                                                     ggggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTg             >  1:701425/1‑101 (MQ=255)
                                                                                      gggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGc            >  2:1722237/1‑101 (MQ=255)
                                                                                       ggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCa           <  1:428963/101‑1 (MQ=255)
                                                                                        gCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCaa          <  1:3872769/101‑1 (MQ=255)
                                                                                         ctctGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCaaa         >  2:3540890/1‑101 (MQ=255)
                                                                                          tctGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAg        <  2:3302213/101‑1 (MQ=255)
                                                                                          tctGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAg        <  1:1385829/101‑1 (MQ=255)
                                                                                           ctGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGa       >  2:191463/1‑101 (MQ=255)
                                                                                            gatcccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGa       <  2:5016551/98‑1 (MQ=255)
                                                                                             tgcccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGAc      >  2:3996779/3‑101 (MQ=255)
                                                                                                ccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACcgc   <  2:2218031/101‑1 (MQ=255)
                                                                                                                                                                                                      
GCCGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCTTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCG  >  NC_014034/2044055‑2044252

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG