Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,047,090 +C 86.6% coding (20/879 nt) potH1 ← polyamine ABC transporter permease PotH

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,047,0901.→C86.6% 100.3 ‑2.9 ‑0.8238coding (20/879 nt)potH1polyamine ABC transporter permease PotH
Reads supporting (aligned to +/- strand):  new base (88/118):  ref base (20/12):  total (108/130)
Fisher's exact test strand distribution p-value = 5.50e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.67e-01

Only 100 of 261 total aligned reads displayed.

CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.G.CCACCG.CG.GG.TGGAGATGCCGACATACTCTATT.CT.CC.TCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGG  >  NC_014034/2046992‑2047180
                                                                                                     |                                                                                                 
cgacgaTGGTCACCGGGGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGGCCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACcg.c                                                                                                  <  2:499743/101‑1 (MQ=255)
cgacgaGGGTCCCCGGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACcg.c                                                                                                  <  2:961593/101‑1 (MQ=255)
 gacgaTGGTCCCCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACcg.cc                                                                                                 <  2:2887897/101‑2 (MQ=255)
   cgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Cc.gg                                                                                              >  1:825455/1‑101 (MQ=255)
   cgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Cc.gg                                                                                              >  1:2310880/1‑101 (MQ=255)
    gaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Cc.ggg                                                                                             >  2:561584/1‑100 (MQ=255)
    gaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Cc.ggg                                                                                             >  1:4657658/1‑100 (MQ=255)
       ggTCACCGGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Cc.gggttg                                                                                          <  1:954182/101‑5 (MQ=255)
       ggTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Cc.gggttg                                                                                          >  2:370855/1‑97 (MQ=255)
       ggTCACCAGCGGGGCCGCCAGCAACAGAACTGCAGAGATCGGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Cc.gggttg                                                                                          <  2:4781307/101‑5 (MQ=255)
        gTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Cc.gggttgg                                                                                         <  2:3212620/101‑6 (MQ=255)
        gTCACCAGCGGGGCCGCCAGCAACAGAACTGCAGAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Cc.gggttgg                                                                                         <  1:4368836/101‑6 (MQ=255)
         tCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Cc.gggttgga                                                                                        <  1:1943555/101‑7 (MQ=255)
          cACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Cc.gggttggag                                                                                       >  2:4412926/1‑94 (MQ=255)
          cACCAGCGGGGCCGCAAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Cc.gggttggag                                                                                       >  1:4255795/1‑94 (MQ=255)
           cccAGGGGGTCCGCCAGCAACAGAACTGCATAGATCAGGGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Cc.gggttggaga                                                                                      <  2:3616695/100‑9 (MQ=255)
            ccAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGAt                                                                                      <  2:3026011/101‑1 (MQ=255)
             cAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATg                                                                                     >  1:1421761/1‑101 (MQ=255)
                cGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCg                                                                                  >  2:1205216/1‑101 (MQ=255)
                 ggggCCGCCAGCAACAGAACTGCATAGATCAGCTTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGa                                                                                 <  1:2157679/101‑1 (MQ=255)
                 ggggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGa                                                                                 >  1:4472460/1‑101 (MQ=255)
                  gggCCGCCAGCAACAGAACTGCATGGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGAc                                                                                <  2:4916149/101‑1 (MQ=255)
                  gggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGAc                                                                                >  1:2985709/1‑101 (MQ=255)
                   ggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACa                                                                               <  1:2044288/101‑1 (MQ=255)
                    gccgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACAt                                                                              >  1:1249514/1‑101 (MQ=255)
                     ccgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATa                                                                             >  1:4968545/1‑101 (MQ=255)
                      cgccAGCAACGGAACTGCATAGATCAGCGTGGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATAc                                                                            <  2:1428141/101‑1 (MQ=255)
                       gccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATAct                                                                           <  1:3215417/101‑1 (MQ=255)
                        ccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATActc                                                                          >  1:2644505/1‑101 (MQ=255)
                        ccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATActc                                                                          >  2:176170/1‑101 (MQ=255)
                         cAGCAACAGAACTGCATAGATCAGCTTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATActct                                                                         <  2:3850188/101‑1 (MQ=255)
                         cAGCAACAGAACTGCATAGATCAGCTTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATActct                                                                         <  2:1015211/101‑1 (MQ=255)
                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATActct                                                                         >  1:4504941/1‑101 (MQ=255)
                           gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTAt                                                                       <  2:3268391/101‑1 (MQ=255)
                           gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTAt                                                                       <  1:4579655/101‑1 (MQ=255)
                            cAACAGAACTTCATAGATCGGTGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTAtt                                                                      <  2:4833725/101‑1 (MQ=255)
                            cAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTAtt                                                                      >  1:2943204/1‑101 (MQ=255)
                             aaCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATt.c                                                                    >  1:3301064/1‑101 (MQ=255)
                                aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.ct.cc                                                                >  2:2857342/1‑101 (MQ=255)
                                 gAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.ct.ccc                                                               >  2:3376877/1‑100 (MQ=255)
                                 gAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.ct.ccc                                                               <  2:1679747/101‑2 (MQ=255)
                                 aaaCTGCATAGATCAGCGTTGGGCTGCCCATCAACCAGCCGCTGCCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.ct.ccc                                                               <  2:2934111/100‑2 (MQ=255)
                                  atcTGCATAGATCAGCGTTGGGCTCACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.ct.ccct                                                              <  2:2985481/99‑3 (MQ=255)
                                  aaCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.ct.ccct                                                              >  2:3656669/1‑99 (MQ=255)
                                   aCTGCATAGATCAGCGTGGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.ct.ccctc                                                             <  2:4352067/101‑4 (MQ=255)
                                    cTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.ct.ccctca                                                            <  1:3673363/101‑5 (MQ=255)
                                       cATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAgcg                                                         >  1:174500/1‑101 (MQ=255)
                                        aTAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAgcgc                                                        <  2:3991209/101‑1 (MQ=255)
                                        aTAGAGCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAgcgc                                                        <  1:1878448/101‑1 (MQ=255)
                                            accAGCGTTGGGCGGGCGATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCCCCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAgcgcgtct                                                    <  1:2061320/99‑5 (MQ=255)
                                              cAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCa                                                  >  2:1484152/1‑101 (MQ=255)
                                              cAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCa                                                  >  1:1668371/1‑101 (MQ=255)
                                               aGCGTTGGGCCGACCATGAACCAGCCGCTGGTCGCCTCCGAGCGCAA.CAACCGCCG.GGATCGAGATGCCGGCATGCTCCATT.CA.CCGTCAgcgcgcctccg                                                 >  2:1650351/1‑94 (MQ=255)
                                               aGCGTGGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCA.g                                                <  2:1415739/101‑1 (MQ=255)
                                                gCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCA.gg                                               >  2:2320189/1‑100 (MQ=255)
                                                    tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCA.ggcacg                                           >  1:4817164/1‑96 (MQ=255)
                                                       gCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGttt                                         <  1:1235892/101‑1 (MQ=255)
                                                       gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGttt                                         >  2:1555505/1‑101 (MQ=255)
                                                       gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGttt                                         >  2:3737167/1‑101 (MQ=255)
                                                       gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGttt                                         >  1:3127894/1‑101 (MQ=255)
                                                       gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGttt                                         <  2:2516443/101‑1 (MQ=255)
                                                        cTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTg                                        >  2:1815788/1‑101 (MQ=255)
                                                         ggcccATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTgg                                       <  2:348688/98‑1 (MQ=255)
                                                             cATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCa                                   >  2:497589/1‑101 (MQ=255)
                                                               tGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCaaa                                 <  1:1471119/101‑1 (MQ=255)
                                                                gAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAAt                                >  2:2604044/1‑101 (MQ=255)
                                                                 aaCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGGCATACTCTATTCCC.CC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAAtt                               <  1:4902728/101‑1 (MQ=255)
                                                                 aaCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAAtt                               >  2:502997/1‑101 (MQ=255)
                                                                  aCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAAttt                              >  1:792731/1‑101 (MQ=255)
                                                                  aCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAAttt                              <  1:218591/101‑1 (MQ=255)
                                                                    cAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGc                            >  1:1028874/1‑101 (MQ=255)
                                                                         gCTGGCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                       >  2:1532583/1‑101 (MQ=255)
                                                                            ggCCGCCTCCGAGCGC.GCCCCCCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                    <  2:1346842/101‑1 (MQ=255)
                                                                            ggCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                    >  2:3188475/1‑101 (MQ=255)
                                                                            ggCCGCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                    <  1:611239/101‑1 (MQ=255)
                                                                            ggCCCCCTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                    <  2:4235233/101‑1 (MQ=255)
                                                                             gccgccGCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGttt                   <  2:2304045/101‑1 (MQ=255)
                                                                              ccgccTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                  <  1:4029327/101‑1 (MQ=255)
                                                                              ccgccTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                  >  1:3926142/1‑101 (MQ=255)
                                                                              ccgccTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                  <  1:2914318/101‑1 (MQ=255)
                                                                               cgccTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCg                 <  2:4255478/101‑1 (MQ=255)
                                                                                 ccTCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGc               <  1:1786276/101‑1 (MQ=255)
                                                                                   tCCGAGCGC.GCCCACCGCCGTGT.TGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCag             <  1:4144758/101‑1 (MQ=255)
                                                                                   tCCGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCag             <  2:2963621/101‑1 (MQ=255)
                                                                                    ccGAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga            >  2:2179785/1‑101 (MQ=255)
                                                                                      gAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCagaga          >  2:4604822/1‑100 (MQ=255)
                                                                                      gAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg          <  2:4056995/101‑1 (MQ=255)
                                                                                      gAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg          >  2:619151/1‑101 (MQ=255)
                                                                                      gAGCGC.GCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg          >  2:4698439/1‑101 (MQ=255)
                                                                                        gcgc.gcCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaa        <  2:694245/101‑1 (MQ=255)
                                                                                        gcgc.gcCCACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaa        <  2:126903/101‑1 (MQ=255)
                                                                                          cgcgcccACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa       <  1:1509187/96‑1 (MQ=255)
                                                                                          cgcgcccACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa       <  1:1651614/96‑1 (MQ=255)
                                                                                          cgcgcccACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa       <  2:409726/96‑1 (MQ=255)
                                                                                          cgcgcccACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa       <  2:3119928/96‑1 (MQ=255)
                                                                                           gcgcccACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAg      <  2:2792702/97‑1 (MQ=255)
                                                                                            cgcccACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgg     <  1:1076284/98‑1 (MQ=255)
                                                                                             gcccACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgag    >  1:3403437/3‑99 (MQ=255)
                                                                                              cccACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgggg   <  1:2490523/100‑1 (MQ=255)
                                                                                               ccACCGCCG.GGTTGGAGATGCCGACATACTCTATT.CTCCC.TCGGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg  <  2:1996728/101‑1 (MQ=255)
                                                                                                     |                                                                                                 
CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.G.CCACCG.CG.GG.TGGAGATGCCGACATACTCTATT.CT.CC.TCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGG  >  NC_014034/2046992‑2047180

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG