Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,047,119 +C 100% intergenic (‑10/+158) potH1 ← / ← RCAP_rcc01896 polyamine ABC transporter permease PotH/GntR family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,047,1191.→C100.0% 888.7 233intergenic (‑10/+158)potH1/RCAP_rcc01896polyamine ABC transporter permease PotH/GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (104/128):  ref base (0/1):  total (104/129)

Only 100 of 258 total aligned reads displayed.

CAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.G.CCACCG.CGGG.TGGAGATGCCGACATACTCTATT.CT.CC.TCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAA  >  NC_014034/2047023‑2047212
                                                                                                      |                                                                                                
aaGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATt.ct.c                                                                                                 <  1:2575705/100‑1 (MQ=255)
 aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.ct.cc                                                                                                >  2:2857342/1‑101 (MQ=255)
  gAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.ct.ccc                                                                                               >  2:3376877/1‑100 (MQ=255)
    aCTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.ct.ccctc                                                                                             <  1:1138280/101‑4 (MQ=255)
    aCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.ct.ccctc                                                                                             <  2:4986770/101‑4 (MQ=255)
    aCTGCATAGATCAGCGTGGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.ct.ccctc                                                                                             <  2:4352067/101‑4 (MQ=255)
     cTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.ct.ccctca                                                                                            <  1:3673363/101‑5 (MQ=255)
       gaaTAGATCAGCGTTGGGTTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAgc                                                                                          <  2:1481851/99‑1 (MQ=255)
       gCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAgc                                                                                          <  2:513339/101‑1 (MQ=255)
       gCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAgc                                                                                          <  1:2947524/101‑1 (MQ=255)
        cATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAgcg                                                                                         >  1:174500/1‑101 (MQ=255)
        cATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAgcg                                                                                         <  2:4472141/101‑1 (MQ=255)
         aTAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGACCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATGCTCTATT.ct.ccctccgcgc                                                                                        >  2:1509057/1‑93 (MQ=255)
           aGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAgcccgt                                                                                      >  1:2910458/1‑97 (MQ=255)
              tCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAgcgcgtctc                                                                                   <  2:1237512/101‑6 (MQ=255)
               cAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCa                                                                                  >  1:1497613/1‑101 (MQ=255)
                aGCGTTGGGCCGACCATGAACCAGCCGCTGGTCGCCTCCGAGCGCAA.CAACCGCCGGGATCGAGATGCCGGCATGCTCCATT.CA.CCGTCAgcgcgcctccg                                                                                 >  2:1650351/1‑94 (MQ=255)
                 gCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCA.gg                                                                               <  2:3715667/101‑2 (MQ=255)
                 gCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCA.gg                                                                               >  2:3173573/1‑100 (MQ=255)
                    ttGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCA.ggcgc                                                                            >  2:3536387/1‑97 (MQ=255)
                    ttGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCA.ggcgc                                                                            >  1:1832931/1‑97 (MQ=255)
                     tGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCA.ggcgcg                                                                           <  2:1716983/101‑6 (MQ=255)
                      gggCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGt                                                                           >  2:1786143/1‑101 (MQ=255)
                       ggCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGtt                                                                          <  1:120745/101‑1 (MQ=255)
                        gCTGCCCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGttt                                                                         <  2:2310716/101‑1 (MQ=255)
                        gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGttt                                                                         >  1:3127894/1‑101 (MQ=255)
                        gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGttt                                                                         >  2:1555505/1‑101 (MQ=255)
                        gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGttt                                                                         >  2:3737167/1‑101 (MQ=255)
                         cTGCCCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTg                                                                        <  1:4944586/101‑1 (MQ=255)
                         cTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTg                                                                        <  1:2238113/101‑1 (MQ=255)
                         cTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTg                                                                        >  2:1815788/1‑101 (MQ=255)
                         cTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTg                                                                        <  1:1413966/101‑1 (MQ=255)
                          tGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTgg                                                                       <  1:3476990/101‑1 (MQ=255)
                          tGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTgg                                                                       <  1:2052018/101‑1 (MQ=255)
                          ggcccATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTgg                                                                       <  2:348688/98‑1 (MQ=255)
                             ccATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGcc                                                                    >  1:694334/1‑101 (MQ=255)
                              cATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCa                                                                   >  1:1910762/1‑101 (MQ=255)
                              cATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTAGGCCa                                                                   >  2:2487/1‑101 (MQ=255)
                                 gacccAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAAt                                                                <  2:2942976/98‑1 (MQ=255)
                                 gAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAAt                                                                <  1:1074684/101‑1 (MQ=255)
                                 gAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAAt                                                                >  1:4865084/1‑101 (MQ=255)
                                   aCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAAttt                                                              <  1:218591/101‑1 (MQ=255)
                                      aGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGca                                                           >  2:211820/1‑101 (MQ=255)
                                       gCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGcac                                                          >  2:4308464/1‑101 (MQ=255)
                                          gCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                                                       >  2:1532583/1‑101 (MQ=255)
                                             ggCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                                                    <  2:4660852/101‑1 (MQ=255)
                                             ggCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                                                    <  1:4114890/101‑1 (MQ=255)
                                             ggCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                                                    <  1:1780271/101‑1 (MQ=255)
                                               ccgccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                                                  >  1:3926142/1‑101 (MQ=255)
                                               ccgccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                                                  <  2:5026180/101‑1 (MQ=255)
                                                cgccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATTCTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCg                                                 <  1:4614650/101‑1 (MQ=255)
                                                cgccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCg                                                 <  2:4255478/101‑1 (MQ=255)
                                                  ccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGc                                               <  1:536937/101‑1 (MQ=255)
                                                  ccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGc                                               <  2:3257914/101‑1 (MQ=255)
                                                  ccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGc                                               <  2:2969338/101‑1 (MQ=255)
                                                  ccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGc                                               <  1:889223/101‑1 (MQ=255)
                                                    tCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCag                                             <  2:3199241/101‑1 (MQ=255)
                                                     ccGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga                                            >  2:2179785/1‑101 (MQ=255)
                                                       gAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg                                          <  2:4056995/101‑1 (MQ=255)
                                                       gAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg                                          >  2:4698439/1‑101 (MQ=255)
                                                       gAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg                                          >  2:619151/1‑101 (MQ=255)
                                                         gcgc.gcCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaa                                        <  2:694245/101‑1 (MQ=255)
                                                         gcgc.gcCCACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaa                                        <  2:126903/101‑1 (MQ=255)
                                                           cggcaccACCGTCGGGTTGGAGATGCCGACATCCCCTATT.CTCCC.GCAGCGCGAATCAGGCGCGTTTGCGCCAAATTTGGACAAGGTTTCGGCAGCGGaaa                                       <  2:4878383/96‑1 (MQ=255)
                                                           cgcgcccACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa                                       <  1:1651614/96‑1 (MQ=255)
                                                             cgcccACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgg                                     <  2:4005704/98‑1 (MQ=255)
                                                               cccACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgggg                                   <  1:3385323/100‑1 (MQ=255)
                                                                ccACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCGGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg                                  <  2:1996728/101‑1 (MQ=255)
                                                                ccACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg                                  <  2:386573/101‑1 (MQ=255)
                                                                ccACCGCCGGGTTGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg                                  <  2:1386770/101‑1 (MQ=255)
                                                                  accgccgggttGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGAc                                <  1:2620666/91‑1 (MQ=255)
                                                                   ccgccgggttGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACg                               <  1:211854/92‑1 (MQ=255)
                                                                       cgggttGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGc                           >  1:4828480/6‑101 (MQ=255)
                                                                       cgggttGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGc                           <  1:3705229/96‑1 (MQ=255)
                                                                        gggttGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGcc                          >  2:1016996/5‑101 (MQ=255)
                                                                         ggttGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGccc                         >  1:247529/4‑101 (MQ=255)
                                                                         ggttGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGccc                         >  1:394519/4‑101 (MQ=255)
                                                                          gttGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGcccc                        <  2:2235961/99‑1 (MQ=255)
                                                                          gttGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGcccc                        >  2:4970751/3‑101 (MQ=255)
                                                                            tGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCgg                      <  1:619230/101‑1 (MQ=255)
                                                                            tGGAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCgg                      >  2:4235048/1‑101 (MQ=255)
                                                                             ggAGATGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGc                     >  2:3306076/1‑101 (MQ=255)
                                                                              gagaTGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCt                    >  2:4632549/1‑101 (MQ=255)
                                                                                gaTGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTg                  <  2:2774838/101‑1 (MQ=255)
                                                                                gaTGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTg                  >  1:1329854/1‑101 (MQ=255)
                                                                                  tGCCGACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCg                <  1:1666455/101‑1 (MQ=255)
                                                                                      gACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCGCAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCt            <  1:1523863/101‑1 (MQ=255)
                                                                                      gACATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCt            >  1:1237101/1‑101 (MQ=255)
                                                                                       aCATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTg           <  2:963079/101‑1 (MQ=255)
                                                                                       aCATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTg           >  2:3009343/1‑101 (MQ=255)
                                                                                       aCATACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTg           >  1:4722932/1‑101 (MQ=255)
                                                                                         aTACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGtt         >  1:100708/1‑101 (MQ=255)
                                                                                         aTACTATATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTggt         >  2:1664100/1‑99 (MQ=255)
                                                                                          tACTCTATT.CTCCC.TCAGCGCGTCTCAGGCGCGATTGCGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGttt        <  1:4324355/101‑1 (MQ=255)
                                                                                           aCTCTATTCCC.CC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGCCGATGCCCCGGCTTGCGGCCTGTTTc       <  1:777553/101‑1 (MQ=255)
                                                                                            ctctATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCg      >  2:4161806/1‑101 (MQ=255)
                                                                                              ctATT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCggg    >  1:2372779/1‑101 (MQ=255)
                                                                                                aTT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGGTTGATGCCAGGTGCGGGaa  >  2:2517614/1‑101 (MQ=255)
                                                                                                aTT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGaa  >  1:4569411/1‑101 (MQ=255)
                                                                                                aTT.CTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGaa  <  1:4234850/101‑1 (MQ=255)
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CAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.G.CCACCG.CGGG.TGGAGATGCCGACATACTCTATT.CT.CC.TCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAA  >  NC_014034/2047023‑2047212

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG