Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,057,825 +C 78.8% intergenic (‑591/‑311) RCAP_rcc01900 ← / → RCAP_rcc01901 hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,057,8251.→C78.8% 133.7 ‑0.2 ‑0.3189intergenic (‑591/‑311)RCAP_rcc01900/RCAP_rcc01901hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (86/62):  ref base (24/16):  total (111/78)
Fisher's exact test strand distribution p-value = 8.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.97e-01

Only 100 of 222 total aligned reads displayed.

AAAATTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CCGCG.CGC.AGC.G.TTCCGAACCGGCATCAATCTTGG.C.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057725‑2057913
                                                                                                     |                                                                                                
aaaaTTTGTCCTGTTTACCGCCCATTACCCCTGCTTCGGCGCGCGGGCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAt                                                                                                   <  2:4210728/101‑1 (MQ=255)
aaaaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAt                                                                                                   >  1:2540906/1‑101 (MQ=255)
aaaaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAt                                                                                                   >  2:3877915/1‑101 (MQ=255)
 aaaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.c                                                                                                 <  2:3605153/101‑1 (MQ=255)
   aTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.ccc                                                                                               >  1:2817696/1‑100 (MQ=255)
   aTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.ccc                                                                                               <  2:2102320/101‑2 (MQ=255)
     ttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGGGGCAAT.cccgc                                                                                             >  1:4820077/1‑98 (MQ=255)
     ttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.cccgc                                                                                             >  1:3128032/1‑98 (MQ=255)
      tGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGCCAAT.cccgcg                                                                                            >  2:1122443/1‑97 (MQ=255)
      tGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.cccgcg                                                                                            >  2:1461404/1‑97 (MQ=255)
        tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgcc                                                                                        >  1:4722069/1‑100 (MQ=255)
         ccTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.cccccgccg                                                                                         >  1:1889405/1‑94 (MQ=255)
          cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGc                                                                                     >  1:1743170/1‑101 (MQ=255)
           tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGca                                                                                    <  1:4032995/101‑2 (MQ=255)
           tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGca                                                                                    >  2:106946/1‑100 (MQ=255)
           tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGca                                                                                    >  2:2317072/1‑100 (MQ=255)
           tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGca                                                                                    <  1:530923/101‑2 (MQ=255)
            gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaa                                                                                   >  2:2387183/1‑99 (MQ=255)
             tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaag                                                                                  <  1:556530/101‑4 (MQ=255)
             tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaag                                                                                  >  2:793042/1‑98 (MQ=255)
              ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagc                                                                                 >  2:3323784/1‑97 (MQ=255)
               tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGGGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcg                                                                                >  1:2372164/1‑96 (MQ=255)
               tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCCGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcg                                                                                >  2:3240113/1‑96 (MQ=255)
                 ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtt                                                                              >  1:332026/1‑94 (MQ=255)
                  cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgttt                                                                             <  1:813496/101‑9 (MQ=255)
                  cGCCCATTAACCCTGCTACGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgttt                                                                             <  2:4537891/101‑9 (MQ=255)
                    cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtttcc                                                                           <  1:3357320/101‑11 (MQ=255)
                    cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtttcc                                                                           <  1:3675029/101‑11 (MQ=255)
                          aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCTGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCg                                                                        >  1:1069702/1‑101 (MQ=255)
                          aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCg                                                                        >  1:2723847/1‑101 (MQ=255)
                             ccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCa                                                                     >  2:1089473/1‑101 (MQ=255)
                              cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCAt                                                                    <  2:1498896/101‑1 (MQ=255)
                                gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCa                                                                  >  1:4004313/1‑101 (MQ=255)
                                gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCa                                                                  >  2:4043085/1‑101 (MQ=255)
                                gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCa                                                                  >  2:4712298/1‑101 (MQ=255)
                                gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCa                                                                  <  2:4836371/101‑1 (MQ=255)
                                gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCa                                                                  <  1:903465/101‑1 (MQ=255)
                                 cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCat                                                                 >  1:4085303/1‑100 (MQ=255)
                                  ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCatt                                                                <  1:4399773/101‑3 (MQ=255)
                                  ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCatt                                                                <  2:2357721/101‑3 (MQ=255)
                                   tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTc                                                               <  2:1951074/101‑1 (MQ=255)
                                    cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCt                                                              <  2:2085160/101‑1 (MQ=255)
                                    cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCt                                                              >  2:1877895/1‑101 (MQ=255)
                                      gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTg                                                            <  1:1514391/101‑1 (MQ=255)
                                        gcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.c                                                         <  1:2506478/101‑1 (MQ=255)
                                          gcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.cc                                                      >  1:978192/1‑101 (MQ=255)
                                            ggACTTCGGGGCGGCGGCGGGGCTCTTGCGAACCTGCGTTTCCGGATTGGCGACAATACCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.ccc.c                                                   >  2:4167532/1‑101 (MQ=255)
                                             gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.ccc.cc                                                  <  1:1004193/101‑2 (MQ=255)
                                             gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.ccc.cc                                                  <  1:2317200/101‑2 (MQ=255)
                                             gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.ccc.cc                                                  <  1:3613280/101‑2 (MQ=255)
                                               cTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGG.TTCCGAACCGGCATCATTCTTGG.CGCCc.cg.c                                                <  1:4569932/101‑1 (MQ=255)
                                               cTTCGGGGCGGCGGCGGGGCCCTTGCGATGCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.CGCCc.cg.c                                                >  2:2145241/1‑101 (MQ=255)
                                                ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.ccc.ccgcc                                               <  1:3218845/101‑5 (MQ=255)
                                                ggcGGGGCGGCGGCGGGGCTCTTGCGATCTTGCGTTTCCGGATTGGCGTCAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.ccc.ccgcc                                               <  2:4415277/99‑5 (MQ=255)
                                                  cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGGGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCgg                                             >  1:5020021/1‑101 (MQ=255)
                                                  cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCgg                                             >  1:755574/1‑101 (MQ=255)
                                                     ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GCTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGAc                                          <  1:5003782/101‑1 (MQ=255)
                                                      gcggcggcgGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.ccc.cctccggggca                                         >  2:2527634/1‑91 (MQ=255)
                                                         gcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATc                                      <  2:2863474/101‑1 (MQ=255)
                                                         gcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATc                                      <  2:1173766/101‑1 (MQ=255)
                                                            gcggGGCTCTTGCGATCCTGTGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACg                                   >  2:1588750/1‑101 (MQ=255)
                                                             cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACgg                                  >  2:1855093/1‑101 (MQ=255)
                                                              ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGgc                                 >  2:4284863/1‑101 (MQ=255)
                                                              ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGgc                                 >  2:3513313/1‑101 (MQ=255)
                                                              ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGgc                                 >  2:545710/1‑101 (MQ=255)
                                                              ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGgc                                 <  2:5019691/101‑1 (MQ=255)
                                                               gggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGgcc                                >  1:1268902/1‑101 (MQ=255)
                                                                    ctTGCGCTCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTc                           <  2:620111/101‑1 (MQ=255)
                                                                    ctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTc                           <  1:240424/101‑1 (MQ=255)
                                                                    ctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTc                           >  1:3812158/1‑101 (MQ=255)
                                                                     ttGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCa                          >  1:4844565/1‑101 (MQ=255)
                                                                       gCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCAtt                        <  2:1971528/101‑1 (MQ=255)
                                                                        cGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTg                       >  1:1592106/1‑101 (MQ=255)
                                                                        cGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTg                       >  2:4329392/1‑101 (MQ=255)
                                                                          aTCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGtt                     <  2:4026067/101‑1 (MQ=255)
                                                                            ccTGCGTTTCGGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTcc                   <  2:3959044/101‑1 (MQ=255)
                                                                            ccTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTcc                   <  1:1691237/101‑1 (MQ=255)
                                                                            ccTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTcc                   >  2:2572864/1‑101 (MQ=255)
                                                                                cGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCCTTGTTCCGCCa               >  2:2807040/1‑101 (MQ=255)
                                                                                cGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCa               >  1:1993757/1‑101 (MQ=255)
                                                                                 gTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  2:4068372/1‑101 (MQ=255)
                                                                                  ttgccGGATTGGCGCCAATCCCGGGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             <  1:1122557/98‑1 (MQ=255)
                                                                                   ttgcGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCCCCATTg            >  1:2885925/4‑101 (MQ=255)
                                                                                   ttCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            >  2:4897028/1‑101 (MQ=255)
                                                                                      cGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGa         >  2:1742130/1‑101 (MQ=255)
                                                                                      cGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTCG.C.CCC.CGACCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGa         >  1:1658567/1‑101 (MQ=255)
                                                                                       ggATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  1:224227/1‑101 (MQ=255)
                                                                                       ggATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  2:267249/1‑101 (MQ=255)
                                                                                       ggATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        <  1:2938201/101‑1 (MQ=255)
                                                                                        gATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       >  2:1258083/1‑101 (MQ=255)
                                                                                         aTTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      >  1:626840/1‑101 (MQ=255)
                                                                                         aTTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      <  1:1232821/101‑1 (MQ=255)
                                                                                         aTTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      <  1:3531180/101‑1 (MQ=255)
                                                                                         aTTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.C.CCCACG.CCGGGACAATCACGGCAGCCTCATTGTTCCGCCATTGCGATCa      >  1:4244323/1‑101 (MQ=255)
                                                                                          tgggCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     <  2:1335814/99‑1 (MQ=255)
                                                                                           tGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    >  2:1553451/1‑101 (MQ=255)
                                                                                           tGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    <  2:2925766/101‑1 (MQ=255)
                                                                                            ggCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAg   >  1:4874146/1‑101 (MQ=255)
                                                                                             gCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  <  1:3761281/101‑1 (MQ=255)
                                                                                             gCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  <  1:2226057/101‑1 (MQ=255)
                                                                                                     |                                                                                                
AAAATTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CCGCG.CGC.AGC.G.TTCCGAACCGGCATCAATCTTGG.C.CCC.CG.CCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057725‑2057913

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG