Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,285 +G 100% coding (92/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2851.→G100.0% 682.6 176coding (92/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (97/78):  ref base (1/0):  total (98/78)

Only 100 of 210 total aligned reads displayed.

GATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AA..GGG.TTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATC  >  NC_014034/2177191‑2177374
                                                                                                       |                                                                                             
 aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggt                                                                                            <  2:3929179/101‑3 (MQ=255)
 aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCACCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggt                                                                                            <  2:1992760/101‑3 (MQ=255)
 aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTAGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggg                                                                                            >  2:3186357/1‑99 (MQ=255)
  tCTTGCCCGCATAGGTGGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggtg                                                                                           <  1:2205646/101‑4 (MQ=255)
  tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggtg                                                                                           >  1:617903/1‑98 (MQ=255)
  tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggtg                                                                                           <  2:1970987/101‑4 (MQ=255)
   cTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggtga                                                                                          <  1:3261683/101‑5 (MQ=255)
    ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggtgat                                                                                         <  2:2257082/101‑6 (MQ=255)
     tGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggtgatc                                                                                        >  1:3499599/1‑95 (MQ=255)
      gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCTAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggtgatca                                                                                       <  1:760362/101‑8 (MQ=255)
      gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggtgatca                                                                                       >  1:2158992/1‑94 (MQ=255)
      gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggtgatca                                                                                       >  2:2088517/1‑94 (MQ=255)
      gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggtgatca                                                                                       <  1:2484563/101‑8 (MQ=255)
      gCCCCCATAGGGCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggtgatga                                                                                       <  2:439758/101‑8 (MQ=255)
        ccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggtgatcaga                                                                                     <  1:4948669/101‑10 (MQ=255)
        ccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTt.ggtgatcaga                                                                                     >  1:3874972/1‑92 (MQ=255)
           cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGAtcagacag                                                                                   >  1:1715045/1‑101 (MQ=255)
           cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGAtcagacag                                                                                   >  1:2923697/1‑101 (MQ=255)
            aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGAtcagacagc                                                                                  >  2:595896/1‑100 (MQ=255)
            aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAgg                                                                                  >  2:4495535/1‑101 (MQ=255)
             tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGc                                                                                 >  1:834365/1‑101 (MQ=255)
             tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGc                                                                                 >  1:4009420/1‑101 (MQ=255)
                gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGcccc                                                                              >  1:1260357/1‑101 (MQ=255)
                gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGcccc                                                                              >  2:4034898/1‑101 (MQ=255)
                 tCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCg                                                                             >  2:2566640/1‑101 (MQ=255)
                 tCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCg                                                                             <  2:2158844/101‑1 (MQ=255)
                  cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCgg                                                                            <  2:4545335/101‑1 (MQ=255)
                   gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AATAAG.GGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGt                                                                           >  2:5011484/1‑101 (MQ=255)
                    agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGtt                                                                          <  2:3406958/98‑1 (MQ=255)
                     gagCACCAGCCCCATCTCGGTCTCCGTTGCCGAAGCACCTTCCTTCCTCTTGAC.G.AA.AATGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGttt                                                                         <  2:1670067/99‑1 (MQ=255)
                     gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGttt                                                                         <  2:2895901/99‑1 (MQ=255)
                     gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGttt                                                                         >  1:1071213/3‑101 (MQ=255)
                      agCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTc                                                                        <  2:4937269/100‑1 (MQ=255)
                      agCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTc                                                                        >  1:1779762/2‑101 (MQ=255)
                       gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCg                                                                       >  2:2385556/1‑101 (MQ=255)
                          ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGcc                                                                    >  1:771462/1‑101 (MQ=255)
                           cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                                   <  1:3018892/101‑1 (MQ=255)
                           cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                                   >  2:2757300/1‑101 (MQ=255)
                            aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.a                                                                 >  2:3645165/1‑101 (MQ=255)
                              ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aag                                                               <  1:212637/101‑3 (MQ=255)
                               cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagc                                                              <  2:5051455/101‑4 (MQ=255)
                               cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagc                                                              >  2:3908650/1‑98 (MQ=255)
                                   tctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                                           <  2:534579/101‑1 (MQ=255)
                                    ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                          >  1:5021645/1‑101 (MQ=255)
                                      cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                                        >  1:1536848/1‑101 (MQ=255)
                                      cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                                        >  1:3244134/1‑101 (MQ=255)
                                       ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                                       >  1:454628/1‑101 (MQ=255)
                                          ggCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                    <  1:3279480/100‑1 (MQ=255)
                                          cGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                    >  1:4507688/1‑101 (MQ=255)
                                           gCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                   <  2:1189686/101‑1 (MQ=255)
                                           gCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                   >  1:2171938/1‑101 (MQ=255)
                                             cTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                                                 >  1:505273/1‑101 (MQ=255)
                                                tGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                                              >  2:2706320/1‑101 (MQ=255)
                                                tGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                                              <  2:1296231/101‑1 (MQ=255)
                                                 ttccgttctccttccttCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                                             <  1:4214853/92‑1 (MQ=255)
                                                  ccGAAGCACCTTCCTTCATCTTGACGG.AA.AAG.GGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                            <  1:1856278/101‑1 (MQ=255)
                                                  ccGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                            <  1:2086284/101‑1 (MQ=255)
                                                  ccGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCCGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                            >  1:4385442/1‑101 (MQ=255)
                                                   cGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                           >  1:3532298/1‑101 (MQ=255)
                                                   cGAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                           >  1:1225095/1‑101 (MQ=255)
                                                    gAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                          >  1:3341280/1‑101 (MQ=255)
                                                    gAAGCACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                          >  2:3733872/1‑101 (MQ=255)
                                                     aaGCACCTTCCTTCATCTTGAC.GAAA.AA..GGGATTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                         >  2:2193600/1‑101 (MQ=255)
                                                        cACCTTCCTTCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                                      >  2:564975/1‑101 (MQ=255)
                                                          ccttccttCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaagatcggaa                                    >  2:4214536/1‑93 (MQ=255)
                                                          ccttccttCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                   <  2:3047244/101‑1 (MQ=255)
                                                          ccttccttCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                   >  2:4070588/1‑101 (MQ=255)
                                                          ccttccttCATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                   >  2:700748/1‑101 (MQ=255)
                                                             tccttcATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                <  2:2607553/101‑3 (MQ=255)
                                                                ttcATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCatgtcgag                              <  2:1536718/101‑8 (MQ=255)
                                                                ttcATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                             <  1:3444858/101‑6 (MQ=255)
                                                                ttcATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                             >  2:2024208/1‑96 (MQ=255)
                                                                  cATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                            >  1:3784536/1‑101 (MQ=255)
                                                                  cATCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                            <  1:3417586/101‑1 (MQ=255)
                                                                   aTCTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                          <  1:1324462/101‑1 (MQ=255)
                                                                     cTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                        >  1:3596862/1‑101 (MQ=255)
                                                                     cTTGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                        >  1:2378771/1‑101 (MQ=255)
                                                                      ttGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cccc                       <  1:1006109/101‑1 (MQ=255)
                                                                      ttGAC.G.AA.AAAGGGG.TTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cccc                       <  2:1224978/101‑1 (MQ=255)
                                                                                    aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc              <  1:4070891/91‑1 (MQ=255)
                                                                                    aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc              <  1:4664326/91‑1 (MQ=255)
                                                                                      gtgttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAg            <  2:296046/93‑1 (MQ=255)
                                                                                       ggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t          <  2:3288098/94‑1 (MQ=255)
                                                                                       ggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t          <  1:3838946/94‑1 (MQ=255)
                                                                                       ggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t          >  2:4707261/8‑101 (MQ=255)
                                                                                        gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt         <  1:3677882/95‑1 (MQ=255)
                                                                                        gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt         >  2:3616727/7‑101 (MQ=255)
                                                                                          tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg       >  1:4743565/5‑99 (MQ=255)
                                                                                          tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg       <  1:157126/97‑3 (MQ=255)
                                                                                          tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg       >  2:3213782/5‑99 (MQ=255)
                                                                                           ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc      >  1:948329/4‑98 (MQ=255)
                                                                                           ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc      <  1:2664681/98‑4 (MQ=255)
                                                                                             tgtcggtcTTGGTGATCAGGCAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc    <  2:4349250/100‑6 (MQ=255)
                                                                                             tggcggtcTTGGTGATCAGGCAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc    <  2:2241578/98‑6 (MQ=255)
                                                                                              gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca    >  2:4915169/1‑101 (MQ=255)
                                                                                                cggtcTTGGTGATCAGACAGGCCCGGGTTTCGGCGGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc  <  1:4798892/101‑1 (MQ=255)
                                                                                                cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGGAGAGGCCCCCAACAGGGCCAGTTTGCGcatc  <  1:4527696/101‑1 (MQ=255)
                                                                                                cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc  <  2:8220/101‑1 (MQ=255)
                                                                                                cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc  <  2:3969110/101‑1 (MQ=255)
                                                                                                cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc  >  1:908168/1‑101 (MQ=255)
                                                                                                       |                                                                                             
GATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.G.AA.AA..GGG.TTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATC  >  NC_014034/2177191‑2177374

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG