Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,313 +A 100% coding (64/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3131.→A100.0% 772.9 195coding (64/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (105/90):  ref base (0/0):  total (105/90)

Only 100 of 214 total aligned reads displayed.

CAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCG  >  NC_014034/2177218‑2177404
                                                                                                      |                                                                                              
cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                                                                 >  2:2757300/1‑101 (MQ=255)
cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                                                                 <  1:2150077/101‑1 (MQ=255)
 aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.a                                                                                               <  2:2238379/101‑1 (MQ=255)
 aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.a                                                                                               >  2:4975110/1‑101 (MQ=255)
  gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCATACAGGCCCCGGTTTCGGCCG.aa                                                                                              <  2:2935674/101‑2 (MQ=255)
   ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aag                                                                                             <  1:3806708/101‑3 (MQ=255)
   ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aag                                                                                             >  2:2036406/1‑99 (MQ=255)
    cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagc                                                                                            >  2:3908650/1‑98 (MQ=255)
       aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgc                                                                                          >  1:1542707/1‑101 (MQ=255)
         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                                                        >  1:5021645/1‑101 (MQ=255)
          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                                                                       <  2:587671/101‑1 (MQ=255)
           cGGTCTCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                                                                      <  1:3255521/101‑1 (MQ=255)
            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                                                                     >  1:4437125/1‑101 (MQ=255)
            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                                                                     >  1:224812/1‑101 (MQ=255)
              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                                                   >  1:4559438/1‑101 (MQ=255)
               ggCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                                                  <  1:3279480/100‑1 (MQ=255)
                gCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                                                 <  2:1189686/101‑1 (MQ=255)
                 ccTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                                                                                >  2:3651017/1‑101 (MQ=255)
                  cTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                                                                               >  1:505273/1‑101 (MQ=255)
                     tGCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                                                                            >  2:2706320/1‑101 (MQ=255)
                      ttccgttctccttccttCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                                                                           <  1:4214853/92‑1 (MQ=255)
                      gCCGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                                                                           >  1:4526864/1‑101 (MQ=255)
                       ccGAAGCACCTTCCTTCATCTTGACGGAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                                                          <  1:1856278/101‑1 (MQ=255)
                       ccGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                                                          <  1:1366182/101‑1 (MQ=255)
                        cGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                                                         >  2:556593/1‑101 (MQ=255)
                        cGAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                                                         >  1:3532298/1‑101 (MQ=255)
                         gAAGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                                                        >  2:126987/1‑101 (MQ=255)
                          aaGCACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                                                       <  2:4067804/101‑1 (MQ=255)
                             cACCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                                                                    >  1:3491747/1‑101 (MQ=255)
                              aCCTTCCTTCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                   >  1:1988884/1‑101 (MQ=255)
                               ccttccttCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                                                 <  2:3047244/101‑1 (MQ=255)
                               ccttccttCATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                                                 >  2:700748/1‑101 (MQ=255)
                                cttccttcATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                >  2:3743909/1‑101 (MQ=255)
                                cttccttcATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                >  1:873378/1‑101 (MQ=255)
                                  tccttcATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                              <  2:2607553/101‑3 (MQ=255)
                                    cttcATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                                                            <  1:610275/101‑5 (MQ=255)
                                    cttcATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                                                            <  1:1299788/101‑5 (MQ=255)
                                     ttcATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                           <  1:3060301/101‑6 (MQ=255)
                                      tcATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                           <  1:3359659/101‑1 (MQ=255)
                                      tcATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                           >  2:2697683/1‑101 (MQ=255)
                                       cATCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                          <  2:4559117/101‑1 (MQ=255)
                                         tCTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                                                       >  2:1999443/1‑101 (MQ=255)
                                          cTTGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                                                      >  1:1163358/1‑101 (MQ=255)
                                           ttGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cccc                                                     >  1:24044/1‑101 (MQ=255)
                                           ttGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cccc                                                     >  1:3420267/1‑101 (MQ=255)
                                           ttGAC.GAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cccc                                                     <  1:1006109/101‑1 (MQ=255)
                                                      aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                                            <  1:4664326/91‑1 (MQ=255)
                                                      aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                                            >  1:4536016/11‑101 (MQ=255)
                                                      aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                                            <  1:4070891/91‑1 (MQ=255)
                                                      aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                                            <  2:53052/91‑1 (MQ=255)
                                                      aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                                            <  1:3322149/91‑1 (MQ=255)
                                                       ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa                                           <  2:4391959/92‑1 (MQ=255)
                                                         ggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGGCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t                                        >  2:1656428/8‑101 (MQ=255)
                                                         ggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t                                        >  2:4707261/8‑101 (MQ=255)
                                                          gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt                                       <  1:3677882/95‑1 (MQ=255)
                                                          gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt                                       >  2:3616727/7‑101 (MQ=255)
                                                          gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt                                       <  2:2171783/95‑1 (MQ=255)
                                                           ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.ttt                                      >  1:1226707/6‑100 (MQ=255)
                                                            tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                     <  1:157126/97‑3 (MQ=255)
                                                            tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                     >  2:2969987/5‑99 (MQ=255)
                                                            tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                     >  2:3213782/5‑99 (MQ=255)
                                                            tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                     >  1:4743565/5‑99 (MQ=255)
                                                             ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                    <  1:2664681/98‑4 (MQ=255)
                                                               tgtcggtcTTGGTGATCAGGCAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc                                  <  2:4349250/100‑6 (MQ=255)
                                                                gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca                                  >  2:4915169/1‑101 (MQ=255)
                                                                  cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                >  1:908168/1‑101 (MQ=255)
                                                                  cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                <  2:3969110/101‑1 (MQ=255)
                                                                     tcttggtgatcagacagNCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCatcatc                             <  2:191408/91‑1 (MQ=255)
                                                                     tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCatcatc                             >  2:4188067/11‑101 (MQ=255)
                                                                     tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCatcatc                             >  2:1055809/11‑101 (MQ=255)
                                                                      cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                            >  1:2498577/10‑101 (MQ=255)
                                                                      cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                            >  2:4156757/10‑101 (MQ=255)
                                                                       ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                           <  2:1736620/93‑1 (MQ=255)
                                                                        tggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                          >  1:2329923/8‑101 (MQ=255)
                                                                         ggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACgg                         <  1:2949456/95‑1 (MQ=255)
                                                                         ggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACgg                         <  1:1786950/95‑1 (MQ=255)
                                                                         ggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACgg                         >  1:4572155/7‑101 (MQ=255)
                                                                          gtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGt                        <  2:2057180/96‑1 (MQ=255)
                                                                          gtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGt                        <  1:4972210/96‑1 (MQ=255)
                                                                             atcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctc                     <  2:908063/99‑1 (MQ=255)
                                                                             atcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctc                     >  1:20890/3‑101 (MQ=255)
                                                                             atcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctc                     <  2:4933205/99‑1 (MQ=255)
                                                                             atcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctc                     >  2:3484020/3‑101 (MQ=255)
                                                                               cagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctctc                   <  1:1899852/101‑1 (MQ=255)
                                                                               cagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctctc                   <  2:1409475/101‑1 (MQ=255)
                                                                                 gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcct                 >  2:1493656/1‑101 (MQ=255)
                                                                                   cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc               <  2:803443/101‑1 (MQ=255)
                                                                                    agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc              >  2:3791041/1‑101 (MQ=255)
                                                                                     ggCCCCGGTTTCGGCCGAAGCAGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCg             >  1:2877412/1‑101 (MQ=255)
                                                                                      gCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGt            <  1:2193755/101‑1 (MQ=255)
                                                                                        cccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGttt          <  1:4908281/101‑1 (MQ=255)
                                                                                        cccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGttt          <  2:224778/101‑1 (MQ=255)
                                                                                         ccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc         <  2:45288/101‑1 (MQ=255)
                                                                                         ccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc         <  2:4880428/101‑1 (MQ=255)
                                                                                            gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt      >  2:4221811/1‑101 (MQ=255)
                                                                                             tttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg     >  1:1318210/1‑101 (MQ=255)
                                                                                              ttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt    >  1:3766439/1‑101 (MQ=255)
                                                                                               tCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc   >  2:972560/1‑101 (MQ=255)
                                                                                                cGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg  <  1:2036550/101‑1 (MQ=255)
                                                                                                cGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg  <  2:2296295/101‑1 (MQ=255)
                                                                                                      |                                                                                              
CAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAC.GAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCG  >  NC_014034/2177218‑2177404

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG