Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,186,198 +G 100% intergenic (+471/‑34) mtlK → / → RCAP_rcc02028 mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,186,1981.→G100.0% 724.2 191intergenic (+471/‑34)mtlK/RCAP_rcc02028mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (96/95):  ref base (0/0):  total (96/95)

Only 100 of 220 total aligned reads displayed.

CCGGGTTGGGAATGATCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGC.GGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCATTCCGCCAGCCTGC  >  NC_014034/2186098‑2186295
                                                                                                     |                                                                                                  
ccGGGTTGGGAATGATCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCcggc                                                                                                     >  1:1544770/1‑101 (MQ=255)
 cGGGTTGGGAATGATCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCcggc.g                                                                                                   >  1:2757917/1‑101 (MQ=255)
 cGGGTTGGGAATGATCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCcggc.g                                                                                                   >  1:2601872/1‑101 (MQ=255)
  gggTTGGGAATGATCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCcggc.gg                                                                                                  >  2:4167201/1‑101 (MQ=255)
      tGGGAATGATCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGC.ggggcc                                                                                              >  2:4001621/1‑98 (MQ=255)
         gAATGATCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCt                                                                                           <  1:3769897/101‑1 (MQ=255)
          aaTGATCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTg                                                                                          >  2:4721339/1‑101 (MQ=255)
           aTGATCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTgg                                                                                         <  1:314272/101‑1 (MQ=255)
            tGATCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGc                                                                                        <  2:3328882/101‑1 (MQ=255)
            tGATCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGc                                                                                        >  2:1350839/1‑101 (MQ=255)
             gATCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGcc                                                                                       <  2:4759619/101‑1 (MQ=255)
              aTCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCg                                                                                      <  1:262311/101‑1 (MQ=255)
              aTCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCCGGccc                                                                                      >  2:3489751/1‑100 (MQ=255)
                cGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAg                                                                                    <  1:3468353/101‑1 (MQ=255)
                 gCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGc                                                                                   <  2:2601691/101‑1 (MQ=255)
                 gCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGc                                                                                   <  2:2784414/101‑1 (MQ=255)
                 gCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGc                                                                                   >  1:3647233/1‑101 (MQ=255)
                  cccGGCGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCa                                                                                  <  2:2252697/101‑1 (MQ=255)
                   ccGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCAt                                                                                 >  1:2441379/1‑101 (MQ=255)
                     gACGCCGGGCGGGATCTTCCCCGCGCAGCCGCAAGGGCCGCACCGGTGCGGAACCCGTCAGAACTCCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCg                                                                               <  2:1682814/101‑1 (MQ=255)
                         ccAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.GCGCTGGCCGAGCATCGGCCg                                                                           >  1:2207541/1‑101 (MQ=255)
                          cAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGc                                                                          <  1:904136/101‑1 (MQ=255)
                          cAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGc                                                                          >  2:3568381/1‑101 (MQ=255)
                            ggCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGCCGATTGGCCGCtc                                                                        >  1:978951/1‑100 (MQ=255)
                            ggCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCtt                                                                        >  2:4224082/1‑101 (MQ=255)
                            ggCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCtt                                                                        <  2:4738772/101‑1 (MQ=255)
                             gCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTg                                                                       <  1:3607673/101‑1 (MQ=255)
                               gggATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTggg                                                                     <  2:1134627/101‑1 (MQ=255)
                               gggATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTggg                                                                     >  2:2551439/1‑101 (MQ=255)
                                ggATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGc                                                                    >  1:50644/1‑101 (MQ=255)
                                  aTCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAg                                                                  >  1:2000061/1‑101 (MQ=255)
                                    cTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGAt                                                                >  2:2703689/1‑101 (MQ=255)
                                    cTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGAt                                                                >  1:4612220/1‑101 (MQ=255)
                                    cTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGAt                                                                >  1:3842085/1‑101 (MQ=255)
                                    cTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGAt                                                                >  1:2021756/1‑101 (MQ=255)
                                     ttCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATg                                                               >  1:1243554/1‑101 (MQ=255)
                                      tCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGc                                                              >  2:3295681/1‑101 (MQ=255)
                                         cGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGcaca                                                           <  1:2657408/101‑1 (MQ=255)
                                           aCTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAg                                                         >  1:3081692/1‑101 (MQ=255)
                                              cAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGc                                                      <  1:2856402/101‑1 (MQ=255)
                                                gCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTa                                                    <  1:4167518/101‑1 (MQ=255)
                                                gCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTa                                                    <  1:3295943/101‑1 (MQ=255)
                                                gCCGCAAGGACCGCAGCGATGTTGAACCAGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTa                                                    <  1:4896849/101‑1 (MQ=255)
                                                 ccGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTAt                                                   <  1:4030911/101‑1 (MQ=255)
                                                 ccGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTAt                                                   <  1:727401/101‑1 (MQ=255)
                                                  cGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTAtt                                                  >  2:3683303/1‑101 (MQ=255)
                                                  cGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTAtt                                                  <  1:3823161/101‑1 (MQ=255)
                                                  cGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTAtt                                                  >  1:4739248/1‑101 (MQ=255)
                                                  cGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTAtt                                                  >  2:2194813/1‑101 (MQ=255)
                                                    cAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGCGCATCGGCCGCTTGGGCAGAAGCACCAGGGCTATTCg                                                >  1:4242309/1‑101 (MQ=255)
                                                    cAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCg                                                >  2:1784506/1‑101 (MQ=255)
                                                      aGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCggg                                              >  1:3745568/1‑101 (MQ=255)
                                                        gACCGCTGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGcc                                            <  1:828119/101‑1 (MQ=255)
                                                        gACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGcc                                            <  1:4755467/101‑1 (MQ=255)
                                                         aCCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCa                                           >  1:2223190/1‑101 (MQ=255)
                                                          ccGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCAt                                          <  1:3961984/101‑1 (MQ=255)
                                                          ccGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCAt                                          <  1:2138692/101‑1 (MQ=255)
                                                          ccGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCAt                                          <  2:77368/101‑1 (MQ=255)
                                                           cGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATg                                         <  1:4809873/101‑1 (MQ=255)
                                                           cGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATg                                         >  1:1396641/1‑101 (MQ=255)
                                                             cAGCGATGTTGACCCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTc                                       <  2:4948078/101‑1 (MQ=255)
                                                              aGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCt                                      <  1:3050912/101‑1 (MQ=255)
                                                                 gATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGAGGCACAAGGGCTATTCGGGCCATGTCTTcc                                   >  1:172626/1‑101 (MQ=255)
                                                                    gTTGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCcaca                                >  1:3312265/1‑101 (MQ=255)
                                                                     ttGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTCTTCGGGCCATGTCTTCCACAt                               <  1:2871483/101‑1 (MQ=255)
                                                                     ttGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACAt                               <  2:2312305/101‑1 (MQ=255)
                                                                     ttGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACAt                               <  2:1544640/101‑1 (MQ=255)
                                                                     ttGAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCAAAAGGGCTATTCGGGCCATGTCTTCCCCAt                               >  2:822924/1‑101 (MQ=255)
                                                                       gAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATcg                             <  2:2009182/101‑1 (MQ=255)
                                                                       gAACCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATcg                             <  1:3683731/101‑1 (MQ=255)
                                                                         aCCCGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATcgcc                           <  1:2194968/101‑1 (MQ=255)
                                                                           ccGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATcgccgc                         <  2:4492577/101‑1 (MQ=255)
                                                                           ccGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATcgccgc                         <  2:706115/101‑1 (MQ=255)
                                                                            cGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATcgccgcc                        <  2:2991591/101‑1 (MQ=255)
                                                                            cGTCCGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATcgccgcc                        <  2:2441205/101‑1 (MQ=255)
                                                                               ccGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATcgccgccgcc                     >  2:407307/1‑101 (MQ=255)
                                                                                cGAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATcgccgccgccg                    <  1:149684/101‑1 (MQ=255)
                                                                                 gAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATcgccgccgccgc                   >  2:2084899/1‑101 (MQ=255)
                                                                                 gAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATcgccgccgccgc                   >  1:4470858/1‑101 (MQ=255)
                                                                                 gAACTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATcgccgccgccgc                   <  2:2846079/101‑1 (MQ=255)
                                                                                  aaCTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCcgcg                  >  1:665165/1‑101 (MQ=255)
                                                                                  aaCTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCcgcg                  >  1:3172888/1‑101 (MQ=255)
                                                                                   aCTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCgcgc                 <  2:3841800/101‑1 (MQ=255)
                                                                                    cTTCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCa                >  1:936402/1‑101 (MQ=255)
                                                                                     ttCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCAt               <  1:39439/101‑1 (MQ=255)
                                                                                      tCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCAtt              >  1:4291504/1‑101 (MQ=255)
                                                                                      tCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCAtt              <  1:1903136/101‑1 (MQ=255)
                                                                                      tCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCAtt              >  2:2642143/1‑101 (MQ=255)
                                                                                      tCCGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCAtt              >  2:529361/1‑101 (MQ=255)
                                                                                        cGCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCATTcc            <  2:3595702/101‑1 (MQ=255)
                                                                                        cGCAGCGCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCATTcc            <  1:2412833/101‑1 (MQ=255)
                                                                                         gCAGCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCATTCCg           >  1:4328370/1‑101 (MQ=255)
                                                                                          cAGCTCCCGGCGGGG.CAGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCATTCCGc          >  2:380216/1‑101 (MQ=255)
                                                                                            gCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCATTCCGCCa        <  2:3241400/101‑1 (MQ=255)
                                                                                            gCTCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGATATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCATTCCGCCa        <  1:1165957/101‑1 (MQ=255)
                                                                                              tCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCATTCCGCCAGc      <  1:1056840/101‑1 (MQ=255)
                                                                                              tCCCGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGACATGTCTTCCACATCGCCGCCGCCGCGCATTCCGCCAGc      >  1:1985892/1‑101 (MQ=255)
                                                                                               cccGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACCAGGGCTATTTGGGCCATGTCTTCCACATCGCCGCCGCCGCGCCTTCCGCCAGcc     >  2:4349311/1‑101 (MQ=255)
                                                                                                ccGGCGGGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCATTCCGCCAGCCt    >  2:3027501/1‑101 (MQ=255)
                                                                                                  ggc.ggGCCCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCATTCCGCCAGCCTGc  <  2:1017665/101‑1 (MQ=255)
                                                                                                     |                                                                                                  
CCGGGTTGGGAATGATCGCCCGACGCCAGGCGGGATCTTCCCGACTCAGCCGCAAGGACCGCAGCGATGTTGAACCCGTCCGAACTTCCGCAGCTCCCGGC.GGG.CCGCTGGCCGAGCATCGGCCGCTTGGGCAGATGCACAAGGGCTATTCGGGCCATGTCTTCCACATCGCCGCCGCCGCGCATTCCGCCAGCCTGC  >  NC_014034/2186098‑2186295

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG