Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,280 +C 100% intergenic (‑76/‑229) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,2801.→C100.0% 915.6 242intergenic (‑76/‑229)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (109/131):  ref base (2/0):  total (111/131)

Only 100 of 285 total aligned reads displayed.

TCGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCC  >  NC_014034/2249180‑2249377
                                                                                                     |                                                                                                  
tCGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGCGGGCTCTg                                                                                                     >  1:4781582/1‑101 (MQ=255)
 cGAGGACAGAAGATCGCCGGACATGGGGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.c                                                                                                   >  2:2014524/1‑101 (MQ=255)
  gAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc                                                                                                  >  1:2863953/1‑101 (MQ=255)
  gAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc                                                                                                  >  1:1009919/1‑101 (MQ=255)
   aGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc                                                                                                 >  2:473382/1‑101 (MQ=255)
   aGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc                                                                                                 <  1:2254759/101‑1 (MQ=255)
     gACAGAAGATCGCCGGACATGGTGCTGGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc.cc                                                                                              <  1:4239566/101‑2 (MQ=255)
       cAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc.ccgt                                                                                            <  2:3782821/101‑4 (MQ=255)
         gaagaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc.ccgtcc                                                                                          <  2:3150108/101‑6 (MQ=255)
         gaagaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc.ccgtcc                                                                                          >  1:2584903/1‑96 (MQ=255)
         gaagaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc.ccgtcc                                                                                          <  2:1929789/101‑6 (MQ=255)
          aagaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGNGGCTCTGCCcc.cgtccc                                                                                          >  2:1092626/1‑101 (MQ=255)
          aagaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCcc.cgtccc                                                                                          >  2:2943752/1‑101 (MQ=255)
           agaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCcc.cgtcccg                                                                                         >  1:1152161/1‑101 (MQ=255)
             aTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTg                                                                                       >  2:2181073/1‑101 (MQ=255)
             aTCGCCGGACATGGTGCTCGCTGTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTg                                                                                       <  2:4231853/101‑1 (MQ=255)
              tCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGc                                                                                      >  2:2590878/1‑101 (MQ=255)
               cGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGcc                                                                                     <  2:1347130/101‑1 (MQ=255)
               cGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGcc                                                                                     >  1:1345093/1‑101 (MQ=255)
               cGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGcc                                                                                     >  1:4118423/1‑101 (MQ=255)
                gCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCg                                                                                    <  1:4710840/101‑1 (MQ=255)
                  cGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCggg                                                                                  <  1:5045011/101‑1 (MQ=255)
                  cGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCggg                                                                                  >  1:4968108/1‑101 (MQ=255)
                     aCATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACt                                                                               >  1:2779011/1‑101 (MQ=255)
                     aCATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACt                                                                               <  1:3601057/101‑1 (MQ=255)
                      cATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTc                                                                              <  2:4886358/101‑1 (MQ=255)
                       aTGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACtac                                                                             >  2:3679211/1‑99 (MQ=255)
                         ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTcccc                                                                           <  1:4381506/101‑1 (MQ=255)
                         ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTcccc                                                                           >  2:127350/1‑101 (MQ=255)
                         ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTcccc                                                                           >  1:608299/1‑101 (MQ=255)
                          gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTccccc                                                                          >  1:4164193/1‑101 (MQ=255)
                          gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTccccc                                                                          >  1:2474973/1‑101 (MQ=255)
                           tGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCg                                                                         >  2:3311870/1‑101 (MQ=255)
                              tCGCTTTCATTGGTGTTCTTGAGCTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGa                                                                      <  1:668911/101‑1 (MQ=255)
                               cGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGat                                                                     <  1:4207857/101‑1 (MQ=255)
                                gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGata                                                                    >  1:3695495/1‑101 (MQ=255)
                                gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGata                                                                    >  2:4499140/1‑101 (MQ=255)
                                 gtttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGAAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGatat                                                                   <  2:2863740/100‑1 (MQ=255)
                                  tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATAtt                                                                  >  1:2188428/1‑101 (MQ=255)
                                  tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATAtt                                                                  >  1:270985/1‑101 (MQ=255)
                                   ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATAttt                                                                 >  1:875843/1‑101 (MQ=255)
                                   ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATAttt                                                                 <  2:616944/101‑1 (MQ=255)
                                   ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATAttt                                                                 >  2:2728375/1‑101 (MQ=255)
                                    tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTg                                                                <  2:2260955/101‑1 (MQ=255)
                                     cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGa                                                               <  2:3552821/101‑1 (MQ=255)
                                     cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGa                                                               >  1:1789917/1‑101 (MQ=255)
                                     cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGa                                                               <  1:3882108/101‑1 (MQ=255)
                                       ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAgg                                                             <  2:1505130/101‑1 (MQ=255)
                                       ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAgg                                                             >  2:363864/1‑101 (MQ=255)
                                       ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAgg                                                             <  2:56748/101‑1 (MQ=255)
                                       ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAgg                                                             <  2:321150/101‑1 (MQ=255)
                                        tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGc                                                            >  1:1391973/1‑101 (MQ=255)
                                         ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCa                                                           <  1:4453268/101‑1 (MQ=255)
                                          gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCaa                                                          >  1:2653259/1‑101 (MQ=255)
                                           tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAg                                                         >  1:157023/1‑101 (MQ=255)
                                              tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTTAGGCAAGATg                                                      <  2:4976744/101‑1 (MQ=255)
                                              tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATg                                                      >  2:4419981/1‑101 (MQ=255)
                                                  gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgg                                                  >  1:194776/1‑101 (MQ=255)
                                                     aTGCACAGATACCGCAGGTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAggggg                                               <  2:4536363/101‑1 (MQ=255)
                                                     aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAggggg                                               <  2:3218531/101‑1 (MQ=255)
                                                       gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGc                                             >  2:4040796/1‑101 (MQ=255)
                                                       gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGc                                             >  1:4285539/1‑101 (MQ=255)
                                                         acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCtt                                           >  1:2427350/1‑101 (MQ=255)
                                                          caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCttt                                          <  1:2547202/101‑1 (MQ=255)
                                                          caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCttt                                          <  1:4517889/101‑1 (MQ=255)
                                                          caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCttt                                          <  1:2943980/101‑1 (MQ=255)
                                                           aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTc                                         <  1:998889/101‑1 (MQ=255)
                                                           aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTc                                         <  1:725887/101‑1 (MQ=255)
                                                            gATACCGCAGCTTGGGCNCCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCa                                        <  2:2347573/101‑1 (MQ=255)
                                                            gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCa                                        <  1:3017652/101‑1 (MQ=255)
                                                             aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCAt                                       <  1:3172710/101‑1 (MQ=255)
                                                              tcccGCAGCTTGGGCCCCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATc                                      <  2:399346/99‑1 (MQ=255)
                                                               aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCt                                     >  2:4356992/1‑101 (MQ=255)
                                                               aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCt                                     <  2:3236294/101‑1 (MQ=255)
                                                               aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCCTTCGTCt                                     >  1:1260017/1‑101 (MQ=38)
                                                                  gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGc                                  >  1:3509049/1‑101 (MQ=255)
                                                                    aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCg                                <  1:1832923/101‑1 (MQ=255)
                                                                     ggttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGa                               >  1:3356129/3‑101 (MQ=255)
                                                                     gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGa                               >  1:1296416/1‑101 (MQ=255)
                                                                       ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaaa                             >  2:1220639/1‑101 (MQ=255)
                                                                       ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaaa                             >  1:16496/1‑101 (MQ=255)
                                                                        tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaaaa                            <  2:4579861/101‑1 (MQ=255)
                                                                         gggCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAat                           >  1:3198249/1‑101 (MQ=255)
                                                                             aCCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATcc                       <  2:157007/101‑1 (MQ=255)
                                                                              cccGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATccc                      >  2:3515121/1‑101 (MQ=255)
                                                                                      aagaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGGTTTCATCTTGCCGAAAATATCCCGGGGGGTg              >  1:3824074/1‑101 (MQ=255)
                                                                                      aagaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg              >  1:308160/1‑101 (MQ=255)
                                                                                      aagaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg              <  1:4613386/101‑1 (MQ=255)
                                                                                      aagaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg              <  1:4026597/101‑1 (MQ=255)
                                                                                      aagaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg              >  2:4681391/1‑101 (MQ=255)
                                                                                      aagaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg              >  2:4806118/1‑101 (MQ=255)
                                                                                        gaagGGGGCTCTGCCCC.CGTCCCGTGCCTGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCTAAAATATCCCGGGGGGTGaa            <  1:4724646/101‑1 (MQ=37)
                                                                                        gaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGTAAATATCCCGGGGGGTGaa            >  1:329222/1‑101 (MQ=255)
                                                                                         aagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt           <  2:3508779/101‑1 (MQ=255)
                                                                                         aagGGGGCTCTG.CCCACGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt           >  2:3903940/1‑101 (MQ=255)
                                                                                         aagGCGGCTCGGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt           <  2:673696/101‑1 (MQ=38)
                                                                                           gggggCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATgg         >  1:3951012/1‑101 (MQ=255)
                                                                                            ggggCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc        >  1:1053981/1‑101 (MQ=255)
                                                                                              ggCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAATGgcgc      >  1:1234036/1‑101 (MQ=255)
                                                                                                  ctG.CCCTCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc  <  2:2212381/101‑1 (MQ=255)
                                                                                                     |                                                                                                  
TCGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCC  >  NC_014034/2249180‑2249377

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG