Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,300 +C 3.4% intergenic (‑96/‑209) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3001.→C 3.4% 19.2 ‑0.3 ‑0.4262intergenic (‑96/‑209)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (5/4):  ref base (123/130):  total (128/134)
Fisher's exact test strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.28e-01

Only 100 of 299 total aligned reads displayed.

ACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCTTCG  >  NC_014034/2249201‑2249400
                                                                                                     |                                                                                                       
aCATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACt                                                                                                          <  1:1469601/101‑1 (MQ=255)
 cATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.c                                                                                                        <  2:4886358/101‑1 (MQ=255)
     gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.ccccc                                                                                                    >  1:4164193/1‑101 (MQ=255)
       gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCgg                                                                                                  >  1:88673/1‑101 (MQ=255)
       gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCgg                                                                                                  >  1:540533/1‑101 (MQ=255)
        cTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCggg                                                                                                 >  2:3881816/1‑101 (MQ=255)
          cGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGat                                                                                               <  1:4207857/101‑1 (MQ=255)
            cTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGatat                                                                                             <  2:865180/101‑1 (MQ=255)
             tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATAtt                                                                                            >  1:270985/1‑101 (MQ=255)
              ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATAttt                                                                                           <  2:2979931/101‑1 (MQ=255)
              ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATAttt                                                                                           <  1:220899/101‑1 (MQ=255)
              ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATAttt                                                                                           <  2:616944/101‑1 (MQ=255)
               tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTg                                                                                          <  1:3067869/101‑1 (MQ=255)
               tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTg                                                                                          <  1:2464019/101‑1 (MQ=255)
                cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGa                                                                                         <  2:3552821/101‑1 (MQ=255)
                cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGa                                                                                         <  1:3882108/101‑1 (MQ=255)
                 aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                        <  2:3695217/101‑1 (MQ=255)
                 aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                        >  1:1306822/1‑101 (MQ=255)
                  ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                       <  2:1505130/101‑1 (MQ=255)
                  ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                       <  2:321150/101‑1 (MQ=255)
                    ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                     >  1:3690304/1‑101 (MQ=255)
                    ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                     <  2:2862014/101‑1 (MQ=255)
                    ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                     <  2:3807066/101‑1 (MQ=255)
                    ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                     <  1:4453268/101‑1 (MQ=255)
                     gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCCCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCaa                                                                                    <  1:2957079/101‑1 (MQ=255)
                      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAg                                                                                   <  1:2626407/101‑1 (MQ=255)
                      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAg                                                                                   <  2:2592306/101‑1 (MQ=255)
                       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                  >  1:3585906/1‑101 (MQ=255)
                       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                  >  1:4682471/1‑101 (MQ=255)
                       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                  <  2:1152044/101‑1 (MQ=255)
                         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTTAGGCAAGATg                                                                                <  2:4976744/101‑1 (MQ=255)
                          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                               >  1:3809152/1‑101 (MQ=255)
                            tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...g                                                                          >  1:1645962/1‑101 (MQ=255)
                                  gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGc                                                                    >  2:4040796/1‑101 (MQ=255)
                                  gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGc                                                                    <  2:4448752/101‑1 (MQ=255)
                                   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCt                                                                   <  2:1330604/101‑1 (MQ=255)
                                    acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCtt                                                                  >  1:2427350/1‑101 (MQ=255)
                                    acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCtt                                                                  <  1:3126344/101‑1 (MQ=255)
                                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                 <  1:3219229/101‑1 (MQ=255)
                                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                 <  2:1075587/101‑1 (MQ=255)
                                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                 <  1:4517889/101‑1 (MQ=255)
                                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                >  2:1270258/1‑101 (MQ=255)
                                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                <  1:998889/101‑1 (MQ=255)
                                       gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.TCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCa                                                               <  2:2222766/101‑1 (MQ=255)
                                       gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCa                                                               >  1:3914683/1‑101 (MQ=255)
                                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCAt                                                              >  2:4133258/1‑101 (MQ=255)
                                         tcccGCAGCTTGGGCCCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATc                                                             <  2:399346/99‑1 (MQ=255)
                                           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                           <  1:1092739/101‑1 (MQ=255)
                                           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                           <  1:473447/101‑1 (MQ=255)
                                             gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGGTATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGc                                                         >  1:2113489/1‑101 (MQ=255)
                                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCg                                                       <  1:660105/101‑1 (MQ=255)
                                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCg                                                       <  1:1832923/101‑1 (MQ=255)
                                                ggttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGa                                                      >  1:3356129/3‑101 (MQ=255)
                                                gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGa                                                      <  2:4576435/101‑1 (MQ=255)
                                                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGCTATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                     >  1:1273009/1‑101 (MQ=255)
                                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCATGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                  <  1:4425801/101‑1 (MQ=255)
                                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                  <  2:322504/101‑1 (MQ=255)
                                                     ggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAata                                                 <  1:2177755/101‑1 (MQ=255)
                                                        aCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcc                                              <  1:431164/101‑1 (MQ=255)
                                                         cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                             >  2:3515121/1‑101 (MQ=255)
                                                         cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                             <  1:3188297/101‑1 (MQ=255)
                                                             gggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggg                                         >  2:4510651/1‑101 (MQ=255)
                                                               gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                       >  2:58101/1‑101 (MQ=255)
                                                               gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                       <  1:1759161/101‑1 (MQ=255)
                                                               gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                       <  1:2842509/101‑1 (MQ=255)
                                                                cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                      <  2:4790983/101‑1 (MQ=255)
                                                                 aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                     >  2:365312/1‑101 (MQ=255)
                                                                 aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                     >  2:4681391/1‑101 (MQ=255)
                                                                 aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                     <  1:4613386/101‑1 (MQ=255)
                                                                 aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATAATTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                     >  2:4368196/1‑101 (MQ=255)
                                                                   gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGTAAATATCCCGGGGGGTGaa                                   >  1:329222/1‑101 (MQ=255)
                                                                    aagGGGGCTCTGCCCCCGTGCCGTGCGGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                  <  2:1997524/101‑1 (MQ=37)
                                                                    aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                  <  2:3508779/101‑1 (MQ=255)
                                                                     agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                 >  1:25854/1‑101 (MQ=255)
                                                                     agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                 <  1:4281775/101‑1 (MQ=255)
                                                                       ggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                               <  2:2188273/101‑1 (MQ=255)
                                                                        gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcg                              <  1:255547/101‑1 (MQ=255)
                                                                         ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAATGgcgc                             >  1:1234036/1‑101 (MQ=255)
                                                                            tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGc                          <  2:2262889/96‑1 (MQ=255)
                                                                            tctgcccccGTCCCGTGCCGCGACT.CTCCCGGGATATATGAAGCAAGAAGAA...GGGGGCCTTACATCTTGCCGAAAATATCCCGGGGCGTGAATGGCGCAGc                          <  2:5020550/96‑1 (MQ=25)
                                                                               gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                       <  1:1942502/99‑1 (MQ=255)
                                                                               gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGAAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATACCGGGGGGGGAAGGGCGCAGCCag                       >  1:2491384/3‑101 (MQ=38)
                                                                                cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCaga                      <  1:2904333/100‑1 (MQ=255)
                                                                                 ccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCagag                     >  1:817140/1‑101 (MQ=255)
                                                                                    cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggg                  >  1:2776911/1‑101 (MQ=255)
                                                                                     gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAggggg                 >  1:4881073/1‑101 (MQ=255)
                                                                                     gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGAGAATGGCGCAGCCAGAggggg                 >  2:1813618/1‑101 (MQ=255)
                                                                                      tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggggg                >  2:4829050/1‑101 (MQ=255)
                                                                                        ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa              <  2:3319237/101‑1 (MQ=255)
                                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg             <  2:4307761/101‑1 (MQ=255)
                                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg             <  1:675225/101‑1 (MQ=255)
                                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg             >  1:396248/1‑101 (MQ=255)
                                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg             <  1:3498506/101‑1 (MQ=255)
                                                                                         cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAggggg                 >  2:2381758/8‑101 (MQ=18)
                                                                                            gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcgc          >  2:2661997/1‑101 (MQ=255)
                                                                                              gcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGCAgc            <  1:2286656/99‑1 (MQ=18)
                                                                                              cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAATGGCGCAGCCAGAGGGGGGCAGCGccc        >  1:1052733/1‑101 (MQ=255)
                                                                                                  aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccct    >  1:485248/1‑99 (MQ=255)
                                                                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccctt   <  1:4041099/101‑4 (MQ=255)
                                                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccccccttc  <  2:1645830/101‑5 (MQ=255)
                                                                                                     |                                                                                                       
ACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCTTCG  >  NC_014034/2249201‑2249400

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG