Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,076 +C 6.7% intergenic (+124/‑118) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0760.→C 6.7% 19.2 0.0 ‑0.8147intergenic (+124/‑118)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/2):  ref base (79/58):  total (85/62)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.43e-01

Only 100 of 177 total aligned reads displayed.

AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.C.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493977‑2494170
                                                                                                                                                                                                             
 aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCag                                                                                                    <  2:2294015/101‑2 (MQ=18)
  gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagc                                                                                                   <  2:2245006/101‑3 (MQ=18)
    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcgc                                                                                                 >  1:4922635/1‑97 (MQ=17)
     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c                                                                                             >  1:2912258/1‑101 (MQ=18)
     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c                                                                                             >  1:1658484/1‑101 (MQ=17)
     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c                                                                                             >  1:1954739/1‑101 (MQ=17)
       cTCCCCCCGGGATATTTGCAGCAAGTTGAAACCGGATGCCCTTCATCTTGCCCGAAATATCCC.GGGGGGTGAG.TCGC..GAAG.CCACAGGGGGGC.AGCG.c.c.cc                                                                                          >  2:1792817/1‑101 (MQ=17)
        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c.ccc                                                                                         >  2:3924517/1‑101 (MQ=17)
         ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c.cccc                                                                                        <  2:4222518/101‑2 (MQ=17)
          cccccGGAATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCG.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c.cccct                                                                                       <  2:4240711/101‑3 (MQ=17)
              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCCTCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGccc                                                                                   >  1:1996975/1‑101 (MQ=17)
                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCcg                                                                                 <  1:4273383/101‑1 (MQ=17)
                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC..AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.C.CCC.TGCCCcgc                                                                                >  1:1106325/1‑100 (MQ=2)
                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.AA.GCCAGAGGGGGCCAGCGCC.C.CCC.TGCCCcgg                                                                                >  1:1500262/1‑101 (MQ=18)
                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGCGAA.TGGC..GAAG.CCAGAGGGGGGCAGCGG.C.C.CCC.TGCCCcgg                                                                                >  2:3780334/1‑101 (MQ=17)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC..AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.C.CCC.TGCCCcgcc                                                                               <  2:2567939/101‑3 (MQ=2)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC..AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.C.CCC.TGCCCcgcc                                                                               >  1:831521/1‑99 (MQ=2)
                  atatTTGCAGGAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.GGGC..GAAG.CCAGAGGGGGGCCGGGC.C.C.CCC.TGCCCcgcc                                                                               >  1:1654281/1‑99 (MQ=17)
                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGCGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCcggc                                                                               >  1:1947906/1‑101 (MQ=17)
                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCcggcg                                                                              >  1:2956470/1‑101 (MQ=17)
                     tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGt                                                                            <  2:1530092/101‑1 (MQ=17)
                      ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTc                                                                           >  1:3754824/1‑101 (MQ=18)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTcc                                                                          >  1:3887436/1‑101 (MQ=17)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTcc                                                                          >  2:1798383/1‑101 (MQ=17)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGGcc                                                                          >  1:3779656/1‑101 (MQ=17)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGCcc                                                                          >  1:3021628/1‑101 (MQ=17)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGG.GAGCG.C.CGCCC.TGCCCCGGCGGTcc                                                                          >  2:1902392/1‑101 (MQ=18)
                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCcggcggggcgt                                                                        >  2:4463382/1‑96 (MQ=17)
                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCCGAGGGGGGC.AGCGCC.C.CCC.TCCCCCAGCCGTCCCTggc                                                                     >  2:887342/1‑101 (MQ=9)
                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCCGCGGTCCGTggcgg                                                                   >  2:3144664/1‑101 (MQ=17)
                               gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTggcggc                                                                  >  1:991197/1‑101 (MQ=17)
                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCt                                                             <  2:5000912/101‑1 (MQ=17)
                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCt                                                             >  1:3099186/1‑101 (MQ=18)
                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCtt                                                            <  2:2912032/101‑1 (MQ=17)
                                     aCCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTg                                                           <  1:4969488/101‑1 (MQ=17)
                                      ccGGATGCCTTTCATCTTCCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGa                                                          <  2:1609947/101‑1 (MQ=17)
                                        ggATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGAcg                                                        <  2:3017367/101‑1 (MQ=17)
                                         gATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGAcgg                                                       >  1:4469323/1‑101 (MQ=18)
                                         gATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGAGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCA.CAC.CCC.TGCCCCGGCGGTCCGTGGCGGGG.CCCTGAcgg                                                       >  1:836473/1‑101 (MQ=12)
                                           tGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGAcgggc                                                     <  1:740217/101‑1 (MQ=17)
                                                ttCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCggggg                                                >  2:76190/1‑101 (MQ=18)
                                                ttCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCggggg                                                >  2:2277111/1‑101 (MQ=18)
                                                ttCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCggggg                                                >  2:3039833/1‑101 (MQ=18)
                                                 tCATCTTGCCCGAAATATCCC.GGGGGGCGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TCCCCCGGCGGTCCGTGGCGGCG.CCTTGACCGGGgggggg                                               >  2:1239428/1‑100 (MQ=9)
                                                  cATCTTGCCCGAAATATCCC.GGGGGGAGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGgcg                                              >  1:531678/1‑101 (MQ=18)
                                                   aTCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGgcgg                                             >  1:2168370/1‑100 (MQ=18)
                                                   aTCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGgcgc                                             >  2:3072130/1‑101 (MQ=18)
                                                    tCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGcgcg                                            >  2:364132/1‑101 (MQ=18)
                                                     cTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCgg                                           <  2:4405102/101‑1 (MQ=18)
                                                     cTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCgg                                           >  1:3428738/1‑101 (MQ=18)
                                                     cTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCgg                                           >  1:3041789/1‑101 (MQ=18)
                                                       tGCCCGAAATATCCCGGGGGGGGGAA.TGGG..G.AA.CCAAGGGGGGGGAAGGG.C.C.CCCTTGCCCCGCCGGCCCG.GGCGGGGCCCTTGACGGGGGGGGGGGGggg                                          >  1:2060910/1‑101 (MQ=255)
                                                       tGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGc                                         <  1:3325569/101‑1 (MQ=18)
                                                       tGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGc                                         <  2:1954602/101‑1 (MQ=18)
                                                        gCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.AA.GCCAGAGGGGGCCAGCGCC.C.CCC.TCCCCCGGCGGTCCGTGGCGGCG.CCTTCACCTGGGGGGGCGCggggc                                        >  2:621723/1‑99 (MQ=9)
                                                         gccGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCAt                                       >  2:2132524/2‑101 (MQ=18)
                                                         cccGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCAt                                       <  1:2606143/101‑1 (MQ=18)
                                                           cGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTa                                     <  2:2942581/101‑1 (MQ=18)
                                                            gAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTaa                                    <  1:2443983/101‑1 (MQ=18)
                                                             aaaTATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGGCGGGCGGGGGCGCGGGCATTAAc                                   <  2:1701948/101‑1 (MQ=18)
                                                             aaaTATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAAc                                   >  2:4202196/1‑101 (MQ=18)
                                                             aaaTATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAAc                                   >  2:2218909/1‑101 (MQ=18)
                                                             aaaTATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAAc                                   >  1:3127500/1‑101 (MQ=18)
                                                             aaaTATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCCCGGGGATTAAc                                   >  2:1249508/1‑101 (MQ=18)
                                                              aaTATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGG.AAGAGCC.C.CCC.TGCCCCGGCGGACAGCGGCGGAG.ACTTGACGGGGGGGGGCCCGGGCGTCAAcc                                  >  1:4022190/1‑101 (MQ=2)
                                                               atatCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGTGGGCATTAACCg                                 <  1:2733563/101‑1 (MQ=18)
                                                               atatCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCg                                 >  2:4307076/1‑101 (MQ=21)
                                                                tatCCG.GGGGGGTGAA.TGCC..GAAG.CCAGAGGGGGGCAGCCC.C.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                <  1:2004203/101‑1 (MQ=25)
                                                                tatCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                >  1:4144164/1‑101 (MQ=21)
                                                                 atCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGaa                               <  2:551369/101‑1 (MQ=21)
                                                                  tCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa                              <  1:4718866/101‑1 (MQ=21)
                                                                  tCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa                              >  1:3623977/1‑101 (MQ=21)
                                                                   ccc.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt                             <  1:234970/101‑1 (MQ=21)
                                                                   ccc.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt                             <  2:3722167/101‑1 (MQ=21)
                                                                    cc.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt                            <  1:1143981/101‑1 (MQ=21)
                                                                     c.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.CAC.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGGGGGGGCGCGGGCGTTAACCGAAATTg                           >  2:298908/1‑101 (MQ=21)
                                                                       ggggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc                          <  1:4782575/101‑1 (MQ=25)
                                                                        gggggTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                         >  2:3177538/1‑101 (MQ=25)
                                                                          gggTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc                       >  2:2360260/1‑101 (MQ=25)
                                                                             tGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa                    <  1:4054721/101‑1 (MQ=25)
                                                                                         gaagccagaGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            >  1:648424/9‑101 (MQ=38)
                                                                                         gaagccagaGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGAGGCTGCGa            >  2:2632481/9‑101 (MQ=37)
                                                                                          aagccagaGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACCGATGCTGCGAt           >  2:4875272/8‑101 (MQ=38)
                                                                                          aagccagaGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAt           >  2:3439191/8‑101 (MQ=38)
                                                                                           agccagaGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATc          <  1:242318/95‑1 (MQ=38)
                                                                                           agccagaGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATc          <  2:4872508/95‑1 (MQ=38)
                                                                                            gccagaGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATcc         >  2:3891015/6‑101 (MQ=38)
                                                                                             ccagaGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        >  2:2167036/5‑101 (MQ=38)
                                                                                              cagaGGGGGGCATCGC.C.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc       <  2:2803191/98‑1 (MQ=255)
                                                                                               agaGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTcc      >  2:546995/3‑100 (MQ=38)
                                                                                               agaGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  2:1177990/3‑101 (MQ=39)
                                                                                               agaGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  1:1358706/3‑101 (MQ=39)
                                                                                                gaGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGc     >  2:3853848/2‑101 (MQ=39)
                                                                                                gaGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGc     >  2:2856510/2‑101 (MQ=39)
                                                                                                 aGGGGGGC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCAcc    >  2:4971476/1‑101 (MQ=255)
                                                                                                  ggggggC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGccc   <  2:3372314/101‑1 (MQ=255)
                                                                                                  ggggggC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTCGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGccc   <  1:3498171/101‑1 (MQ=255)
                                                                                                   gggggC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGGGGGCATTAACCGAAATTGCAACAGAAGCTGCGAACCCCCCCCa  >  2:3510705/1‑101 (MQ=255)
                                                                                                   gggggC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCa  <  2:2147296/101‑1 (MQ=255)
                                                                                                   gggggC.AGCGCC.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCa  <  1:4481659/101‑1 (MQ=255)
                                                                                                                                                                                                             
AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.C.C.CCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493977‑2494170

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG