Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,160 +C 100% intergenic (‑136/+140) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1601.→C100.0% 530.4 170intergenic (‑136/+140)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (30/140):  ref base (0/0):  total (30/140)

Only 100 of 199 total aligned reads displayed.

CCGGTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGG.CTTC.GGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACG  >  NC_014034/2640060‑2640256
                                                                                                        |                                                                                                 
ccGGTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTgg                                                                                                    <  1:1946152/101‑1 (MQ=255)
ccGGTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTgg                                                                                                    <  1:620985/101‑1 (MQ=255)
   gTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGG.cct                                                                                                <  2:3713894/101‑3 (MQ=255)
    ttGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGG.cctt                                                                                               <  2:747182/101‑4 (MQ=255)
    ttGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGG.cctt                                                                                               <  2:3152263/101‑4 (MQ=255)
     tGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGG.ccttc                                                                                              >  2:2952074/1‑97 (MQ=255)
      gCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCCGCCCCCCTC.TTGG.ccttcg                                                                                             <  2:8387/101‑6 (MQ=255)
          gTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCa                                                                                         <  1:2494060/101‑1 (MQ=255)
           ttATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAAGGGGGGGGGCGGCCCCCCCC.TTGG.CGTC.gcgccc                                                                                        >  2:2020790/1‑96 (MQ=255)
           ttATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCaa                                                                                        <  1:3175965/101‑1 (MQ=255)
           ttATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTAAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGCGGCGCTGCCACCCTC.ATGGCCTTC.GGACaa                                                                                        >  2:1535972/1‑101 (MQ=255)
             atgatgCGCCGGGTATGTGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAAtt                                                                                      <  2:4539297/101‑1 (MQ=255)
             atgatgCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAAtt                                                                                      <  1:2952302/101‑1 (MQ=255)
                atgCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTcac                                                                                   <  1:1761420/101‑3 (MQ=255)
                 tgCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCAcc                                                                                  <  2:4311934/101‑1 (MQ=255)
                    gcCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCAccccc                                                                               <  1:880941/101‑1 (MQ=255)
                    gcCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCAccccc                                                                               <  1:4437446/101‑1 (MQ=255)
                    gcCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCAccccc                                                                               <  1:1049681/101‑1 (MQ=255)
                      cGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCg                                                                             <  2:2195002/101‑1 (MQ=255)
                      cGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCg                                                                             <  2:3666982/101‑1 (MQ=255)
                      cGGGTATGCGACGCGGCGGCAGGCCTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCGCCCCCCg                                                                             <  1:751880/101‑1 (MQ=255)
                          tATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGt                                                                         <  1:3111059/101‑1 (MQ=255)
                           aTGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTa                                                                        <  2:1149663/101‑1 (MQ=255)
                             gCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTAtt                                                                      <  2:919490/101‑1 (MQ=255)
                              cggcggggcgtcAGGCGTGTCACCCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTAttt                                                                     <  1:2194041/95‑1 (MQ=21)
                              cGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTAttt                                                                     <  1:2462549/101‑1 (MQ=255)
                              cGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTAttt                                                                     <  1:3995140/101‑1 (MQ=255)
                               gACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTg                                                                    <  2:596595/101‑1 (MQ=255)
                                aCGCGGCGGGAGGCGTGTCAACCGTGGCGG.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTgg                                                                   <  2:1238442/101‑1 (MQ=25)
                                aCGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCTGAGGTATTTgg                                                                   <  1:4106284/101‑1 (MQ=37)
                                  gcggcgGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGag                                                                 <  2:124730/101‑2 (MQ=255)
                                    ggcggcAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGCGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCa                                                               <  1:4246106/101‑1 (MQ=37)
                                        gcAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGAt                                                           <  1:4822526/101‑1 (MQ=37)
                                         cAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                          <  1:3322436/101‑1 (MQ=37)
                                           ggCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                        <  2:2929298/101‑1 (MQ=25)
                                           gccGTGCCAACCGT.GCGCTTTTTGCG.AGGCAAAAGGGGGGGCCCTCCCCCCCCC.TGGC.CTTCGGGCAAATTCCCCCCCCGAGGTATTTGGAGCAAGATGaa                                                        <  2:1571107/99‑1 (MQ=255)
                                             cGTGTCACCCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                      <  2:1998669/101‑2 (MQ=25)
                                             cGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                      <  1:434024/101‑2 (MQ=25)
                                                gtCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGTGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                   >  1:4644059/1‑97 (MQ=21)
                                                 tccaCCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCa                                                 <  2:4055416/98‑1 (MQ=21)
                                                 tCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCGCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCa                                                 >  2:1060519/1‑101 (MQ=21)
                                                 tCAACCGTGGCGC.TTTGGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCa                                                 <  2:810723/101‑1 (MQ=21)
                                                  cAACCGTGGCGC.TTTTGCGGAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCAt                                                <  1:2839818/101‑1 (MQ=21)
                                                   aaCCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTTACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                               <  2:3879721/101‑1 (MQ=25)
                                                   aaCCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                               <  1:2753977/101‑1 (MQ=25)
                                                   aaCCGTGGCGC.TTTTGCGCAGGCAAAA.AGGGGGGGCTGCCCCCCCCTTTGC.CCTT.CGCCCATTCACCCCCCCAGGGATTTTGGGCAAAATGaaagacccag                                                >  1:5053521/1‑94 (MQ=255)
                                                    aCCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                              <  1:3436251/101‑1 (MQ=25)
                                                     ccGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                             <  2:173779/101‑1 (MQ=25)
                                                        tGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                          <  1:2159917/101‑1 (MQ=25)
                                                        tGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                          <  1:1426972/101‑1 (MQ=25)
                                                        tGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                          <  1:3476322/101‑1 (MQ=25)
                                                        tGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                          <  1:1368349/101‑1 (MQ=25)
                                                        gggCGC.TTTTGCGGAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                          <  1:1756064/100‑1 (MQ=21)
                                                         ggCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                         <  2:2736335/101‑1 (MQ=25)
                                                          gcgc.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                        <  2:1596138/101‑1 (MQ=25)
                                                          gcgc.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                        <  2:4586745/101‑1 (MQ=25)
                                                            gc.TTTGGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCt                                      <  2:1492289/101‑1 (MQ=21)
                                                               ttttGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                    <  2:4296686/101‑1 (MQ=25)
                                                                   gcgcAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                <  2:4826725/101‑1 (MQ=25)
                                                                   gcgcAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                <  2:2550142/101‑1 (MQ=25)
                                                                   gcgcAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                <  2:691865/101‑1 (MQ=25)
                                                                    ggcAGGCAAAA.CGGTGGCGCTGCCGCCGCC.TTGGCCTTC.CGGGAATTGACCCCCCGAGGTGTTTGGGGGAAGATG.AAAGGCCATGGGTTCATCTTGCCTCa                               <  2:3737374/100‑1 (MQ=255)
                                                                    cgcAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                               <  1:1089099/101‑1 (MQ=25)
                                                                     gcAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaa                              <  1:4774475/101‑1 (MQ=25)
                                                                      gccccgaaa.AGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                            <  2:2131694/95‑1 (MQ=14)
                                                                      gccccgaaa.AGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                            <  1:3887285/95‑1 (MQ=14)
                                                                      cAGGCAAAA.GGGGGGCGCCGCCCCCCTC.TTGGCCTTC.GGCCTATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGGTGATCTTGGCTCaac                             >  2:3949109/1‑100 (MQ=18)
                                                                       ccccgaaa.AGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAata                           <  1:4970263/96‑1 (MQ=17)
                                                                       aGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                            <  1:4665782/101‑1 (MQ=21)
                                                                        ggCAAAA.GGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCACCTTGCCTCAAata                           <  1:3238144/101‑1 (MQ=21)
                                                                          cgaaa.AGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                        <  2:439416/99‑1 (MQ=17)
                                                                           gaaa.AGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATccc                       >  1:3780954/2‑101 (MQ=17)
                                                                            aaa.AGGGGGCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                      <  2:2360025/101‑1 (MQ=17)
                                                                              aaagggggCGCTGCCCCCCTC.TTGGCCTTT.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                     >  1:4509162/4‑101 (MQ=18)
                                                                                agggggCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                   <  2:3252288/100‑1 (MQ=18)
                                                                                 gggggCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                  <  2:1908361/101‑1 (MQ=18)
                                                                                 gggggCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                   <  2:3521188/101‑1 (MQ=21)
                                                                                  ggggCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCCCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                 <  1:106036/101‑2 (MQ=18)
                                                                                  ggggCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                 >  2:2845192/1‑101 (MQ=18)
                                                                                   gggCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggtggg                 <  2:3580942/101‑1 (MQ=21)
                                                                                    ggCGCTGCCCCCCTC.TTGGCCTTC.GGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                <  1:3928763/101‑1 (MQ=25)
                                                                                       gcTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc            >  2:1413667/1‑95 (MQ=21)
                                                                                       gcTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGta             >  1:1463254/1‑100 (MQ=25)
                                                                                       gcTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc             <  2:1463124/101‑1 (MQ=25)
                                                                                       gcTGCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCCGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggggaggg            >  2:3599364/1‑96 (MQ=18)
                                                                                          gCCCCCCTC.TTGGCCTTC.GGCCACTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg          <  1:4928576/101‑1 (MQ=25)
                                                                                          gCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg          <  2:4038199/101‑1 (MQ=37)
                                                                                          gCCCCCCTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg          <  1:1181267/101‑1 (MQ=37)
                                                                                            cccccTC.TTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggggaggcgaagc       >  1:2604683/1‑91 (MQ=25)
                                                                                            cccccTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa        >  1:1640045/1‑101 (MQ=37)
                                                                                            cccccTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa        <  2:4248417/101‑1 (MQ=37)
                                                                                             ccccTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa       <  2:4965158/101‑1 (MQ=37)
                                                                                             ccccTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa       <  1:2575477/101‑1 (MQ=37)
                                                                                              cccTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg      <  1:2337068/101‑1 (MQ=37)
                                                                                               ccTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg     >  2:4735993/1‑101 (MQ=37)
                                                                                               ccTC.TTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg     <  2:4643736/101‑1 (MQ=37)
                                                                                                ctc.tTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa    >  1:2784059/1‑101 (MQ=37)
                                                                                                 tc.tTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc   >  1:2268825/1‑101 (MQ=37)
                                                                                                  c.tTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg  >  1:4614544/1‑101 (MQ=37)
                                                                                                  c.tTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg  >  1:2970171/1‑101 (MQ=37)
                                                                                                        |                                                                                                 
CCGGTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTC.TTGG.CTTC.GGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACG  >  NC_014034/2640060‑2640256

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG