Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,849	+G	100%	intergenic (‑181/+112)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,849	1	.→G	100.0%	58.6	19	intergenic (‑181/+112)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (2/17):  ref base (0/0):  total (2/17)

GTTCGGGCGGAATAGGAAA.CGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGG  >  NC_014034/2747749‑2747943                                                                                                       |                                                                                                gtgcGGGCGGAATGGGAAA.CGCGCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc                                                                                                  <  2:2289214/98‑1 (MQ=255) gTTCGGGCGGAATAGGAAC.CGCGCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc                                                                                                  <  2:3998044/101‑1 (MQ=255) gTTCGGGCGGAATAGGAAC.CGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc                                                                                                  <  2:1272986/101‑1 (MQ=255)  ttCGGGCGGAATAGGAAC.CGCGCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.g                                                                                                <  2:3742082/101‑1 (MQ=255)   tCGGGCGGAATAGGAAA.CGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCTGCgcccggccc.gg                                                                                               >  1:3391887/1‑100 (MQ=255)    ggggCGGAAGAGAACC.CGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggc                                                                                              <  2:1843962/100‑3 (MQ=38)    ggggCGAAATAGGAAA.CGCGCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggc                                                                                              <  1:402857/100‑3 (MQ=255)    cGGGGGGAATGGGAAC.CGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggc                                                                                              <  1:1953914/101‑3 (MQ=255)     ggccgaaaTAGGAAC.CGCGCCCCGCTTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcg                                                                                             <  2:403430/95‑4 (MQ=38)        cGGAATAGAAAA.CGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcgggc                                                                                          <  2:849224/101‑7 (MQ=38)         ggAATAGGAAC.CGCGACCCGCTTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcgggcg                                                                                         <  2:556721/101‑8 (MQ=38)         ggAATAGGAAA.CGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcggggg                                                                                         >  2:225842/1‑94 (MQ=255)         ggAAAAAGAAACCGCGCCCCGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcgggc                                                                                          <  1:4447662/101‑7 (MQ=25)            aTAGGAAC.CCCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgaga                                                                                       <  2:2092251/101‑1 (MQ=25)            aTAGGAAA.CGCGCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgaga                                                                                       <  2:3085245/101‑1 (MQ=37)             tAGGAAC.CGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGaa                                                                                      <  1:4643178/101‑1 (MQ=37)             tAGGAAC.CCCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCCGCGCCCGGCCCGGCGGGCGAGaa                                                                                      <  1:4064584/101‑1 (MQ=25)              aGGAAC.CGCGCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAg                                                                                     <  2:4846053/101‑1 (MQ=37)              aGGAAA.CGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAg                                                                                     >  2:3724733/1‑101 (MQ=255)               gaaaa.CCCGCCCCGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                    <  1:3725011/99‑1 (MQ=25)                  aa.CGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcc                                                                                 <  1:4016287/101‑1 (MQ=25)                   accgcgCCCCGGTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcc                                                                                 <  2:3423423/99‑1 (MQ=21)                   accgcgCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcc                                                                                 <  1:3750972/99‑1 (MQ=25)                   accgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcc                                                                                 <  1:3859787/99‑1 (MQ=25)                    ccgcgACCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgccc                                                                                <  2:561100/100‑1 (MQ=25)                     cgcgCCCCGGTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcccg                                                                               <  2:3978245/101‑1 (MQ=21)                     cgcgACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcccg                                                                               >  1:4888539/1‑101 (MQ=255)                      ccgACCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcccgc                                                                              <  1:4443637/100‑1 (MQ=25)                           ccgttttcccccGCGCGCCGCGCGCTGTTGCGGCATGACCGGTCCAGCCCGCACCACAGAGGGCAGCGCCGGGCCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                        <  2:2278308/93‑3 (MQ=12)                            cggctccccccGCCCGCAGCGCCGGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                       <  2:4979845/94‑4 (MQ=21)                             ccgTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                      <  2:251635/100‑5 (MQ=25)                             ccccccccccGCCCGCAGCCCCCGTTTGCGGCATGCCCGCTTCAGCCCGCCCCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                      <  1:1793972/95‑5 (MQ=18)                                ttcccccGCCCGCAGCGCCCGGTTGCGGCATGCCCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                   <  2:3005922/98‑8 (MQ=21)                                ttcccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                   <  1:4834653/98‑8 (MQ=21)                                                     gTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCCGCCCGAGGGCCTTTGCGc                                               >  2:880316/1‑101 (MQ=18)                                                                                        ggCGGCGCCCGCCCCGGCGGGCGAGATGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGcc            <  1:3227835/101‑1 (MQ=17)                                                                                                  ggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg  >  2:2821873/1‑101 (MQ=18)                                                                                                  ggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg  >  2:1751191/1‑101 (MQ=18)                                                                                                       |                                                                                                GTTCGGGCGGAATAGGAAA.CGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGG  >  NC_014034/2747749‑2747943 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG