Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_014034 | 3,214,790 | +A | 5.9% | intergenic (‑46/+12) | dctQ3 ← / ← dctP3 | TRAP C4‑dicarboxylate transport system permease subunit DctQ/TRAP C4‑dicarboxylate transport system permease subunit DctP |
Read alignment evidence... | ||||||||||
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seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_014034 | 3,214,790 | 10 | .→A | 5.9% | 2.3 0.0 ‑0.0 | 34 | intergenic (‑46/+12) | dctQ3/dctP3 | TRAP C4‑dicarboxylate transport system permease subunit DctQ/TRAP C4‑dicarboxylate transport system permease subunit DctP |
Reads supporting (aligned to +/- strand): new base (2/0): ref base (31/1): total (33/1) | ||||||||||
Fisher's exact test strand distribution p-value = 1.00e+00 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01 |
TCGCGATGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCAGAACCTC > NC_014034/3214690‑3214888
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tcgcGATGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGagcc < 1:3891883/101‑1 (MQ=255)
cgcgATGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGcgcc..........g > 2:981178/1‑101 (MQ=255)
cgcgATGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGcgcc..........g > 2:3446218/1‑101 (MQ=255)
cgcgATGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGcgcc..........g > 2:2527735/1‑101 (MQ=255)
cgcgATGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGcgcc..........g > 1:968369/1‑101 (MQ=255)
tgATGAGCGCGGCAATCAGCCCCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGcgcc..........gtc < 1:2910237/100‑3 (MQ=255)
gATGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGggcc..........gtca > 2:2910024/1‑98 (MQ=255)
gATGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtcc < 1:3755856/101‑1 (MQ=255)
gATGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtcc < 2:4173278/101‑1 (MQ=255)
gATGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtcc < 2:2874870/101‑1 (MQ=255)
gATGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtcc < 2:1862244/101‑1 (MQ=255)
gATGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtcc < 2:1920485/101‑1 (MQ=255)
aagAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccg > 2:1496258/3‑101 (MQ=255)
aTGAGGGCGCCAATCAGCCCCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccg < 2:2063117/101‑1 (MQ=255)
aTGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccg > 1:2351580/1‑101 (MQ=255)
aTGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccg < 1:4269174/101‑1 (MQ=255)
tGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccgg < 2:1230578/101‑2 (MQ=255)
tGAGCGCGGCAATCAGCACCTCGTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccgg < 1:4576686/101‑2 (MQ=255)
gAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggc < 1:3994664/101‑3 (MQ=255)
gAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggc > 1:1902890/1‑99 (MQ=255)
gAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggc < 2:734447/101‑3 (MQ=255)
gAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggc > 2:4245261/1‑99 (MQ=255)
aGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........GGGCGcc..........gtccggcc > 1:2706266/1‑98 (MQ=255)
cgcgGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggcgcc < 2:3110074/101‑6 (MQ=255)
cgcgGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggcgcc < 1:4693784/101‑6 (MQ=255)
cgcgGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggcgcc < 1:2075188/101‑6 (MQ=255)
cgcgGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggcgcc < 2:2628321/101‑6 (MQ=255)
cgcgGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........ggccggcgcc > 1:4817398/1‑96 (MQ=255)
gcgGCAATCAGCCCCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggcgccg < 2:4213547/101‑7 (MQ=255)
gcgGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGGGcc..........gtccggcgccc > 2:3173918/1‑95 (MQ=255)
cgGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggcgccgc > 2:1185227/1‑94 (MQ=255)
cgGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGACGTGGAAGGAAAGGGAAAGGG..........GGGAGac..........gtccggcgccgc > 2:2692723/1‑94 (MQ=255)
gCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggcgccgccc < 1:3503875/101‑10 (MQ=255)
gCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggcgccgccc > 1:2224238/1‑92 (MQ=255)
gCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggcgccgccc < 1:573178/101‑10 (MQ=255)
gCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggcgccgccc < 1:2904766/101‑10 (MQ=255)
cAATCAGCACCTCTTCGGCCCGGTCGAGGATGCTCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggcgccgcccg < 1:4324324/101‑11 (MQ=255)
cAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGcc..........gtccggcgccgcccg > 2:1237620/1‑91 (MQ=255)
aTGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGGCGGCGCCGTCCGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGa > 1:538671/1‑101 (MQ=255)
cagcaTGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGGCGGCGCCGTCCGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGGcggcg > 2:1066504/1‑101 (MQ=255)
agcaTGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGGCGGCGCCGTCCGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGAcggcgg > 1:4034047/1‑101 (MQ=255)
agcaTGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGCCGTCCGGCGCAGCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGAccgcgg > 1:1164736/1‑101 (MQ=255)
gcaTGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGGCGGCGCCGTCCGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACggcggc > 1:3579014/1‑101 (MQ=255)
caTGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGGCGGCGCCGTCCGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGAcggnnngn > 1:3583035/1‑96 (MQ=255)
caTGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGGCGGCGCCGTCCGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGgcggcg > 2:4249343/1‑101 (MQ=255)
caTGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGGCGGCGCCGTCCGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGgcggcg > 1:2935588/1‑101 (MQ=255)
tGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGGCGGCGCCGTCCGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGAt > 2:1330855/1‑101 (MQ=255)
ggcCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGGCGGCGCCGTCCGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGGTGGtt > 2:982609/1‑101 (MQ=255)
ggcCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGGCGGCGCCGTCCGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGtt > 2:3326722/1‑101 (MQ=255)
ggcCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGGCGGCGCCGTCCGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGtt > 2:1107327/1‑101 (MQ=255)
ggcCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGCCGTCCGGCGCAGCCCGCCGGTCTTATTCCGCCGTCGCGGACTTGACGGCGGCGGTgggt > 1:2754679/1‑99 (MQ=255)
gcCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGGCGGCGCCGTCCGGCGCC..........GCCCGCAGGTCTTATTCCGCCGCCGCGGCCTTGACGGCGGCGATGGgtt > 1:1060573/1‑101 (MQ=255)
cccccGCTTAGGTCGTGGAAGGAAAGGGAAAGGGCGGCGCCGTCCGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTc > 2:4635117/1‑101 (MQ=255)
ccccGCTTAGGTCGTGGAAGGAAAGGGAAAGGGCGGCGCCGTCCGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGGTTCg > 2:4272794/1‑101 (MQ=255)
cggcgccgtccggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGTAcc > 1:4001930/11‑101 (MQ=255)
cggcgccgtccggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCAcc > 2:158113/11‑101 (MQ=255)
ggcgccgtccggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCg > 2:2496459/10‑101 (MQ=255)
ggcgccgtccggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCg > 2:1417779/10‑101 (MQ=255)
ggcgccgtccggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCg > 1:810700/10‑101 (MQ=255)
gcccccgcccggccCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCg < 2:4033744/92‑1 (MQ=255)
gcgccgtccggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCgg < 1:1417906/93‑1 (MQ=255)
ccccgccgggcgCC..........CCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCggg < 1:864727/93‑1 (MQ=255)
cgtccggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTc > 2:4818285/5‑101 (MQ=255)
cgtccgccgcc..........ccCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTc < 2:457735/94‑1 (MQ=255)
gtccggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGGGGTGGTTTCGGCGCCGATCCCCCCCGGCATTTTGTCCTTCACCCGGGGCa > 2:4300181/4‑101 (MQ=255)
gtccggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCa > 2:3403782/4‑101 (MQ=255)
tgctgcgcC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCAg > 2:2656707/5‑101 (MQ=255)
tccggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCAg > 2:2071995/3‑101 (MQ=255)
tccggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCAg > 1:4861566/3‑101 (MQ=255)
tccggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCAg > 2:2237100/3‑101 (MQ=255)
cggccgcc..........ccCCGGAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTccat < 2:2598028/97‑4 (MQ=255)
ccggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCAGa > 2:3138709/2‑101 (MQ=255)
cggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCAGaa > 1:309946/1‑101 (MQ=255)
cggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCAGaa > 2:1229403/1‑101 (MQ=255)
cggcgCC..........CCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCAGaa < 2:2357909/101‑1 (MQ=255)
ggcgCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCAGAAc > 1:4996265/1‑101 (MQ=255)
gccgcc..........ccCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCAGAAc < 2:3588565/99‑1 (MQ=255)
gcgcC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGCCAGAAcc > 2:2280543/1‑101 (MQ=255)
cgcc..........gcCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCAGAACct < 1:1229520/101‑1 (MQ=255)
gcc..........gccCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCTTCATCCCCCGGGGTCAGAACctc > 2:1464178/1‑101 (MQ=255)
gcc..........gccCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCAGAACctc < 1:4818612/101‑1 (MQ=255)
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TCGCGATGAGCGCGGCAATCAGCACCTCTTCGGCCCGGTCGAGGATGCGCAGCATGGCGGCCCCCGCTTAGGTCGTGGAAGGAAAGGGAAAGGG..........CGGCGCC..........GCCCGCAGGTCTTATTCCGCCGTCGCGGCCTTGACGGCGGCGATGGTTTCGGCGCCGATCCGCTCGGCCATTTCGTCATGCACCGGGGTCAGAACCTC > NC_014034/3214690‑3214888
Base quality scores: 
ATCG
< 3 ≤
ATCG
< 18 ≤
ATCG
< 25 ≤
ATCG
< 33 ≤
ATCG
< 40 ≤
ATCG
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