BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,256,195	+G	100%	coding (1338/1734 nt)	recJ →	single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,256,195	1	.→G	100.0%	486.5	128	coding (1338/1734 nt)	recJ	single‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand): new base (63/65): ref base (0/0): total (63/65)

Only 100 of 131 total aligned reads displayed.


GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGA.A.CCGCGATG.G.AA.GGCT.GCCGA.G.TGTTCGCCCGGCAGGG..C.GC.G.GCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256108‑3256278


tctcGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.t                                                                                     <  1:2472541/98‑1 (MQ=255)


ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.t                                                                                     >  1:1751217/1‑101 (MQ=255)


  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.G.CACGGCTGGCCGA.g.ttg                                                                                   <  2:4006499/101‑3 (MQ=255)


    gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.ttgct                                                                                 >  2:1873144/1‑97 (MQ=255)


    gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.ttgct                                                                                 >  1:279822/1‑97 (MQ=255)


    gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.ttgct                                                                                 >  1:2555655/1‑97 (MQ=255)


     aGGGGCTTTTGCTTAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.G.CACGGCTGGCCGA.g.ttgctc                                                                                <  2:1413648/101‑6 (MQ=255)


     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.ttgctc                                                                                >  2:4666067/1‑96 (MQ=255)


        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.ttgctcgcc                                                                             <  2:1901834/101‑9 (MQ=255)


        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.ttgctcgcc                                                                             >  1:4851370/1‑93 (MQ=255)


         gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGccc                                                                             >  2:4043266/1‑101 (MQ=255)


         gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.G.CACGGCTGGCCGA.GTTGCTCGccc                                                                             <  2:2214537/101‑1 (MQ=255)


           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCgg                                                                           <  1:2812114/101‑1 (MQ=255)


            tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGc                                                                          <  1:1201644/101‑1 (MQ=255)


            tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGc                                                                          >  1:4629387/1‑101 (MQ=255)


             ttGCTCAAGGGCTGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCCGCa                                                                         >  1:4128457/1‑101 (MQ=255)


             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGcc                                                                         >  2:1292603/1‑100 (MQ=255)


              tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAg                                                                        >  1:1374178/1‑101 (MQ=255)


              tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAg                                                                        <  2:2950074/101‑1 (MQ=255)


               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCCgg                                                                       >  2:1347881/1‑101 (MQ=255)


               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.G.CACGGCTGGCCGA.GTTGCTCGCCCGGCAgg                                                                       <  1:51266/101‑1 (MQ=255)


                cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAggg                                                                      <  1:3983003/101‑1 (MQ=255)


                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGg..c                                                                   <  1:897208/101‑1 (MQ=255)


                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGg..c                                                                   <  1:1870849/101‑1 (MQ=255)


                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGg..c.g                                                                 >  1:3508661/1‑101 (MQ=255)


                    aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGg..c.ggg                                                               >  1:2526948/1‑99 (MQ=255)


                      ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCCGGG..C.GC.g.gg                                                           >  1:1062966/1‑100 (MQ=255)


                       gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GC.g.ggc                                                          <  1:4290424/101‑3 (MQ=255)


                       gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GC.g.ggc                                                          <  1:1103889/101‑3 (MQ=255)


                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GC.g.ggcac                                                        <  1:3410614/101‑5 (MQ=255)


                          gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GC.g.gggacg                                                       >  2:1124347/1‑96 (MQ=255)


                          gcggGCACCGCATGGCCGCGGGTCTCACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GG.g.ggcacg                                                       <  2:4859418/101‑6 (MQ=255)


                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGGTTGCCCCGCACGG..C.GCGG.GCA.c.gg                                                     >  1:1559104/1‑101 (MQ=255)


                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.c.gg                                                     <  2:4779617/101‑1 (MQ=255)


                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.c.gg                                                     >  1:3281301/1‑101 (MQ=255)


                             ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.c.gggc                                                   <  2:4816164/101‑3 (MQ=255)


                              gCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GC.g.ggcccgggcg                                                   >  1:737715/1‑92 (MQ=255)


                               cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GC.g.gggccgggggg                                                  >  1:3083632/1‑91 (MQ=255)


                                aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.c.gggcggc                                                <  1:1011539/101‑6 (MQ=255)


                                  cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGcggccc                                               <  1:4918924/101‑1 (MQ=255)


                                  cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCCA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGcggccc                                               <  2:4492720/101‑1 (MQ=255)


                                    cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CGGG.G.GCA.CGGGgggcccgg                                             >  1:1322353/1‑101 (MQ=255)


                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGcggcccggcc                                           <  2:43663/101‑1 (MQ=255)


                                       ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GGA.CGGGCGGGCCGGCCg                                          >  2:2069807/1‑101 (MQ=255)


                                       ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCg                                          >  1:2145889/1‑101 (MQ=255)


                                         ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGAt                                        <  2:3508391/101‑1 (MQ=255)


                                         ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGAt                                        <  2:1810577/101‑1 (MQ=255)


                                           gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATGAC.AC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATcc                                      >  2:699078/1‑100 (MQ=255)


                                           gcgGGTCTGACCCTGGCCGAGGCGCAGATGGAGC.CCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCt                                      <  1:4954745/101‑1 (MQ=255)


                                            cgGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTg                                     <  1:1755191/101‑1 (MQ=255)


                                            cgGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTg                                     >  1:1526449/1‑101 (MQ=255)


                                             gggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCCGCCGATCTgg                                    >  2:1363505/1‑100 (MQ=255)


                                              ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGGGGGCCCGCCGGTCTGCg                                   >  2:1702160/1‑101 (MQ=255)


                                              ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCg                                   <  1:2635195/101‑1 (MQ=255)


                                              ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCg                                   <  2:4682627/101‑1 (MQ=255)


                                              ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCg                                   >  2:4142690/1‑101 (MQ=255)


                                              ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCg                                   >  2:1327489/1‑101 (MQ=255)


                                               gTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGGCCGGCCGATCTGCgg                                  >  1:1524351/1‑101 (MQ=255)


                                               gTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCgg                                  <  1:3975962/101‑1 (MQ=255)


                                                tCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGAGG.TGCTCGCCCGGCAGGG..C.GC.GCGCACC.GGGGGGCCGGCCGCTTTGCCGc                                 >  2:3509414/1‑101 (MQ=255)


                                                tCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGc                                 <  1:1982896/101‑1 (MQ=255)


                                                 cTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCt                                >  1:1276473/1‑101 (MQ=255)


                                                  tGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCTGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCtt                               <  2:1593756/101‑2 (MQ=255)


                                                  tGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGGGGCCCCGCCGGTCTGCGGGTg                               >  1:802149/1‑101 (MQ=255)


                                                   gACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTgg                              <  1:2616883/101‑1 (MQ=255)


                                                   gACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTgg                              >  1:4518389/1‑101 (MQ=255)


                                                      cctggCCGAGGCGCAGCTGGA.ACCCGCGATG.GCGC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACg                           <  1:4331629/97‑1 (MQ=255)


                                                        tggCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACggg                         <  1:624457/99‑1 (MQ=255)


                                                             gaggcgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCt                    <  2:2201612/91‑1 (MQ=255)


                                                             gaggcgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                    <  2:1411706/91‑1 (MQ=255)


                                                              gggcgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                   <  1:4546943/92‑1 (MQ=255)


                                                              aggcgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                   <  2:4047142/92‑1 (MQ=255)


                                                               ggcgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTGa                  >  2:3497188/9‑101 (MQ=255)


                                                                gcgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                 <  1:2357771/94‑1 (MQ=255)


                                                                gcgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                 <  1:4143007/94‑1 (MQ=255)


                                                                 cgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGTCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                <  2:158647/95‑1 (MQ=255)


                                                                  gcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGc               >  2:2657273/6‑101 (MQ=255)


                                                                  gcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc               <  1:3096687/96‑1 (MQ=255)


                                                                   cagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGTAGGGCCC.GG.G.GAA.G.GGCGGTCCGGCCGATCTGCGGCTGGACGGGCTTCTGAAGcc              >  2:2838301/5‑101 (MQ=255)


                                                                   cagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc              <  2:2801378/97‑1 (MQ=255)


                                                                   cagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGACGcc              <  2:4279401/97‑1 (MQ=255)


                                                                    gagctggatcccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc              <  2:711062/91‑1 (MQ=255)


                                                                    agctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCg             >  1:4955129/4‑101 (MQ=255)


                                                                    agctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCg             >  1:1813104/4‑101 (MQ=255)


                                                                      ctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGGGGGCTCCTGATGCCggg           >  2:333202/2‑101 (MQ=255)


                                                                      ctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCggg           >  1:3483782/2‑101 (MQ=255)


                                                                       tGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGGTGGGCGGGGTCCCGAAGCCcggg          >  2:3701572/1‑100 (MQ=255)


                                                                       tGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCgggg          >  2:2793437/1‑100 (MQ=255)


                                                                       tGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc          <  1:1207695/101‑1 (MQ=255)


                                                                       tGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc          <  2:251222/101‑1 (MQ=255)


                                                                         ggaacccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGGGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCggggg         >  2:2070732/6‑99 (MQ=255)


                                                                         ggaacccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgcg         >  1:3286955/6‑101 (MQ=255)


                                                                          gaacccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGGCGGgggc        >  1:1089849/5‑101 (MQ=255)


                                                                          gaacccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgcgc        <  2:2145741/97‑1 (MQ=255)


                                                                              ccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg    <  1:1327610/101‑1 (MQ=255)


                                                                              ccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg    <  1:4666390/101‑1 (MQ=255)


                                                                                gcgATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCCGGCGCcgcggc  >  2:2243740/1‑99 (MQ=255)


                                                                                gcgATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc  <  1:2657458/101‑1 (MQ=255)


                                                                                gcgATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc  <  2:1509941/101‑1 (MQ=255)


                                                                                gcgATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc  <  2:741093/101‑1 (MQ=255)


GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGA.A.CCGCGATG.G.AA.GGCT.GCCGA.G.TGTTCGCCCGGCAGGG..C.GC.G.GCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256108‑3256278

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG