Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,201 +T 100% coding (1344/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2011.→T100.0% 480.3 126coding (1344/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (54/72):  ref base (0/0):  total (54/72)

Only 100 of 150 total aligned reads displayed.

GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGA.A.CCGCGATG.G.AA.GGCT.GCCGA.G.TGTTCGCCCGGCAGGG..C.G.C.G.GCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA.CGCCCGCGCTGATCGA  >  NC_014034/3256108‑3256293
                                                                                                        |                                                                                                     
tctcGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.t                                                                                                      <  1:2472541/98‑1 (MQ=255)
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.t                                                                                                      >  1:1751217/1‑101 (MQ=255)
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.t                                                                                                      <  2:1959359/101‑1 (MQ=255)
 gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.tt                                                                                                     >  2:2160450/1‑100 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.G.CACGGCTGGCCGA.g.ttg                                                                                                    <  2:4006499/101‑3 (MQ=255)
    gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.ttgct                                                                                                  >  2:1873144/1‑97 (MQ=255)
    gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.ttgct                                                                                                  >  1:2555655/1‑97 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.ttgctcgcc                                                                                              >  2:4727717/1‑93 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.ttgctcgcc                                                                                              <  2:1901834/101‑9 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.g.ttgctcgcc                                                                                              >  1:4851370/1‑93 (MQ=255)
         gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGccc                                                                                              >  1:2459081/1‑101 (MQ=255)
         gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.G.CACGGCTGGCCGA.GTTGCTCGccc                                                                                              <  2:2214537/101‑1 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCgg                                                                                            <  1:2812114/101‑1 (MQ=255)
            tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGc                                                                                           <  1:1201644/101‑1 (MQ=255)
             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGcc                                                                                          >  2:1292603/1‑100 (MQ=255)
              tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAg                                                                                         >  1:1374178/1‑101 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCCgg                                                                                        >  2:1347881/1‑101 (MQ=255)
                cTCAAGGGCGTGGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.G.CACGGCTGGCCGA.GTTGCTCGCCCGGCAggg                                                                                       <  2:920933/101‑1 (MQ=255)
                cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAggg                                                                                       <  1:3983003/101‑1 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGg..c                                                                                    <  1:1870849/101‑1 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGg..c                                                                                    <  1:897208/101‑1 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGg..c.g                                                                                  >  1:3508661/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGg..g.g.c                                                                                >  2:2618333/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCCGCCGGg..g.g.c                                                                                >  2:1179403/1‑101 (MQ=255)
                    aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGg..c.ggg                                                                                >  1:2526948/1‑99 (MQ=255)
                      ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCCGGG..C.G.C.g.gg                                                                           >  1:1062966/1‑100 (MQ=255)
                       gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGG.GTTGCCCGCCCGGCAgggggggggg                                                                                >  2:1390850/1‑94 (MQ=255)
                       gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.C.g.ggc                                                                          <  1:1103889/101‑3 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.C.g.ggcac                                                                        <  1:3410614/101‑5 (MQ=255)
                          gcggGCACCGCATGGCCGCGGGTCTCACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.G.g.ggcacg                                                                       <  2:4859418/101‑6 (MQ=255)
                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.c.gg                                                                     >  1:3281301/1‑101 (MQ=255)
                             ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..G.G.CGG.GCA.c.gggg                                                                   >  1:3980762/1‑99 (MQ=255)
                             ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.c.gggc                                                                   <  2:4816164/101‑3 (MQ=255)
                              gCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.C.g.ggcccgggcg                                                                   >  1:737715/1‑92 (MQ=255)
                               cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.c.gggcgg                                                                 <  1:314654/101‑5 (MQ=255)
                               cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.C.g.gggccgggggg                                                                  >  1:3083632/1‑91 (MQ=255)
                                aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.c.gggcggc                                                                <  1:1011539/101‑6 (MQ=255)
                                  cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGcggccc                                                               <  1:4918924/101‑1 (MQ=255)
                                  cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCCA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGcggccc                                                               <  2:4492720/101‑1 (MQ=255)
                                    cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGcggcccgg                                                             <  2:3414345/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGcggcccggcc                                                           <  2:43663/101‑1 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GGA.CGGGCGGGCCGGCCg                                                          >  2:2069807/1‑101 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCg                                                          >  1:2145889/1‑101 (MQ=255)
                                         ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGACC.CCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGAt                                                        <  1:2787319/101‑1 (MQ=255)
                                         ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGAt                                                        <  2:3508391/101‑1 (MQ=255)
                                           gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATGAC.AC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATcc                                                      >  2:699078/1‑100 (MQ=255)
                                            cgGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCACC.GGCGGCCCGGCCCATCTg                                                     >  1:5013210/1‑101 (MQ=255)
                                            cgGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTg                                                     >  1:1526449/1‑101 (MQ=255)
                                             gggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCCGCCGATCTgg                                                    >  2:1363505/1‑100 (MQ=255)
                                              ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCg                                                   <  1:159393/101‑1 (MQ=255)
                                              ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCg                                                   <  1:2635195/101‑1 (MQ=255)
                                              ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCg                                                   <  2:4682627/101‑1 (MQ=255)
                                               gTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGGCCGGCCGATCTGCgg                                                  >  1:1524351/1‑101 (MQ=255)
                                                tCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGAGG.TGCTCGCCCGGCAGGG..C.G.C.GCGCACC.GGGGGGCCGGCCGCTTTGCCGc                                                 >  2:3509414/1‑101 (MQ=255)
                                                tCTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGc                                                 <  1:1982896/101‑1 (MQ=255)
                                                 cTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCt                                                >  1:1306090/1‑101 (MQ=255)
                                                 cTGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCCGCCCGTCTGCgggc                                                >  2:2808572/1‑99 (MQ=255)
                                                  tGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCTGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCtt                                               <  2:1593756/101‑2 (MQ=255)
                                                  tGACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGGGGCCCCGCCGGTCTGCGGGTg                                               >  1:802149/1‑101 (MQ=255)
                                                  tGACCCTGGCCGAGGAGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCCGGCGCTCTGCGGccg                                               >  1:4256371/1‑99 (MQ=255)
                                                   gACCCTGGCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTgg                                              <  1:2616883/101‑1 (MQ=255)
                                                      cctggCCGAGGCGCAGCTGGA.ACCCGCGATG.GCGC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACg                                           <  1:4331629/97‑1 (MQ=255)
                                                        tggCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACggg                                         <  1:624457/99‑1 (MQ=255)
                                                          gCCGAGGCGCAGCTGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                                       <  1:4542247/101‑1 (MQ=255)
                                                             gaggcgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                                    <  2:1411706/91‑1 (MQ=255)
                                                              gggcgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                                   <  1:4546943/92‑1 (MQ=255)
                                                              aggcgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGGTCTGCGGCTGGGCGGGGCCCTg                                   >  2:1791275/10‑101 (MQ=255)
                                                              aggcgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                                   <  2:4047142/92‑1 (MQ=255)
                                                               ggcgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                  <  2:4128140/93‑1 (MQ=255)
                                                                gcgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                                 <  1:4143007/94‑1 (MQ=255)
                                                                 cgcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGTCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                                <  2:158647/95‑1 (MQ=255)
                                                                  gcagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGc                               >  2:2657273/6‑101 (MQ=255)
                                                                   cagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGTAGGGCCC.G.G.G.GAA.G.GGCGGTCCGGCCGATCTGCGGCTGGACGGGCTTCTGAAGcc                              >  2:2838301/5‑101 (MQ=255)
                                                                   cagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.GCC.G.GCA.CGGGCGGCCCGGCCGATCTGCGGGTGGGACGGCTCCCTATGcc                              >  2:354160/5‑101 (MQ=255)
                                                                   cagctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGACGcc                              <  2:4279401/97‑1 (MQ=255)
                                                                    agctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCg                             >  1:4955129/4‑101 (MQ=255)
                                                                    agctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCg                             >  1:1813104/4‑101 (MQ=255)
                                                                      ctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGGGGGCTCCTGATGCCggg                           >  2:333202/2‑101 (MQ=255)
                                                                      ctGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCggg                           >  1:3483782/2‑101 (MQ=255)
                                                                       tGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCgggg                          >  2:2793437/1‑100 (MQ=255)
                                                                       tGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc                          <  1:1207695/101‑1 (MQ=255)
                                                                       tGGA.ACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc                          <  2:251222/101‑1 (MQ=255)
                                                                          gaacccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgcgc                        <  2:2145741/97‑1 (MQ=255)
                                                                              ccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                    <  1:4666390/101‑1 (MQ=255)
                                                                              ccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                    <  1:1327610/101‑1 (MQ=255)
                                                                                gcgATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCCGGCGCcgcggc                  >  2:2243740/1‑99 (MQ=255)
                                                                                gcgATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA.c                 <  2:741093/101‑1 (MQ=255)
                                                                                gcgATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA.c                 <  2:1509941/101‑1 (MQ=255)
                                                                                       ggcacggctggCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA.CGCCcg            <  2:1751087/91‑1 (MQ=255)
                                                                                       ggcacggctggCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA.CGCCcg            <  2:1558974/91‑1 (MQ=255)
                                                                                          acggctggCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA.CGCCCgcgc         <  1:1124461/94‑1 (MQ=255)
                                                                                            ggctggCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA.CGCCCGCGCTg       <  2:4954799/96‑1 (MQ=255)
                                                                                            ggctggCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA.CGCCCGCGCTg       <  2:802047/96‑1 (MQ=255)
                                                                                            ggctggCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCCGCTGGACGGGCCCCTGAGGCCGGGCGCCGCGA.AGCCCACGCTg       >  1:4907567/6‑101 (MQ=255)
                                                                                             gcgggCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA.CGCCCGCGCTGa      <  1:440328/97‑1 (MQ=255)
                                                                                              ctggCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGCCGGGCGCCGCGA.CGCCCGCGCTGAt     >  1:2711236/4‑101 (MQ=255)
                                                                                              ctggCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA.CGCCCGCGCTGAt     <  1:1298182/98‑1 (MQ=255)
                                                                                               tggCCGA.GTTGCTCGCCCGGCAGGG..CGG.G.G.GCA.CGGGCGGCCCGGCCGGTCTGCGGCTGGACGGGCTCCTGGTGGCGGGCGCCGGGAGCGCCcccgggggg     >  2:4455044/3‑96 (MQ=255)
                                                                                                 gCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA.CGCCCGCGCTGATCGa  >  1:3449586/1‑101 (MQ=255)
                                                                                                 gCCGA.GTTGCTCGCCCGGCAGGG..C.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA.CGCCCGCGCTGATCGa  <  2:900057/101‑1 (MQ=255)
                                                                                                        |                                                                                                     
GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGA.A.CCGCGATG.G.AA.GGCT.GCCGA.G.TGTTCGCCCGGCAGGG..C.G.C.G.GCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA.CGCCCGCGCTGATCGA  >  NC_014034/3256108‑3256293

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG