Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,532,987 +G 90.4% intergenic (‑6/‑19) RCAP_rcc03340 ← / → RCAP_rcc03341 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,532,9871.→G90.4% 48.0 ‑0.5 ‑0.2177intergenic (‑6/‑19)RCAP_rcc03340/RCAP_rcc03341hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (81/79):  ref base (10/7):  total (91/86)
Fisher's exact test strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.60e-01

Only 100 of 192 total aligned reads displayed.

AGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTC.GGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..GG..CCC.GCCCTTCGATCCAGTGCAGCCAACCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.C.TGCGCGCCCGGCAGGGCCGCCAGATCGTG  >  NC_014034/3532888‑3533079
                                                                                                     |                                                                                                  
  cGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..gg                                                                                                 >  1:3351157/1‑101 (MQ=255)
  cGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGACAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..gg                                                                                                 >  2:1690463/1‑101 (MQ=255)
   ggggAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..ggg                                                                                                >  1:4692923/1‑100 (MQ=255)
    gggAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..gggc                                                                                               >  1:4007766/1‑99 (MQ=255)
     ggAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..Gg..gcc                                                                                            >  2:4114233/1‑101 (MQ=255)
      gAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..Gg..gccc                                                                                           >  1:2715027/1‑100 (MQ=255)
       aaaGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..Gg..gcccg                                                                                          >  1:3051248/1‑99 (MQ=255)
        aggcgccgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..Gg..gcccgg                                                                                         <  1:922412/99‑4 (MQ=255)
          gcggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..Gg..gcccggcc                                                                                       >  2:4151483/1‑96 (MQ=255)
           cggcgCCTTTGGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..Gg..gcccggccc                                                                                      <  2:3793598/101‑7 (MQ=255)
             gcgcCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..Gg..gcccggccctt                                                                                    <  2:4615267/101‑9 (MQ=255)
             gcgcCTGTCGGGCCAGGCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..Gg..gcccggccctt                                                                                    <  2:4513341/101‑9 (MQ=255)
              cgcCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCTGCGCAAAGCTGCG.GG..CCCGGCCCTTc                                                                                    <  2:1040016/101‑1 (MQ=255)
              cgcCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTc                                                                                    <  2:2264553/101‑1 (MQ=255)
               gcCTGTCGGTCCAGTCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCg                                                                                   >  2:4933677/1‑101 (MQ=255)
                 cggTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGGCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGAt                                                                                 <  1:1841458/99‑1 (MQ=255)
                 cTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCCACGCGGCGCAAAGCTGCGGGG..CCG.GCCCTTCGAt                                                                                 >  1:4345315/1‑101 (MQ=255)
                 cTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..GGGACCG.GCCCTTCGAt                                                                                 >  1:2659744/1‑101 (MQ=255)
                  tccgatctcAGCCGCCCGAAAGCAGCCGCCCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATc                                                                                <  1:4565533/93‑1 (MQ=255)
                  tGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATc                                                                                >  1:423892/1‑101 (MQ=255)
                  tGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATc                                                                                >  1:3238209/1‑101 (MQ=255)
                  tGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATc                                                                                >  1:5017413/1‑101 (MQ=255)
                  tGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCAACGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATc                                                                                >  1:4337236/1‑101 (MQ=255)
                  ggccGGCCCAGCCGCCCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATc                                                                                <  2:3774587/98‑1 (MQ=255)
                   gTCGGTCCAGCCGACCGAAAGGAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATcc                                                                               >  1:2874872/1‑101 (MQ=255)
                   gTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATcc                                                                               >  2:1821454/1‑101 (MQ=255)
                     cGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCNNTNCan                                                                             >  1:2271438/1‑100 (MQ=255)
                     cGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAg                                                                             >  1:965403/1‑101 (MQ=255)
                      ggTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCCCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGt                                                                            <  1:1690592/101‑1 (MQ=255)
                        tCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGc                                                                          >  1:2589875/1‑101 (MQ=255)
                        tCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGc                                                                          >  1:2343208/1‑101 (MQ=255)
                        tCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGc                                                                          >  2:2359757/1‑101 (MQ=255)
                         ccAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCa                                                                         >  2:3632071/1‑101 (MQ=255)
                         ccAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCa                                                                         >  2:900412/1‑101 (MQ=255)
                          cAGCCGACCGAAAGCAGCCGCCCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATcagatcgga                                                                        >  2:4565212/1‑93 (MQ=255)
                          cAGCCAACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAg                                                                        <  2:3546258/101‑1 (MQ=255)
                           aGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGc                                                                       >  2:503061/1‑101 (MQ=255)
                             ccgaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCa                                                                     <  1:3666900/101‑1 (MQ=255)
                              cgaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCag                                                                    >  2:744223/1‑100 (MQ=255)
                                accgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGcc                                                                  <  1:1825514/101‑1 (MQ=255)
                                 ccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCg                                                                 <  2:4465588/101‑1 (MQ=255)
                                   gaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTc                                                               >  1:376592/1‑101 (MQ=255)
                                   gaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTc                                                               >  2:3199009/1‑101 (MQ=255)
                                   gaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTc                                                               >  2:4429324/1‑101 (MQ=255)
                                     aaGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCt                                                             <  1:1197165/101‑1 (MQ=255)
                                       gcagcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCg                                                           >  1:3628497/1‑101 (MQ=255)
                                          gcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGAcc                                                        <  2:1501634/101‑1 (MQ=255)
                                            cgcgCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGc                                                      >  1:2187261/1‑101 (MQ=255)
                                              cgcCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCaa                                                    <  2:3237958/101‑1 (MQ=255)
                                               gcCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAc                                                   <  2:3051010/101‑1 (MQ=255)
                                                ccTCGGCCGGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCGGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAcc                                                  <  1:3152373/101‑1 (MQ=255)
                                                ccTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAcc                                                  <  1:2950596/101‑1 (MQ=255)
                                                ccTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAcc                                                  <  1:4934007/101‑1 (MQ=255)
                                                 cTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCg                                                 >  1:557251/1‑101 (MQ=255)
                                                  tCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGt                                                <  2:194692/101‑1 (MQ=255)
                                                   cGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTc                                               <  2:2597509/101‑1 (MQ=255)
                                                   cGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTc                                               >  2:4464553/1‑101 (MQ=255)
                                                   cGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTc                                               >  1:1264933/1‑101 (MQ=255)
                                                    ggCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCg                                              >  2:4360302/1‑101 (MQ=255)
                                                     gCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGc                                             >  1:1114336/1‑101 (MQ=255)
                                                       cAGCGGGGGGTCGGGGGGGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGccc                                           <  1:2175487/101‑1 (MQ=255)
                                                          ccGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGc                                        >  2:3876299/1‑101 (MQ=255)
                                                           cGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGca                                       >  2:2387851/1‑101 (MQ=255)
                                                             ggggTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGcaca                                     >  1:1389211/1‑101 (MQ=255)
                                                                gtcgggggCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAt                                  >  1:2775472/5‑101 (MQ=255)
                                                                gtcgggggCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAt                                  >  2:2128666/5‑101 (MQ=255)
                                                                  cgggggCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cc                               <  2:2822003/99‑2 (MQ=255)
                                                                  cgggggCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cc                               >  1:1513589/3‑100 (MQ=255)
                                                                  cgggggCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cc                               <  1:853552/99‑2 (MQ=255)
                                                                   gggggCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              <  2:3428311/100‑3 (MQ=255)
                                                                   gggggCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              >  1:1022906/2‑99 (MQ=255)
                                                                   gggggCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              >  2:1863844/2‑99 (MQ=255)
                                                                    ggggCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctg                             >  1:619439/1‑98 (MQ=255)
                                                                     gggCGATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctgc                            <  1:3334281/101‑5 (MQ=255)
                                                                         gATCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGCTCC.Tgcgcgc                        >  2:1968118/1‑101 (MQ=255)
                                                                          aTCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGcc                       >  1:3489170/1‑101 (MQ=255)
                                                                          aTCTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGcc                       >  2:866277/1‑101 (MQ=255)
                                                                            cTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCg                     <  1:4402205/101‑1 (MQ=255)
                                                                            cTGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCg                     <  2:100792/101‑1 (MQ=255)
                                                                             tGCGCCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCgg                    <  2:5014079/101‑1 (MQ=255)
                                                                              gcgcCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGc                   >  2:398727/1‑101 (MQ=255)
                                                                              gcgcCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGc                   <  2:4091081/101‑1 (MQ=255)
                                                                                gcCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAg                 >  2:641132/1‑101 (MQ=255)
                                                                                gcCATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAg                 >  1:4838682/1‑101 (MQ=255)
                                                                                 ccATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAgg                <  1:1599472/101‑1 (MQ=255)
                                                                                 ccATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAgg                <  2:1275423/101‑1 (MQ=255)
                                                                                  cATCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAggg               <  2:4739429/101‑1 (MQ=255)
                                                                                   aTCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAGGgc              <  1:1780343/101‑1 (MQ=255)
                                                                                   aTCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAGGgc              <  2:1114222/101‑1 (MQ=255)
                                                                                    tCGCGGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAGGgcc             <  1:503327/101‑1 (MQ=255)
                                                                                     cgcgGCGCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAGGgccg            >  1:1550615/1‑101 (MQ=255)
                                                                                        ggcgCAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAGGGCCGCCa         >  2:2656067/1‑101 (MQ=255)
                                                                                          cgcAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAGGGCCGCCAGa       <  1:4114550/101‑1 (MQ=255)
                                                                                           gcAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAGGGCCGCCAGAt      >  1:4529683/1‑101 (MQ=255)
                                                                                           gcAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAGGGCCGCCAGAt      >  2:1152117/1‑101 (MQ=255)
                                                                                            cAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAGGGCCGCCAGATc     >  2:2293204/1‑101 (MQ=255)
                                                                                            cAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAGGGCCGCCAGATc     <  1:4151800/101‑1 (MQ=255)
                                                                                            cAAAGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGACGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAGGGCCGCCAGATc     >  1:2504378/1‑101 (MQ=255)
                                                                                             aaaGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCC.TGCGCGCCCGGCAGGGCCGCCAGATCg    <  2:1596420/101‑1 (MQ=255)
                                                                                             aaaGCTGCG.GG..CCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGCCGCCCCGCCCAGAT.CATGCGCGCCCGGCAGGGCCGCCAGACCg    >  1:3831924/1‑101 (MQ=255)
                                                                                                     |                                                                                                  
AGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTC.GGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC..GG..CCC.GCCCTTCGATCCAGTGCAGCCAACCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.C.TGCGCGCCCGGCAGGGCCGCCAGATCGTG  >  NC_014034/3532888‑3533079

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG