Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,609,080 +C 98.1% intergenic (+19/‑152) clpB → / → dinB chaperone ClpB/DNA polymerase IV

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,609,0801.→C98.1% 5.2 0.0 ‑0.7259intergenic (+19/‑152)clpB/dinBchaperone ClpB/DNA polymerase IV
Reads supporting (aligned to +/- strand):  new base (108/146):  ref base (2/3):  total (110/149)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.08e-01

Only 100 of 289 total aligned reads displayed.

GCCGGTCCCGACGGGCTGGTGGTCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGT.GGGG.GCT.CT.G.CCCC.C..GCTTCGCTCCCCCCGGGATATTT.G.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGC  >  NC_014034/3608981‑3609173
                                                                                                         |                                                                                                  
 ccgGTCCCGACGGGCTGGTGGTCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.G.c                                                                                                   <  1:1856901/101‑1 (MQ=255)
  cgGTCCCGACGGGCTGGTGGTCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.G.cc                                                                                                  <  2:1870779/101‑1 (MQ=255)
      cccGACGGGCTGGTGGTCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.G.cccc.cc                                                                                             <  2:4298105/101‑2 (MQ=255)
      cccGACGGGCTGGTGGTCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGCGCTGAACTGATA.CGGGAGTCGGGG.GCT.CG.G.cccgcc                                                                                              >  2:1923855/1‑98 (MQ=255)
          gcGGGCTGGTGGGCGGTGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.G.cccc.ccgctt                                                                                         <  2:4627232/100‑6 (MQ=255)
          aCGGGCTGGTGGTCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.G.cccc.ccgctt                                                                                         >  1:2437435/1‑96 (MQ=255)
          aCGGGCTGGGGGTCGGCGGCAAGGTCTCGGTCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.G.cccc.ccgctt                                                                                         <  1:4586181/101‑6 (MQ=255)
           cGGGTTGGTGGTCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTc                                                                                       <  1:794429/101‑1 (MQ=255)
           cGGGCTGGTGGTCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTc                                                                                       >  2:2147321/1‑101 (MQ=255)
           cGGGCTGGTGGTCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTc                                                                                       <  1:4635373/101‑1 (MQ=255)
           cGGGCTGGTGGTCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGT.GGGGCGCTCCT.C.CCCC.C..GCTTc                                                                                       >  2:2731815/1‑101 (MQ=255)
                tggtggTCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTcc                                                                                  <  2:1480428/101‑1 (MQ=255)
                 ggtggtCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTccc                                                                                 >  2:423445/1‑101 (MQ=255)
                  gtggtCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTcccc                                                                                >  2:2856883/1‑101 (MQ=255)
                   tggtCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTccccc                                                                               >  1:1446638/1‑101 (MQ=255)
                    ggtCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTcccccc                                                                              <  1:1609397/101‑1 (MQ=255)
                    ggtCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTcccccc                                                                              <  1:2657313/101‑1 (MQ=255)
                     gtCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCg                                                                             >  2:3875038/1‑101 (MQ=255)
                     gccggcggCAAGGCCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCg                                                                             <  1:3865828/99‑1 (MQ=255)
                      gcggcggCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCgg                                                                            <  2:333526/100‑1 (MQ=255)
                        ggcggcAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGa                                                                          <  1:4657193/101‑1 (MQ=255)
                         gcggcAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGat                                                                         >  1:1292409/1‑101 (MQ=255)
                           ggcAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGatat                                                                       <  2:2590321/101‑1 (MQ=255)
                           ggcAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGatat                                                                       <  1:2797073/101‑1 (MQ=255)
                           ggcAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGatat                                                                       <  2:622107/101‑1 (MQ=255)
                            gcAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATAtt                                                                      <  2:1188664/101‑1 (MQ=39)
                            gcAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGGGCTGAACTGATA.CGGGAGTGGGGG.GCC.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGacttt                                                                      >  1:3842967/1‑97 (MQ=255)
                             cAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATAttt                                                                     >  2:3383012/1‑101 (MQ=39)
                             cAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATAttt                                                                     <  1:745386/101‑1 (MQ=39)
                              agggtccgggCCGGCCGGCGGGCCCAGCCCGACCCGCGCGTGCTGAACTGATAGGGGGGGG.GGGG.GCT.CTCC.CCCC.C..GCTTCGCTCCCCCCGGGATATTT.g                                                                   <  1:3499024/93‑1 (MQ=255)
                                ggTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTT.g.gg                                                                <  2:3761370/101‑1 (MQ=39)
                                ggTCGCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTT.g.gg                                                                <  1:999799/101‑1 (MQ=38)
                                   ctcGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTT.g.gggca                                                             <  2:1236737/101‑4 (MQ=38)
                                   ctcGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTT.g.gggca                                                             >  1:643913/1‑98 (MQ=38)
                                    tcGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTT.g.gggcaa                                                            <  2:2852025/101‑5 (MQ=38)
                                       gccagccaGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGt                                                          <  1:1399967/101‑1 (MQ=38)
                                         tagccagcGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.G.CCCCTC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTaa                                                        <  2:696600/100‑1 (MQ=38)
                                           gccagcGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTaaaa                                                      <  2:2238010/101‑1 (MQ=38)
                                              agcGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCa                                                   >  1:1690956/1‑101 (MQ=37)
                                                ggcgGCCAGACCGAACCGCGCGTGCTGACCTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCAtt                                                 <  2:439586/100‑1 (MQ=25)
                                                cgcgTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCAtt                                                 <  1:946540/101‑1 (MQ=38)
                                                cgcgTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCAtt                                                 <  2:4668367/101‑1 (MQ=38)
                                                 gcgTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTg                                                <  2:4908692/101‑1 (MQ=37)
                                                   gTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGcc                                              >  2:4791393/1‑101 (MQ=37)
                                                   gTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGcc                                              >  2:910114/1‑101 (MQ=37)
                                                   gTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGcc                                              >  1:1094674/1‑101 (MQ=37)
                                                   gTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGcc                                              <  1:2519924/101‑1 (MQ=38)
                                                       aGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCttt                                          >  1:3289112/1‑101 (MQ=37)
                                                        gACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTa                                         <  2:4493336/101‑1 (MQ=38)
                                                        gACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTa                                         <  2:1685249/101‑1 (MQ=38)
                                                        gACCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTa                                         >  1:595332/1‑101 (MQ=37)
                                                         aCCGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTAt                                        <  2:3648804/101‑1 (MQ=38)
                                                          ccGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATc                                       <  2:2203596/101‑1 (MQ=38)
                                                           cGAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCt                                      >  2:4178654/1‑101 (MQ=37)
                                                            gAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCtt                                     >  1:3559239/1‑101 (MQ=37)
                                                            gAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCtt                                     <  1:1208433/101‑1 (MQ=38)
                                                            gAACCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCtt                                     <  1:4904175/101‑1 (MQ=38)
                                                             aaCCGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCCTTATCTTg                                    >  1:4200711/1‑101 (MQ=37)
                                                               ccGCGCGTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGcc                                  <  1:3641499/101‑1 (MQ=38)
                                                                  cgcgTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCa                               <  1:4536235/101‑1 (MQ=38)
                                                                    cgTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGa                             <  1:3895737/101‑1 (MQ=38)
                                                                     gTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGat                            >  2:1334161/1‑101 (MQ=37)
                                                                     gTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGat                            >  2:1857753/1‑101 (MQ=37)
                                                                     gTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGat                            >  1:693251/1‑101 (MQ=37)
                                                                     gTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGat                            <  2:4747563/101‑1 (MQ=38)
                                                                     gTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGat                            >  2:2936478/1‑101 (MQ=37)
                                                                     gTGCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGat                            >  1:1143757/1‑101 (MQ=37)
                                                                       gCTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGatat                          >  1:3219675/1‑101 (MQ=37)
                                                                        cTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGTGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATc                         >  1:2284106/1‑101 (MQ=37)
                                                                        cTGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATc                         <  2:1980283/101‑1 (MQ=37)
                                                                         tGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATcc                        >  2:4068072/1‑101 (MQ=37)
                                                                         tGAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATcc                        >  2:3701460/1‑101 (MQ=37)
                                                                          gAACTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATccc                       >  2:1162252/1‑101 (MQ=37)
                                                                             cTGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCggg                    >  1:882296/1‑101 (MQ=37)
                                                                              tGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCggtg                   >  2:3836113/1‑99 (MQ=25)
                                                                              tGATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCgggg                   >  1:4047478/1‑101 (MQ=37)
                                                                               gATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCggggg                  >  1:3129004/1‑101 (MQ=37)
                                                                               gATA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCggggg                  >  2:1400220/1‑101 (MQ=37)
                                                                                aTA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGGATTGCCCTTTATCTTGCCTAAGATATCCCgggggg                 >  2:772797/1‑100 (MQ=25)
                                                                                 tA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTg                >  1:1495339/1‑101 (MQ=37)
                                                                                 tA.CGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTg                >  1:4210411/1‑101 (MQ=37)
                                                                                    cGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGaa              <  1:1320667/101‑1 (MQ=37)
                                                                                    cGGGAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGaa              <  1:423510/101‑1 (MQ=37)
                                                                                     gggAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAt             <  1:4480307/101‑1 (MQ=37)
                                                                                     gggAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAt             >  1:1613179/1‑101 (MQ=25)
                                                                                     gggAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAt             <  1:4282921/101‑1 (MQ=37)
                                                                                     gggAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAt             <  1:3038769/101‑1 (MQ=37)
                                                                                     gggAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAt             <  1:4703578/101‑1 (MQ=37)
                                                                                      ggAGTGGGGG.GCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAtt            <  2:1752122/101‑1 (MQ=37)
                                                                                       gagtggggg.gCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAttt           <  1:1568100/96‑1 (MQ=37)
                                                                                       gagtggggg.gCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAttt           <  1:4102139/96‑1 (MQ=37)
                                                                                       gagtggggg.gCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAttt           <  2:3241725/96‑1 (MQ=37)
                                                                                        agtggggg.gCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAtttt          <  1:78397/97‑1 (MQ=37)
                                                                                           ggggg.gCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGgc       >  1:3385001/2‑101 (MQ=37)
                                                                                            gggg.gCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATCGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGgcg      >  2:1570945/1‑101 (MQ=37)
                                                                                            gggg.gCT.CT.G.CCCC.CCGGCTTCGCTCCCCCCGGGATATTT.GCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggcca       >  1:3963191/1‑96 (MQ=2)
                                                                                               g.gCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAg   <  2:83346/101‑1 (MQ=38)
                                                                                                 gCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGc  >  1:4333836/1‑101 (MQ=37)
                                                                                                 gCT.CT.GCCCCC.C..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGc  >  2:1118884/1‑101 (MQ=37)
                                                                                                 gCT.CT.G.CCCC.CCGGCTTCGCTCCCCCCGGGATATTT.GCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggccaaagg   >  2:3666806/1‑92 (MQ=2)
                                                                                                         |                                                                                                  
GCCGGTCCCGACGGGCTGGTGGTCGGCGGCAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATA.CGGGAGT.GGGG.GCT.CT.G.CCCC.C..GCTTCGCTCCCCCCGGGATATTT.G.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGC  >  NC_014034/3608981‑3609173

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG