Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 580,930 +C 2.3% intergenic (+51/‑320) RCAP_rcc00541 → / → RCAP_rcc00542 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034580,9301.→C 2.3% 4.9 ‑1.2 ‑1.4176intergenic (+51/‑320)RCAP_rcc00541/RCAP_rcc00542hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (54/118):  total (54/122)
Fisher's exact test strand distribution p-value = 3.14e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.63e-02

Only 100 of 213 total aligned reads displayed.

CCCTGATCCGGGGGGAAACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCC  >  NC_014034/580830‑581029
                                                                                                         |                                                                                                    
   tGATCCGGGGGGAAACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAAAT.CA.ccc                                                                                                   >  2:2181754/1‑101 (MQ=255)
     aTCCGGGGGGAAACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.ccccc                                                                                                 <  1:3428387/101‑1 (MQ=255)
     aTCCGGGGGCAAACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.ccccc                                                                                                 <  2:3442988/101‑1 (MQ=255)
      tCCGGGGGGAAACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGGGGTGCCCCCCCCTTTTGCGCGGC.CCG.AAATT.CC.ccccc                                                                                                 >  1:2550763/1‑101 (MQ=255)
       ccGGGGGGAAACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGTTG.CCCCCTCTTTCC.GCTGCGCCG.GAATT.TA.TCCCCgg                                                                                               >  2:455492/1‑101 (MQ=255)
       ccGGGGGGAAACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCgg                                                                                               <  2:37716/101‑1 (MQ=255)
        cGGGGGGAAACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCggg                                                                                              <  1:1987812/101‑1 (MQ=255)
          gggggAAACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGat                                                                                            >  2:2127318/1‑101 (MQ=255)
            gggAAACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGatat                                                                                          <  1:4700625/101‑1 (MQ=255)
            gggAAACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGatat                                                                                          <  1:1481357/101‑1 (MQ=255)
             ggAAACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATAtt                                                                                         <  1:2168066/101‑1 (MQ=255)
             ggAAACCGCGCGTGACAAGGTGTGCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATAtt                                                                                         <  2:4157951/101‑1 (MQ=255)
               aaaCCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGGTATTTg                                                                                       >  2:2911802/1‑101 (MQ=255)
               aaaCCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTg                                                                                       <  2:51369/101‑1 (MQ=255)
               aaaCCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTg                                                                                       <  1:1663621/101‑1 (MQ=255)
               aaaCCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTg                                                                                       <  2:3685244/101‑1 (MQ=255)
               aaaCCGAGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTg                                                                                       <  1:813723/101‑1 (MQ=255)
                aaCCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTgg                                                                                      >  2:2833468/1‑100 (MQ=255)
                 aCCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAg                                                                                     <  1:224713/101‑1 (MQ=255)
                  ccGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAgg                                                                                    <  2:3990126/101‑1 (MQ=255)
                    gcgcgTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGGGCTG.CCCCCCCTTTTC.GCTTCGCCGCAAATTACC.CCCCCGGGATTTTTggga                                                                                    >  1:1758607/1‑98 (MQ=255)
                    gcgcgTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGCGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCa                                                                                  <  1:4244393/101‑1 (MQ=255)
                      gcgTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAg                                                                                <  2:4726220/101‑1 (MQ=255)
                       cgTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGa                                                                               <  1:4329401/101‑1 (MQ=255)
                        gTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGAt                                                                              <  2:1351896/101‑1 (MQ=255)
                        gTGACAAGGTGTTACGCCTCGCTTGAGGCGGCCCGGGCCGGGGGCGCTG.CCCCCTCTTTCC.GCTGAGCCG.CCATT.CC.CACCAGGGCTATTTGAGGCAAGaa                                                                              >  2:2415237/1‑100 (MQ=25)
                          gACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGGGGTG.CCCCCTCTTTTC.GCTGGGCCG.AAATT.CA.ACCCCCGGATATTTGAGGCAAGAtca                                                                            >  2:4780502/1‑99 (MQ=255)
                          gACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGAT.aa                                                                           <  2:1908191/101‑1 (MQ=255)
                          gACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGAT.aa                                                                           <  1:1619701/101‑1 (MQ=255)
                          gACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGAT.aa                                                                           <  1:1720600/101‑1 (MQ=255)
                          gACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.ACTGCGCTG.AAATT.CC.CCCCCGGGATATTTGAGGCAAGAT.aa                                                                           <  2:1741225/101‑1 (MQ=255)
                          gACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCC.GCTGCGCCG.CAATT.CC.CCCCCGCGCTATTTGTGGCaaaacaa                                                                            >  2:4917379/1‑96 (MQ=25)
                           aCAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CT.CCCCCGGGATATTTGAGGCAAGAT.aaa                                                                          <  2:1299888/101‑2 (MQ=255)
                             aaGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCCG.CCCCCCCTTTCG.GCTGCGCCG.CAATT.CA.CCCCCGGGGGATTTGAGGCAAGAT.aaacg                                                                        >  1:412851/1‑98 (MQ=38)
                              aGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGAT.aaacgg                                                                       >  2:3236746/1‑97 (MQ=255)
                                     ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGGTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                 <  1:4352044/101‑1 (MQ=255)
                                     ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                 <  2:4426866/101‑1 (MQ=255)
                                     ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                 <  2:1468936/101‑1 (MQ=255)
                                      cGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAt                                                                <  2:2550067/101‑1 (MQ=255)
                                       gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATc                                                               <  1:4425145/101‑1 (MQ=255)
                                       gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATc                                                               <  1:4757738/101‑1 (MQ=255)
                                          tCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CC.CCCCCCGGGTATTTGAGGCAAGATAAACGGGCGCCTATCAcc                                                            >  1:2407286/1‑101 (MQ=25)
                                              ttGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTgg                                                        >  1:1531128/1‑101 (MQ=255)
                                               tGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CC.CCCCCGGGATATTTGAGGCCAGATAAACGGGCGCCTATCACCTTGGt                                                       >  1:2129768/1‑101 (MQ=25)
                                                 aGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGc                                                     >  2:832280/1‑101 (MQ=255)
                                                  ggcggcCCGGGCAGGGGGCGCTG.CCCCCTCTTTTG.GCTGCGCCG.AAATT.TA.CCCCCGGGATATTTTAGGCAAGATAAACGGGCGCCTATCACCTTGGTGcc                                                    >  1:4812793/1‑100 (MQ=21)
                                                  ggcggcCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGAAGTTTGAGGCAAGATAAACGGGGGCCTATCACCTTGGTGCa                                                    >  2:3123552/1‑101 (MQ=25)
                                                    cggcCCGGGCAGGGGGCGCTG.CCCCCTCTTTTG.GCTGGGCCG.AAATT.TA.CCCCCGGGGTATTTTAGGCAAGATAAACAGGCGCCTATCACCTTGGGGCcaa                                                  >  1:59108/1‑101 (MQ=18)
                                                    cggcCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCaaa                                                  <  2:2475262/101‑1 (MQ=255)
                                                    cggcCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCaaa                                                  <  2:3736550/101‑1 (MQ=255)
                                                     ggcCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAat                                                 <  2:2709816/101‑1 (MQ=255)
                                                          gggCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCg                                            <  2:2808298/101‑1 (MQ=255)
                                                          gggCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCg                                            <  1:3578657/101‑1 (MQ=255)
                                                            gCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGTGCCTATCACCTTGGTGCAAATATCCggg                                          <  1:2980273/101‑1 (MQ=255)
                                                            gCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCggg                                          <  1:5009701/101‑1 (MQ=255)
                                                             cAGGGGGCGCTG.CCCCCTCTTTTG.GCTGCGCCG.AAATT.CA.CCCCCCGGGTATTTGAGGCAAGATAAAAGGGGGCCCATCACCCTGGTGCCAATAACCgggg                                         >  2:86290/1‑101 (MQ=17)
                                                             cAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgggg                                         >  2:4984320/1‑101 (MQ=255)
                                                              aGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCggggg                                        >  1:4625452/1‑101 (MQ=255)
                                                               gggggCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCAGCTTGGTGCAAATATCCgggggg                                       >  2:848348/1‑101 (MQ=255)
                                                                tccgatcTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGt                                      <  1:3655851/95‑1 (MQ=25)
                                                                ggggCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGGGCCTATCACCTTGGTGCAAATATCCggggggg                                      >  2:77383/1‑100 (MQ=255)
                                                                ggggCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGt                                      >  2:3038443/1‑101 (MQ=255)
                                                                  ggCGCTG.CCCCCTCTTGCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGa                                    <  2:264896/101‑1 (MQ=255)
                                                                     gcTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCCGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGGGAggg                                 >  2:3532231/1‑100 (MQ=38)
                                                                        g.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                              <  1:1900393/101‑1 (MQ=255)
                                                                        g.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                              <  1:732097/101‑1 (MQ=255)
                                                                        g.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGGGAGGCCGa                              >  2:3620194/1‑101 (MQ=255)
                                                                        g.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGGGAGGCCGa                              >  1:3194913/1‑101 (MQ=255)
                                                                        g.CCCCCTCTTTCG.GCCGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                              <  1:1199307/101‑1 (MQ=255)
                                                                        g.CCCCCTCGTTCG.GGTGGGCCG.CAACT.CC.CCCCCGGGATATTTGAGGCCAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                              <  1:2517012/101‑1 (MQ=25)
                                                                           ccccTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                            <  2:1063749/101‑1 (MQ=255)
                                                                           ccccTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                            <  2:2863793/101‑1 (MQ=255)
                                                                               tctTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                        <  1:1774647/101‑1 (MQ=255)
                                                                                ctTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGa                       >  1:1032956/1‑101 (MQ=255)
                                                                                  ttCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                     >  2:3103399/1‑101 (MQ=255)
                                                                                  ttCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                     <  2:1766351/101‑1 (MQ=255)
                                                                                     g.gCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAggggg                  >  1:2091462/1‑101 (MQ=255)
                                                                                        cTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                >  1:4169521/1‑101 (MQ=255)
                                                                                        cTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                <  1:3363887/101‑1 (MQ=255)
                                                                                         tGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAg               <  2:1873856/101‑1 (MQ=255)
                                                                                          gcgcCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgc              <  1:4463664/101‑1 (MQ=255)
                                                                                           ttcggccaaTT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGGGGTTTATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcg             <  2:205968/94‑1 (MQ=37)
                                                                                           ttcggccaaTT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGGGGTTTATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcg             <  2:4002329/94‑1 (MQ=37)
                                                                                           cgcCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg             <  2:3874545/101‑1 (MQ=255)
                                                                                            tcggccaaTT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGGGGTTTATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcgc            <  1:1875534/95‑1 (MQ=37)
                                                                                              cG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccc          >  1:3876859/1‑101 (MQ=255)
                                                                                               g.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATGCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc         >  1:3518019/1‑101 (MQ=255)
                                                                                               g.AAATT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc         >  1:1952724/1‑101 (MQ=255)
                                                                                                 aaaTT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc        <  2:358100/101‑1 (MQ=255)
                                                                                                  aaTT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt       >  1:2842200/1‑101 (MQ=255)
                                                                                                  aaTT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt       <  1:518923/101‑1 (MQ=255)
                                                                                                   aTT.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt      <  2:1623281/101‑1 (MQ=255)
                                                                                                    tt.CA.CCCCCGGGATATTTGAGGCAAGATAAACGAGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCttt     <  2:2516032/101‑1 (MQ=255)
                                                                                                     t.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt    >  2:295541/1‑101 (MQ=255)
                                                                                                     t.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt    >  1:4764339/1‑101 (MQ=255)
                                                                                                     t.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt    <  2:2378174/101‑1 (MQ=255)
                                                                                                     t.CA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCttta    >  1:851186/1‑100 (MQ=255)
                                                                                                       cA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc   >  1:2489010/1‑101 (MQ=255)
                                                                                                        a.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc  <  1:2428212/101‑1 (MQ=255)
                                                                                                        a.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc  >  2:1870794/1‑101 (MQ=255)
                                                                                                         |                                                                                                    
CCCTGATCCGGGGGGAAACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTG.CCCCCTCTTTCG.GCTGCGCCG.AAATT.CA.CCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCC  >  NC_014034/580830‑581029

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG