Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	774,643	+C	100%	intergenic (‑97/+23)	oppB1 ← / ← oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	774,643	1	.→C	100.0%	79.4	21	intergenic (‑97/+23)	oppB1/oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (17/4):  ref base (0/0):  total (17/4)

CGGGGGGGCGCCGGGCCGCA......GCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCC.GG.T.CGA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAC  >  NC_014034/774585‑774728                                                                     |                                                                                      cGGGGGGGCGCCGGGCCGCAGGCCCGGCGCCCCCCCGCCCGGGGCGGACCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCcg                                                     >  1:4468860/1‑101 (MQ=255)                   caggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGtt                                   >  1:88244/9‑101 (MQ=255)                   caggcccggcgcCCCCCCGCCCGCGTCGGACCGGCCCTGCC.GGCC.CGACCCCACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGGCGGCCACggt                                   >  2:2452122/9‑99 (MQ=255)                    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCCGCCGGG.C.CGACCCTACCGGCTCTTATCCTGCGATCACTTCGCCCGCGACCCGACGGCCACGTTc                                  >  1:4561874/8‑101 (MQ=255)                     ggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTcc                                 >  1:1432018/7‑101 (MQ=255)                       cccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGGCGGCCACGTTCCAg                               >  2:4668931/5‑101 (MQ=255)                          ggcgcCCCCCCGCCCGGGACGGACCGGCCCTGCC.CG.TCCGACCCTACGGTCTCTTATCCTGCGATCCCCTCGCCCGCGACCCGTCGGCCCCGTTCCAATAt                            >  2:2109406/2‑101 (MQ=255)                                      cccgggtcggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAg                >  2:2366646/11‑101 (MQ=255)                                          ggtcggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGt            >  2:3877344/7‑101 (MQ=255)                                          ggtcggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGt            >  2:3596841/7‑101 (MQ=255)                                           gtcggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGtt           >  1:235576/6‑101 (MQ=255)                                           gtcggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGtt           >  2:3021290/6‑101 (MQ=255)                                           gtcggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGtt           >  1:3013283/6‑101 (MQ=255)                                            tcggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTc          >  2:3808140/5‑101 (MQ=255)                                             cggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  1:3469827/4‑101 (MQ=255)                                              ggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt        >  2:3798703/3‑101 (MQ=255)                                                aCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtct      <  1:417746/101‑1 (MQ=255)                                                 ccGGCCCTGCC.GG.TCCGACCCTGCGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtctc     <  1:2561524/101‑1 (MQ=255)                                                 ccGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtctc     <  1:1850282/101‑1 (MQ=255)                                                   ggCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGa   >  2:3183964/1‑101 (MQ=255)                                                   ggCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGa   >  1:2572980/1‑101 (MQ=255)                                                   gccccTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGa   <  2:4468541/99‑1 (MQ=255)                                                    gCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCgcc  >  1:1245528/1‑99 (MQ=255)                                                    ccccTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCgcc  <  2:1245407/100‑3 (MQ=255)                                                    ccccTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc  <  2:4221264/100‑1 (MQ=255)                                                                     |                                                                                      CGGGGGGGCGCCGGGCCGCA......GCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCC.GG.T.CGA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAC  >  NC_014034/774585‑774728 Base quality scores:  ATCG  < 3 ≤  ATCG  < 14 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG