Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	900,802	+CC	100%	intergenic (‑131/‑156)	ldh ← / → ftsW	L‑lactate dehydrogenase/cell division protein FtsW

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	900,802	1	.→C	100.0%	151.9	63	intergenic (‑131/‑156)	ldh/ftsW	L‑lactate dehydrogenase/cell division protein FtsW
Reads supporting (aligned to +/- strand):  new base (56/6):  ref base (0/1):  total (56/7)
*	NC_014034	900,802	2	.→C	100.0%	151.9	63	intergenic (‑131/‑156)	ldh/ftsW	L‑lactate dehydrogenase/cell division protein FtsW
Reads supporting (aligned to +/- strand):  new base (56/6):  ref base (0/1):  total (56/7)

CAACCCCGTTTCCG.CGCGG.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GC...CGCGGA..G.....CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCAGA  >  NC_014034/900724‑900874                                                                                          ||                                                                            cAACCCCGTTTCCGCCGCGGCCGCCCCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGcccc                                                           >  2:161181/1‑101 (MQ=16)  acccccGTCCCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCg                                                          <  2:504201/99‑1 (MQ=12)       cGTTTCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCt                                                     >  2:696825/1‑101 (MQ=16)         tttCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGc                                                   >  1:348825/1‑101 (MQ=16)          ttCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGcc                                                  >  1:3358082/1‑101 (MQ=16)          ttCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGcc                                                  >  1:3654726/1‑101 (MQ=16)          ttCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGcc                                                  >  1:2577190/1‑101 (MQ=16)          ttCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGGCGCCCCCGCCCCGcc                                                  >  1:2542278/1‑101 (MQ=12)            ccgccgggcccgccccTTTGCGCCAGCCGCGGTTTCCCTGGGTGCAAATCCGCCGGGGGGG...GG...GGGGGG..GGACCCCCCGCGGGTCGCCCCGGCCCTGCCgg                                                <  2:1424475/91‑1 (MQ=2)              gccgcgGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCgggg                                              >  1:4011382/3‑101 (MQ=18)                 gcggccgccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGa                                           >  2:3805512/6‑101 (MQ=18)                   ggccgccgtCTTGCCGCAGCCCCGGTTTTCCTTGTTGCAAATACGCCCGGGGGT...GCGGGGGCGGACCC.....CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGaaa                                         <  1:768977/98‑1 (MQ=12)                   ggccgccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGaaa                                         >  1:4734893/4‑101 (MQ=18)                   ggccgccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGaaa                                         >  1:4430520/4‑101 (MQ=18)                     ccgccgcTTTGCCGCAGCCCCGGTTTTCCTTGGGGCAAATACGCCCGGGGGG...GCGGGGGCGGG..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGc                                       <  1:3399635/100‑1 (MQ=18)                       gccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGccc                                     >  2:1359774/1‑101 (MQ=21)                        ccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGcccc                                    >  1:4249646/1‑101 (MQ=21)                         cgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGcagatcggaag                                   >  1:3741167/1‑91 (MQ=18)                          gcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcg                                  >  2:1505249/1‑101 (MQ=18)                           cTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgc                                 <  2:1249050/101‑1 (MQ=18)                           cTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgc                                 >  1:1343348/1‑101 (MQ=18)                           cTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgc                                 >  1:1126520/1‑101 (MQ=18)                            tttGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgcg                                >  2:2863662/1‑101 (MQ=18)                            tttGCCGCAG.CCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgcgc                               >  2:3310668/1‑101 (MQ=17)                               gCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCgcgcgc                             >  2:260730/1‑101 (MQ=18)                               gCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCgcgcgc                             >  1:3087652/1‑101 (MQ=18)                               gCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCgcgcgc                             >  1:3075963/1‑101 (MQ=18)                                 cGCCGCCCCGGTGTTCCTTGGTGCAAATGCGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccg                           <  2:623944/101‑1 (MQ=17)                                 cGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccg                           >  1:2774610/1‑101 (MQ=18)                                 cGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCCcgccg                           >  2:1540981/1‑101 (MQ=18)                                  gCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccgc                          >  2:2171180/1‑101 (MQ=18)                                  gCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGGCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccgc                          >  2:1735374/1‑101 (MQ=21)                                   cAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCcgcg                         >  2:986610/1‑101 (MQ=18)                                   cAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCcgcg                         >  2:2209175/1‑101 (MQ=18)                                    aGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCgg                        >  2:2109368/1‑101 (MQ=18)                                      ccccGGTTTTCCTTGGTGCAAATCCGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGcc                      <  1:3131944/101‑1 (MQ=18)                                         cGGTTTTCCTTGGTGCAAATCCGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCgg                   <  2:2437477/101‑1 (MQ=18)                                         cGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCgg                   >  1:2658809/1‑101 (MQ=18)                                          ggTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGTCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGc                  >  1:4347101/1‑101 (MQ=18)                                          ggTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGc                  >  1:2524660/1‑101 (MQ=18)                                          ggTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGc                  >  2:3534451/1‑101 (MQ=18)                                          ggTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCCCGCGCCGCGGCCCGGc                  >  2:3481631/1‑101 (MQ=18)                                           gTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCa                 >  2:1704401/1‑101 (MQ=18)                                            ttttCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaa                >  1:2615608/1‑101 (MQ=18)                                             tttCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaaa               >  1:1490589/1‑101 (MQ=18)                                             tttCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaaa               >  2:1285925/1‑101 (MQ=18)                                             tttCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaaa               >  2:1041277/1‑101 (MQ=18)                                             tttCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGCGGGCCCGGCCCCGCCGGGGCGAAAGCCCCCGCGCGCCCCGGCCCGGCaaa               >  2:4499293/1‑101 (MQ=16)                                              ttCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaaaa              >  2:1538719/1‑101 (MQ=18)                                              ttCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGGCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaaaa              >  1:1073468/1‑101 (MQ=25)                                               tCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAg             >  2:4896549/1‑101 (MQ=18)                                               tCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGCCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAg             >  1:2627697/1‑101 (MQ=21)                                                 cTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGct           >  1:4724140/1‑101 (MQ=18)                                                   tGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGctct         >  1:1790722/1‑101 (MQ=18)                                                    ggTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTg        >  1:1902627/1‑101 (MQ=18)                                                      tGCAAATACGCCCGGGGGTCGGGG...GGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGt      <  2:679202/101‑1 (MQ=18)                                                      tGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGt      >  1:4272611/1‑101 (MQ=18)                                                      tGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGt      >  1:2585050/1‑101 (MQ=18)                                                       gCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTc     >  2:1484401/1‑101 (MQ=18)                                                       gCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTc     >  2:1048734/1‑101 (MQ=18)                                                        cAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCa    >  2:603868/1‑101 (MQ=18)                                                          aaTACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCaga  >  2:2718226/1‑101 (MQ=18)                                                          aaTACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCaga  >  2:1154767/1‑101 (MQ=18)                                                          aaTACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCaga  >  2:4034009/1‑101 (MQ=18)                                                          aaTACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCaga  >  2:4764751/1‑101 (MQ=18)                                                                                          ||                                                                            CAACCCCGTTTCCG.CGCGG.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GC...CGCGGA..G.....CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCAGA  >  NC_014034/900724‑900874 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG