Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 901,415 +G 2.2% coding (458/1170 nt) ftsW → cell division protein FtsW

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034901,4151.→G 2.2% 4.6 ‑3.7 ‑0.0180coding (458/1170 nt)ftsWcell division protein FtsW
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (69/107):  total (73/107)
Fisher's exact test strand distribution p-value = 2.57e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.80e-01

Only 100 of 219 total aligned reads displayed.

TGGATCTCGCTCGGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTCGGCCAGGCCTCGCTGATCCTCTTT  >  NC_014034/901315‑901515
                                                                                                     |                                                                                                        
 ggATCTCGCTCGGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.g                                                                                                         >  2:1705000/1‑101 (MQ=255)
 ggATCTCGCTCGGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGGGGC.g                                                                                                         >  1:576529/1‑101 (MQ=255)
     ctcgctcgGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgc.c                                                                                                    <  1:3564529/101‑1 (MQ=255)
     ctcgctcgGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgc.c                                                                                                    <  1:1638782/101‑1 (MQ=255)
      tcgctcgGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgc.cc                                                                                                   <  2:1524703/101‑1 (MQ=255)
      tcgctcgGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgc.cc                                                                                                   <  2:2169486/101‑1 (MQ=255)
      tcgctcgGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgc.cc                                                                                                   <  2:4405756/101‑1 (MQ=255)
        gctcgGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.gggggcgc                                                                                                  >  2:3456328/1‑96 (MQ=255)
        gctcgGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgg.cccc                                                                                                 >  1:704222/1‑99 (MQ=255)
         ctcgGCTTCGCCTCTTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgc.ccg.cc                                                                                               <  2:4755540/101‑1 (MQ=255)
          tcgGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgc.ccg.ccc                                                                                              <  2:1258137/101‑1 (MQ=255)
          tcgGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgc.ccg.ccc                                                                                              <  1:935425/101‑1 (MQ=255)
          tcgGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgc.ccg.ccc                                                                                              <  1:3306884/101‑1 (MQ=255)
          gatctttCGCCTCGTTCCAGCCTACGTAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgc.ccg.ccc                                                                                              <  2:4719772/96‑1 (MQ=255)
           cgGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgg.ccc.cccc                                                                                             >  1:1313790/1‑100 (MQ=255)
           cgGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgg.ccc.cccc                                                                                             >  2:2013462/1‑100 (MQ=255)
           cgGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgg.ccc.cccc                                                                                             >  1:4038751/1‑100 (MQ=255)
           cgGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGgc.ccg.cccc                                                                                             >  2:2442951/1‑100 (MQ=255)
            ggCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.gg                                                                                           <  1:3646025/101‑1 (MQ=255)
               ttCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCG.CCC.Cggc.g                                                                                       >  2:856553/1‑101 (MQ=255)
                tCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCC.ACC.Cggc.gg                                                                                      >  1:2016249/1‑101 (MQ=255)
                 cGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCC.CCC.Cggg.ggg                                                                                     >  2:3019142/1‑100 (MQ=255)
                    cTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCttt                                                                                  <  1:1648377/101‑1 (MQ=255)
                       gTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCG.CCC.Cggc.gggcttttt                                                                               >  1:3148253/1‑94 (MQ=255)
                       gTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCC.CCC.AGGG.GGCGTTTtt                                                                               >  2:1371195/1‑101 (MQ=255)
                       gTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGCGGC.GGGCGCCC.CCC.GGGG.GGCtttgt                                                                                >  1:1190609/1‑99 (MQ=255)
                        ttCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTc                                                                              <  1:1095554/101‑1 (MQ=255)
                          ccAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCC.CCC.Cggc.ggggctttttct                                                                            >  2:3781871/1‑91 (MQ=255)
                           cAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCtt                                                                           <  2:1928103/101‑1 (MQ=255)
                           cAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCtt                                                                           <  2:240313/101‑1 (MQ=255)
                            aGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGCGGGGC.CCC.CCC.GGGG.GGGTTTTTTCTtt                                                                           >  1:1031029/1‑101 (MQ=255)
                              ccTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCG.CCC.CGGC.GGCCTTTTTTCttttt                                                                        >  1:2382896/1‑98 (MQ=255)
                              ccTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCAt                                                                        <  2:3428170/101‑1 (MQ=255)
                               cTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATc                                                                       <  1:329438/101‑1 (MQ=255)
                                 tcgtatttCCTCATGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCTCCTCGCTTGAAGTTGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCt                                                                     <  2:1622843/95‑1 (MQ=255)
                                 tCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCG.CCC.CGGC.GGGTTTATTctcttctccc                                                                     >  2:4078775/1‑92 (MQ=255)
                                 tCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCt                                                                     <  2:1428718/101‑1 (MQ=255)
                                 tCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCt                                                                     <  2:1510359/101‑1 (MQ=255)
                                  cGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCC.CCCGGGGG.GGCTTTTTTCTTTCTTctc                                                                     >  1:4887768/1‑99 (MQ=255)
                                   ggAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGCGGGGC.CCC.CCC.GGGG.GGGTTT.TTCCtttctccccc                                                                   >  2:3543502/1‑93 (MQ=255)
                                   ggAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCGCCCC.GGGG.GGCTTTTTTCTttttcctca                                                                    >  2:3741802/1‑94 (MQ=255)
                                   ggAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCa                                                                   <  2:3272473/101‑1 (MQ=255)
                                   ggAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCC.GCC.CGGC.GGCGTTTTTCCttttttcctc                                                                   >  2:4126521/1‑93 (MQ=255)
                                    gAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.CGGC.GGCCTTTTTTCTTTATcccccc                                                                  >  1:4895430/1‑97 (MQ=255)
                                     aaTTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCG.CCC.GGGC.GGGGTTATTTCtttcccctcccc                                                                 >  1:2893552/1‑91 (MQ=255)
                                     aaTTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCG.CCC.CGGG.GGGGTTTTTTCTTCCTTCTCAcc                                                                 >  2:1784603/1‑101 (MQ=255)
                                             aaGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGAt                                                         <  1:1265883/101‑1 (MQ=255)
                                              aGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGCGGGGC.CCC.CCC.GGCG.GGCTTTTTTCTTTC.TCCTCCCCCCCCTGGtc                                                        >  2:3685773/1‑101 (MQ=255)
                                              aGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGAtc                                                        <  1:856655/101‑1 (MQ=255)
                                              aGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGAtc                                                        <  1:68002/101‑1 (MQ=255)
                                              aGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGAtc                                                        <  2:3914020/101‑1 (MQ=255)
                                              aGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGAtc                                                        <  2:1790261/101‑1 (MQ=255)
                                              aGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCC.CCC.GGGG.GGCGTTTTTTCTTCCTTCCCACCccgccggtc                                                        >  2:4990242/1‑96 (MQ=255)
                                                cccGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGAtcgt                                                      <  2:189586/101‑1 (MQ=255)
                                                 ccGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.CGGG.GGCGTTTTTTCTTTATCCTCCCCCCCCCGAtcggc                                                     >  1:1048963/1‑99 (MQ=255)
                                                 ccGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.CGGCGGGCTTTTTCTCTTC.TCCTCACCCCGCTGAtcggc                                                     >  2:1192274/1‑99 (MQ=255)
                                                    gCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCG.CCC.CGGG.GGGGTTTTTTCTTTTTCCTCCCCCCGCCGCTCgtcgtc                                                  >  2:1059892/1‑101 (MQ=255)
                                                     cTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCa                                                 <  1:3456593/101‑1 (MQ=255)
                                                      ttCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCG.CCC.CGGC.GGCGTTTTTCCTTCCTCCTCCCCCCCCCGGTCGTCGTCAc                                                >  2:4516704/1‑101 (MQ=255)
                                                       tCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCAcc                                               <  1:1905205/101‑1 (MQ=255)
                                                        cGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCAccc                                              <  2:3652637/101‑1 (MQ=255)
                                                         gTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCGCCCC.GGGG.GGCTTT.TTTCTTCATTCCCACCCCCCCCATCGTCGCCAcccc                                             >  1:2142890/1‑100 (MQ=255)
                                                         gTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCATCATCCTCACCGCGCTGATCGTCGTCACCCt                                             <  1:4473531/101‑1 (MQ=255)
                                                           cATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCGCCCC.GGGG.GGCTTT.TTCCTTCATCCCCACCCCGCCGATCGCCGGCCCCCCgg                                           >  2:2731632/1‑101 (MQ=255)
                                                           cATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCG.CCC.CGGC.GGGTTTTTTTCTTCCTCCTCACCCCGCTGATCGTCGTCAcccccg                                           >  2:4227888/1‑98 (MQ=255)
                                                           cATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCC.CCC.GCGG.CGCTTTATTCCCTTATCCTCACCCCGCGCGTCGTCGTCACCCTgg                                           >  2:3031233/1‑101 (MQ=255)
                                                           cATCGTCTCGGCCTGGGTCATGGCCGCCGCGCTGGAAGTGGC.GGGG.CCC.CCC.CGGC.GGCTTTTTTTCTTCATCCTCACCCCCCCGGTCGTCGCCCCCCCgg                                           >  1:4413133/1‑101 (MQ=255)
                                                            aTCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGc                                          <  2:2843534/101‑1 (MQ=255)
                                                             tCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCa                                         <  2:704133/101‑1 (MQ=255)
                                                             tCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCa                                         <  1:972821/101‑1 (MQ=255)
                                                              cGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCAc                                        <  1:2598188/101‑1 (MQ=255)
                                                              cGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.CGGC.GGCGTTATTTCTTCAACCCCACCCCCCTCCTCCTCGTCCCCCTGGCAc                                        >  1:1800853/1‑101 (MQ=255)
                                                              cGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCC.CCC.CGGC.GGCTTTATTTCTTCATCCTCCCCCCCCCGATCGTCGTCACCCCGGCAc                                        >  1:3343862/1‑101 (MQ=255)
                                                                tctcGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTg                                      <  2:4558851/101‑1 (MQ=255)
                                                                tctcGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.CGGC.GGCGTTTTTTCTTCATCCTCCCCCCGCTGATCGTCGGCACCCCGGCCCTg                                      >  1:46356/1‑101 (MQ=255)
                                                                   cGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGGGTTTTTTCTTTTTCCTCACCCCGCTGATCGTTGTCCCCCCCGCAcccccg                                   >  1:746502/1‑96 (MQ=255)
                                                                    ggCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.CGGC.GGGGTTATTCCTTCATCCTCCCCCCGCTGCTCGTCGTCACCCTGGCACTGCacc                                  >  2:2529736/1‑99 (MQ=255)
                                                                      ccTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCC.CCC.GGGC.GGCTTTTTTTCTTCATTCTCCCCGCGCTGGTCGGGGTGCCCCCGGCACTCCAGccc                                >  1:2690290/1‑101 (MQ=255)
                                                                         ggTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGAc                             <  1:4397951/101‑1 (MQ=255)
                                                                         ggTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.CGGC.GGGTTTTTTTCTTTATCCTCACCCCGCCGATCGTCGTCACCCCGGCCCTGCAGCCCgcc                             >  1:2547090/1‑99 (MQ=255)
                                                                          gTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACt                            <  1:3661938/101‑1 (MQ=255)
                                                                            tCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTc                          <  1:3782156/101‑1 (MQ=255)
                                                                            tCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTc                          <  1:2246474/101‑1 (MQ=255)
                                                                              aTGGCCGCCGCGCTGGAAGTGGC.GGGC.CCC.CCC.GGGC.GGCTTTTTTTCTTTATCCTCACCCCGCCGATCGTCGTCACCCCGGCACTGCAGCCCCACTTccg                        >  1:4921952/1‑99 (MQ=255)
                                                                                  ccgccgCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTCGGCCAg                    <  2:573936/101‑1 (MQ=255)
                                                                                  ccgccgCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTCGGCCAg                    <  1:3608102/101‑1 (MQ=255)
                                                                                    gccgcGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTTTTTCTTCATCCTCCCCCCGCTGATCGTCGGCCCCCCGGGCCTGCCGCCCGCCTTCGGCCCGGc                  >  2:668597/1‑101 (MQ=255)
                                                                                     ccgcGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTCGGCCTGGcc                 <  2:4275632/101‑1 (MQ=255)
                                                                                     ccgcGCTGGAAGTGGC.GGGC.CCC.CCC.CCGC.GGCTTTGTTCCTTTCTTCTTCCCGCGCTGGTCCGCGTGACCATCGCCCTGCAATCCGACCTTGGccccgcc                 >  1:475234/1‑96 (MQ=255)
                                                                                      cgcgCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTCGGCCAGGCCt                <  2:2313264/101‑1 (MQ=255)
                                                                                       gcgcTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTCGGCCAGGCCTc               <  1:1742179/101‑1 (MQ=255)
                                                                                       gcgcTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTCGGCCAGGCCTc               <  2:4891802/101‑1 (MQ=255)
                                                                                        cgcTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGGTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTCGGCCAGGCCTCg              >  2:4203664/1‑101 (MQ=255)
                                                                                           tGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCCCCCTGGCACTGCAGCCCGACTTCGGCCAGGCCTCGCTg           <  1:1435842/101‑1 (MQ=255)
                                                                                              aaGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTCGGCCAGGCCTCGCTGATc        <  1:4517024/101‑1 (MQ=255)
                                                                                                 tGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTCGGCCAGGCCTCGCTGATCctc     <  1:3273258/101‑1 (MQ=255)
                                                                                                  ggC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATGCTCGGCGCGCTGATCGTCGTCCCCCTGGCACTGCGGCCCGAGTTCGGCCAGGCCTCGCTGATCctct    <  2:5017961/101‑1 (MQ=255)
                                                                                                  ggC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTCGGCCAGGCCTCGCTGATCctct    >  1:1524802/1‑101 (MQ=255)
                                                                                                  ggC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTCGGCCAGGCCTCGCTGATCctct    >  2:779141/1‑101 (MQ=255)
                                                                                                    c.GGGC.CCG.CCC.GGGC.GGCTTTATTCCCTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTCGGCCAGGCCTCGCTGATCCTCttt  <  1:181742/101‑1 (MQ=255)
                                                                                                     |                                                                                                        
TGGATCTCGCTCGGCTTCGCCTCGTTCCAGCCTTCGGAATTCCTCAAGCCCGGCTTCGTCATCGTCTCGGCCTGGTTCATGGCCGCCGCGCTGGAAGTGGC.GGGC.CCG.CCC.GGGC.GGCTTTATTCCTTCATCCTCACCGCGCTGATCGTCGTCACCCTGGCACTGCAGCCCGACTTCGGCCAGGCCTCGCTGATCCTCTTT  >  NC_014034/901315‑901515

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG