Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,970,1411.→G98.7% 2.7 ‑0.5 ‑2.3303intergenic (+172/+161)tsf/RCAP_rcc01823translation elongation factor Ts/LuxR family autoinducer‑binding transcriptional regulator
Rejected: Prediction has significantly lower quality scores supporting polymorphism compared to reference.
Reads supporting (aligned to +/- strand):  new base (157/142):  ref base (3/1):  total (160/143)
Fisher's exact test strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.21e-03

Only 100 of 334 total aligned reads displayed.

CGCCCTGAACCGTCAGGGCGCGCTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.C.GGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAGGCATTTCCCATGGAT  >  NC_014034/1970041‑1970237
                                                                                                      |                                                                                                
 gcCCTGAACCGTCAGGGCGCGCTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.C.g                                                                                                 >  2:1958578/1‑101 (MQ=255)
 gcCCTGAACCGTCAGGGCGCGCTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.C.g                                                                                                 >  1:2802922/1‑101 (MQ=255)
  cccTGAACCGTCAGGGCGCGCTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCCCTGGGCCGTACCGGAAAAC.C.gg                                                                                                >  1:2439670/1‑101 (MQ=255)
    cTGAACCGTCAGGGCGCGCTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.C.gggt                                                                                              <  2:3250890/101‑3 (MQ=255)
      gAACCGTCAGGGCGCGCTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAANCNNNCTGGGCCGTNCCGGAAAAC.A.gggtgg                                                                                            >  1:691619/1‑97 (MQ=255)
      gAACCGTCAGGGCGCGCTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.C.gggtgg                                                                                            >  1:443520/1‑97 (MQ=255)
      gAACCGTCAGGGCGCGCTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.C.gggtgg                                                                                            >  1:3144323/1‑97 (MQ=255)
       aaCCGTCAGGGCGCGCTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.C.gggtggg                                                                                           >  2:644616/1‑96 (MQ=255)
           gTCAGGGCGCGCTTTCCTGCATATCTTGTGCATGCAATTGAAACGGCCCAGACGGGTGACTATAAACCAGCTGGGCCGTACCGGAAAAC.CAGGAGGGAtct                                                                                        >  2:1813715/1‑101 (MQ=255)
           gTCAGGGCGCGCTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGAtct                                                                                        >  2:1111950/1‑101 (MQ=255)
             cAGGGCGCGCTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCg                                                                                      <  1:486381/101‑1 (MQ=255)
              aGGGCGCGCTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGc                                                                                     >  1:4683659/1‑101 (MQ=255)
                 gcgcgcTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCgg                                                                                  <  2:2022234/101‑1 (MQ=255)
                   gcgcTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGAt                                                                                >  2:4555576/1‑101 (MQ=255)
                    cgttttCCTGCATATCTTTTGCATGAAATTGAAACGGCCCAGAGGGGTATCTATAAGCCCGCTGGGCCCTACCGGAAAAC.CGGGTGGGATCTCGCCGGATc                                                                               <  1:1836587/98‑1 (MQ=255)
                    cgcTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGCGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATc                                                                               >  1:1479504/1‑101 (MQ=255)
                     gcTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCa                                                                              <  1:196165/101‑1 (MQ=255)
                     gcTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCa                                                                              >  1:2502521/1‑101 (MQ=255)
                      cTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCaa                                                                             <  1:1178511/101‑1 (MQ=255)
                      cTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCaa                                                                             >  2:860622/1‑101 (MQ=255)
                        ttCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATc                                                                           >  2:1197772/1‑101 (MQ=255)
                         tCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCt                                                                          >  2:4798093/1‑101 (MQ=255)
                         tCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCt                                                                          >  2:4081120/1‑101 (MQ=255)
                         tCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCt                                                                          >  1:2281869/1‑101 (MQ=255)
                          ccTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCtt                                                                         <  2:3016907/101‑1 (MQ=255)
                            tGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCTGATCAATCTTCg                                                                       <  1:2739036/101‑1 (MQ=255)
                              cATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGAc                                                                     >  1:980234/1‑101 (MQ=255)
                              cATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGAc                                                                     <  1:4798416/101‑1 (MQ=255)
                               atatCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACt                                                                    >  2:3399261/1‑101 (MQ=255)
                               atatCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACt                                                                    >  2:3536357/1‑101 (MQ=255)
                                tatCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTg                                                                   <  1:1374642/101‑1 (MQ=255)
                                tatCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTg                                                                   >  2:1667264/1‑101 (MQ=255)
                                 atCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGc                                                                  >  1:756345/1‑101 (MQ=255)
                                 atCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGc                                                                  >  1:3595760/1‑101 (MQ=255)
                                   cTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCtt                                                                >  1:3264689/1‑101 (MQ=255)
                                     tgtgCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTcc                                                              <  1:2011994/101‑1 (MQ=255)
                                     tgtgCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTcc                                                              >  1:300148/1‑101 (MQ=255)
                                        gCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTg                                                           >  2:4829813/1‑101 (MQ=255)
                                        gCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTg                                                           >  1:1585299/1‑101 (MQ=255)
                                         cATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGt                                                          <  2:3489248/101‑1 (MQ=255)
                                          aTGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGtt                                                         <  1:2317637/101‑1 (MQ=255)
                                          aTGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGtt                                                         >  2:1343773/1‑101 (MQ=255)
                                          aTGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGtt                                                         >  1:2175641/1‑101 (MQ=255)
                                          aTGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGtt                                                         <  2:5016457/101‑1 (MQ=255)
                                           tGAAATTGAAACGGCCCAGACGTGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTc                                                        <  2:2754100/101‑1 (MQ=255)
                                           tGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTc                                                        >  2:2827461/1‑101 (MQ=255)
                                            gAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTcc                                                       >  1:909812/1‑101 (MQ=255)
                                              aaTTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTg                                                     >  1:3669121/1‑101 (MQ=255)
                                              aaTTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTg                                                     >  2:4422772/1‑101 (MQ=255)
                                                 tGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGcc                                                  >  1:1014411/1‑101 (MQ=255)
                                                  gAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCg                                                 >  2:937965/1‑101 (MQ=255)
                                                  gAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCg                                                 >  1:85583/1‑101 (MQ=255)
                                                   aaaCGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGa                                                >  1:4740135/1‑101 (MQ=255)
                                                    aaCGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGaa                                               <  2:3134251/101‑1 (MQ=255)
                                                    aaCGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGaa                                               >  1:1107652/1‑101 (MQ=255)
                                                         cccAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGccac                                          <  1:3979840/101‑1 (MQ=255)
                                                          ccAGACGGGTGACTATAACCCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGccacc                                         <  2:2925645/101‑1 (MQ=255)
                                                          ccAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGccacc                                         <  2:443455/101‑1 (MQ=255)
                                                          ccAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGccacc                                         >  1:4084717/1‑101 (MQ=255)
                                                           cAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGccacca                                        <  1:289448/101‑1 (MQ=255)
                                                           cAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGccacca                                        >  2:3634760/1‑101 (MQ=255)
                                                            aGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCcaccac                                       >  1:1231772/1‑101 (MQ=255)
                                                             gACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACt                                      >  1:1726008/1‑101 (MQ=255)
                                                               ctggTGACTATAAACCCGCTGGGCCGTACCGGAAAACTG.GGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCa                                    <  1:3513009/99‑1 (MQ=255)
                                                                 ggTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACg                                  <  1:3894513/101‑1 (MQ=255)
                                                                   gtaccaaaaaCCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGa                                <  1:271946/94‑1 (MQ=255)
                                                                    gACTATAAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGaa                               >  1:2584312/1‑101 (MQ=255)
                                                                       tataAACCCGCTGGGCAGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAacac                            >  2:1452627/1‑101 (MQ=255)
                                                                        ataAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACg                           >  2:1489918/1‑101 (MQ=255)
                                                                        ataAACCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACg                           <  1:3392407/101‑1 (MQ=255)
                                                                          aaaCCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACgtg                         >  1:4472946/1‑101 (MQ=255)
                                                                          aaaCCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACgtg                         >  1:4982922/1‑101 (MQ=255)
                                                                           aaCCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACgtgt                        <  2:768876/101‑1 (MQ=255)
                                                                           aaCCCGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACgtgt                        <  2:2105357/101‑1 (MQ=255)
                                                                             cccGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTAt                      <  2:912456/101‑1 (MQ=255)
                                                                               cGCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCt                    <  2:4464361/101‑1 (MQ=255)
                                                                                gCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTa                   <  2:1621742/101‑1 (MQ=255)
                                                                                gCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTa                   >  1:429853/1‑101 (MQ=255)
                                                                                gCTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTa                   <  1:804571/101‑1 (MQ=255)
                                                                                 cTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTaa                  <  2:2179514/101‑1 (MQ=255)
                                                                                 cTGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTaa                  <  2:26806/101‑1 (MQ=255)
                                                                                  tGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAg                 <  2:2281705/101‑1 (MQ=255)
                                                                                  tGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAg                 >  2:4032342/1‑101 (MQ=255)
                                                                                  tGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAg                 >  2:2471325/1‑101 (MQ=255)
                                                                                  tGGGCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAg                 >  2:1875813/1‑101 (MQ=255)
                                                                                   gggCCGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAgg                >  2:3285318/1‑101 (MQ=255)
                                                                                      ccGTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAGGCAt             <  2:3784421/101‑1 (MQ=255)
                                                                                        gTACCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAGGCAttt           >  2:2547704/1‑101 (MQ=255)
                                                                                          aCCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAGGCATTTcc         <  2:4240481/101‑1 (MQ=255)
                                                                                          aCCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAGGCATTTcc         <  1:3058442/101‑1 (MQ=255)
                                                                                          aCCGGAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGACGAAGCCACCACTCACGGAACACGTGTATCTAAGGCATTTcc         <  1:3801478/101‑1 (MQ=255)
                                                                                             ggAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAGGCATTTccatg      <  2:4751983/101‑4 (MQ=255)
                                                                                             ggAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAGGCATTTccatg      <  2:4982592/101‑4 (MQ=255)
                                                                                             ggAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAGGCATTTccatg      >  1:4225414/1‑98 (MQ=255)
                                                                                             ggAAAAC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCGAAGGCATTTccatg      >  1:5022412/1‑98 (MQ=255)
                                                                                                aaaC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAGGCATTT.CCATGGAt  >  1:1749402/1‑101 (MQ=255)
                                                                                                aaaC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAGGCATTT.CCATGGAt  >  2:3862612/1‑101 (MQ=255)
                                                                                                aaaC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAGGCATTT.CCATGGAt  >  2:2391979/1‑101 (MQ=255)
                                                                                                aaaC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAGGCATTT.CCATGGAt  >  2:1749753/1‑101 (MQ=255)
                                                                                                aaaC.CGGGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAGGCATTT.CCATGGAt  >  1:4564002/1‑101 (MQ=255)
                                                                                                      |                                                                                                
CGCCCTGAACCGTCAGGGCGCGCTTTCCTGCATATCTTGTGCATGAAATTGAAACGGCCCAGACGGGTGACTATAAACCCGCTGGGCCGTACCGGAAAAC.C.GGTGGGATCTCGCCGGATCAATCTTCGACTGCTTCCCTGTTCCTGGCCGAAGCCACCACTCACGGAACACGTGTATCTAAGGCATTTCCCATGGAT  >  NC_014034/1970041‑1970237

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG