Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,476 T→C 18.8% intergenic (+288/‑643) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4760T→C18.8% 123.7 ‑1.2 0.0281intergenic (+288/‑643)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (21/32):  ref base (110/118):  total (131/150)
Fisher's exact test strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

CGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCC  >  NC_014034/1395376‑1395576
                                                                                                    |                                                                                                     
 gcgGGGGCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTg                                                                                                      <  1:2049402/101‑1 (MQ=255)
 gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTg                                                                                                      <  2:3032144/101‑1 (MQ=255)
     gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCgg                                                                                                  >  1:3857868/1‑101 (MQ=255)
      ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                                                                                 <  2:3462169/101‑1 (MQ=255)
        cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTTTTCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                                                                               >  2:1676202/1‑101 (MQ=255)
         gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                                                                                              >  2:2527687/1‑101 (MQ=255)
          tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                                                                             <  2:537328/101‑1 (MQ=255)
           ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa                                                                                            >  2:4778598/1‑101 (MQ=255)
            gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                                                                           <  1:2928791/101‑1 (MQ=255)
            gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                                                                           <  2:3721125/101‑1 (MQ=255)
             aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGAGCGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTTGACAgg                                                                                          <  2:866079/101‑1 (MQ=255)
                gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                                                                       >  1:5048942/1‑101 (MQ=255)
                gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                                                                       <  1:2880644/101‑1 (MQ=255)
                   aCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                                                                                    >  2:1690843/1‑101 (MQ=255)
                    cTGCACCCGCTGGCCCCGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTaa                                                                                   >  1:149226/1‑101 (MQ=255)
                     tGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc                                                                                  <  2:4243897/101‑1 (MQ=255)
                      gcccccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.g                                                                                <  2:4082931/98‑1 (MQ=255)
                      gcccccGCGGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.g                                                                                <  2:639599/98‑1 (MQ=255)
                      gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTaagg                                                                                 >  1:679051/1‑99 (MQ=255)
                         cccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.gtgt                                                                             <  1:2263058/101‑1 (MQ=255)
                          ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTc                                                                            >  1:1097574/1‑101 (MQ=255)
                               ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTcc                                                                       >  1:3254709/1‑101 (MQ=255)
                               ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGATGGTAAC.GTGTCGCTcc                                                                       >  2:719928/1‑101 (MQ=255)
                                 cccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTcccc                                                                     <  2:444843/101‑1 (MQ=255)
                                 cccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTcccc                                                                     >  2:4610795/1‑101 (MQ=255)
                                 cccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTcccc                                                                     >  2:1893571/1‑101 (MQ=255)
                                 cccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTcccc                                                                     >  2:3230512/1‑101 (MQ=255)
                                 cccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTcccc                                                                     >  1:1773965/1‑101 (MQ=255)
                                  ccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTccccc                                                                    >  1:2344285/1‑101 (MQ=255)
                                   cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTcccccc                                                                   <  1:3042657/101‑1 (MQ=255)
                                   cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTcccccc                                                                   <  2:3854401/101‑1 (MQ=255)
                                     gCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCcgc                                                                 >  1:2125576/1‑101 (MQ=255)
                                       cggtgatcggtgatCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCgg                                                               >  2:1387923/1‑101 (MQ=255)
                                         gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCgggg                                                             >  1:4410603/1‑101 (MQ=255)
                                           gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGGTCCCCCCGCgggggg                                                           >  2:1533261/1‑100 (MQ=255)
                                           gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGc                                                           >  2:1018746/1‑101 (MQ=255)
                                           gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGc                                                           <  2:3679680/101‑1 (MQ=255)
                                            atcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCg                                                          >  2:3654032/1‑101 (MQ=255)
                                              cggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTg                                                        >  1:581195/1‑101 (MQ=255)
                                               ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTgg                                                       >  1:2653659/1‑101 (MQ=255)
                                               ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTgg                                                       <  2:3176939/101‑1 (MQ=255)
                                                gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGa                                                      <  1:3499439/101‑1 (MQ=255)
                                                 tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGtcgca                                                     >  1:1393888/1‑97 (MQ=255)
                                                 tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                     <  1:1902821/101‑1 (MQ=255)
                                                 ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACG.GAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                    <  2:4939415/93‑1 (MQ=0)
                                                 ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACG.GAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                    <  1:4009993/93‑1 (MQ=0)
                                                  gatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                    >  1:3378767/1‑101 (MQ=255)
                                                  gatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                    <  2:2654035/101‑1 (MQ=255)
                                                   ggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACG.GAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                  >  1:3134655/7‑101 (MQ=2)
                                                   atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  1:4755427/101‑1 (MQ=255)
                                                   accgggggTCGCCCCCCCCGCGGGGGGGGGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCCCCCCCCGCGGGGGCGTGGATCg                                                   <  2:1257018/95‑1 (MQ=38)
                                                    tcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                  <  1:747863/101‑1 (MQ=255)
                                                      cgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACG.GAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                               <  1:1693482/98‑1 (MQ=2)
                                                       gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACG.GAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                              >  1:493847/3‑101 (MQ=2)
                                                       gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACG.GAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                              <  1:2754232/99‑1 (MQ=2)
                                                       gtggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACG.GAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                              <  2:3582553/99‑1 (MQ=2)
                                                       gCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                               <  2:4873420/101‑1 (MQ=255)
                                                        cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                              <  2:1983638/101‑1 (MQ=255)
                                                        cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                              >  1:4576618/1‑101 (MQ=255)
                                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACG.GAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                            >  2:2210255/1‑99 (MQ=2)
                                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                             >  1:2289729/1‑101 (MQ=255)
                                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                             <  2:1483717/101‑1 (MQ=255)
                                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                             <  1:1218691/101‑1 (MQ=255)
                                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAAt                                             >  2:2088410/1‑101 (MQ=255)
                                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtc                                            <  1:164082/101‑1 (MQ=255)
                                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtct                                           <  1:4973501/101‑1 (MQ=255)
                                                            cccaccccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                          <  2:4154969/97‑1 (MQ=255)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACG.GAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgcttt                                         >  1:392142/1‑99 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACG.GAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgcttt                                         >  1:2560976/1‑99 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                          >  2:4387371/1‑101 (MQ=255)
                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACG.GAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                       <  2:4340145/101‑5 (MQ=2)
                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACG.GAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                       <  2:308916/101‑5 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACG.GAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaa                                      >  1:3411170/1‑96 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACG.GAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaa                                      >  1:2200852/1‑96 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                                       <  1:360359/101‑1 (MQ=255)
                                                                  ccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGcc                                    <  1:4796121/101‑1 (MQ=255)
                                                                   cccGCGGGGGCGTGGATCGAAACTACGCCGGGGCTCTGGTC.AC.GGTTTGTGACGGTGTCGCACCCCCCGCGGGGGCGTGGACCGAAACAgctttcaatggg                                  <  1:2471888/101‑10 (MQ=2)
                                                                    ccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGt                                  <  2:67312/101‑1 (MQ=255)
                                                                      gcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGGGCCGTCg                                >  2:419310/1‑101 (MQ=255)
                                                                        gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCt                              <  2:951424/101‑1 (MQ=255)
                                                                        gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCt                              >  2:1074800/1‑101 (MQ=255)
                                                                         ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTc                             <  2:1107056/101‑1 (MQ=255)
                                                                            gCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAATATCTCGGTGCCGTCGCTCGTc                          <  1:3727499/101‑1 (MQ=255)
                                                                             cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTcc                         <  2:247596/101‑1 (MQ=255)
                                                                               tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAg                       <  2:3637813/101‑1 (MQ=255)
                                                                                    cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGGCGTCGCTCGTCCAGCCGGc                  >  2:1765393/1‑101 (MQ=255)
                                                                                    cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGc                  >  2:1510905/1‑101 (MQ=255)
                                                                                     gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGcc                 >  2:2132770/1‑101 (MQ=255)
                                                                                      aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCt                <  2:1571890/101‑1 (MQ=255)
                                                                                          aTCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc            <  1:4107146/101‑1 (MQ=255)
                                                                                          aTCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGCCCAGCCGGCCTGCtc            >  1:1076904/1‑101 (MQ=255)
                                                                                            cgccgcTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgt          <  1:875652/101‑1 (MQ=255)
                                                                                            cgccgcTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgt          >  2:336874/1‑101 (MQ=255)
                                                                                              ccgcTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtcg        <  2:2879717/101‑1 (MQ=255)
                                                                                               cgcTTTGGCGGTCCACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATTGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGc       <  1:1554760/101‑1 (MQ=255)
                                                                                                gcTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCt      >  1:3657427/1‑101 (MQ=255)
                                                                                                  tttGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcc    >  2:3948998/1‑101 (MQ=255)
                                                                                                   ttGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTccc   >  2:2302729/1‑101 (MQ=255)
                                                                                                   ttGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTccc   >  2:2384027/1‑101 (MQ=255)
                                                                                                    tGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcccc  >  2:2611762/1‑101 (MQ=255)
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CGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC.GTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCC  >  NC_014034/1395376‑1395576

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG