Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,534 C→T 12.1% intergenic (+346/‑585) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5340C→T12.1% 93.0 ‑1.4 0.0354intergenic (+346/‑585)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/26):  ref base (132/152):  total (157/197)
Fisher's exact test strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 405 total aligned reads displayed.

GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGT  >  NC_014034/1395434‑1395634
                                                                                                    |                                                                                                       
gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtc                                                                                                         <  2:3659068/101‑1 (MQ=255)
 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTAGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctt                                                                                                       <  1:3729735/101‑2 (MQ=2)
  cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgcttt                                                                                                      >  1:392142/1‑99 (MQ=2)
  cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgcttt                                                                                                      <  1:4939491/101‑3 (MQ=2)
  cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGCCGCGCCCCCCGCGGGGGCGTGGATCGAAACAgcttt                                                                                                      >  1:4659003/1‑99 (MQ=0)
   gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                                                                                      <  1:2899007/101‑1 (MQ=255)
   ccTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                                                                                     <  2:3503762/100‑4 (MQ=2)
     tCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaa                                                                                                   >  1:3411170/1‑96 (MQ=2)
      ccccccGCGGGGGCGTGGATCGAAACATCGCCGCTTCGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTg                                                                                                   >  2:4564505/1‑101 (MQ=255)
        ccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGcc                                                                                                 <  2:4350643/101‑1 (MQ=255)
          ccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGt                                                                                               <  2:67312/101‑1 (MQ=255)
           cgcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTc                                                                                              <  2:1569565/101‑1 (MQ=255)
              gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCt                                                                                           >  2:1074800/1‑101 (MQ=255)
              gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCt                                                                                           >  2:2515044/1‑101 (MQ=255)
               ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTc                                                                                          <  2:1107056/101‑1 (MQ=255)
                   cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                     <  2:247596/101‑1 (MQ=255)
                    gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                    >  2:5029150/1‑101 (MQ=255)
                     tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                   >  2:4909123/1‑101 (MQ=255)
                      ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGc                                                                                  >  2:3615592/1‑101 (MQ=255)
                      ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGc                                                                                  >  2:2635514/1‑101 (MQ=255)
                      ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGc                                                                                  >  2:4170855/1‑101 (MQ=255)
                      ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCCCG.Tccctc                                                                                  >  2:4713014/1‑98 (MQ=255)
                       gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGc.c                                                                                >  2:3794918/1‑101 (MQ=255)
                        aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.Cg                                                                               <  1:3654304/101‑1 (MQ=255)
                          cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGGCGTCGCTCG.TCCAGC.CGG.c                                                                            >  2:1765393/1‑101 (MQ=255)
                          cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.c                                                                            >  2:1510905/1‑101 (MQ=255)
                           gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.cc                                                                           >  2:2132770/1‑101 (MQ=255)
                           gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.cc                                                                           >  2:3227081/1‑101 (MQ=255)
                                aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCtc                                                                      <  1:4107146/101‑1 (MQ=255)
                                    ccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCtcgtcg                                                                  <  2:2879717/101‑1 (MQ=255)
                                      gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCt                                                                <  1:1226776/101‑1 (MQ=255)
                                      gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.ACTGCTCTTCGcc                                                                >  2:4214950/1‑100 (MQ=255)
                                       cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTc                                                               >  1:2256477/1‑101 (MQ=255)
                                         ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTccc                                                             >  2:2384027/1‑101 (MQ=255)
                                          tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTcccc                                                            <  2:2082466/101‑1 (MQ=255)
                                          tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTcccc                                                            >  1:1677328/1‑101 (MQ=255)
                                             gtgtggataGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCcg                                                         <  2:401108/93‑1 (MQ=255)
                                                 cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCggg                                                     <  2:34000/101‑1 (MQ=255)
                                                   aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCggggg                                                   >  1:3094561/1‑101 (MQ=255)
                                                        gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATG.GCTGGC.CGC.CCCGCCCCTCGCTCCCCCCGCGGGGGCGTgg                                              >  2:2014018/11‑101 (MQ=2)
                                                        cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTgg                                              >  1:4234010/1‑101 (MQ=255)
                                                        cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTgg                                              <  2:3051784/101‑1 (MQ=255)
                                                        cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTgg                                              >  1:107815/1‑101 (MQ=255)
                                                        agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTgg                                              <  1:82140/91‑1 (MQ=0)
                                                         gcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                             <  1:477855/92‑1 (MQ=0)
                                                         ccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGa                                             <  1:62857/92‑1 (MQ=2)
                                                          ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                            >  1:4833911/9‑101 (MQ=2)
                                                          ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                            >  1:2300646/1‑101 (MQ=255)
                                                          ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  1:4017875/101‑1 (MQ=255)
                                                          ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  2:3092213/101‑1 (MQ=255)
                                                          ggTAACGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  1:1795421/101‑1 (MQ=255)
                                                           gccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:2483963/94‑1 (MQ=2)
                                                           gccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:4574011/94‑1 (MQ=2)
                                                           gTAACGTTTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                           >  2:2247891/1‑101 (MQ=255)
                                                           gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                           >  1:2212397/1‑101 (MQ=255)
                                                            tAACGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  2:5693/101‑1 (MQ=255)
                                                             cggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         <  1:490431/96‑1 (MQ=2)
                                                              gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  1:3695411/97‑1 (MQ=2)
                                                              gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                        >  1:4421915/5‑101 (MQ=2)
                                                              aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        >  1:433048/1‑101 (MQ=255)
                                                               cgcgtCGCCCCCCCCGGGGGGGCGTGGATCGAAACACCCCGAACCTGTCG.GCG.GCGAACACCG.ATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  1:2752067/98‑1 (MQ=2)
                                                                tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                      <  2:2170493/99‑1 (MQ=2)
                                                                tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                      <  2:2790760/99‑1 (MQ=2)
                                                                cggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                      <  2:3173527/99‑1 (MQ=2)
                                                                 tgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                     <  2:1931878/101‑1 (MQ=255)
                                                                  gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacca                                    >  1:3591251/1‑101 (MQ=255)
                                                                  gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactg                                    <  2:392077/101‑3 (MQ=2)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                   >  1:55929/1‑98 (MQ=2)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                   <  1:2016563/101‑4 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                  <  1:3949290/101‑1 (MQ=255)
                                                                     gccccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgt                                 <  1:4462868/98‑6 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgc                                 >  1:910194/1‑96 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgc                                 >  1:4978266/1‑96 (MQ=2)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccccg                                >  2:908102/1‑99 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                               <  1:216669/101‑8 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                               <  1:2438192/101‑8 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                               >  1:3270682/1‑94 (MQ=2)
                                                                       cccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                               <  2:4506471/100‑8 (MQ=2)
                                                                        ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                              <  1:1542028/101‑1 (MQ=255)
                                                                        ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcg                              <  2:1711472/101‑9 (MQ=2)
                                                                        ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACAGGATGTCGCTCCCCCCGCGGGGGCGGGGATCGAAactatgctgg                              >  1:4546155/1‑93 (MQ=2)
                                                                         cccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCg                             <  1:2983954/101‑1 (MQ=255)
                                                                             cgcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                         <  1:336927/101‑1 (MQ=255)
                                                                              gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                        <  1:3502072/101‑1 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTgg                       >  2:38588/1‑100 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                       >  2:2685573/1‑101 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                       >  1:1301224/1‑101 (MQ=255)
                                                                                   ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                   <  2:149210/101‑1 (MQ=255)
                                                                                   ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                   >  1:684189/1‑101 (MQ=255)
                                                                                     cGTGGATCGAACCATCTCGGGGCCGGCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                 <  2:4443372/101‑1 (MQ=255)
                                                                                     cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                 >  1:1926979/1‑101 (MQ=255)
                                                                                     cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                 <  1:1018856/101‑1 (MQ=255)
                                                                                     cGTGGATCAAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                 <  1:1507009/101‑1 (MQ=255)
                                                                                       tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg               <  1:2132918/101‑1 (MQ=255)
                                                                                            cGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgggg          <  1:3844658/101‑1 (MQ=255)
                                                                                            cGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgggg          >  1:1613284/1‑101 (MQ=255)
                                                                                             gAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc         <  1:1718154/101‑1 (MQ=255)
                                                                                               aaCATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa       <  2:2982933/101‑1 (MQ=255)
                                                                                                    ctcGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGt  >  2:1621199/1‑101 (MQ=255)
                                                                                                    ctcGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGt  <  2:1301103/101‑1 (MQ=255)
                                                                                                    |                                                                                                       
GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGT  >  NC_014034/1395434‑1395634

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG