Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,669 G→C 8.5% intergenic (+481/‑450) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6690G→C 8.5% 41.3 ‑1.3 ‑0.6249intergenic (+481/‑450)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/12):  ref base (126/100):  total (136/113)
Fisher's exact test strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.71e-01

Only 100 of 269 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGC  >  NC_014034/1395569‑1395768
                                                                                                    |                                                                                                      
cgCCCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTg                                                                                                        <  1:3795203/101‑1 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTTACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGc                                                                                                       >  1:3144265/1‑101 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGc                                                                                                       >  1:2697026/1‑101 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGc                                                                                                       >  2:3610793/1‑101 (MQ=255)
  cTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCa                                                                                                      <  1:2302893/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGt                                                                                                >  1:4588781/1‑101 (MQ=255)
          gcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcg                                                                                              >  2:1744567/1‑101 (MQ=255)
           cgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg                                                                                             <  1:2212940/101‑1 (MQ=255)
            gggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggc                                                                                            >  2:3481978/1‑101 (MQ=255)
             ggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggcg                                                                                           >  2:59174/1‑101 (MQ=255)
              gggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggcgg                                                                                          >  1:5004877/1‑101 (MQ=255)
               ggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCgg..g                                                                                       >  1:2617682/1‑101 (MQ=255)
                gCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..Gc                                                                                      <  2:222938/101‑1 (MQ=255)
                 cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCa                                                                                     >  2:299656/1‑101 (MQ=255)
                   tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGt                                                                                   >  1:970804/1‑101 (MQ=255)
                   tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGt                                                                                   >  1:1488893/1‑101 (MQ=255)
                   tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAgg                                                                                   <  2:2370631/101‑2 (MQ=255)
                    ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGtt                                                                                  <  2:1509675/101‑1 (MQ=255)
                    ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGtt                                                                                  <  1:1615724/101‑1 (MQ=255)
                    ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGtt                                                                                  >  1:4216302/1‑101 (MQ=255)
                    ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGtt                                                                                  >  1:4010234/1‑101 (MQ=255)
                      aTCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCg                                                                                <  1:1499738/101‑1 (MQ=255)
                         taaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.Gc                                                                            <  2:2145235/100‑1 (MQ=255)
                         gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.Gc                                                                            <  1:522476/101‑1 (MQ=255)
                          aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.Gcc                                                                           <  2:725296/101‑1 (MQ=255)
                            accaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGGGG..GCAGTTCGA.GCCGc                                                                         >  1:1953396/1‑101 (MQ=255)
                            accaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGc                                                                         >  1:4538627/1‑101 (MQ=255)
                            accaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGc                                                                         >  2:4399477/1‑101 (MQ=255)
                               cccTTGCGGATTGTCACTTTCGCGGGGCGAAGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGt                                                                      <  1:2087942/100‑1 (MQ=255)
                                 cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCg                                                                    >  2:3218459/1‑101 (MQ=255)
                                 cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGCTCGA.GCCGCAGTCg                                                                    >  1:4360436/1‑101 (MQ=255)
                                    gCGGATTGTCTCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTc                                                                 <  1:2165062/101‑1 (MQ=255)
                                    gCGGATTGCGACTTTGGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAGCATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTc                                                                 >  2:334729/1‑101 (MQ=38)
                                     cGGATTGTCACTTTCGCGGGGCGAGGCGTCGCACCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGCCGG..GCAGTTCGA.GCCGCAGTCGCTcc                                                                <  2:4747033/101‑1 (MQ=255)
                                      ggATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTccc                                                               <  1:119712/101‑1 (MQ=255)
                                      ggATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTccc                                                               >  1:4403890/1‑101 (MQ=255)
                                         ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTcccccc                                                            >  1:2653001/1‑101 (MQ=255)
                                         ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTcccccc                                                            >  2:4413192/1‑101 (MQ=255)
                                         ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTcccccc                                                            >  2:837391/1‑101 (MQ=255)
                                         ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGGTCGA.GCCGCAGTCGCTcccccc                                                            >  2:4830026/1‑101 (MQ=255)
                                          tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                           >  2:1144047/1‑101 (MQ=255)
                                          tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                           >  2:2108197/1‑101 (MQ=255)
                                          tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                           <  2:1076791/101‑1 (MQ=255)
                                          tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                           <  2:2587669/101‑1 (MQ=255)
                                           gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                          >  1:325436/1‑101 (MQ=255)
                                               cTTTCGCGGGGCGAGGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgggg                                                      <  2:4204907/101‑1 (MQ=255)
                                               cTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgggg                                                      >  2:3282321/1‑101 (MQ=255)
                                                 ttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGc                                                    >  1:1020281/1‑101 (MQ=255)
                                                    tggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGt                                                  <  1:31130/91‑1 (MQ=2)
                                                     gggggCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTgg                                                <  1:1100674/100‑1 (MQ=255)
                                                     cgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTgg                                                >  2:4949503/1‑101 (MQ=255)
                                                      ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGa                                               <  1:3762838/101‑1 (MQ=255)
                                                        ggggAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:837493/98‑1 (MQ=255)
                                                        ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCCCGGGGGGGTGGATc                                             >  2:3779271/1‑101 (MQ=255)
                                                        gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                              >  1:1698323/7‑101 (MQ=2)
                                                         gCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                            <  1:720017/101‑1 (MQ=255)
                                                         ccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:2234570/96‑1 (MQ=2)
                                                          cGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                           <  1:3419938/101‑1 (MQ=255)
                                                            ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  1:2523865/99‑1 (MQ=2)
                                                            ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  2:2016318/99‑1 (MQ=2)
                                                             aGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                        >  2:393591/1‑101 (MQ=255)
                                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                      >  2:4326350/1‑101 (MQ=255)
                                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGC.GAACACCGA.TACCGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                       >  1:4298074/1‑100 (MQ=2)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                     >  2:2690386/1‑101 (MQ=255)
                                                                 tcccTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                    <  1:3689995/98‑1 (MQ=255)
                                                                     cccccccGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGGGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                <  1:1760071/100‑1 (MQ=255)
                                                                     cccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                <  2:216899/100‑1 (MQ=255)
                                                                     cccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                <  1:2108345/100‑1 (MQ=255)
                                                                      ccccccGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAGCGGCAGGGTTTCCA.GCGGC.GTCCCTCCCCCCCCGGGGGCGGGGATCGAAAcccgccccg                                >  1:2049403/1‑93 (MQ=0)
                                                                        ccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATc                             <  2:1935384/101‑1 (MQ=255)
                                                                            gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                         <  1:3567444/101‑1 (MQ=255)
                                                                            gcaggggCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                         >  1:2892064/4‑101 (MQ=255)
                                                                              gggggGCTGGACCGAAATATCTGCGTGTAGTCGGGGG..GCTGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                       <  2:3835088/101‑1 (MQ=25)
                                                                                 ggCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaaa                    >  1:728828/1‑101 (MQ=255)
                                                                                 ggCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaaa                    >  2:521171/1‑101 (MQ=255)
                                                                                    gTGGATCGAACCATCTGCATGTAGTGGGCGG..GCGGTTGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                 <  2:4046148/101‑1 (MQ=37)
                                                                                     tGGCTCGAAACATCTGCTTGTAGGCGGCGG..GGAGTGGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGAGCTGGCGCAAACGGa                <  1:334782/101‑1 (MQ=25)
                                                                                     tGGATCGAAACATCTGCATGTAGTGGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                <  2:1860631/101‑1 (MQ=255)
                                                                                     tGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                >  1:1350671/1‑101 (MQ=255)
                                                                                     tGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                >  1:4838796/1‑101 (MQ=255)
                                                                                      ggATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa               <  1:4171172/101‑1 (MQ=255)
                                                                                      ggATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa               >  2:170361/1‑101 (MQ=255)
                                                                                      ggATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa               >  2:2285565/1‑101 (MQ=255)
                                                                                       ggtCGAAGCATCTGGTGGTGGGCGGCGG..GCGGTTGGA.GCCGGAGTGGCGCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa              <  1:3611064/99‑1 (MQ=21)
                                                                                       gATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa              >  2:3665253/1‑101 (MQ=255)
                                                                                       gATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa              <  1:2981204/101‑1 (MQ=255)
                                                                                        aTCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg             <  2:3590980/101‑1 (MQ=255)
                                                                                        aTCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg             <  2:1310250/101‑1 (MQ=255)
                                                                                         tCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGt            >  1:2593528/1‑101 (MQ=255)
                                                                                          cGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc           >  2:2767760/1‑101 (MQ=255)
                                                                                          cGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc           >  2:4527218/1‑101 (MQ=255)
                                                                                          cGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGCATGGATCTGGCGCAAACGGAAAGTc           >  2:291157/1‑101 (MQ=255)
                                                                                            aaaCATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg         >  1:2734176/1‑101 (MQ=255)
                                                                                            aaaCATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg         <  1:89249/101‑1 (MQ=255)
                                                                                               cATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcg      >  2:4155464/1‑101 (MQ=255)
                                                                                               cATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcg      >  1:2817565/1‑101 (MQ=255)
                                                                                                aTCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt     <  2:1616498/101‑1 (MQ=255)
                                                                                                 tCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc    >  2:2678765/1‑101 (MQ=255)
                                                                                                  cTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg   >  1:2398007/1‑101 (MQ=255)
                                                                                                   tGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGc  >  2:2857642/1‑101 (MQ=255)
                                                                                                    |                                                                                                      
CGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGC  >  NC_014034/1395569‑1395768

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG