Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,693 G→A 8.5% intergenic (+505/‑426) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6930G→A 8.5% 33.6 ‑1.8 0.0234intergenic (+505/‑426)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/13):  ref base (110/103):  total (118/116)
Fisher's exact test strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

CGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395593‑1395793
                                                                                                         |                                                                                                    
 gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.Gc                                                                                                     <  1:522476/101‑1 (MQ=255)
 gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.Gc                                                                                                     >  2:2026657/1‑101 (MQ=255)
 gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.Gc                                                                                                     >  2:3124007/1‑101 (MQ=255)
   aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCg                                                                                                   <  1:2624974/101‑1 (MQ=255)
    accaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGGG..G..GCAGTTCGA.GCCGc                                                                                                  >  1:1953396/1‑101 (MQ=255)
    accaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGc                                                                                                  >  1:4538627/1‑101 (MQ=255)
    accaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGc                                                                                                  >  2:4399477/1‑101 (MQ=255)
      ccccTTGCGGATTTTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAg                                                                                                <  2:1677199/99‑1 (MQ=255)
       cccTTGCGGATTGTCACTTTCGCGGGGCGAAGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGt                                                                                               <  1:2087942/100‑1 (MQ=255)
         cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGCTCGA.GCCGCAGTCg                                                                                             >  1:4360436/1‑101 (MQ=255)
           tGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCt                                                                                           <  2:2908564/101‑1 (MQ=255)
            gCGGATTGTCTCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTc                                                                                          <  1:2165062/101‑1 (MQ=255)
            gCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTc                                                                                          >  2:4212090/1‑101 (MQ=255)
            gCGGATTGCGACTTTGGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAGCATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTc                                                                                          >  2:334729/1‑101 (MQ=38)
             cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTcc                                                                                         <  1:2733478/101‑1 (MQ=255)
               gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTcccc                                                                                       >  1:3359698/1‑101 (MQ=255)
               gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTcccc                                                                                       >  2:1759941/1‑101 (MQ=255)
                 ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTcccccc                                                                                     >  1:2653001/1‑101 (MQ=255)
                 ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTcccccc                                                                                     <  2:4331844/101‑1 (MQ=255)
                 ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTcccccc                                                                                     >  2:4413192/1‑101 (MQ=255)
                  tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                                                    <  2:1076791/101‑1 (MQ=255)
                   gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                                                   <  2:4026298/101‑1 (MQ=255)
                         ttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGc                                                                             >  1:1020281/1‑101 (MQ=255)
                             gggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  2:2146594/100‑1 (MQ=255)
                             gggggCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  1:1100674/100‑1 (MQ=255)
                              ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                        >  1:2585481/1‑101 (MQ=255)
                               gggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       >  1:4273876/1‑101 (MQ=255)
                                ggggAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  1:837493/98‑1 (MQ=255)
                                ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATTTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  2:3336160/101‑1 (MQ=255)
                                gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       >  1:891788/7‑101 (MQ=2)
                                gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       >  1:1698323/7‑101 (MQ=2)
                                 gCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     >  1:3793015/1‑101 (MQ=255)
                                 gCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     <  1:720017/101‑1 (MQ=255)
                                 ccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  2:1007073/96‑1 (MQ=2)
                                 ccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  1:3172332/96‑1 (MQ=2)
                                 ccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  2:2527541/96‑1 (MQ=2)
                                 ccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  1:2234570/96‑1 (MQ=2)
                                  cGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    <  1:3419938/101‑1 (MQ=255)
                                   gctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    >  1:4118330/4‑101 (MQ=2)
                                   gctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    >  1:2233440/4‑101 (MQ=2)
                                    ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   <  2:5020392/99‑1 (MQ=2)
                                    ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   <  1:2523865/99‑1 (MQ=2)
                                     tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGGTATGGCGTCGGTGTCA..GGTTTTCCA.GCGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  >  2:804286/2‑101 (MQ=2)
                                     gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.TACACCGGGGCTCTGGT..G..ACGGTTTGG.GGCGGAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcg                                                                >  2:3242960/4‑100 (MQ=2)
                                     aGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 <  1:2762736/101‑1 (MQ=255)
                                      gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 <  2:2927946/101‑1 (MQ=2)
                                      gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 >  2:2608704/1‑101 (MQ=2)
                                       cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GC.GAACACCGA.TACCGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                                >  1:4298074/1‑100 (MQ=2)
                                         tcccTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                                             <  1:3689995/98‑1 (MQ=255)
                                            cTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATg                                                          >  1:4599649/1‑101 (MQ=255)
                                            cTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATg                                                          <  2:3657155/101‑1 (MQ=255)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGGGGGGGTCGAAAcacccatg                                                          >  1:1714131/1‑94 (MQ=2)
                                             cccccccGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                         <  2:4906255/100‑1 (MQ=255)
                                             cccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                         <  1:2853699/100‑1 (MQ=255)
                                             cccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                         <  2:1599850/100‑1 (MQ=255)
                                              ccccccGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAGCG..GCAGGGTTTCCA.GCGGC.GTCCCTCCCCCCCCGGGGGCGGGGATCGAAAcccgccccg                                                         >  1:2049403/1‑93 (MQ=0)
                                               cccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGAt                                                       <  1:299709/101‑1 (MQ=255)
                                               cccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGAt                                                       <  1:3621762/101‑1 (MQ=255)
                                                   cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                                                   >  2:2836856/1‑101 (MQ=255)
                                                    gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                                  >  1:1235156/1‑101 (MQ=255)
                                                    gcaggggCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                                  >  1:2892064/4‑101 (MQ=255)
                                                     cgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcg                                                 >  2:1161170/1‑101 (MQ=255)
                                                      gggggGCTGGACCGAAATATCTGCGTGTAGTCGGGG..G..GCTGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                                                <  2:3835088/101‑1 (MQ=25)
                                                       ggggCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCa                                               >  2:3915629/1‑101 (MQ=255)
                                                        gggCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaa                                              <  2:351360/101‑1 (MQ=255)
                                                        gggCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaa                                              <  1:3254446/101‑1 (MQ=255)
                                                         ggCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaaa                                             >  2:521171/1‑101 (MQ=255)
                                                          gCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                                            <  2:2212242/101‑1 (MQ=255)
                                                            gTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                          >  1:1530876/1‑101 (MQ=255)
                                                            gTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACTGGTATGGATCTGGCGCAAACgg                                          >  2:479927/1‑101 (MQ=255)
                                                              ggATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                        <  1:4171172/101‑1 (MQ=255)
                                                              gcctCGAAACTCCTGCATGTAGGCGGCG..G..GCGGTTGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                        <  2:678962/98‑1 (MQ=37)
                                                               gATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                                       >  1:138248/1‑101 (MQ=255)
                                                                aTCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg                                      <  2:3590980/101‑1 (MQ=255)
                                                                aTCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg                                      <  2:1310250/101‑1 (MQ=255)
                                                                  cGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                                    >  2:2767760/1‑101 (MQ=255)
                                                                  cGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                                    >  2:4527218/1‑101 (MQ=255)
                                                                   gAAACATCTGCATGTAGTGGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGCCt                                   >  2:2138198/1‑101 (MQ=255)
                                                                   gAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                                   >  2:1940270/1‑101 (MQ=255)
                                                                   gAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                                   >  2:1896613/1‑101 (MQ=255)
                                                                   gAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                                   >  2:4286436/1‑101 (MQ=255)
                                                                    aaaCATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg                                  >  1:3585406/1‑101 (MQ=255)
                                                                         tCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                             >  2:2678765/1‑101 (MQ=255)
                                                                         tCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGCCTgccgtc                             >  2:3679848/1‑101 (MQ=255)
                                                                           tGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGc                           >  2:2260291/1‑101 (MQ=255)
                                                                              aTGTAGTCGGCG..G..GAAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                        <  1:3124245/101‑1 (MQ=255)
                                                                               ggTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAACGTCTGTCGTCGCTccc                       <  1:4413511/100‑1 (MQ=255)
                                                                                gTAGTCGGGG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc                      <  1:1161287/101‑1 (MQ=255)
                                                                                gTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc                      >  1:3087414/1‑101 (MQ=255)
                                                                                gTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc                      <  2:4599327/101‑1 (MQ=255)
                                                                                  aGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc                    >  1:73292/1‑101 (MQ=255)
                                                                                     cggcg..g..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgcg                 >  1:2091024/1‑101 (MQ=255)
                                                                                         g..g..gCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggggg             >  1:4889940/1‑101 (MQ=255)
                                                                                            g..gCGGTCCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGc            <  1:3888888/101‑1 (MQ=255)
                                                                                                 aGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg         <  1:323472/101‑1 (MQ=255)
                                                                                                  gTTCGA.GCCGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTgg        <  2:4180378/101‑1 (MQ=255)
                                                                                                   ttCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGa       <  1:4930463/101‑1 (MQ=255)
                                                                                                    tCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGAt      >  2:4982523/1‑101 (MQ=255)
                                                                                                    ccGA.GCCGCCGTCGCCCCCCCCGCGGGGGCGTTGATCGAAACTGGTATGGATCTGGCCCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGAt      <  1:2138346/100‑1 (MQ=38)
                                                                                                      gA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg    <  1:2328698/101‑1 (MQ=255)
                                                                                                         |                                                                                                    
CGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395593‑1395793

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG