Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,750 A→C 17.9% intergenic (+562/‑369) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,7500A→C17.9% 121.4 ‑1.1 ‑0.0290intergenic (+562/‑369)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/26):  ref base (110/105):  total (141/149)
Fisher's exact test strand distribution p-value = 3.26e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.12e-01

Only 100 of 305 total aligned reads displayed.

GGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGG  >  NC_014034/1395650‑1395850
                                                                                                        |                                                                                                          
ggggTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCaaa                                                                                                            <  1:3068081/98‑1 (MQ=255)
ggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCaaa                                                                                                            >  1:728828/1‑101 (MQ=255)
ggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCaaa                                                                                                            >  2:521171/1‑101 (MQ=255)
 gCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAAc                                                                                                           <  2:2212242/101‑1 (MQ=255)
   gTGGATCGAACCATCTGCATGTAGTGGGCGGGCGGTTGGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACgg                                                                                                         <  2:4046148/101‑1 (MQ=37)
     ggATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..aa                                                                                                     >  1:4133036/1‑101 (MQ=255)
     ggATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..aa                                                                                                     >  2:2285565/1‑101 (MQ=255)
     ggATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..aa                                                                                                     <  2:3976818/101‑1 (MQ=255)
      ggtCGAAGCATCTGGTGGTGGGCGGCGGGCGGTTGGAGCCGGAGTGGCGCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..aaa                                                                                                    <  1:3611064/99‑1 (MQ=21)
         cGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTc                                                                                                 >  1:516782/1‑101 (MQ=255)
         cGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGCATG.GATCTG..GCGCAAACGG..AAAGTc                                                                                                 >  2:291157/1‑101 (MQ=255)
          gAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCt                                                                                                >  2:1896613/1‑101 (MQ=255)
           aaaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g                                                                                              <  1:220889/101‑1 (MQ=255)
           aaaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g                                                                                              <  1:89681/101‑1 (MQ=255)
           aaaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g                                                                                              <  2:4403571/101‑1 (MQ=255)
              cATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g..tcg                                                                                         >  1:2817565/1‑101 (MQ=255)
                tCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGCCT.g..ccgtc                                                                                       >  2:3679848/1‑101 (MQ=255)
                  tGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGc                                                                                     >  2:2857642/1‑101 (MQ=255)
                     aTGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.cc                                                                                 >  1:3816673/1‑101 (MQ=255)
                       gTAGTCGGGGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.cccc                                                                               <  1:1161287/101‑1 (MQ=255)
                       gTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.cccc                                                                               <  2:4599327/101‑1 (MQ=255)
                       gTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.cccc                                                                               >  1:4734729/1‑101 (MQ=255)
                       gTAGTCGGCGGGAAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.cccc                                                                               <  2:4215985/101‑1 (MQ=255)
                          gTCGGGGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCcg                                                                            <  1:3210529/101‑1 (MQ=255)
                           tgggggggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCcgc                                                                           <  2:4502216/96‑1 (MQ=255)
                             ggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCgg                                                                         >  2:2121547/1‑101 (MQ=255)
                             ggcggGCAGTGCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCgg                                                                         <  2:3864247/101‑1 (MQ=255)
                                 ggCGGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGc                                                                     <  2:3378504/101‑1 (MQ=255)
                                  gCAGTGCGAGCCGCAGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCCGCGGGGGc                                                                     <  2:2617497/101‑1 (MQ=38)
                                   cAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGt                                                                   <  1:2690571/101‑1 (MQ=255)
                                    aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCN.CCCCCCGCGGGGGCGTg                                                                  >  2:1196052/1‑101 (MQ=255)
                                     gTTCGAGCCGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTgg                                                                 <  2:4180378/101‑1 (MQ=255)
                                      ttCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGa                                                                <  1:4930463/101‑1 (MQ=255)
                                       tCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGAt                                                               >  1:2637204/1‑101 (MQ=255)
                                        caagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCT.CCCCCCGCGGGGGCGTgg                                                                 <  1:4882990/94‑1 (MQ=2)
                                        cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATc                                                              <  2:721739/101‑1 (MQ=255)
                                         gcgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCT.CCCCCCGCGGGGGCGTGGAt                                                               <  1:98905/93‑1 (MQ=0)
                                         aagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCT.CCCCCCGCGGGGGCGTGGa                                                                <  1:3662728/95‑1 (MQ=2)
                                           gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCg                                                             <  1:3977772/95‑1 (MQ=0)
                                           gattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..GGGTCGCT.CCCCCCGCGGGGGCGTGGATc                                                              >  2:506593/5‑101 (MQ=2)
                                           atctcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCT.CCCCCCGCGGGGGCGTGGATc                                                              <  1:4612303/97‑1 (MQ=2)
                                            ccGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                          <  1:4527538/101‑1 (MQ=255)
                                            attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCT.CCCCCCGCGGGGGCGTGGATCg                                                             <  1:4809219/98‑1 (MQ=2)
                                              tcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                           >  2:3778276/2‑101 (MQ=2)
                                              ctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                          <  2:4923984/98‑1 (MQ=2)
                                               tggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAc                                                         <  2:1387470/99‑1 (MQ=2)
                                               cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCt                                                       <  1:1941386/101‑1 (MQ=255)
                                                ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAcc                                                        <  1:1258730/100‑1 (MQ=2)
                                                aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAc                                                         <  2:4756481/101‑1 (MQ=2)
                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAca                                                        >  1:1683291/1‑100 (MQ=2)
                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAca                                                        <  1:14932/101‑2 (MQ=2)
                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCt                                                       <  2:4361923/101‑1 (MQ=2)
                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAcac                                                       <  1:2535894/101‑3 (MQ=2)
                                                  gcGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.ATGACCAAAT..CCGCAATCGG..CCGGGCT.GGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCta                                                      <  1:4264405/100‑2 (MQ=2)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAcaccc                                                     >  2:4984721/1‑97 (MQ=2)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                    <  1:155601/101‑4 (MQ=2)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                    <  1:1057341/101‑4 (MQ=2)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                    >  1:378423/1‑98 (MQ=2)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTgcggt                                                  <  2:4565843/101‑2 (MQ=255)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTgcggc                                                  <  1:618059/101‑1 (MQ=255)
                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAcacccg                                                    <  2:4893434/101‑6 (MQ=2)
                                                      tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                                  <  1:3037247/101‑6 (MQ=2)
                                                      tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                                  >  2:3106645/1‑96 (MQ=2)
                                                      tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                                  >  1:2439499/1‑96 (MQ=2)
                                                      tCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTgcggcgg                                                >  2:1599849/1‑100 (MQ=255)
                                                       ccccccGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                                  >  1:2159312/1‑94 (MQ=0)
                                                       ccccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                               <  2:1530746/101‑1 (MQ=255)
                                                       ccccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                               >  1:3330049/1‑101 (MQ=255)
                                                       ccccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                               >  1:84409/1‑101 (MQ=255)
                                                        cccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGa                                              <  2:3727125/101‑1 (MQ=255)
                                                        cccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT..CCCCCGCGGGGGCGTGGATCGAAACCCGCGGCGGGAg                                             >  2:3491625/1‑101 (MQ=255)
                                                          cccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTTAGt                                            <  2:4274163/101‑1 (MQ=255)
                                                           ccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGtt                                           <  1:133561/101‑1 (MQ=255)
                                                            cgcgGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAgatc                                          >  2:5017295/1‑98 (MQ=255)
                                                            cgcgGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTg                                          <  1:3435277/101‑1 (MQ=255)
                                                              cgGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGcc                                        <  1:2679592/101‑1 (MQ=255)
                                                               gggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                                       >  1:2319675/1‑101 (MQ=255)
                                                                ggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                                      >  2:1964466/1‑101 (MQ=255)
                                                                   gCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGa                                   <  2:2694452/101‑1 (MQ=255)
                                                                    cGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGAt                                  <  1:4326667/101‑1 (MQ=255)
                                                                    cGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGAt                                  <  1:2384191/101‑1 (MQ=255)
                                                                     gTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATa                                 >  2:2752649/1‑101 (MQ=255)
                                                                      gggATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT..CCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGa                               <  1:3491890/100‑1 (MQ=255)
                                                                         aTCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg                             <  1:444478/101‑1 (MQ=255)
                                                                           cGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGcc                           <  1:2080246/101‑1 (MQ=255)
                                                                            gAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCg                          >  2:2298444/1‑101 (MQ=255)
                                                                             aaaCT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCGGTGATAGACGCCGt                         >  1:4973808/1‑101 (MQ=255)
                                                                             aaaCT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGt                         >  2:1840929/1‑101 (MQ=255)
                                                                             aaaCT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGt                         >  2:3326507/1‑101 (MQ=255)
                                                                               aCT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGCGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTgg                       >  1:2971400/1‑101 (MQ=255)
                                                                                 t.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGAc                     >  1:1873195/1‑101 (MQ=255)
                                                                                 t.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGAc                     <  1:3282583/101‑1 (MQ=255)
                                                                                    gTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGt                   <  2:3621307/101‑1 (MQ=255)
                                                                                     tATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTc                  <  2:4305053/101‑1 (MQ=255)
                                                                                      aTG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCg                 <  2:2016822/101‑1 (MQ=255)
                                                                                           aTCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcc             >  2:2225371/1‑101 (MQ=255)
                                                                                               g..gCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccccc         >  2:4673375/1‑101 (MQ=255)
                                                                                               g..gCGCAAACGG..AAAGTCT.G..TCGTCGCC.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccccc         <  1:2115748/101‑1 (MQ=255)
                                                                                                       aaCGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCgggg   <  2:3585135/101‑1 (MQ=255)
                                                                                                       aaCGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTAAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCgggg   <  1:4029958/101‑1 (MQ=255)
                                                                                                        |                                                                                                          
GGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGG  >  NC_014034/1395650‑1395850

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG