Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,816 G→C 3.3% intergenic (+628/‑303) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8160G→C 3.3% 10.6 ‑0.7 ‑0.1243intergenic (+628/‑303)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/5):  ref base (124/111):  total (127/116)
Fisher's exact test strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.88e-01

Only 100 of 261 total aligned reads displayed.

GGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGG  >  NC_014034/1395716‑1395916
                                                                                                     |                                                                                                      
 gCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGa                                                                                                       <  2:2694452/101‑1 (MQ=255)
   gTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATa                                                                                                     >  2:1974341/1‑101 (MQ=255)
   gTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATa                                                                                                     >  2:2752649/1‑101 (MQ=255)
   gTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATa                                                                                                     <  2:923144/101‑1 (MQ=255)
    tGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTTCG.GCGTGAGTTGCCAGTGATAg                                                                                                    <  2:3948830/101‑1 (MQ=255)
    tGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAg                                                                                                    <  1:1196169/101‑1 (MQ=255)
    tGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAg                                                                                                    >  1:728176/1‑101 (MQ=255)
    gggATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCT.CCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGa                                                                                                   <  1:3491890/100‑1 (MQ=255)
      gATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.c                                                                                                 <  2:4838469/101‑1 (MQ=255)
       aTCGAAAATGGAATGGATCAGGCGCAAACGGTAAGTCTGTCGTCGGTCCCCCCGCGGGGGCGTGGATCGAAGCACGCG.ACGGGAGGTCCCAGCGATAGagg                                                                                                 >  2:2443276/1‑99 (MQ=16)
           aaaCTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCGGTGATAGA.CGCCGt                                                                                            >  1:4973808/1‑101 (MQ=255)
           aaaCTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGt                                                                                            >  2:1840929/1‑101 (MQ=255)
             aCTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTgg                                                                                          >  1:1677645/1‑101 (MQ=255)
             aCTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTgg                                                                                          >  1:2971400/1‑101 (MQ=255)
               tGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCAGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.c                                                                                       >  1:4332930/1‑101 (MQ=255)
                 gTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGt                                                                                     <  2:3621307/101‑1 (MQ=255)
                  tATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTc                                                                                    >  1:2558902/1‑101 (MQ=255)
                   aTGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.Cgctg                                                                                   >  1:2485892/1‑98 (MQ=255)
                     ggATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCt                                                                                 >  1:3807805/1‑101 (MQ=255)
                     ggATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCt                                                                                 <  2:4362772/101‑1 (MQ=255)
                       aTCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcc                                                                               >  2:2225371/1‑101 (MQ=255)
                       aTCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcc                                                                               <  1:3425773/101‑1 (MQ=255)
                            gcgcAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTccccccc                                                                          >  2:352936/1‑100 (MQ=255)
                               cAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                                                                       >  2:3678384/1‑101 (MQ=255)
                                aaaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggg                                                                      >  1:267426/1‑101 (MQ=255)
                                 aaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTAAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgggg                                                                     <  1:4029958/101‑1 (MQ=255)
                                   cGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggggcc                                                                   >  2:2355307/1‑99 (MQ=255)
                                   cGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGc                                                                   >  1:4783394/1‑101 (MQ=255)
                                   cGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGc                                                                   >  1:1614343/1‑101 (MQ=255)
                                   cGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGc                                                                   <  1:1008604/101‑1 (MQ=255)
                                    ggAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCg                                                                  >  1:3101266/1‑101 (MQ=255)
                                     gAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCgg                                                                 >  1:1446326/1‑100 (MQ=255)
                                     gAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCgg                                                                 >  2:4495520/1‑100 (MQ=255)
                                     gAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGt                                                                 <  2:4889613/101‑1 (MQ=255)
                                      ggccccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                                                 <  1:20309/92‑1 (MQ=0)
                                      ggccccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                                                 <  2:2209777/92‑1 (MQ=0)
                                      ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                                                 <  2:3042928/92‑1 (MQ=0)
                                       aaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                               <  1:3665525/101‑1 (MQ=255)
                                       aaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                               <  2:3329786/101‑1 (MQ=255)
                                       aaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                               >  1:3237075/1‑101 (MQ=255)
                                        aGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGC.CGCCGTGGA.CGCCGCTCCCCCCCGGGCGGCGTGGa                                                              >  2:4203924/1‑101 (MQ=37)
                                         gTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgggt                                                             >  1:4543037/1‑99 (MQ=255)
                                          tCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            >  2:647316/1‑101 (MQ=255)
                                          tCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGGGATAGA.CGCCGTGGA.CGTCGCCCCCCCCCCGGGGGCGTGGATc                                                            >  1:1781594/1‑101 (MQ=255)
                                           cTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           <  2:253847/101‑1 (MQ=255)
                                           cTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           <  1:4212407/101‑1 (MQ=255)
                                           cTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           >  2:72287/1‑101 (MQ=255)
                                            tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                          >  1:2872901/1‑101 (MQ=255)
                                             gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         >  1:126455/1‑101 (MQ=255)
                                             gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         >  1:3863081/1‑101 (MQ=255)
                                              tcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                        <  1:3326770/101‑1 (MQ=255)
                                               cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       <  1:2260446/101‑1 (MQ=255)
                                                gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCC.GCGTGGGTTGACAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGGGTGGATCGAAAcc                                                      >  2:2104167/1‑100 (MQ=37)
                                                 tcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                     >  1:1564179/1‑101 (MQ=255)
                                                 tcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                     <  2:3144027/101‑1 (MQ=255)
                                                 tcgCCCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                     <  1:1085362/101‑1 (MQ=255)
                                                  cgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTGGCTCCCCCCGCGGGGGCGTGGATCGAAACAgc                                                    >  2:1535704/1‑101 (MQ=255)
                                                  cgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgc                                                    >  1:4918954/1‑101 (MQ=255)
                                                  cgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgc                                                    <  1:2986346/101‑1 (MQ=255)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcg                                                   >  1:3373364/1‑101 (MQ=255)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcg                                                   <  1:984384/101‑1 (MQ=255)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcg                                                   >  2:1627830/1‑101 (MQ=255)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGCGGGCGTGGCTCGAAACCgcgc                                                  >  1:2328476/1‑101 (MQ=255)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgcc                                                 >  1:2051691/1‑101 (MQ=255)
                                                      ccccccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgccg                                                >  2:427742/1‑101 (MQ=255)
                                                        ccccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCgccgcc                                              <  1:4993005/101‑1 (MQ=255)
                                                        ccccGCGGGGCCGTGGATGGAAGCCTGCG.GGGGGAGGTGCCAGTGATAGA.CGCCGTGGA.CGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCgccgcc                                              <  2:812530/101‑1 (MQ=37)
                                                           cgcgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAt                                           <  2:1235039/101‑1 (MQ=255)
                                                           cgcgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAt                                           <  1:55451/101‑1 (MQ=255)
                                                            gcgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATc                                          >  1:4697450/1‑101 (MQ=255)
                                                            gcgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATc                                          <  1:4155781/101‑1 (MQ=255)
                                                              gggggCGTGGATGGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGc                                        >  1:2742933/1‑101 (MQ=255)
                                                              gggggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGc                                        <  1:2767962/101‑1 (MQ=255)
                                                               ggggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCa                                       >  1:2016962/1‑101 (MQ=255)
                                                               ggggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCa                                       >  2:2291396/1‑101 (MQ=255)
                                                                gggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                      <  2:4504006/101‑1 (MQ=255)
                                                                 ggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                                     >  1:1840181/1‑101 (MQ=255)
                                                                    gTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                  >  1:2706066/1‑101 (MQ=255)
                                                                        aTCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGCA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGa                              >  2:1595270/1‑101 (MQ=255)
                                                                         tCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAg                             <  2:2872688/101‑1 (MQ=255)
                                                                         tCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAg                             <  2:4036782/101‑1 (MQ=255)
                                                                          cGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAgc                            >  2:4398083/1‑101 (MQ=255)
                                                                               ccTGCG.GCGTGAGTTGCTAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGcgccgc                       <  2:4545323/101‑1 (MQ=255)
                                                                               ccTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGcgccgc                       >  2:456749/1‑101 (MQ=255)
                                                                                 tGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCgcgc                     <  2:1773835/101‑1 (MQ=255)
                                                                                  gcg.gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCgcgcc                    >  2:4222118/1‑100 (MQ=255)
                                                                                   cg.gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAg                   >  2:3830589/1‑101 (MQ=255)
                                                                                    g.gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGt                  >  1:4412947/1‑101 (MQ=255)
                                                                                      gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTc                 >  1:3620172/1‑101 (MQ=255)
                                                                                      gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTc                 >  1:2107206/1‑101 (MQ=255)
                                                                                       cgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGC.GGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGc               >  2:2928422/1‑101 (MQ=255)
                                                                                        gTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGc               >  2:1119825/1‑101 (MQ=255)
                                                                                             ttGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcccc          >  1:398629/1‑101 (MQ=255)
                                                                                              tGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTccccc         >  2:3392486/1‑101 (MQ=255)
                                                                                              tGCCAGTGATAGA.CGCCGGGGA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTccccc         <  2:2319511/101‑1 (MQ=255)
                                                                                               gCCAGTGATAGC.CGCCGTGGC.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcccccc        <  1:4118800/101‑1 (MQ=255)
                                                                                               gCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcccccc        <  2:3266435/101‑1 (MQ=255)
                                                                                                gcAGTGATAGG.CGCCGTGGA.CGTCGCTCCCCACGCGGGGGCGTGAATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCcg       <  2:4600809/100‑1 (MQ=255)
                                                                                                 cAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCcgc      <  2:2048089/101‑1 (MQ=255)
                                                                                                 cAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCcgc      >  2:1690558/1‑101 (MQ=255)
                                                                                                     |                                                                                                      
GGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGG  >  NC_014034/1395716‑1395916

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG