Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,874 A→C 23.0% intergenic (+686/‑245) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8740A→C23.0% 199.5 ‑1.1 ‑0.2311intergenic (+686/‑245)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (29/43):  ref base (113/126):  total (142/169)
Fisher's exact test strand distribution p-value = 3.45e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.74e-01

Only 100 of 327 total aligned reads displayed.

CCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCC  >  NC_014034/1395774‑1395974
                                                                                                      |                                                                                                          
ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTa                                                                                                            <  1:4982853/101‑1 (MQ=255)
  gcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATc                                                                                                          <  1:4155781/101‑1 (MQ=255)
  gcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATc                                                                                                          <  2:2426807/101‑1 (MQ=255)
   cgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCg                                                                                                         <  2:352847/101‑1 (MQ=255)
   cgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCg                                                                                                         <  1:1080232/101‑1 (MQ=255)
    gggggCGTGGATGGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGc                                                                                                        >  1:2742933/1‑101 (MQ=255)
    gggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGc                                                                                                        >  1:4826520/1‑101 (MQ=255)
      gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                      <  1:4059614/101‑1 (MQ=255)
      gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                      <  2:1613152/101‑1 (MQ=255)
       ggCGTGGATCGAACCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATc                                                                                                     <  2:1050775/101‑1 (MQ=255)
        gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCG.CCGC.CTATCCCATcc                                                                                                    >  2:311189/1‑101 (MQ=255)
          gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                                                                  >  1:2706066/1‑101 (MQ=255)
            ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...Tc                                                                                             >  1:29682/1‑101 (MQ=255)
             gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCg                                                                                            >  2:1785872/1‑101 (MQ=255)
               tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAg                                                                                          <  2:2872688/101‑1 (MQ=255)
                     ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGcgcc.gc                                                                                   >  2:456749/1‑101 (MQ=255)
                        gcggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.Ca                                                                               <  2:2891851/101‑1 (MQ=255)
                          ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.gc                                                                            >  2:1531266/1‑100 (MQ=255)
                          ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.Gt                                                                            >  2:3655567/1‑101 (MQ=255)
                          ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.Gt                                                                            >  1:4412947/1‑101 (MQ=255)
                            cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCg                                                                          >  1:3933280/1‑101 (MQ=255)
                                  ttGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTcccc                                                                    >  1:398629/1‑101 (MQ=255)
                                  tggCCAGTGATGGGCGCGGTGGCCGCCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTcccc                                                                    <  2:4542717/99‑1 (MQ=37)
                                   tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTccccc                                                                   >  2:3392486/1‑101 (MQ=255)
                                   tGCCAGTGATAGACGCCGGGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTccccc                                                                   <  2:2319511/101‑1 (MQ=255)
                                    gCCGGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTcccccc                                                                  <  2:1639990/101‑1 (MQ=255)
                                    gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTcccccc                                                                  <  1:1794362/101‑1 (MQ=255)
                                      cAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcgc                                                                <  2:2048089/101‑1 (MQ=255)
                                      cAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcgc                                                                >  2:1690558/1‑101 (MQ=255)
                                         tgggaGACGCCGTGGCCGTCCCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCggg                                                             <  2:4684181/97‑1 (MQ=38)
                                             aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                         >  1:116263/1‑101 (MQ=255)
                                              ggagcctgggACTTCCCTCCCCGCGGGGGGGCGTGGAGTGAACCTGCG.CCGC.CGTTCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGt                                                        <  1:2443450/93‑1 (MQ=25)
                                              gACGCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGt                                                        <  2:2733975/101‑1 (MQ=255)
                                                gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTgg                                                      <  1:2784121/91‑1 (MQ=9)
                                                gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTgg                                                      <  2:4041682/91‑1 (MQ=9)
                                                 cggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCCGCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGt                                                        <  2:3328091/93‑1 (MQ=0)
                                                  ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTg                                                       >  2:3147431/8‑101 (MQ=2)
                                                  ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTg                                                       <  1:125826/94‑1 (MQ=2)
                                                  ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTg                                                       >  1:2387001/8‑101 (MQ=2)
                                                  ccGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  2:4918627/101‑1 (MQ=255)
                                                   catcgccGTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                   >  2:514793/7‑101 (MQ=9)
                                                   cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  2:1564049/101‑1 (MQ=255)
                                                   cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  1:228874/101‑1 (MQ=255)
                                                   aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  1:4506738/94‑1 (MQ=9)
                                                    gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  >  2:2675619/1‑101 (MQ=255)
                                                    gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  >  1:3196035/1‑101 (MQ=255)
                                                    ccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  2:951494/96‑1 (MQ=2)
                                                    acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  >  1:1389056/7‑101 (MQ=9)
                                                    acctatgTCGCGCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  <  2:675789/95‑1 (MQ=9)
                                                      ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                >  1:3080000/1‑101 (MQ=255)
                                                      ggACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                <  1:2595230/101‑1 (MQ=255)
                                                      ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                <  1:4618729/97‑1 (MQ=9)
                                                      ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                >  2:4462549/5‑101 (MQ=9)
                                                       gACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                               <  2:4973478/101‑1 (MQ=255)
                                                         tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                             >  1:3384980/2‑100 (MQ=9)
                                                         cggcGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                             <  1:4222435/98‑1 (MQ=255)
                                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                            >  1:4126931/1‑101 (MQ=255)
                                                          gTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaa                                            <  2:140271/101‑3 (MQ=9)
                                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                           >  1:2887474/1‑101 (MQ=255)
                                                           tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                           <  2:4806078/101‑1 (MQ=9)
                                                           tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                           <  1:578051/101‑1 (MQ=9)
                                                              cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                        >  2:4194280/1‑101 (MQ=9)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                                       >  1:2017597/1‑101 (MQ=255)
                                                                ccccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCggt                                      >  2:2218036/1‑99 (MQ=255)
                                                                caccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACAATGCggc                                      >  2:144832/3‑99 (MQ=9)
                                                                 cccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                     <  1:1531396/101‑1 (MQ=255)
                                                                 cccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                     <  2:2267372/101‑1 (MQ=255)
                                                                  ccccGCGGGGGCGTGTATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                    <  2:2536248/101‑1 (MQ=255)
                                                                  ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                    <  1:74237/101‑1 (MQ=255)
                                                                    ccGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                                     <  2:260237/101‑10 (MQ=2)
                                                                     cgcgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCGCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                 >  2:3261997/1‑101 (MQ=255)
                                                                      gcgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGGGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGc                                <  1:1896750/101‑1 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                               <  1:1009221/101‑10 (MQ=2)
                                                                        gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                              <  2:1734095/101‑11 (MQ=2)
                                                                        gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGCGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGACGCGATCATGGCCt                              >  2:4275150/1‑101 (MQ=255)
                                                                        gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                              >  2:1140408/1‑101 (MQ=255)
                                                                         ggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                             <  1:1841062/101‑1 (MQ=255)
                                                                          gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                            <  2:1829275/101‑1 (MQ=255)
                                                                          gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                            <  2:3862789/101‑1 (MQ=255)
                                                                           ggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                           <  1:427807/101‑1 (MQ=255)
                                                                           ggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                           <  1:352989/101‑1 (MQ=255)
                                                                           ggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCCACg                           >  2:888358/1‑101 (MQ=255)
                                                                            gCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                          <  1:3779556/101‑1 (MQ=255)
                                                                              gTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                        >  1:860014/1‑101 (MQ=255)
                                                                                ggATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                      >  1:2185434/1‑101 (MQ=255)
                                                                                 gATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc                     <  2:4006879/101‑1 (MQ=255)
                                                                                   tCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAg                   <  2:4391529/101‑1 (MQ=255)
                                                                                     gAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                 <  1:2331528/101‑1 (MQ=255)
                                                                                     gAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                 >  1:3671290/1‑101 (MQ=255)
                                                                                       aaCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa               <  2:1357382/101‑1 (MQ=255)
                                                                                          tGCG.CCGC.CTAACGCATCCGG...TCGAGCGCC.GCG.CA.GGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg            <  2:793240/101‑1 (MQ=255)
                                                                                            cg.ccgc.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt          <  2:3101028/101‑1 (MQ=255)
                                                                                              cccgc.cTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc         <  2:3688301/100‑1 (MQ=255)
                                                                                                cgc.cTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGc       <  1:21109/101‑1 (MQ=255)
                                                                                                cgc.cTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGc       <  1:2010137/101‑1 (MQ=255)
                                                                                                 gc.cTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt      <  1:1627962/101‑1 (MQ=255)
                                                                                                 cc.cTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt      <  2:376160/100‑1 (MQ=255)
                                                                                                    cTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc    >  1:5029718/1‑101 (MQ=255)
                                                                                                    cTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc    <  1:2857855/101‑1 (MQ=255)
                                                                                                     tATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc   <  2:3079762/101‑1 (MQ=255)
                                                                                                      |                                                                                                          
CCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCC  >  NC_014034/1395774‑1395974

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG