BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,956	A→C	2.5%	intergenic (+768/‑163)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,956	0	A→C	2.5%	6.1 ‑0.8 ‑0.1	238	intergenic (+768/‑163)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (2/4): ref base (122/109): total (124/114)
Fisher's exact test strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.42e-01

Only 100 of 249 total aligned reads displayed.


TCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1395857‑1396056


  gAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTg                                                                                                   >  1:3671290/1‑101 (MQ=255)


  gAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTg                                                                                                   <  1:2331528/101‑1 (MQ=255)


  gAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTg                                                                                                   <  1:2051392/101‑1 (MQ=255)


   aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTgg                                                                                                  <  2:398564/101‑1 (MQ=255)


   aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTgg                                                                                                  >  1:3130263/1‑101 (MQ=255)


    aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGa                                                                                                 <  2:1357382/101‑1 (MQ=255)


    aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGa                                                                                                 <  2:3327301/101‑1 (MQ=255)


    aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGa                                                                                                 >  1:1678944/1‑101 (MQ=255)


    aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTAGa                                                                                                 <  1:2884298/101‑1 (MQ=255)


      cTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACt                                                                                               <  2:2652816/101‑1 (MQ=255)


          gccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTc                                                                                           <  2:4388267/101‑1 (MQ=255)


          cccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTc                                                                                           <  2:3688301/100‑1 (MQ=255)


           ccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCg                                                                                          >  1:4976927/1‑101 (MQ=255)


           ccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGGCg                                                                                          >  2:2622865/1‑101 (MQ=255)


             cccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt                                                                                        <  2:376160/100‑1 (MQ=255)


                 aTCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc                                                                                    >  2:31217/1‑101 (MQ=255)


                  tCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccccc                                                                                   >  1:3356076/1‑101 (MQ=255)


                  tCGCATCCGGTCGAGCGCCGCGCAGTCGCTACCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGGCGCTccccc                                                                                   >  1:4492324/1‑101 (MQ=255)


                   cGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                                                                                  <  1:2775648/101‑1 (MQ=255)


                    gCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGGGGACTGGGCGCCccccccc                                                                                 >  1:3460235/1‑100 (MQ=38)


                        ccGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg                                                                             >  1:3830483/1‑101 (MQ=255)


                          ggTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg                                                                           >  1:56790/1‑101 (MQ=255)


                           gTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc                                                                          <  1:96089/101‑1 (MQ=255)


                           gTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc                                                                          >  1:1290091/1‑101 (MQ=255)


                           gTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCCCGGGGGc                                                                          >  1:4415115/1‑101 (MQ=255)


                            tCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCg                                                                         <  1:3987524/101‑1 (MQ=255)


                             cGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGt                                                                        <  2:2985292/101‑1 (MQ=255)


                             cGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGt                                                                        >  2:2756360/1‑101 (MQ=255)


                              gAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCCGTGGCCTGGTCGCCCCCCCCGCGGGGGCggg                                                                       >  1:1194560/1‑99 (MQ=38)


                                 cgccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    <  1:1794124/101‑1 (MQ=255)


                                  gccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:1140522/101‑1 (MQ=255)


                                  gccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GACCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   >  2:4366721/1‑101 (MQ=255)


                                   ccgcGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  1:4599193/101‑1 (MQ=255)


                                     gcgcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                <  2:29672/101‑1 (MQ=255)


                                     catccatCGCCCCGCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                >  2:3500882/7‑101 (MQ=37)


                                      ggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGC..TGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGGTCGaaa                                                               >  2:328716/5‑101 (MQ=2)


                                      cgcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               <  1:1599981/101‑1 (MQ=255)


                                       gcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              <  1:2553952/101‑1 (MQ=255)


                                       gcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              >  2:2841088/1‑101 (MQ=255)


                                        ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                             <  1:83273/99‑1 (MQ=255)


                                        cAGTCGCTCCCACCGCGGGGGCGTGGATCGAAGCGCGGCAAGCACATCGCCGATCTGGGTAAGGCCGGTGGTCGCTCCCCCCGCGGGGGCTTCGATCGaaa                                                               <  2:308898/101‑1 (MQ=2)


                                        atgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGC..TGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGGGGGTCGAAAcg                                                             >  1:2967903/3‑100 (MQ=2)


                                         aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTc                                                            <  2:1831864/101‑1 (MQ=255)


                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCg                                                           >  2:2239026/1‑101 (MQ=255)


                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGcc                                                         >  1:1997426/1‑101 (MQ=255)


                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGcc                                                         >  1:1620817/1‑101 (MQ=255)


                                               tCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTGGGGCAGCGGACGGGTCGCCACCCCCGCGGGGGCGTCGATCGAAAGCCGcccac                                                      >  2:5027274/1‑99 (MQ=25)


                                               tCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTc                                                      <  1:2854932/101‑1 (MQ=255)


                                               gccccccGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTc                                                      <  2:1687711/100‑1 (MQ=255)


                                                ccccccGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCg                                                     >  2:1998617/1‑101 (MQ=255)


                                                 cccccGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGa                                                    <  1:4855296/101‑1 (MQ=255)


                                                      gcgGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcgc                                               >  1:2095565/1‑101 (MQ=255)


                                                       cgGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcgcg                                              >  2:1852836/1‑101 (MQ=255)


                                                         ggggCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                                            >  1:3607684/1‑101 (MQ=255)


                                                         ggggCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                                            >  1:4152917/1‑101 (MQ=255)


                                                         ggggCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGAAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                                            >  2:4063597/1‑101 (MQ=255)


                                                          gggCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCtt                                           <  1:2182988/101‑1 (MQ=255)


                                                            gCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGc                                         <  1:1013914/101‑1 (MQ=255)


                                                             cGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGcc                                        >  2:2286639/1‑101 (MQ=255)


                                                              gTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                                       >  1:4872491/1‑101 (MQ=255)


                                                              gTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                                       <  2:56780/101‑1 (MQ=255)


                                                              gTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                                       >  1:732087/1‑101 (MQ=255)


                                                               tGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                                      <  2:2869657/101‑1 (MQ=255)


                                                                ggATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                                     >  1:2932487/1‑101 (MQ=255)


                                                                 gATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                                    >  2:3207779/1‑101 (MQ=255)


                                                                 gATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                                    <  2:4532085/101‑1 (MQ=255)


                                                                  aTCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg                                   >  2:4415350/1‑101 (MQ=255)


                                                                   tCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGc                                  <  1:1041999/101‑1 (MQ=255)


                                                                    cGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGcc                                 >  2:10763/1‑101 (MQ=255)


                                                                    cGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGcc                                 >  1:987302/1‑101 (MQ=255)


                                                                    cGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGcc                                 >  1:191017/1‑101 (MQ=255)


                                                                     gAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                                >  2:1564135/1‑101 (MQ=255)


                                                                     gAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                                >  1:3080522/1‑101 (MQ=255)


                                                                      aaaGGATGC..GTTCATGGCCTACGGCGGGCAGTGGACTGGTCGCTCCCCCCGTGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg                               <  1:4275467/101‑1 (MQ=255)


                                                                      aaaCGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg                               <  1:170377/101‑1 (MQ=255)


                                                                        aCGATGG..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGcc                             <  1:719728/101‑1 (MQ=255)


                                                                        aCGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGcc                             <  2:2518832/101‑1 (MQ=255)


                                                                         cGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcg                            >  2:3073563/1‑101 (MQ=255)


                                                                         cGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcg                            <  1:4381923/101‑1 (MQ=255)


                                                                           aTGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcgct                          >  2:4951606/1‑100 (MQ=255)


                                                                            tGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGt                         <  1:228689/101‑1 (MQ=255)


                                                                             gC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTc                        >  1:1197788/1‑101 (MQ=255)


                                                                              c..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCg                       >  2:544082/1‑101 (MQ=255)


                                                                              c..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCg                       <  2:4836104/101‑1 (MQ=255)


                                                                                 gTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGc                      >  2:816159/1‑101 (MQ=255)


                                                                                   tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc                    >  1:1413128/1‑101 (MQ=255)


                                                                                    cATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTcc                   >  1:475488/1‑101 (MQ=255)


                                                                                    cATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTcc                   >  1:1375475/1‑101 (MQ=255)


                                                                                     aTGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTccc                  <  2:731998/101‑1 (MQ=255)


                                                                                      tGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTcccc                 >  1:1492346/1‑101 (MQ=255)


                                                                                       ggCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTccccc                <  2:4211254/101‑1 (MQ=255)


                                                                                       ggCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTccccc                <  2:4269668/101‑1 (MQ=255)


                                                                                        gCCTACGTCGGGCAGGGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTcccccc               <  2:3459970/101‑1 (MQ=255)


                                                                                          cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCcgc             >  2:4433299/1‑101 (MQ=255)


                                                                                           tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCcgcg            >  1:661131/1‑101 (MQ=255)


                                                                                            aCGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCCCCCCCCGCgg           <  1:4367038/101‑1 (MQ=255)


                                                                                             cGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCggg          >  2:2747184/1‑101 (MQ=255)


                                                                                              gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCgggg         >  1:2618753/1‑101 (MQ=255)


                                                                                                 gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCg      <  2:2166966/101‑1 (MQ=255)


                                                                                                     aGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTggg  >  1:3026338/1‑100 (MQ=255)


TCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1395857‑1396056

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG