Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,013 C→T 13.9% intergenic (+825/‑106) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0130C→T13.9% 101.6 ‑1.2 ‑0.3295intergenic (+825/‑106)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (17/24):  ref base (128/126):  total (145/150)
Fisher's exact test strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.87e-01

Only 100 of 315 total aligned reads displayed.

GGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGC  >  NC_014034/1395913‑1396112
                                                                                                    |                                                                                                        
gggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCgcgc                                                                                                          <  1:1119939/101‑1 (MQ=255)
 ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                                                                                                         >  1:3607684/1‑101 (MQ=255)
 ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGAAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                                                                                                         >  2:4063597/1‑101 (MQ=255)
  gggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCtt                                                                                                        >  2:3262068/1‑101 (MQ=255)
    gCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGc                                                                                                      <  1:1013914/101‑1 (MQ=255)
      gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                                                                                                    <  2:56780/101‑1 (MQ=255)
      gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                                                                                                    >  2:121496/1‑101 (MQ=255)
       tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                                                                                                   >  2:834981/1‑101 (MQ=255)
       tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                                                                                                   >  1:1180577/1‑101 (MQ=255)
       tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                                                                                                   <  2:2869657/101‑1 (MQ=255)
       tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                                                                                                   >  1:3727840/1‑101 (MQ=255)
       tGGATCGAAACGAGGCGTTCATGGCCTACGTCGGGCAGTGCACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                                                                                                   <  2:4146794/101‑1 (MQ=255)
         naTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..Ac                                                                                               >  1:150464/2‑101 (MQ=255)
         gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..Ac                                                                                               >  2:3207779/1‑101 (MQ=255)
            cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGcc                                                                                            >  1:987302/1‑101 (MQ=255)
            cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGcc                                                                                            >  1:191017/1‑101 (MQ=255)
             gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCt                                                                                           >  1:1292857/1‑101 (MQ=255)
              aaaGGATGCGTTCATGGCCTACGGCGGGCAGTGGACTGGTCGCTCCCCCCGTGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTg                                                                                          <  1:4275467/101‑1 (MQ=255)
                  gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.cgc                                                                                     >  1:736143/1‑101 (MQ=255)
                   aTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.cgct                                                                                    >  2:4951606/1‑100 (MQ=255)
                     gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTc                                                                                <  2:1446199/101‑1 (MQ=255)
                     gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTc                                                                                <  1:4367512/101‑1 (MQ=255)
                      cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCg                                                                               >  2:544082/1‑101 (MQ=255)
                         tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTc                                                                            >  1:637735/1‑101 (MQ=255)
                         tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTc                                                                            >  2:4467914/1‑101 (MQ=255)
                           aTGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTccc                                                                          >  1:2755161/1‑101 (MQ=255)
                             ggCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTccccc                                                                        <  2:4269668/101‑1 (MQ=255)
                             ggCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTccccc                                                                        >  1:2956853/1‑101 (MQ=255)
                              gCCTACGTCGGGCAGGGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTcccccc                                                                       <  2:3459970/101‑1 (MQ=255)
                              gCCTACGGCGGGCAGTGGACTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTcccccc                                                                       <  1:1412277/101‑1 (MQ=255)
                               ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCcg                                                                      <  1:4007528/101‑1 (MQ=255)
                                cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCcgc                                                                     <  2:19189/101‑1 (MQ=255)
                                 tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                    >  2:4411338/1‑101 (MQ=255)
                                    gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCgggg                                                                 >  1:2618753/1‑101 (MQ=255)
                                    gTCGGGCAGTGGACTGGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGCCTTCGATCCGCACTGGCCGC..ACGCATGC.CGC..GGCGGTCCCCCCGCgggg                                                                 >  2:2755143/1‑101 (MQ=25)
                                      cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGGGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                               <  1:4501849/101‑1 (MQ=255)
                                       gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                              >  2:388777/1‑101 (MQ=255)
                                         gCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTg                                                            <  1:38598/101‑1 (MQ=255)
                                          cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:3092989/101‑1 (MQ=255)
                                           attgcacgggTCGCTCCCCCCGCGGGGGCGTGGATTGAAACTCTCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTTGCTCCCCCCGCGGGGGCGTGGa                                                          <  2:769882/93‑1 (MQ=37)
                                           aGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTggg                                                          >  1:3026338/1‑100 (MQ=255)
                                             tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:3501152/101‑1 (MQ=255)
                                             ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC...GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  1:1861376/95‑1 (MQ=9)
                                              tgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTGCCTCGATCCGTTC.AGCCTC...GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:5002561/96‑1 (MQ=2)
                                              tgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC...GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:2058501/96‑1 (MQ=9)
                                               gcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC...GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       >  2:1056044/5‑101 (MQ=2)
                                               gACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      <  1:1595402/101‑1 (MQ=255)
                                                 cTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    <  1:1775693/101‑1 (MQ=255)
                                                 cTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    >  2:4675284/1‑101 (MQ=255)
                                                  tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   <  1:893138/101‑1 (MQ=255)
                                                  tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAc                                                   >  1:4741052/1‑101 (MQ=255)
                                                   tgtccccccccccGGGGGGGCGTCGATCGAAACTCCCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                  <  2:3558888/94‑1 (MQ=37)
                                                   gtttgCTCCCCCCGCGTGGGCGTTGATCGAAACTCGCCCTCGATCCGCGCTTGCCCC..ACGCCCTC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                  <  1:3373145/97‑1 (MQ=37)
                                                   ggTCCCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                  <  2:895575/101‑1 (MQ=255)
                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAACCTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                               <  2:395977/101‑1 (MQ=255)
                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                               >  1:2156435/1‑101 (MQ=255)
                                                       gccccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                              <  2:3065657/98‑1 (MQ=255)
                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTCGTATTCCGCG.CTG..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtga                                               >  2:4176948/1‑97 (MQ=2)
                                                        ccccccccGCGGGGGCGTGTATCGAAACTCTCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCTCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                             <  2:177550/99‑1 (MQ=255)
                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                             <  2:4492004/101‑1 (MQ=255)
                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                             >  2:1715640/1‑101 (MQ=255)
                                                         tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC...GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                             >  1:2409037/1‑95 (MQ=2)
                                                          ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCac                                           >  2:1297279/1‑100 (MQ=255)
                                                          ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                           >  1:2798659/1‑101 (MQ=255)
                                                            ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC...GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                          <  1:4878672/101‑10 (MQ=2)
                                                              ccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGa                                       <  2:4332613/101‑1 (MQ=255)
                                                                gcgGGGGCGTGGATCGACACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATg                                     <  2:4538307/101‑1 (MQ=255)
                                                                  gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                   <  1:1690687/101‑1 (MQ=255)
                                                                   ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                  <  1:662823/101‑1 (MQ=255)
                                                                   ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                  <  1:4420703/101‑1 (MQ=255)
                                                                    gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                                 <  1:565828/101‑1 (MQ=255)
                                                                     ggCGTGGATCGAACCTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                <  1:4589430/101‑1 (MQ=255)
                                                                     ggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                <  1:31227/101‑1 (MQ=255)
                                                                     ggCGTGGATCGAAACGCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                <  1:2184365/101‑1 (MQ=255)
                                                                      gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                               <  2:1104671/101‑1 (MQ=255)
                                                                      gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                               >  2:2400665/1‑101 (MQ=255)
                                                                       cGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGc                              <  2:2720318/101‑1 (MQ=255)
                                                                         tGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                            >  2:2545446/1‑101 (MQ=255)
                                                                          ggATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCg                           >  2:7052/1‑101 (MQ=255)
                                                                              cGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACAGGCGCATCTGATGCATATGCCCGGTga                       >  2:4423665/1‑101 (MQ=255)
                                                                              cGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatatagatcggaa                       >  1:3224743/1‑92 (MQ=255)
                                                                              cGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCTGTga                       <  2:1500246/101‑1 (MQ=255)
                                                                              cGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTga                       >  2:3712323/1‑101 (MQ=255)
                                                                               gAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                      <  2:1620685/101‑1 (MQ=255)
                                                                               gAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                      <  1:1765523/101‑1 (MQ=255)
                                                                               gAAACGCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                      <  2:2051547/101‑1 (MQ=255)
                                                                                aaaCTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                     <  2:2290672/101‑1 (MQ=255)
                                                                                aaaCTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                     <  2:2618568/101‑1 (MQ=255)
                                                                                aaaCTCGCCCGCGATCCGCGCTTGCCCC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                     <  1:4830353/101‑1 (MQ=255)
                                                                                 accTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCGCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                    <  2:2194806/99‑1 (MQ=255)
                                                                                 aaCTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                    <  2:3319218/101‑1 (MQ=255)
                                                                                 aaCTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                    <  1:10771/101‑1 (MQ=255)
                                                                                      gCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTg               >  2:1168684/1‑101 (MQ=255)
                                                                                       cccTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt              <  1:3655839/101‑1 (MQ=255)
                                                                                           cGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt          >  2:13508/1‑101 (MQ=255)
                                                                                            gATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTc         <  1:121512/101‑1 (MQ=255)
                                                                                               ccGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc      <  1:4205872/101‑1 (MQ=255)
                                                                                               ccGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc      >  1:232131/1‑101 (MQ=255)
                                                                                                cgcgCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTccccc     <  2:4872164/101‑1 (MQ=255)
                                                                                                 gcgcTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc    <  1:2239181/101‑1 (MQ=255)
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GGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGC  >  NC_014034/1395913‑1396112

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG