Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,156 C→T 3.1% P13L (CCG→TTG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,1560C→T 3.1% 9.5 ‑0.8 ‑0.0224P13L (CCG→CTG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (99/118):  total (101/123)
Fisher's exact test strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.37e-01

Only 100 of 288 total aligned reads displayed.

ATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1396056‑1396256
                                                                                                      |                                                                                                     
   gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcc                                                                                                    >  1:1000279/1‑101 (MQ=255)
   gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcc                                                                                                    <  2:661042/101‑1 (MQ=255)
   gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcc                                                                                                    >  2:4044832/1‑101 (MQ=255)
   gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcc                                                                                                    >  2:1126209/1‑101 (MQ=255)
     aaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAc                                                                                                  <  2:176717/101‑1 (MQ=255)
     aaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAc                                                                                                  <  2:1379331/101‑1 (MQ=255)
       cAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGc                                                                                                >  1:82701/1‑101 (MQ=255)
           cgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCg                                                                                            <  2:1911183/101‑1 (MQ=255)
                cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCt                                                                                       >  2:4791846/1‑101 (MQ=255)
                cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCt                                                                                       <  2:3380768/101‑1 (MQ=255)
                cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGCGGGTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCCGCGCCGcc                                                                                       >  1:1811891/1‑100 (MQ=38)
                 tGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTc                                                                                      >  2:865227/1‑101 (MQ=255)
                  gATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcc                                                                                     <  1:3830874/101‑1 (MQ=255)
                   aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccc                                                                                    <  1:4523217/101‑1 (MQ=255)
                     gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccccc                                                                                  <  2:1108562/101‑1 (MQ=255)
                      tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                                 <  1:2210708/101‑1 (MQ=255)
                      tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                                 <  2:3866397/101‑1 (MQ=255)
                      tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCCCTcccccc                                                                                 >  2:4148678/1‑101 (MQ=255)
                      tataCGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                                 <  1:4204241/101‑1 (MQ=255)
                         atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCCCTGCGTCGCTCCCCCcccg                                                                              >  1:3774308/1‑101 (MQ=255)
                         atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCcgcg                                                                              >  2:4881231/1‑101 (MQ=255)
                         atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACCCTGCGCCGCTCCCCCcgcg                                                                              >  1:1373032/1‑101 (MQ=255)
                          tGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCgg                                                                             >  1:2013274/1‑101 (MQ=255)
                           gCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCggg                                                                            >  2:2714897/1‑101 (MQ=255)
                             ccGGTGAGCCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCggggg                                                                          <  1:978965/101‑1 (MQ=255)
                               ggTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCg                                                                        <  2:3504587/101‑1 (MQ=255)
                                gTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGt                                                                       <  1:3712601/101‑1 (MQ=255)
                                 tgtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                      >  1:3125486/11‑101 (MQ=9)
                                 tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCggg                                                                      >  1:928894/1‑99 (MQ=255)
                                 tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCCGCGTCGCTCCCCCCCCGGGGGCggg                                                                      >  1:5053578/1‑99 (MQ=255)
                                  gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  1:4299983/101‑1 (MQ=255)
                                  gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGgg                                                                     >  1:734142/1‑101 (MQ=255)
                                   agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCCCGGGGGCGTggg                                                                    >  1:3178220/1‑100 (MQ=255)
                                   agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCCCGGGGGCGTGGa                                                                    >  1:3883656/1‑101 (MQ=255)
                                    gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  2:4857267/101‑1 (MQ=255)
                                     aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  1:3538387/101‑1 (MQ=255)
                                      cGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 >  2:5046488/1‑101 (MQ=255)
                                         cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.aaa                                                             <  2:2956625/101‑1 (MQ=255)
                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAAcc                                                           >  2:3016838/1‑101 (MQ=255)
                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGCGGGCGCGGAACG.AAAcc                                                           >  2:2469481/1‑101 (MQ=255)
                                            tCGCTACCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAAccc                                                          >  2:35579/1‑101 (MQ=255)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCg                                                         >  1:3407938/1‑101 (MQ=255)
                                                 ccccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.GAGGCTCGCGACGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGa                                                     <  1:5027604/101‑1 (MQ=255)
                                                    cccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaaa                                                  <  2:911137/101‑1 (MQ=255)
                                                    cccGCGGGGGCGTGGATCGAAACTTTTCGGC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaaa                                                  <  2:4546022/101‑1 (MQ=255)
                                                     ccGCGGGGGCGTGGATCGAAACTTTTCGTG.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCCCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaaaa                                                 <  2:4157104/101‑1 (MQ=255)
                                                     ccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAACCCGGccacca                                                  >  2:2375979/1‑96 (MQ=38)
                                                       gcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACg                                               <  2:4976597/101‑1 (MQ=255)
                                                         gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGc                                             >  1:3019027/1‑101 (MQ=255)
                                                         gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGc                                             <  2:4460290/101‑1 (MQ=255)
                                                          ggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGcc                                            >  2:1543604/1‑101 (MQ=255)
                                                          ggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCAGGGGCGTGGATCG.AAACCCGGCGAAAAACGGcc                                            <  2:2156287/101‑1 (MQ=255)
                                                           gggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCG.AAACCCGGCGAAAAACGGCCa                                           >  2:3665901/1‑101 (MQ=255)
                                                            ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCAt                                          >  1:4400940/1‑101 (MQ=255)
                                                             gCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATg                                         <  1:4155837/101‑1 (MQ=255)
                                                              cGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGc                                        <  2:4245240/101‑1 (MQ=255)
                                                              cGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGc                                        >  2:2592344/1‑101 (MQ=255)
                                                               gTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGcc                                       >  1:2767650/1‑101 (MQ=255)
                                                                tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGccc                                      >  1:3251092/1‑100 (MQ=255)
                                                                tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCt                                      <  1:4424082/101‑1 (MQ=255)
                                                                tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCt                                      >  1:4096346/1‑101 (MQ=255)
                                                                 ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCCTGCCTc                                     >  1:2748803/1‑101 (MQ=255)
                                                                 ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTc                                     >  2:3184404/1‑101 (MQ=255)
                                                                 ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTc                                     >  1:2858925/1‑101 (MQ=255)
                                                                  gATCGAAACTTTTCTTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTcc                                    <  2:3730829/101‑1 (MQ=255)
                                                                  gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTcc                                    <  1:4481155/101‑1 (MQ=255)
                                                                  gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTcc                                    >  1:1305065/1‑101 (MQ=255)
                                                                   aTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCg                                   <  1:4677098/101‑1 (MQ=255)
                                                                   aTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCg                                   <  2:82691/101‑1 (MQ=255)
                                                                    tCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCgg                                  <  1:4016205/101‑1 (MQ=255)
                                                                      gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCgggg                                >  2:2053838/1‑100 (MQ=255)
                                                                      gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGgt                                <  2:567644/101‑1 (MQ=255)
                                                                        aaCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGgtgt                              <  1:605851/101‑1 (MQ=255)
                                                                         aCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTc                             >  2:2559239/1‑101 (MQ=255)
                                                                          cTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCt                            >  1:2674950/1‑101 (MQ=255)
                                                                           ttttCGTT.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                           <  1:1113140/101‑1 (MQ=255)
                                                                           ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                           >  1:2506927/1‑101 (MQ=255)
                                                                            tttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg                          >  1:103811/1‑101 (MQ=255)
                                                                            tttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg                          >  2:3075802/1‑101 (MQ=255)
                                                                            tttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg                          >  2:2092674/1‑101 (MQ=255)
                                                                             ttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGc                         >  2:3264667/1‑101 (MQ=255)
                                                                             ttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGc                         <  2:277114/101‑1 (MQ=255)
                                                                              tCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCt                        >  1:3336901/1‑101 (MQ=255)
                                                                                  c.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                    <  1:3674647/101‑1 (MQ=255)
                                                                                  c.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                    <  1:4948492/101‑1 (MQ=255)
                                                                                  c.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCTGGCTGTCg                    >  2:2301198/1‑101 (MQ=255)
                                                                                  c.ATCTGG.CAGGCGCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                    <  1:1685185/101‑1 (MQ=255)
                                                                                    aTCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGc                   >  2:4650676/1‑101 (MQ=255)
                                                                                      cTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTc                 <  2:3710130/101‑1 (MQ=255)
                                                                                      cTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCTGGCTGTCGCTc                 >  2:1824596/1‑101 (MQ=255)
                                                                                      cTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCCGGCTGTCGCTc                 >  1:1711377/1‑101 (MQ=255)
                                                                                        gg.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccc               >  2:43826/1‑101 (MQ=255)
                                                                                        gg.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccc               >  2:2497653/1‑101 (MQ=255)
                                                                                           cAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccccc             <  1:3683922/101‑1 (MQ=255)
                                                                                            aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc            <  1:1168801/101‑1 (MQ=255)
                                                                                            aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCTGGCTGTCGCTcccccc            >  2:1665496/1‑101 (MQ=255)
                                                                                              gCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcgc          >  1:4768231/1‑101 (MQ=255)
                                                                                                 cgcgCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCggg       >  1:1781379/1‑101 (MQ=255)
                                                                                                    gccgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGc    >  1:2334568/1‑101 (MQ=255)
                                                                                                      cgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGt  >  2:766285/1‑101 (MQ=255)
                                                                                                      |                                                                                                     
ATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1396056‑1396256

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG