BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,835	G→C	13.3%	W239C (TGG→TGC)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,835	0	G→C	13.3%	96.9 ‑3.0 ‑0.0	323	W239C (TGG→TGC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (13/30): ref base (132/148): total (145/178)
Fisher's exact test strand distribution p-value = 4.76e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.77e-01

Only 100 of 342 total aligned reads displayed.


AACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA  >  NC_014034/1396735‑1396935


aaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGGGCGGACGGCTTgg                                                                                                      >  1:2858969/1‑101 (MQ=255)


aaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTgg                                                                                                      <  1:2683431/101‑1 (MQ=255)


   ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTc                                                                                                   >  2:3328727/1‑101 (MQ=255)


    cTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCg                                                                                                  >  1:3217629/1‑101 (MQ=255)


     tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGc                                                                                                 >  1:2033061/1‑101 (MQ=255)


      gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCt                                                                                                >  2:4723758/1‑101 (MQ=255)


      gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGGCGcc                                                                                                >  1:3427231/1‑100 (MQ=255)


       tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTc                                                                                               <  1:174666/101‑1 (MQ=255)


       tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTc                                                                                               >  2:2645846/1‑101 (MQ=255)


       tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTc                                                                                               <  2:3011277/101‑1 (MQ=255)


         ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccc                                                                                             >  1:612029/1‑101 (MQ=255)


            cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccccc                                                                                          >  1:1121779/1‑101 (MQ=255)


             atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcg                                                                                         <  1:1428145/101‑1 (MQ=255)


             atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcg                                                                                         <  2:3885875/101‑1 (MQ=255)


              tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcgc                                                                                        >  2:2135574/1‑101 (MQ=255)


              tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcgc                                                                                        <  1:1053093/101‑1 (MQ=255)


                tgctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGCCGGCTTGGGGCCCCCCCCCCCCgg                                                                                      >  2:4078979/1‑101 (MQ=37)


                 gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggg                                                                                     <  1:4995250/101‑1 (MQ=255)


                 gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggg                                                                                     >  2:3591349/1‑101 (MQ=255)


                   tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggggg                                                                                   >  2:1612716/1‑101 (MQ=255)


                    gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGc                                                                                  <  2:2603580/101‑1 (MQ=255)


                        cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTgg                                                                              <  1:3339752/101‑1 (MQ=255)


                         gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                             <  2:1148679/101‑1 (MQ=255)


                         gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGGGGa                                                                             >  1:2800565/1‑101 (MQ=255)


                          tcacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                              <  1:3829281/91‑1 (MQ=2)


                          tcacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                              >  1:365564/11‑101 (MQ=2)


                          aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTgggt                                                                            >  2:1502468/1‑99 (MQ=255)


                            gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGCCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                          >  1:4379922/1‑101 (MQ=255)


                             caccgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                           <  2:4808016/94‑1 (MQ=2)


                              ctcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                          <  2:846804/95‑1 (MQ=2)


                              ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                        >  2:1222779/1‑101 (MQ=255)


                              atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                          >  2:904771/7‑101 (MQ=2)


                               tcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                         <  2:241801/96‑1 (MQ=2)


                               cTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                       <  2:4986940/101‑1 (MQ=255)


                                ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                      <  1:1247977/101‑1 (MQ=255)


                                ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                      <  1:4676019/101‑1 (MQ=255)


                                 tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                     <  1:4757422/101‑1 (MQ=255)


                                 tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                     >  2:3114757/1‑101 (MQ=255)


                                  gAGNCGNTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                                    <  1:721246/101‑1 (MQ=255)


                                  ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                      <  1:1079896/99‑1 (MQ=2)


                                   cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                                     >  2:1690500/2‑100 (MQ=2)


                                   attCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgg                                                                   <  1:2457743/99‑1 (MQ=255)


                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCGTGCAGCGCGGACGGCTTGGGGTGTCCCCCCCGCGGGGGGGGGGGGCGAAAACggc                                                                  >  2:349519/1‑100 (MQ=21)


                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                                   <  1:2430041/101‑4 (MQ=2)


                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatgg                                                                  <  2:2822836/101‑5 (MQ=2)


                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                                                >  1:2903807/1‑101 (MQ=255)


                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                                               >  1:1555754/1‑101 (MQ=255)


                                        cTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCag                                                              >  2:2755367/1‑101 (MQ=255)


                                        cTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCag                                                              <  2:1462202/101‑1 (MQ=255)


                                          ccccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                                              <  1:3974789/101‑9 (MQ=2)


                                              ccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                                        <  1:3591620/101‑1 (MQ=255)


                                              ccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                                        <  1:4724081/101‑1 (MQ=255)


                                              ccGCGGGGGCGGGGATCGAAACCGTGTGGAT...GAGCAGGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                                        <  2:2384965/101‑1 (MQ=255)


                                                gcgTGGACGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGTTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAc                                                      <  2:2439453/101‑1 (MQ=255)


                                                gcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAc                                                      <  1:2112734/101‑1 (MQ=255)


                                                 cgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                                                     <  1:4570275/101‑1 (MQ=255)


                                                   ggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                                                   <  2:4556829/101‑1 (MQ=255)


                                                     ggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgc                                                 <  2:4097229/101‑1 (MQ=255)


                                                       cGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgg                                               <  1:1363367/101‑1 (MQ=255)


                                                        gTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                                              >  1:1631046/1‑101 (MQ=255)


                                                        gTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                                              >  2:3141608/1‑101 (MQ=255)


                                                         tGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                                             >  1:4272421/1‑101 (MQ=255)


                                                             tCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcc                                         <  1:1509934/101‑1 (MQ=255)


                                                              cGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGACACCGGTCCAGAGATAGGCCGCGGGGAcctcct                                        >  2:3985007/1‑101 (MQ=255)


                                                              cGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                                        <  1:4897756/101‑1 (MQ=255)


                                                               gAACCCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                                       <  2:3400520/101‑1 (MQ=255)


                                                                  aCCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGa                                    <  2:6119/101‑1 (MQ=255)


                                                                    cGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg                                  <  1:1146882/101‑1 (MQ=255)


                                                                     gtgtGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGt                                 >  2:634113/1‑101 (MQ=255)


                                                                      tgtgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCACCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTc                                >  2:1791655/1‑101 (MQ=255)


                                                                       gtgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg                               >  1:1838987/1‑101 (MQ=255)


                                                                       gtgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg                               >  2:1391881/1‑101 (MQ=255)


                                                                       gtgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg                               >  1:2173820/1‑101 (MQ=255)


                                                                        tgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGc                              <  2:4645127/101‑1 (MQ=255)


                                                                         ggAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAACGGTCCAGAGATAGAACGCGCGGACCTCCTCAGACGTCGCt                             >  1:2825909/1‑101 (MQ=255)


                                                                            t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc                          <  1:4011848/101‑1 (MQ=255)


                                                                                gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccc                         <  1:4079282/101‑1 (MQ=255)


                                                                                 atCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccccc                        <  1:3762819/101‑1 (MQ=255)


                                                                                  tCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTTAGACGTCGCGctcccc                       >  1:818568/1‑101 (MQ=255)


                                                                                  tCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccccc                       <  1:936406/101‑1 (MQ=255)


                                                                                   cATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGTGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCcg                      <  1:3928471/101‑1 (MQ=255)


                                                                                        aGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCgggg                 >  1:4168925/1‑101 (MQ=255)


                                                                                         gcgcGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCggggg                <  1:4106466/101‑1 (MQ=255)


                                                                                         gcgcGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCggggg                <  1:4617911/101‑1 (MQ=255)


                                                                                           gcgGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCg              >  2:2874339/1‑101 (MQ=255)


                                                                                           gcgGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCg              >  1:228463/1‑101 (MQ=255)


                                                                                             ggACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTg            >  2:2549101/1‑101 (MQ=255)


                                                                                              gACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTgg           >  2:2251473/1‑101 (MQ=255)


                                                                                              gACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTgg           >  1:3504239/1‑101 (MQ=255)


                                                                                               aCGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGa          <  2:4028637/101‑1 (MQ=255)


                                                                                               aCGGCTTGGGTCACTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGa          >  1:313964/1‑101 (MQ=255)


                                                                                                   cTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa      >  1:3296256/1‑101 (MQ=255)


                                                                                                   cTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa      >  1:1936999/1‑101 (MQ=255)


                                                                                                    ttGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     <  2:4299501/101‑1 (MQ=255)


                                                                                                    ttGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     <  2:1925991/101‑1 (MQ=255)


                                                                                                    ttGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     >  2:1854192/1‑101 (MQ=255)


                                                                                                    ttGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     >  1:3053883/1‑101 (MQ=255)


                                                                                                    ttGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGGCCAGAGATAGACCGCGCGGACCTCCCCACCCGTCCCCACCCCCGCGGGCGCGTGGATCGaa     >  1:2018858/1‑101 (MQ=25)


                                                                                                    tggggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     <  2:4180123/99‑1 (MQ=255)


                                                                                                      gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc   >  2:3750419/1‑101 (MQ=255)


AACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA  >  NC_014034/1396735‑1396935

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG