BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,951	T→A	12.3%	I278N (ATC→GAC)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,951	0	T→A	12.3%	127.4 ‑1.1 ‑0.5	414	I278N (ATC→AAC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (27/20): ref base (158/163): total (209/205)
Fisher's exact test strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.26e-01

Only 100 of 452 total aligned reads displayed.


GGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGG  >  NC_014034/1396851‑1397051


 gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.Tc                                                                                                            <  2:1724295/101‑1 (MQ=255)


 gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.Tc                                                                                                            <  2:2157452/101‑1 (MQ=255)


    cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGc                                                                                                         >  2:3322895/1‑101 (MQ=255)


     gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCt                                                                                                        >  2:2364252/1‑101 (MQ=255)


      tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCt.t                                                                                                      >  2:958840/1‑101 (MQ=255)


      tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCt.t                                                                                                      <  1:1484668/101‑1 (MQ=255)


       ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.Tc                                                                                                     >  1:4839750/1‑101 (MQ=255)


        gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.Tcc                                                                                                    <  1:1788990/101‑1 (MQ=255)


         aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCg                                                                                                   >  2:4124382/1‑101 (MQ=255)


             aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..g                                                                                            >  2:1988327/1‑101 (MQ=255)


              aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..c                                                                                         >  1:4145564/1‑101 (MQ=255)


               aCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..Cg                                                                                        >  2:1096149/1‑101 (MQ=255)


                 cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTg                                                                                      >  1:3662621/1‑101 (MQ=255)


                   gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CC..G..CGTG.Gt                                                                                   >  1:2327877/1‑101 (MQ=255)


                       agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCt                                                                               >  2:4417943/1‑101 (MQ=255)


                           aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..C..CGTG.GGCGCTcccc                                                                           >  2:1226999/1‑101 (MQ=255)


                            tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCCC.CT..G..CGTG.GTCGCTccccc                                                                          >  1:3955416/1‑101 (MQ=255)


                             aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTcccccc                                                                         <  2:228429/101‑1 (MQ=255)


                             aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTcccccc                                                                         >  2:3407247/1‑101 (MQ=255)


                                     cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..C..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                 >  1:2130045/1‑101 (MQ=255)


                                      ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                                >  2:1847137/1‑101 (MQ=255)


                                         cctcctCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CCCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  2:4993851/101‑1 (MQ=255)


                                          tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.GC.AGTG.TCAGGT.T.TTC.CA..G..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  1:4696864/92‑1 (MQ=0)


                                          tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.GC.AGTG.TCAGGT.T.TTC.CA..G..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  2:4033074/92‑1 (MQ=0)


                                          tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.GC.AGTG.TCAGGT.T.TTC.CA..G..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  2:1455824/92‑1 (MQ=0)


                                          ggtcaacactgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  2:3587270/91‑1 (MQ=9)


                                           ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                           >  1:4088813/10‑101 (MQ=2)


                                            tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:3217404/93‑1 (MQ=2)


                                            cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          >  2:2606539/1‑101 (MQ=255)


                                            cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:936471/101‑1 (MQ=255)


                                             ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  2:3027319/94‑1 (MQ=2)


                                             ctcAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCATGGATCg                                                         <  2:3920075/101‑1 (MQ=255)


                                              tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        <  2:1290338/101‑1 (MQ=255)


                                              ggttgcGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.GC.AGTG.TCAGGT.T.TTC.CA..G..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  2:1666920/96‑1 (MQ=2)


                                               cgcccggcGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  2:4786259/94‑1 (MQ=2)


                                               cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       <  1:1845182/101‑1 (MQ=255)


                                               cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       <  2:3157727/101‑1 (MQ=255)


                                               cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                                       >  1:635910/1‑101 (MQ=255)


                                               acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        >  1:1521890/6‑101 (MQ=9)


                                                aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGGGGGGGTCGaaa                                                      >  1:20549/1‑101 (MQ=255)


                                                aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                                      >  1:429884/1‑101 (MQ=255)


                                                 tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.GC.AGTG.TCAGGT.T.TTC.CA..G..CGGC.GTCGCTCCCCCCCCGGGGGCGTGGGTCGaaa                                                      >  1:4887194/3‑101 (MQ=2)


                                                 tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.GC.AGTG.TCAGGT.T.TTC.CA..G..CGGC.GGCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      >  2:290430/3‑101 (MQ=2)


                                                  gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.GC.AGTG.TCAGGT.T.TTC.CA..G..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     <  1:716399/100‑1 (MQ=2)


                                                  cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                    <  2:4723137/99‑2 (MQ=2)


                                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                   <  1:4972877/101‑1 (MQ=255)


                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATc                                                  >  2:1004889/1‑101 (MQ=255)


                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                                  >  1:3824646/1‑98 (MQ=2)


                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAActc                                                   >  1:1057255/1‑99 (MQ=9)


                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAActc                                                   >  1:1664245/1‑99 (MQ=9)


                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCa                                                 >  1:3577883/1‑101 (MQ=255)


                                                     tCGCTCCACCCGCGGGGTCGTGGGCCGAAACA.ACGCG.GCGCG.GG.CCTA.T.GGCTGTCGCC.CC..C..CCCC.GTCGCTCCCCCCCCGGGGGCGTGGATCGAAActgc                                                  <  2:710065/101‑4 (MQ=2)


                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                   >  1:1221739/1‑100 (MQ=2)


                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCC.CCGGATCTTTGT.CT..G..CGCG.GGCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                                 >  1:2235468/1‑97 (MQ=9)


                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                               <  1:44290/101‑7 (MQ=2)


                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                               >  1:3393917/1‑95 (MQ=2)


                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                >  1:1927665/1‑96 (MQ=9)


                                                       ccTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                                  <  2:3235949/100‑3 (MQ=2)


                                                       ccTCCCCCCGCGGGGGCGGGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCacc                                               <  2:4909765/100‑3 (MQ=255)


                                                        ccccccccGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                                 <  1:4471075/99‑1 (MQ=2)


                                                        cacccccgGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCt                                              <  1:4995760/99‑1 (MQ=255)


                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                                 <  1:4542812/101‑1 (MQ=2)


                                                        cTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                               <  1:2835879/101‑7 (MQ=9)


                                                         cccccccGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                             <  2:2759261/100‑1 (MQ=255)


                                                          ccccccGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                               <  1:2645404/101‑3 (MQ=2)


                                                          ccccccGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA.ACGGCC.TATGCGCTGGG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                            >  1:4245610/1‑92 (MQ=2)


                                                          ccccccGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                            <  2:4469526/101‑10 (MQ=2)


                                                          ccccccGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                            >  2:3062559/1‑92 (MQ=2)


                                                           cccccGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                            <  1:2414911/101‑10 (MQ=9)


                                                             cccGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                         >  1:1305278/1‑101 (MQ=255)


                                                              ccGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                           <  1:2469531/101‑7 (MQ=0)


                                                              ccGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcgtcgt                                        <  1:2943651/101‑1 (MQ=255)


                                                               cgcgGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                          <  2:3670359/101‑8 (MQ=0)


                                                               cgcgGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                       <  1:3333269/101‑1 (MQ=255)


                                                                gggggggCGTGGATCGAAACA.GC.CT.GCGCG.GC.CCCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                      <  1:2195171/99‑1 (MQ=255)


                                                                gcgGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGATg                                      >  2:4255975/1‑101 (MQ=255)


                                                                 cgGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                     <  1:1394581/101‑1 (MQ=255)


                                                                  gggggCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                    <  2:2651635/101‑1 (MQ=255)


                                                                      gCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                                >  2:779099/1‑101 (MQ=255)


                                                                       gggggATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                               <  2:1504348/98‑1 (MQ=255)


                                                                       cGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                               <  1:3496113/101‑1 (MQ=255)


                                                                        gTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                              >  1:1970120/1‑101 (MQ=255)


                                                                          ggATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                            <  2:3755266/101‑1 (MQ=255)


                                                                          ggATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                            >  1:4006472/1‑101 (MQ=255)


                                                                          ggATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                            >  2:1399203/1‑101 (MQ=255)


                                                                          ggATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                            >  1:3777720/1‑101 (MQ=255)


                                                                            aTCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                          >  1:2718795/1‑101 (MQ=255)


                                                                               gAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                       <  1:771636/101‑1 (MQ=255)


                                                                               gAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                       >  1:309201/1‑101 (MQ=255)


                                                                                aaaCA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                      >  2:4302216/1‑101 (MQ=255)


                                                                                 aaCA.GC.CT.GGGCG.GC.CTCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                     <  1:1468078/101‑1 (MQ=255)


                                                                                  aCA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc                    >  2:1913806/1‑101 (MQ=255)


                                                                                      gC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc                 <  2:2825696/101‑1 (MQ=255)


                                                                                            gcgcG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc             >  2:1358561/1‑101 (MQ=255)


                                                                                                g.gC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc         >  2:1131640/1‑101 (MQ=255)


                                                                                                  gC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc        <  1:2897020/101‑1 (MQ=255)


                                                                                                      gCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg     >  2:4655587/1‑101 (MQ=255)


                                                                                                      gCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg     >  1:3733803/1‑101 (MQ=255)


                                                                                                       cA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgg    >  1:2300340/1‑101 (MQ=255)


                                                                                                        a.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg   >  2:56684/1‑101 (MQ=255)


GGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGG  >  NC_014034/1396851‑1397051

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG