BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,149	T→C	5.0%	M344T (ATG→ACG)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,149	0	T→C	5.0%	18.8 ‑0.9 ‑0.0	259	M344T (ATG→ACG)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (5/8): ref base (126/120): total (131/128)
Fisher's exact test strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.80e-01

Only 100 of 278 total aligned reads displayed.


GGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGG  >  NC_014034/1397049‑1397249


ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                                       >  1:3084085/1‑101 (MQ=255)


   gCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTc                                                                                                    <  2:2107741/101‑1 (MQ=255)


     gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                                  >  2:4283275/1‑101 (MQ=255)


      tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                                 <  2:3671866/101‑1 (MQ=255)


      tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                                 >  1:823525/1‑101 (MQ=255)


      gggATCGCAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                                 <  2:1195865/100‑1 (MQ=255)


       ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                                >  1:2652318/1‑101 (MQ=255)


          tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc                                                                                             <  1:1233753/101‑1 (MQ=255)


          tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc                                                                                             <  1:1358687/101‑1 (MQ=255)


          tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc                                                                                             >  2:3147732/1‑101 (MQ=255)


           cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                                           >  1:2711073/1‑101 (MQ=255)


            gAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.Gt                                                                                          >  1:2571271/1‑101 (MQ=255)


            gAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.Gt                                                                                          <  1:4423360/101‑1 (MQ=255)


             aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTg                                                                                         <  2:2173665/101‑1 (MQ=255)


             aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTg                                                                                         >  1:981295/1‑101 (MQ=255)


              aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgc                                                                                        <  1:3750697/101‑1 (MQ=255)


              aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgc                                                                                        <  1:13900/101‑1 (MQ=255)


               aCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgcg                                                                                       >  2:2089958/1‑101 (MQ=255)


                  gCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCg                                                                                    >  2:4980865/1‑101 (MQ=255)


                    cGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTc                                                                                  >  1:713399/1‑101 (MQ=255)


                      tCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGc                                                                                >  1:1603780/1‑101 (MQ=255)


                        ttGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTc                                                                              >  2:1513187/1‑101 (MQ=255)


                        ttGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTc                                                                              >  1:4250795/1‑101 (MQ=255)


                        ttGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTc                                                                              >  2:460722/1‑101 (MQ=255)


                         tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcc                                                                             >  1:1924204/1‑101 (MQ=255)


                           aGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccc                                                                           <  2:230010/101‑1 (MQ=255)


                            gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccccc                                                                          <  1:3373009/101‑1 (MQ=255)


                            gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccccc                                                                          <  2:338701/101‑1 (MQ=255)


                             tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCCGCTCCCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                                         >  2:2018752/1‑101 (MQ=255)


                             tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                                         <  1:3402017/101‑1 (MQ=255)


                             tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGGCTTAGACC.GTGCGCCGTCGCTcccccc                                                                         >  2:4561740/1‑101 (MQ=255)


                              gATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                        <  1:3244958/101‑1 (MQ=255)


                              gATGCGTCGCGAGCACATGTCGTCAATCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                        >  2:157543/1‑101 (MQ=38)


                                 ggggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCgg                                                                     >  1:2818280/1‑101 (MQ=255)


                                 ggggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCgg                                                                     <  2:3828343/101‑1 (MQ=255)


                                  gggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCggg                                                                    >  2:1729476/1‑101 (MQ=255)


                                  gggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCggg                                                                    >  1:4865788/1‑101 (MQ=255)


                                     cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGc                                                                 <  1:2166127/101‑1 (MQ=255)


                                     cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGc                                                                 <  2:3871027/101‑1 (MQ=255)


                                      gcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCCCGGGGGCg                                                                >  2:2651432/1‑101 (MQ=255)


                                        gAGCACATGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                              <  1:1004999/101‑1 (MQ=255)


                                         aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                             <  2:4690403/101‑1 (MQ=255)


                                         aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                             <  1:439382/101‑1 (MQ=255)


                                           cggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  1:54187/93‑1 (MQ=0)


                                           cggctgcggtcgCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  2:206686/93‑1 (MQ=0)


                                           cacaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  2:4006170/101‑1 (MQ=255)


                                             gctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  1:1007183/7‑101 (MQ=0)


                                             caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  1:3945927/1‑101 (MQ=255)


                                              ctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        <  2:2718213/96‑1 (MQ=2)


                                              aTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        >  2:2730960/1‑101 (MQ=255)


                                               tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  2:1840076/5‑101 (MQ=2)


                                               tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       <  1:3896980/101‑1 (MQ=255)


                                                gcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      >  1:1266709/4‑101 (MQ=2)


                                                 tcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     <  2:573628/101‑1 (MQ=255)


                                                  ggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                    >  1:4112736/2‑100 (MQ=2)


                                                  cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                    >  2:4412395/1‑101 (MQ=255)


                                                   gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgt                                                   <  2:1967695/101‑1 (MQ=255)


                                                    tcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtg                                                  >  1:3625825/1‑101 (MQ=255)


                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                                <  1:460787/101‑1 (MQ=255)


                                                       ccccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                               <  2:3627888/99‑1 (MQ=255)


                                                         ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                                             <  2:683279/101‑1 (MQ=255)


                                                          cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                                            <  1:1824189/101‑1 (MQ=255)


                                                           ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                           <  1:1513317/101‑1 (MQ=255)


                                                           ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                           <  1:2090106/101‑1 (MQ=255)


                                                             ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacg                                         <  1:2409380/101‑1 (MQ=255)


                                                               gcgGGGGCGTGGATCGAATCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                                       <  1:2018896/101‑1 (MQ=255)


                                                                cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgacg                                      <  2:3303889/101‑1 (MQ=255)


                                                                cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgacg                                      >  2:5015531/1‑101 (MQ=255)


                                                                cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACCTGTCTGGCacgacg                                      >  1:1101572/1‑101 (MQ=255)


                                                                 gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGc                                     <  1:300419/101‑1 (MQ=255)


                                                                    ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                                  <  2:3031843/101‑1 (MQ=255)


                                                                    ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                                  <  2:4250478/101‑1 (MQ=255)


                                                                      cGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGc                                >  2:2469609/1‑101 (MQ=255)


                                                                       gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCa                               <  2:641389/101‑1 (MQ=255)


                                                                        tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGCCGCCCTGCAc                              >  2:4490740/1‑101 (MQ=255)


                                                                        tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAc                              <  2:2505472/101‑1 (MQ=255)


                                                                         ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc                             >  2:4822559/1‑101 (MQ=255)


                                                                         ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc                             >  2:1794322/1‑101 (MQ=255)


                                                                           aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAg                           <  2:1626791/101‑1 (MQ=255)


                                                                             ctaaaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt                         <  2:137805/99‑1 (MQ=255)


                                                                             cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt                         >  2:2934531/1‑101 (MQ=255)


                                                                              gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCCGCACCAGATc                        >  2:3728104/1‑101 (MQ=255)


                                                                              gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCCGCCACGACGCCCTGCACCAGATc                        <  1:4063160/101‑1 (MQ=255)


                                                                                aaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcg                      >  1:1659312/1‑101 (MQ=255)


                                                                                aaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcg                      >  2:2336368/1‑101 (MQ=255)


                                                                                aaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcg                      <  2:3055253/101‑1 (MQ=255)


                                                                                  cGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcgcg                    >  1:3836747/1‑101 (MQ=255)


                                                                                   gTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCgcgc                   >  1:4826760/1‑101 (MQ=255)


                                                                                      cAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGt                <  2:4365905/101‑1 (MQ=255)


                                                                                       aGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTTCACCAGATCCGCGCCGTc               <  2:1101458/101‑1 (MQ=255)


                                                                                       aGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTc               >  2:4118641/1‑101 (MQ=255)


                                                                                       aGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTc               <  2:4090471/101‑1 (MQ=255)


                                                                                         cTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGc             >  1:4535555/1‑101 (MQ=255)


                                                                                          tCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCt            >  1:2883658/1‑101 (MQ=255)


                                                                                           cgcgCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTc           <  2:1924067/101‑1 (MQ=255)


                                                                                                cacaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTcccccc      >  1:2310744/1‑101 (MQ=255)


                                                                                                cacaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTcccccc      <  2:1011627/101‑1 (MQ=255)


                                                                                                  caTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcgc    >  1:3577472/1‑101 (MQ=255)


                                                                                                   aTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcgcg   >  2:375110/1‑101 (MQ=255)


                                                                                                    tGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACACGATCCGCCCCGTCGCTCCCCCCGCgg  >  2:3438044/1‑101 (MQ=255)


GGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGG  >  NC_014034/1397049‑1397249

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG